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MX377780B - Sobreexpresion de reguladores de la ruta de n-glicosilacion para modular la glicosilacion de proteinas recombinantes. - Google Patents

Sobreexpresion de reguladores de la ruta de n-glicosilacion para modular la glicosilacion de proteinas recombinantes.

Info

Publication number
MX377780B
MX377780B MX2016009911A MX2016009911A MX377780B MX 377780 B MX377780 B MX 377780B MX 2016009911 A MX2016009911 A MX 2016009911A MX 2016009911 A MX2016009911 A MX 2016009911A MX 377780 B MX377780 B MX 377780B
Authority
MX
Mexico
Prior art keywords
glycosylation
overexpression
recombinant proteins
modulate
pathway regulators
Prior art date
Application number
MX2016009911A
Other languages
English (en)
Other versions
MX2016009911A (es
Inventor
Shivani Gupta
Sohye Kang
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53757626&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX377780(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of MX2016009911A publication Critical patent/MX2016009911A/es
Publication of MX377780B publication Critical patent/MX377780B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01143—Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01094—Protein N-acetylglucosaminyltransferase (2.4.1.94)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invención se refiere a métodos para modular las propiedades de un cultivo de células que expresa una proteína de interés. En varias modalidades los métodos se refieren a la sobreexpresión de las proteínas implicadas en la ruta de N-glicosilación.
MX2016009911A 2014-01-29 2014-12-11 Sobreexpresion de reguladores de la ruta de n-glicosilacion para modular la glicosilacion de proteinas recombinantes. MX377780B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461933137P 2014-01-29 2014-01-29
PCT/US2014/069744 WO2015116315A1 (en) 2014-01-29 2014-12-11 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

Publications (2)

Publication Number Publication Date
MX2016009911A MX2016009911A (es) 2016-10-28
MX377780B true MX377780B (es) 2025-03-11

Family

ID=53757626

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2016009911A MX377780B (es) 2014-01-29 2014-12-11 Sobreexpresion de reguladores de la ruta de n-glicosilacion para modular la glicosilacion de proteinas recombinantes.
MX2020012356A MX2020012356A (es) 2014-01-29 2016-07-29 Sobreexpresion de reguladores de la ruta de n-glicosilacion para modular la glicosilacion de proteinas recombinantes.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2020012356A MX2020012356A (es) 2014-01-29 2016-07-29 Sobreexpresion de reguladores de la ruta de n-glicosilacion para modular la glicosilacion de proteinas recombinantes.

Country Status (14)

Country Link
US (3) US10227627B2 (es)
EP (1) EP3099806A1 (es)
JP (4) JP6474420B2 (es)
KR (3) KR102519540B1 (es)
CN (2) CN106170554B (es)
AU (3) AU2014380174B2 (es)
BR (1) BR112016017606A2 (es)
CA (1) CA2938079C (es)
CL (1) CL2016001900A1 (es)
EA (2) EA202190244A3 (es)
IL (3) IL282517B (es)
MX (2) MX377780B (es)
SG (1) SG10201912081RA (es)
WO (1) WO2015116315A1 (es)

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KR101936049B1 (ko) 2015-10-15 2019-01-08 (주)알테오젠 IgG Fc 도메인을 가지는 융합 단백질의 생산방법
EP3596225A1 (en) * 2017-03-14 2020-01-22 Amgen Inc. Control of total afucosylated glycoforms of antibodies produced in cell culture
IL278211B1 (en) 2018-05-01 2023-10-01 Amgen Inc Antibodies with modulated glycan profiles
CN109576212B (zh) * 2018-12-14 2021-06-22 杭州奕安济世生物药业有限公司 高密度接种培养中种子细胞的培养方法及其应用
IL307540A (en) 2021-04-23 2023-12-01 Amgen Inc Anti-TSLP antibody preparations and their uses
CN113684322B (zh) * 2021-09-09 2024-01-23 上海药明生物技术有限公司 一种降低抗体类蛋白高聚甘露糖型水平的方法
CN114525252B (zh) * 2022-03-10 2023-12-01 广州源井生物科技有限公司 一种提高mda-mb-231单细胞克隆形成率的单克隆增强培养基与培养方法及其应用
WO2024092064A1 (en) 2022-10-26 2024-05-02 Amgen Inc. Anti-tslp antibody compositions and uses thereof

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Also Published As

Publication number Publication date
AU2014380174A1 (en) 2016-08-11
KR20220086703A (ko) 2022-06-23
KR102410393B1 (ko) 2022-06-16
MX2016009911A (es) 2016-10-28
IL246914B (en) 2019-12-31
JP2019088305A (ja) 2019-06-13
MX2020012356A (es) 2021-01-29
IL270861B (en) 2021-05-31
EA201691532A1 (ru) 2017-01-30
BR112016017606A2 (pt) 2017-10-10
US10655156B2 (en) 2020-05-19
AU2014380174B2 (en) 2018-08-30
JP7034236B2 (ja) 2022-03-11
US10227627B2 (en) 2019-03-12
CL2016001900A1 (es) 2017-05-12
JP6474420B2 (ja) 2019-02-27
IL282517B (en) 2022-07-01
CA2938079A1 (en) 2015-08-06
CN106170554A (zh) 2016-11-30
JP6797950B2 (ja) 2020-12-09
US20200277642A1 (en) 2020-09-03
JP2017505620A (ja) 2017-02-23
EA037565B1 (ru) 2021-04-14
AU2018264100B2 (en) 2020-07-23
AU2018264100A1 (en) 2018-12-06
JP2022068365A (ja) 2022-05-09
EA202190244A2 (ru) 2021-05-31
JP2021040638A (ja) 2021-03-18
US20190010532A1 (en) 2019-01-10
US10907186B2 (en) 2021-02-02
IL246914A0 (en) 2016-09-29
CN113774084B (zh) 2024-04-16
IL282517A (en) 2021-06-30
KR102301034B1 (ko) 2021-09-13
KR102519540B1 (ko) 2023-04-10
KR20160111943A (ko) 2016-09-27
CA2938079C (en) 2022-10-11
AU2020239681B2 (en) 2022-06-23
AU2020239681A1 (en) 2020-10-15
US20190153496A1 (en) 2019-05-23
EP3099806A1 (en) 2016-12-07
KR20210114549A (ko) 2021-09-23
CN113774084A (zh) 2021-12-10
IL270861A (en) 2020-01-30
EA202190244A3 (ru) 2021-08-31
WO2015116315A1 (en) 2015-08-06
SG10201912081RA (en) 2020-02-27
CN106170554B (zh) 2021-08-17

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