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MX344363B - Sistemas y métodos para reacciones biológicas paralelas reutilizables automatizadas. - Google Patents

Sistemas y métodos para reacciones biológicas paralelas reutilizables automatizadas.

Info

Publication number
MX344363B
MX344363B MX2013003860A MX2013003860A MX344363B MX 344363 B MX344363 B MX 344363B MX 2013003860 A MX2013003860 A MX 2013003860A MX 2013003860 A MX2013003860 A MX 2013003860A MX 344363 B MX344363 B MX 344363B
Authority
MX
Mexico
Prior art keywords
local
biological material
products
electric field
change
Prior art date
Application number
MX2013003860A
Other languages
English (en)
Other versions
MX2013003860A (es
Inventor
Esfandyarpour Hesaam
Original Assignee
Genapsys Inc *
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genapsys Inc * filed Critical Genapsys Inc *
Publication of MX2013003860A publication Critical patent/MX2013003860A/es
Publication of MX344363B publication Critical patent/MX344363B/es

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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  • Chemical & Material Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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Abstract

La presente invención se refiere a un método que comprende mantener magnéticamente una perla que tenga material biológico (por ejemplo, ácido nucleico, que puede estar en forma de fragmentos de ADN o ADN amplificado) en una ubicación específica de un sustrato, y aplicar un campo eléctrico local a la perla para aislar el material biológico o productos o subproductos de reacción del material biológico. Por ejemplo, la perla se aísla de otras perlas con material biológico asociado. El campo eléctrico en diversas realizaciones concentra reactivos para una reacción de amplificación o secuenciación y/o concentra y aísla subproductos de reacción detectables. Por ejemplo, mediante el aislamiento de los ácidos nucleicos alrededor de las perlas individuales, el campo eléctrico puede permitir una amplificación clonal como una alternativa a la PCR de emulsión. En otras realizaciones, el campo eléctrico aísla un nanosensor próximo a la perla, para facilitar la detección de al menos uno de un cambio de pH local, cambio de conductividad local, cambio de concentración de carga local y calor local. Las perlas pueden estar atrapadas en forma de un arreglo de regiones de campo magnético localizado.
MX2013003860A 2010-10-04 2011-10-04 Sistemas y métodos para reacciones biológicas paralelas reutilizables automatizadas. MX344363B (es)

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