ES2608313T3 - Identificación de resistencia antibiótica en microorganismos - Google Patents
Identificación de resistencia antibiótica en microorganismos Download PDFInfo
- Publication number
- ES2608313T3 ES2608313T3 ES11158836T ES11158836T ES2608313T3 ES 2608313 T3 ES2608313 T3 ES 2608313T3 ES 11158836 T ES11158836 T ES 11158836T ES 11158836 T ES11158836 T ES 11158836T ES 2608313 T3 ES2608313 T3 ES 2608313T3
- Authority
- ES
- Spain
- Prior art keywords
- bacterial cell
- nucleic acid
- individual bacterial
- marker
- selectively
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000003115 biocidal effect Effects 0.000 title abstract 4
- 244000005700 microbiome Species 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 abstract 15
- 108020004707 nucleic acids Proteins 0.000 abstract 9
- 102000039446 nucleic acids Human genes 0.000 abstract 9
- 150000007523 nucleic acids Chemical class 0.000 abstract 9
- 239000012634 fragment Substances 0.000 abstract 5
- 239000003550 marker Substances 0.000 abstract 5
- LHNIIDJCEODSHA-OQRUQETBSA-N (6r,7r)-3-[(e)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid Chemical compound N([C@H]1[C@H]2SCC(=C(N2C1=O)C(=O)O)\C=C\C=1C(=CC(=CC=1)[N+]([O-])=O)[N+]([O-])=O)C(=O)CC1=CC=CS1 LHNIIDJCEODSHA-OQRUQETBSA-N 0.000 abstract 4
- 108091023037 Aptamer Proteins 0.000 abstract 4
- 108010025905 Cystine-Knot Miniproteins Proteins 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 238000001514 detection method Methods 0.000 abstract 2
- 238000011065 in-situ storage Methods 0.000 abstract 2
- 239000000523 sample Substances 0.000 abstract 2
- 108090000204 Dipeptidase 1 Proteins 0.000 abstract 1
- 102000006635 beta-lactamase Human genes 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/136—Screening for pharmacological compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
- G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/44—Multiple drug resistance
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método para la identificación simultánea de una célula bacteriana individual y la detección de una resistencia antibiótica en dicha célula bacteriana individual en una muestra biológica, que comprende las etapas: (a) poner en contacto dicha célula bacteriana individual con un primer ácido nucleico capaz de hibridarse selectivamente con un ácido nucleico en dicha célula bacteriana en condiciones in situ, en donde el primer ácido nucleico se hibrida selectivamente con el ácido nucleico, y en el que el primer ácido nucleico se marca con un primer marcador, (b) identificar la célula bacteriana por la detección de la presencia del primer marcador en dicha célula bacteriana individual, (c) poner en contacto dicha misma célula bacteriana individual con al menos una sonda para la detección de una resistencia antibiótica en la célula bacteriana en las mismas dichas condiciones in situ, en el que dicha al menos una sonda se selecciona de (i) anticuerpos y fragmentos de los mismos, siendo capaces los anticuerpos y fragmentos de los mismos de unirse selectivamente a un factor de resistencia, y en el que el anticuerpo y los fragmentos de los mismos se marcan con un segundo marcador, en donde dicha célula bacteriana individual se pone en contacto con el anticuerpo y/o dicho fragmento del mismo en condiciones en donde el anticuerpo y/o el fragmento del mismo se une selectivamente al factor de resistencia, (ii) segundos ácidos nucleicos capaces de hibridarse con un ARN que codifica para un factor de resistencia, en el que el segundo ácido nucleico se marca con un tercer marcador, en donde dicha célula bacteriana individual se pone en contacto con el segundo ácido nucleico bajo condiciones en las que el segundo ácido nucleico se hibrida selectivamente con el ARN, (iii) knottins, proteínas cistina-nudo o/y aptámeros capaces de unirse selectivamente a un factor de resistencia, y en el que los knottins, las proteínas cistina-nudo o/y los aptámeros se marcan con un cuarto marcador, en donde dicha célula bacteriana individual se pone en contacto con el knottin, proteína de cistina-nudo y/o aptámero en condiciones en las que el knottin, la proteína de cistina- nudo y/o el aptámero se unen selectivamente al factor de resistencia, y (iv) nitrocefina, en donde dicha célula bacteriana individual se pone en contacto con dicha nitrocefina en condiciones en las que beta-lactamasa modifica a dicha nitrocefina, y (d) determinar la resistencia a los antibióticos de la célula bacteriana por la detección de la presencia de uno o más marcadores, el segundo marcador, el tercer marcador, el cuarto marcador, o/y la nitrocefina modificada en la célula bacteriana individual, en donde las etapas (b) y (d) se realizan simultáneamente en dicha célula bacteriana individual.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11158836.4A EP2500435B1 (en) | 2011-03-18 | 2011-03-18 | Identification of antibiotic resistance in micro organisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2608313T3 true ES2608313T3 (es) | 2017-04-07 |
Family
ID=45952473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES11158836T Active ES2608313T3 (es) | 2011-03-18 | 2011-03-18 | Identificación de resistencia antibiótica en microorganismos |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20140295417A1 (es) |
| EP (1) | EP2500435B1 (es) |
| ES (1) | ES2608313T3 (es) |
| WO (1) | WO2012126882A1 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230169418A (ko) | 2013-03-13 | 2023-12-15 | 메소 스케일 테크놀러지즈, 엘엘시 | 개선된 분석 방법 |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| CA2916352C (en) | 2013-07-03 | 2020-09-08 | Qvella Corporation | Methods of targeted antibiotic susceptibility testing |
| CN103645273B (zh) * | 2013-12-12 | 2015-12-30 | 宁波诺威医疗器械有限公司 | β-内酰胺类抗生素耐药性的检测方法、系统及其应用 |
| EP3143401A4 (en) | 2014-05-15 | 2017-10-11 | Meso Scale Technologies, LLC | Improved assay methods |
| CN105695598A (zh) * | 2016-03-25 | 2016-06-22 | 苏州达麦迪生物医学科技有限公司 | 用于耐甲氧西林金黄色葡萄球菌检测的分子信标探针、试剂盒及方法 |
| US11268960B2 (en) | 2018-01-10 | 2022-03-08 | SeLux Diagnostics, Inc. | Assays for improving automated antimicrobial susceptibility testing accuracy |
| EP3556862A1 (en) * | 2018-04-17 | 2019-10-23 | Universitat Autonoma de Barcelona | Rapid detection of antibiotic resistance by bacterial ribosome immunoprecipitation |
| CN109486739B (zh) * | 2018-11-23 | 2022-02-01 | 上海海洋大学 | 一种诱导副溶血性弧菌产生左氧氟沙星耐药性的方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040242521A1 (en) * | 1999-10-25 | 2004-12-02 | Board Of Regents, The University Of Texas System | Thio-siRNA aptamers |
| JP2005518553A (ja) * | 2001-09-06 | 2005-06-23 | ジェノミック プロファイリング システムズ インコーポレイティッド | 細胞およびウイルスの迅速かつ高感度な検出方法 |
| US20030186299A1 (en) * | 2002-02-27 | 2003-10-02 | Cogburn Larry A. | Molecular markers for identification of fat and lean phenotypes in chickens |
| WO2004097417A1 (en) * | 2003-05-02 | 2004-11-11 | Canon Kabushiki Kaisha | Structured construct and producing method therefor |
| WO2008002600A2 (en) * | 2006-06-26 | 2008-01-03 | The Regents Of The University Of California | Biosynthesis of salinosporamide a and analogs and methods thereof |
| EP2097541B1 (en) | 2006-10-10 | 2012-12-12 | miacom Diagnostics GmbH | Nucleic acid beacons for fluorescent in-situ hybridisation and chip technology |
| CN101965407B (zh) * | 2008-02-01 | 2014-11-26 | 米阿科姆诊断有限公司 | 使用经标记的抗生素鉴定抗生素耐受性 |
| WO2009137677A2 (en) * | 2008-05-07 | 2009-11-12 | Innovative Biosensors, Inc. | Reagents, methods, and systems for detecting methicillin-resistant staphylococcus |
| US20110269130A1 (en) * | 2008-10-24 | 2011-11-03 | Song Shi | Antibiotice Susceptibility Profiling Methods |
| AU2010248809B2 (en) * | 2009-05-15 | 2015-07-09 | Biomerieux, Inc. | System and methods for rapid identification and/or characterization of a microbial agent in a sample |
| US9279160B2 (en) * | 2009-06-22 | 2016-03-08 | Statens Serum Institut | DNA-based methods for clone-specific identification of staphylococcus aureus |
-
2011
- 2011-03-18 ES ES11158836T patent/ES2608313T3/es active Active
- 2011-03-18 EP EP11158836.4A patent/EP2500435B1/en not_active Not-in-force
-
2012
- 2012-03-19 WO PCT/EP2012/054803 patent/WO2012126882A1/en active Application Filing
- 2012-03-19 US US14/005,946 patent/US20140295417A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20140295417A1 (en) | 2014-10-02 |
| EP2500435B1 (en) | 2016-09-21 |
| EP2500435A1 (en) | 2012-09-19 |
| WO2012126882A1 (en) | 2012-09-27 |
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