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MX2013008103A - Purificacion de inhibidor de proteasa alfa1 derivado de cultivo celular. - Google Patents

Purificacion de inhibidor de proteasa alfa1 derivado de cultivo celular.

Info

Publication number
MX2013008103A
MX2013008103A MX2013008103A MX2013008103A MX2013008103A MX 2013008103 A MX2013008103 A MX 2013008103A MX 2013008103 A MX2013008103 A MX 2013008103A MX 2013008103 A MX2013008103 A MX 2013008103A MX 2013008103 A MX2013008103 A MX 2013008103A
Authority
MX
Mexico
Prior art keywords
cell culture
protease inhibitor
culture derived
purification
derived alpha1
Prior art date
Application number
MX2013008103A
Other languages
English (en)
Other versions
MX341733B (es
Inventor
David Ownby
Charles Miller
Senthil Ranganathan
Tonny Dessources
Zimmerman Tom
Hunt Jennifer
Original Assignee
Grifols Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48748028&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2013008103(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Grifols Sa filed Critical Grifols Sa
Publication of MX2013008103A publication Critical patent/MX2013008103A/es
Publication of MX341733B publication Critical patent/MX341733B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4703—Inhibitors; Suppressors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/99—Enzyme inactivation by chemical treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

En el presente documento se describen métodos para purificar un inhibidor de proteasa alfa1 derivado de cultivo celular recombinante y eliminar una especie coloreada que purifica conjuntamente con la proteína recA1PI. También se describen métodos para reducir el hierro en el inhibidor de proteasa alfa1 derivado de cultivo celular.
MX2013008103A 2012-07-25 2013-07-11 Purificacion de inhibidor de proteasa alfa1 derivado de cultivo celular. MX341733B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261675560P 2012-07-25 2012-07-25

Publications (2)

Publication Number Publication Date
MX2013008103A true MX2013008103A (es) 2014-03-27
MX341733B MX341733B (es) 2016-08-31

Family

ID=48748028

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013008103A MX341733B (es) 2012-07-25 2013-07-11 Purificacion de inhibidor de proteasa alfa1 derivado de cultivo celular.

Country Status (23)

Country Link
US (2) US9353165B2 (es)
EP (1) EP2690110B1 (es)
JP (1) JP6096613B2 (es)
KR (1) KR101877352B1 (es)
CN (1) CN103570828B (es)
AR (1) AR091712A1 (es)
AU (1) AU2013203772B2 (es)
BR (1) BR102013016279B1 (es)
CA (1) CA2821384C (es)
CL (1) CL2013002024A1 (es)
ES (1) ES2628914T3 (es)
HU (1) HUE033582T2 (es)
IL (1) IL227409A (es)
MX (1) MX341733B (es)
MY (1) MY161395A (es)
NZ (1) NZ613080A (es)
PL (1) PL2690110T3 (es)
PT (1) PT2690110T (es)
RU (1) RU2617960C2 (es)
SG (1) SG196723A1 (es)
TW (1) TWI593702B (es)
UY (1) UY34915A (es)
ZA (1) ZA201305247B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016106291A1 (en) * 2014-12-22 2016-06-30 Alexion Pharmaceuticals, Inc. Methods of purifying recombinant proteins
MX2020004151A (es) 2017-10-30 2020-08-13 Takeda Pharmaceuticals Co Detergentes compatibles con el medio ambiente para la inactivacion de virus envueltos en lipidos.
BR112022016234A2 (pt) * 2020-02-25 2022-10-11 Grifols Worldwide Operations Ltd Método para obtenção de inibidor de proteinase alfa-1
KR102284800B1 (ko) * 2020-12-30 2021-08-03 주식회사 하플사이언스 재조합 세포외 기질 단백질의 생산을 위한 동물세포 배양용 배지 조성물 및 이를 이용한 방법

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60186290A (ja) * 1983-08-10 1985-09-21 チモ−ジエネテイツクス　インコ−ポレ−テツド アルフア−１−アンチトリプシンを細菌に表現させる方法
US4931373A (en) 1984-05-25 1990-06-05 Zymogenetics, Inc. Stable DNA constructs for expression of α-1 antitrypsin
US5134119A (en) 1990-10-16 1992-07-28 Lezdey John Treatment of inflammation using 358 substituted alpha-antitrypsin
US6284874B1 (en) 1994-06-17 2001-09-04 Alpha Therapeutic Corporation Process for separating α1-proteinase inhibitor from cohn fraction IV1 and IV4 paste
CA2170610C (en) * 1994-06-27 2007-05-22 Ioannis Stavridis Removal of noxious oxidants and carcinogenic volatile nitrosocompounds from cigarette smoke using biological substances
US5616693A (en) 1996-07-01 1997-04-01 Alpha Therapeutic Corporation Process for seperating alpha-1-proteinase inhibitor from COHN IV1 +1V4 paste
US6093804A (en) 1998-09-24 2000-07-25 American National Red Cross Method for purification of alpha-1 proteinase inhibitor
US6462180B1 (en) 1999-11-24 2002-10-08 Bayer Corporation Method of preparing α-1 proteinase inhibitor
CA2432641A1 (en) 2000-12-14 2002-06-20 Bayer Corporation Method of preparing alpha-1 proteinase inhibitor
EP1366062B1 (en) * 2001-02-23 2011-07-27 Immunex Corporation Efficient recovery of correctly refolded proteins
US7777006B2 (en) * 2002-12-31 2010-08-17 Csl Behring L.L.C. Method for purification of alpha-1-antitrypsin
WO2005014655A2 (en) * 2003-08-08 2005-02-17 Fresenius Kabi Deutschland Gmbh Conjugates of hydroxyalkyl starch and a protein
WO2005014648A1 (en) 2003-08-12 2005-02-17 Octapharma Ag Process for preparing an alpha-1-antitrypsin solution
EP1664123B2 (en) 2003-09-22 2011-11-30 Kamada Ltd. Large scale preparation of alpha-1 proteinase inhibitor and use thereof
EP1685160A1 (en) 2003-11-10 2006-08-02 Arriva-Prometic Inc. Dry recombinant human alpha 1-antitrypsin formulation
US7807435B2 (en) * 2005-08-11 2010-10-05 Baxter International Inc. Method for the purification of alpha-1 proteinase inhibitor (a1PI)
US20070218535A1 (en) 2005-11-28 2007-09-20 Xinli Lin Methods for production of recombinant alpha1-antitrypsin
GB0524432D0 (en) 2005-11-30 2006-01-11 Nhs Blood & Transplant Method
ES2395667T3 (es) 2006-03-30 2013-02-14 Baxter International Inc. Proceso para la purificación de alfa-1-antitripsina recombinante que implica un paso de cromatografía de intercambio aniónico
NZ582170A (en) * 2007-08-17 2012-02-24 Csl Behring Gmbh Methods for purification of alpha-1-antitrypsin and apolipoprotein a-i
CN101835799A (zh) * 2007-08-24 2010-09-15 麦莱克萨有限公司 超敏反应调节剂
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AU2012262428C1 (en) * 2011-05-27 2018-10-11 Takeda Pharmaceutical Company Limited Therapeutic proteins with increased half-life and methods of preparing same

Also Published As

Publication number Publication date
US9957315B2 (en) 2018-05-01
KR101877352B1 (ko) 2018-07-11
ES2628914T3 (es) 2017-08-04
PL2690110T3 (pl) 2017-09-29
MY161395A (en) 2017-04-14
CA2821384A1 (en) 2014-01-25
PT2690110T (pt) 2017-06-27
CL2013002024A1 (es) 2014-08-08
TWI593702B (zh) 2017-08-01
IL227409A0 (en) 2013-12-31
CN103570828B (zh) 2017-12-15
SG196723A1 (en) 2014-02-13
US20160304583A1 (en) 2016-10-20
MX341733B (es) 2016-08-31
BR102013016279A2 (pt) 2015-07-14
US20140031532A1 (en) 2014-01-30
HUE033582T2 (hu) 2017-12-28
JP6096613B2 (ja) 2017-03-15
NZ613080A (en) 2015-01-30
BR102013016279B1 (pt) 2022-11-08
AU2013203772B2 (en) 2015-01-22
CA2821384C (en) 2019-03-26
AU2013203772A1 (en) 2014-02-13
ZA201305247B (en) 2017-08-30
AR091712A1 (es) 2015-02-25
RU2013132132A (ru) 2015-01-20
IL227409A (en) 2017-02-28
US9353165B2 (en) 2016-05-31
EP2690110A1 (en) 2014-01-29
CN103570828A (zh) 2014-02-12
EP2690110B1 (en) 2017-05-03
RU2617960C2 (ru) 2017-04-28
UY34915A (es) 2014-02-28
TW201406775A (zh) 2014-02-16
KR20140013925A (ko) 2014-02-05
JP2014024842A (ja) 2014-02-06

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