[go: up one dir, main page]

KR880010118A - 배지의 당분농도 제어에 의한 외래유전자 발현 제어 방법 및 그에 따른 외래유전자 산물의 생산방법 - Google Patents

배지의 당분농도 제어에 의한 외래유전자 발현 제어 방법 및 그에 따른 외래유전자 산물의 생산방법 Download PDF

Info

Publication number
KR880010118A
KR880010118A KR1019880001741A KR880001741A KR880010118A KR 880010118 A KR880010118 A KR 880010118A KR 1019880001741 A KR1019880001741 A KR 1019880001741A KR 880001741 A KR880001741 A KR 880001741A KR 880010118 A KR880010118 A KR 880010118A
Authority
KR
South Korea
Prior art keywords
coli
foreign gene
expression
plasmid
controlling
Prior art date
Application number
KR1019880001741A
Other languages
English (en)
Other versions
KR910001811B1 (ko
Inventor
노부히로 후꾸하라
세쯔오 요시노
사도리 소네
요시유끼 나까지마
노부요시 마끼구찌
Original Assignee
미지마 마사요시
미쯔이도오아쯔가가꾸 가부시기가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP62034396A external-priority patent/JPS63202385A/ja
Priority claimed from JP62152358A external-priority patent/JPH0817703B2/ja
Application filed by 미지마 마사요시, 미쯔이도오아쯔가가꾸 가부시기가이샤 filed Critical 미지마 마사요시
Publication of KR880010118A publication Critical patent/KR880010118A/ko
Application granted granted Critical
Publication of KR910001811B1 publication Critical patent/KR910001811B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

내용 없음

Description

배지의 당분농도 제어에 의한 외래유전자 발현 제어방법 및 그에 따른 외래유전자 산물의 생산방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (12)

  1. 외래 유전자의 발현을 가능케 하기 위해 상기 외래 유전자를 발현벡터에 삽입시켜 구축되는 조환체플라스미드를 도입한 대장균의 균체에서 외래 유전자 발현을 제어하는 방법에 있어서, 상기 외래 유전자 발현 제어를 위해 대장균을 배양하기 위한 배지중의 탄소원인 당분농도를 0.3% 이상으로 유지하는 공정을 포함하는 것을 특징으로 하는 외래 유전자 발현 제어방법.
  2. 제1항에 있어서, 상기 외래 유전자가 L-페닐알라닌 암모니아리아제를 코우딩하는 구조 유전자임을 특징으로 하는 외래 유전자 발현 제어방법.
  3. 제2항에 있어서, 상기 L-페닐알라닌 암모니아리아제를 코우딩하는 구조 유전자임을 특징으로 하는 외래 유전자 발현 제어방법.
  4. 제1항에 있어서, 상기 대장균이 조환체 플라스미드 pSYPL-3을 도입한 대장균, 수탁번호 FERMP-9024(MT-10424)임을 특징으로 하는 외래 유전자 발현 제어방법.
  5. 제1항에 있어서, 상기 대장균이 조환체 플라스미드 pYtrp 6을 도입한 대장균, 수탁번호 FERMP-8876(MT-10414)임을 특징으로 하는 외래 유전자 발현 제어방법.
  6. 제1항에 있어서, 상기 대장균이 조환체 플라스미드 pSW 115을 도입한 대장균, 수탁번호 FERMP-9023(MT-10423)임을 특징으로 하는 외래 유전자 발현 제어방법.
  7. 소망의 외래 유전자의 발현을 가능케 하기 위해 상기 외래 유전자를 발현 벡터에 삽입시켜 구축되는 조환체 플라스미드를 도입한 대장균을 배양하여 소망의 외래 유전자 산물을 생산하는 과정에 있어서, (a) 배지중의 탄소원으로서 당분 농도가 0.3%이상으로 유지되는, 상기 대장균을 배양하기 위한 제1배양과정과 (b) 배지중의 상기 당분 농도가 0.3% 미만으로 유지되는, 제1배양 과정에서 증식된 상기 대장균을 배양하기 위한 제2배양과정의 단계들로 이루어지는 것을 특징으로 하는 외래 유전자 산물의 생산방법.
  8. 제7항에 있어서, 상기 외래 유전자 산물이 L-페닐알라닌 암모니아리아제임을 특징으로 하는 외래 유전자 산물의 생산방법.
  9. 제8항에 있어서, 상기 L-페닐알리닌 암모니아리아제가 제3항에 지시된 아미노산 배열을 가짐을 특징으로 하는 외래 유전자 산물의 생산방법.
  10. 제7항에 있어서, 상기 대장균이 조환체 플라스미드 pSYPL-3을 도입한 대장균, 수탁번호 FERMP-9024(MT-210424)임을 특징으로 하는 외래 유전자 산물의 생산방법.
  11. 제7항에 있어서, 상기 대장균이 조환체 플라스미드 pYtrp 6을 도입한 대장균, 수탁번호 FERMP-8876(MT-10414)임을 특징으로 하는 외래 유전자 산물의 생산방법.
  12. 제7항에 있어서, 상기 대장균이 조환체 플라스미드 pSW 115을 도입한 대장균, 수탁번호 FERMP-9023(MT-10423)임을 특징으로 하는 외래 유전자 산물의 생산방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880001741A 1987-02-19 1988-02-19 배지의 당분농도제어에 의한 외래유전자 발현제어방법 및 그에 따른 외래유전자 산물의 생산방법 KR910001811B1 (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP34396 1987-02-19
JP62034396A JPS63202385A (ja) 1987-02-19 1987-02-19 外来遺伝子の発現制御方法及び該方法を用いた外来遺伝子産物の生産方法
JP62-34396 1987-02-19
JP62-152358 1987-06-18
JP152358 1987-06-18
JP62152358A JPH0817703B2 (ja) 1987-06-18 1987-06-18 外来遺伝子産物の生産方法

Publications (2)

Publication Number Publication Date
KR880010118A true KR880010118A (ko) 1988-10-07
KR910001811B1 KR910001811B1 (ko) 1991-03-26

Family

ID=26373201

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019880001741A KR910001811B1 (ko) 1987-02-19 1988-02-19 배지의 당분농도제어에 의한 외래유전자 발현제어방법 및 그에 따른 외래유전자 산물의 생산방법

Country Status (7)

Country Link
EP (1) EP0279664B1 (ko)
KR (1) KR910001811B1 (ko)
CA (1) CA1319630C (ko)
DE (1) DE3882247T2 (ko)
DK (1) DK88588A (ko)
ES (1) ES2058250T3 (ko)
MX (1) MX168450B (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4946790A (en) * 1987-02-06 1990-08-07 Mitsui Toatsu Chemicals, Inc. Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same
US5279951A (en) * 1989-05-08 1994-01-18 Research Association For Utilization Of Light Oil Cultivation of transformed microorganisms
DE3925550A1 (de) * 1989-08-02 1991-02-07 Behringwerke Ag Optimierte fermentationsverfahren zur herstellung von fremdproteinen in e.coli
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60221091A (ja) * 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
GB8621626D0 (en) * 1986-09-08 1986-10-15 Health Lab Service Board Phenylalanine ammonia lyase
JP2507345B2 (ja) * 1986-09-16 1996-06-12 三井東圧化学株式会社 Ｌ―フェニルアラニン―アンモニアリ・ア―ゼ構造遺伝子

Also Published As

Publication number Publication date
EP0279664B1 (en) 1993-07-14
DE3882247D1 (de) 1993-08-19
EP0279664A3 (en) 1989-06-14
ES2058250T3 (es) 1994-11-01
EP0279664A2 (en) 1988-08-24
DK88588A (da) 1988-08-20
DE3882247T2 (de) 1993-10-28
KR910001811B1 (ko) 1991-03-26
DK88588D0 (da) 1988-02-19
CA1319630C (en) 1993-06-29
MX168450B (es) 1993-05-25

Similar Documents

Publication Publication Date Title
KR930018036A (ko) 발효에 의한 아미노산 제조방법
KR960705947A (ko) 물지 제조법(Process for producing substance)
EP1095158A4 (en) METHOD AND MATERIALS FOR THE PRODUCTION OF GLUCOSAMINE
HUP9702361A2 (hu) Eljárás L-lizin előállítására
DE69330518D1 (de) Dna, die aspartokinase iii-mutanten kodiert und ihre verwendung zur herstellung von l-threonin durch fermentation
TW200504213A (en) Method of preparing amino acids by fermentation with the constructed amino acid producing bacteria
KR850007983A (ko) 히알우론산 생산의 발효법
DE88166T1 (de) Verfahren zum exprimieren eines gens.
JPS55131397A (en) Preparation of l-threonine by fermentation
ATE44047T1 (de) Verfahren zur herstellung von interferonen.
NO964754D0 (no) DNA-kjede anvendelig i ökning av karotenoid utbytte
JPS55156591A (en) Method for stabilizing property of bacteria containing plasmid
KR880010118A (ko) 배지의 당분농도 제어에 의한 외래유전자 발현 제어 방법 및 그에 따른 외래유전자 산물의 생산방법
Koesnandar et al. Production of extracellular 5-aminolevulinic acid by Clostridium thermoaceticum grown in minimal medium
DE69620820D1 (de) Verfahren zur Herstellung von L-Isoleucin durch Fermentation und L-Homoserine als einzige Stickstoffquelle
KR890008312A (ko) 조절된 중식속도에서의 발효
KR880004088A (ko) L-페닐알라닌 암모니아리아제의 아미노산배열, l-페닐알라닌 암모니아리아제의 구조유전자, 이것을 함유하는 신규의 dna배열, 이것을 함유하는 신규의 조환형 dna 플라스미드, 이것에 의해 형성되는 형질전환체 및 이것을 사용한 l-페닐알라닌의 제조방법
GB1444072A (en) Microbiological production of proteinaceous material
KR950700329A (ko) 신규한 세포 표층 단백질(Novel cell cortex protein)
JPWO2004053111A1 (ja) 外来遺伝子高発現大腸菌株の選択方法、その方法により選択される大腸菌変異株及びそれを用いる酵素及び化合物の製造方法
US6716617B1 (en) Fermentation method with continuous mass cultivation of ciliates (protozoa) for producing biogenous valuable substances
ATE451469T1 (de) In vivo-verfahren zur herstellung von proteinen, die nicht-konventionelle aminosäuren enthalten
ES8506094A1 (es) Un procedimiento para producir l-isoleucina
KR970021294A (ko) 유기산 생성이 억제된 대장균 변이주
KR940021729A (ko) 효모로부터 재조합 히루딘을 생산하는 방법

Legal Events

Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19880219

A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 19880525

Comment text: Request for Examination of Application

Patent event code: PA02011R01I

Patent event date: 19880219

Comment text: Patent Application

PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 19900529

Patent event code: PE09021S01D

G160 Decision to publish patent application
PG1605 Publication of application before grant of patent

Comment text: Decision on Publication of Application

Patent event code: PG16051S01I

Patent event date: 19910227

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 19910625

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 19910701

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 19910701

End annual number: 3

Start annual number: 1

PR1001 Payment of annual fee

Payment date: 19940315

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 19950323

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 19960322

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 19970317

Start annual number: 7

End annual number: 7

PR1001 Payment of annual fee

Payment date: 19980317

Start annual number: 8

End annual number: 8

PR1001 Payment of annual fee

Payment date: 19990312

Start annual number: 9

End annual number: 9

PR1001 Payment of annual fee

Payment date: 20000317

Start annual number: 10

End annual number: 10

PR1001 Payment of annual fee

Payment date: 20010321

Start annual number: 11

End annual number: 11

PR1001 Payment of annual fee

Payment date: 20020320

Start annual number: 12

End annual number: 12

PR1001 Payment of annual fee

Payment date: 20030311

Start annual number: 13

End annual number: 13

PR1001 Payment of annual fee

Payment date: 20040310

Start annual number: 14

End annual number: 14

PR1001 Payment of annual fee

Payment date: 20050309

Start annual number: 15

End annual number: 15

FPAY Annual fee payment

Payment date: 20060313

Year of fee payment: 16

PR1001 Payment of annual fee

Payment date: 20060313

Start annual number: 16

End annual number: 16

LAPS Lapse due to unpaid annual fee
PC1903 Unpaid annual fee