JPH04188041A - Specimen measuring apparatus - Google Patents

Abstract

PURPOSE:To provide an apparatus characterized by low power consumption and long life by turning OFF the light source of a beam which is not used in response to a measuring mode. CONSTITUTION:A plurality of modes are changed in response to applications, and a laser light source which is not used is turned OFF. Such a function is provided. In details, the mode can be changed into the first mode wherein two laser lights 2 and 3 are emitted at the same time and the measurement in time series is performed and the second mode wherein either of the laser lights is emitted and the other is not emitted. At this time, a control means 162 independently controls the ON/OFF of the emission from each laser light source in response to the measuring conditions set with an input setting means 160. The laser light source not in use is set in the sleep mode, or the power supply for the laser light source is cut off. In this way, the low power consumption of the system and the elongation of the life of the laser are achieved.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to the field of specimen measurement, in which specimens are analyzed by irradiating each specimen with light and performing optical measurements.

[Prior Art] Flow cytometers have been known as an example of sample testing devices, and are widely used in the biological and medical fields.

A typical configuration of this flow cytometer is shown in FIG. A sample liquid such as blood is pretreated by staining with a fluorescent reagent or the like and adjusted to an appropriate reaction time and dilution concentration. Then, put this into the sample liquid container 115. Further, a sheath liquid such as distilled water or physiological saline is placed in the sheath liquid container 114. The sample liquid container 115 and the sheath liquid container 114 are each pressurized by a pressurizing mechanism (not shown). Then, according to the sheath flow principle, the sample liquid is wrapped in the sheath liquid in the flow cell 104 and converged into a thin flow, and the flow cell 10
It passes through approximately the center of the flow section in 4.

At this time, individual test particles (cells,
(microorganisms, carrier particles, etc.), that is, the specimen is separated and sequentially flows one particle or one lump at a time. A laser beam emitted from a laser light source 101 is converged into an arbitrary shape by a pair of cylindrical lenses 102 and 103 whose generatrix directions are in the direction of the flow section and perpendicular to the direction of the flow section, respectively, and irradiate the flow of the particles to be detected. be done. Generally, it is preferable that the shape of the light beam irradiated onto the test particles is an ellipse having a major axis in a direction perpendicular to the flow. This is to ensure that the light beam is irradiated with uniform intensity onto the test particles even if the flow position of each test particle varies slightly in the fluid.

When a light beam is irradiated onto a particle to be examined, scattered light is generated. Among the scattered lights, the forward scattered lights emitted in the forward direction of the optical path are photometered by a condenser lens 105 and a photodetector 106. In order to prevent the irradiated light beam from directly entering the photodetector 106, a small light-absorbing stopper 100 is provided in the optical path in front of the condensing lens 105, so that the direct light from the irradiation light source, And the transmitted light that has passed through the test particles is removed. This makes it possible to photometer only the scattered light from the test particles.

Of the scattered light, light emitted in the lateral direction perpendicular to the laser optical axis and the flow of the particles to be detected is transmitted through the condenser lens 10.
The light is focused at 7. The focused light is reflected by the dichroic mirror 108, and the wavelength of the scattered light, that is, the wavelength of the laser light (
The side scattered light is measured by the photodetector 111 through a bandpass filter 121 that selectively transmits light (488 nm in the case of an Ar+ laser). In addition, when the test particles are fluorescently dyed, in order to photometer the fluorescence of multiple colors generated together with the scattered light, among the fluorescence that is collected by the condenser lens 107 and passed through the dichroic mirror 108, the dichroic mirror 109 , a bandpass filter 122 for green fluorescence wavelength (around 530 nm), and a photodetector 112, and a total reflection mirror 110 detects the green fluorescence.
, a bandpass filter 123 for red fluorescence wavelength (near 57 Onm), and a photodetector 113 detect red fluorescence. The signals of the photodetectors 106, 111, 112, and 113 are each input to an arithmetic circuit 116, and the arithmetic circuit 11
In step 6, calculations such as analysis of particle types and properties, measurement of antigen-antibody reactions, etc. are performed.

[Problems to be Solved by the Invention] However, conventionally, a dedicated photodetector has been used for each fluorescence to measure fluorescence of a plurality of colors.

Until now, it was common to have a configuration that simultaneously measured two colors, red fluorescence and green fluorescence, or three colors with yellow fluorescence added, but in recent years there has been an increasing demand for even more colors, and new fluorescence Development of drugs is also progressing.

As a result, if the number of fluorescence channels used simultaneously increases, the number of photodetectors will also increase accordingly. That is, there is a problem in that the optical arrangement becomes complicated and a large number of expensive photodetectors such as photomultipliers are required.

Therefore, in Japanese Patent Application No. 1-325001, etc., the applicant proposed a device that can obtain measurement parameters greater than the number of photodetectors by detecting multiple types of light in time series using a common photodetector. did. The present invention aims at further improvements to the proposed device.

[Means for Achieving the Object] A sample measuring device of the present invention that achieves the above object includes a setting means for setting a measurement mode, and a first irradiation light for generating and irradiating a first position where the sample moves. a first irradiation means for
a second irradiation means that generates irradiation light and irradiates it to a second position different from the first position; and first and second optical characteristics emitted from the specimen passing through the first and second positions. a light detection means for detecting light of
[Embodiment] Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

FIGS. 1 and 2 show configuration diagrams of the first embodiment. 1st
The figure is a basic configuration diagram of the embodiment, and represents the entire system including a front optical system, a fluid system, a control system, etc. In the figure, reference numeral 1 denotes a flow cell that forms a so-called sheath flow in which test particles (such as biological cells or carrier particles) in a sample liquid are separated one by one by being wrapped in a sheath liquid and flowed in sequence. The particles to be tested flow through the internal flow section from above to below the page. The fluid system for forming a sheath flow includes a sample liquid container 150 that accumulates sample liquid such as a blood sample or immune reaction liquid, a pump 152 that pressurizes the container, and an electric regulator that adjusts the flow rate of the sample liquid. 154, a sheath liquid container 151 that accumulates a sheath liquid such as physiological saline, a pump 153 that pressurizes it, an electric regulator 155 that adjusts the flow rate of the sheath liquid, and a reservoir that fluidly connects these and guides them into the flow cell l. It consists of a tube. Sample liquid container 150
The inside of the sheath liquid container 151 is pressurized by the pump 153 to push out the sheath liquid, and the flow rate adjustment by each of the regulators 154 and 155 changes the state inside the flow cell l, i.e. The flow speed, flow interval of individual particles, etc. are set.

In addition to the above-mentioned pressurizing mechanism using a pump and regulator, it is also possible to adopt a configuration using a syringe as shown in Japanese Patent Application Laid-Open No. 2-213744 and adjust the extrusion speed of the syringe. good.

Now, the front optical system will be explained next. 2゜3 is a laser light source with different wavelengths, which is commonly used in this field.
Lasers such as an r+ laser, a He-Ne laser, a dye laser, and a semiconductor laser, as well as various light sources other than lasers, can be used. As shown in Figure 5(D), the laser light source is
If more than one light source is prepared and the laser beam from each light source is selectively guided to the irradiation optical path according to the measurement conditions, a highly versatile system capable of performing even more versatile measurements can be obtained. In FIG. 5(D), 74 is a total reflection mirror, 75 is a half mirror, and 176 is an optical shutter.

Returning to FIGS. 1 and 2, 4a and 4b are condenser lenses that image the irradiation light beam onto the test area of the flow cell section,
Laser beams from laser light sources 2 and 3 are imaged at positions la and lb in the flow cell, respectively. The shape of the imaging beam is preferably an ellipse with the major axis in the direction perpendicular to the flow.

Here, the distance between both irradiation positions 1a and lb is about 100 μm, which is larger than the size of the particles to be measured.
This is sufficiently shorter than the flow interval of particles flowing sequentially. Members 5a and 5b disposed in the straight beam direction are light stoppers for blocking the laser beams from the laser light sources 2 and 3, respectively, to form a dark field optical system, and 6 is a condenser for condensing forward scattered light. Optical lens, 7 is field diaphragm, la,
The open lower a is located at the conjugate position corresponding to the position of lb.

7b is provided. 8a and 8b are photodetectors that detect forward scattered light from positions 1a and lb.

Note that it is not necessarily necessary to prepare two laser light sources to form two laser beams. For example, Figure 5 (A)
(B) A beam from a single laser source as in (C),
It may be branched into two by a Mira member. At this time, if a configuration as shown in FIGS. 5(B) and 5(C) is adopted, a plurality of beams with different wavelengths can be obtained. FIG. 5A shows an optical system in which a laser beam from a short wavelength single mode laser is generated into two beams of the same wavelength by a half mirror 71 and a total reflection mirror 72. Further, in FIG. 5(B), the laser beam is split into two beams by a half mirror 71 and a total reflection mirror 72, and each branched beam is sent to a wavelength converting member 772.7.
7b (nonlinear optical element, AO, etc.) is an optical system that generates two beams of different wavelengths by converting the wavelength. Furthermore, FIG. 5(C) shows an optical system that splits a laser beam from a multimode laser having a plurality of wavelengths into two beams of different wavelengths using a dichroic mirror 73 to produce two beams of beams of different wavelengths.

In the configuration shown in FIG. 1, laser light emitted from the laser light source 2 is focused by a condenser lens 4a and irradiated onto the test area 1a. When the test particles pass through the test region 1a, the test particles cause light scattering, and at this time, if the test particles are fluorescently dyed, fluorescence is also excited and generated together with the scattered light. A part of the generated scattered light travels in the forward direction of the optical path and becomes forward scattered light. This forward scattered light passes through the condensing lens 6 and the aperture 7a of the field stop 7, and then passes through the photodetector 8a.
The intensity is detected and a first forward scattering signal is obtained. Similarly, the laser light emitted from the laser light source 3 is focused by the condenser lens 4b and irradiated onto the test area 1b.

When the test particles pass through the test region 1b, the forward scattered light passes through the opening 7b, and the intensity is detected by the photodetector 8b to obtain a second forward scattered signal.

Reference numeral 160 denotes an input setting means for inputting and setting various measurement conditions such as various modes of the system, flow rate and passing interval of particles to be detected, type of fluorescent agent to be used, and measurement items. 161
is a storage arithmetic circuit, and the outputs of photodetectors 8a and 8b and the outputs of photodetectors 25 and 23 of the side optical system, which will be described later, are connected to a gain-switchable amplification amplifier, a peak hold circuit, an integration circuit, and /D converter etc., and digital data of peak values and integral values is stored in a storage means. Particle analysis calculations are later performed based on this data, and the results are output to output means such as a CRT or printer.

As for the analysis method, statistical processing using histograms and cytograms is common, but detailed explanation will be omitted here. The storage/calculation means 161 also has a function of calculating the flow velocity from the detection output timing of the two forward scattered lights, canceling the acquisition of erroneous measurement data, etc. 16
Reference numeral 2 denotes a control means that performs comprehensive control of various operating means of the system. Specifically, pump 1
52, 153 drive, electric regulator 154, 15
5, 0N10FF control of each of the laser light sources 2 and 3, and switching of the fluorescence detection level to be described later.

Next, the lateral optical system will be explained using FIG. Second
The figure is a side view of FIG. 1 and shows the side optical system in detail. In the figure, reference numeral 11 denotes a condensing lens, which condenses light emitted from the laser light sources 2 and 3 in a lateral direction perpendicular to the rectilinear direction of the irradiated laser beams. 13 is the field aperture,
An opening 13a is provided at the conjugate position corresponding to both the la and lb positions.
, 13b are provided. 14a and 14b are parallel flat plates, 1
5a and 15 are lenses, and the set of both members allows the lal
.

Each light from lb is converted into a parallel beam of light.

21a, 21b, 31a, and 31b are dichroic mirrors 22a, 22b, and 32a for color-separating the side scattered light and fluorescence generated by the test particles;

Reference numeral 32b represents a bandpass filter that selects the wavelength of each fluorescence, and the wavelength of each detection light is selected by the combination of this dichroic mirror and the bandpass filter. 26a, 26b, 36a.

36b are ND filters each having a predetermined transmittance. 24.34 is a lens, 25.35 is a photodetector for detecting side scattered light and fluorescence, and a photomultiplier with high detection sensitivity is suitable as the photodetector. The photodetector 25° 35 is connected to a storage/arithmetic means 61. Here, the light emitted from position 1a is transmitted through opening 13a.
Once the image is formed, dichroic mirrors 21a, 31a,
The photodetector 25.2 passes through the optical path of the bandpass filters 22a, 32a, and the ND filters 26a, 36a.
5 and reimage here. On the other hand, the light emitted from 1b is imaged once at the aperture 13b, and the dichroic mirrors 21b, 31b1 band pass filters 22b, 32b,
The photodetector 2 passes through the optical path of the ND filters 26b and 36b.
5.25 and reimage.

FIG. 3 shows a modification of the above-mentioned side optical system, and is a view of FIG. 1 viewed from above. Note that the front optical system is omitted in the figure. In the figures, the same reference numerals as in the previous figures represent the same or equivalent members.

The light emitted laterally from the laser irradiation position 1a from the laser light source 2 is once routed to an optical path in the upper part of the figure, passes through a reflection member 99 such as a corner cube or a porro prism, and is once imaged at the aperture stop 13a. , re-imaged by the photodetector 25.35 and then incident. On the other hand, the light emitted laterally from the laser irradiation position 1b from the laser light source 3 is guided to the optical path in the lower part of the figure.
Once the image is formed by the aperture stop 13b, the common photodetector 25.3
5, the image is re-imaged and detected. This example is basically the same as the second one above.
Although it is equivalent to the optical system shown in the figure, it is distinctive in that the optical path for detecting the fluorescence from 1a and the fluorescence from 1b are clearly separated and optically arranged.

FIG. 4 is an example of a detection pulse obtained by each photodetector when a particle to be detected passes through. Figure 4 (A), Figure 4 (B)
are the detection outputs of the forward scattered light obtained by the photodetectors 8a and 8b, respectively, FIG. 4(C) and FIG. 4(F) are the timing pulses created by comparing them with a predetermined threshold, and FIG.
E) and FIG. 4(F) respectively show the fluorescence detection outputs obtained by the photodetectors 25 and 35. Fluorescence detection outputs from each photodetector 25.35 are obtained in time series when the test particle passes through the la and lb positions, and these are acquired separately in time series using the previous timing pulse. It's crowded.

As described above, in this embodiment, four-channel detection is possible with two side detectors, and two forward scattered lights are added to this, making it possible to detect a total of six types of light with different optical characteristics.

In addition, although the above description has shown an example in which the number of side photodetectors is two, the number of detectors is not limited to this. If there is only one, fluorescence of two colors can be obtained with a single photodetector, as in the general conventional example, and the configuration of the device becomes extremely simple. Further, if the number of photodetectors is three or more, even more parameters can be obtained. Furthermore, what is detected by the side photodetectors is not limited to fluorescence, but may also detect side scattered light.

Now, the basic form of the system has been explained above, and next, the characteristic functions of the system of the embodiment will be explained.

(1) 1. Laser When using the system of this embodiment for multiple purposes, two laser light wavelengths may not necessarily be necessary and only one may be sufficient depending on the measurement purpose and the type of fluorescent agent used. . Alternatively, if a configuration is adopted in which laser beams from three or more types of light sources are selectively irradiated as shown in FIG. 11(D), unused laser beam wavelengths are unnecessary.

Therefore, in the system of this embodiment, multiple measurement modes are switched depending on the intended use, and the laser light source that is not in use is turned off.
It has the function of Specifically, switching between a first mode in which two laser beams are irradiated simultaneously to perform time-series measurements, and a second mode in which one of the laser beams is irradiated and the other is inactive. I can do it. This depends on the measurement conditions set in the input setting means 160.
The control means 162 controls the irradiation of each laser light source to 0N1.
0FF is performed independently to put unused laser light sources into sleep mode or to cut off the power to the laser light sources. This results in lower system power consumption and longer laser life.

(2) Since the distance between the two laser irradiation positions 1a and lb on the arm support 1 is a constant value (approximately 100 μm), the forward scattered light detector 8
Figures 4 (C) and 4 (D) are created from the outputs of a and 8b.
) The generation timings of both timing pulses shown in FIG. This timing comparison and calculation of the flow velocity are performed by the memory calculation circuit 161, and the control means 16
In step 2, this flow rate is constantly fed back to adjust the electric regulators 154 and 155 so that the flow rate is set by the input setting means 160.

This allows the set flow rate to be maintained accurately at all times, improving stability and measurement accuracy.

Note that the pressurizing mechanism is not limited to a pump and an electric regulator, and a pressurizing mechanism using a syringe may be used. In that case, adjust the extrusion speed of the syringe.

(3) Data cancellation The flow interval of the test particles flowing one after another is set to be sufficiently larger than the interval between the irradiation positions 1a and lb (about looJ, tm) in 2. In some cases, particles may flow without any interval, and each particle may approach both positions almost simultaneously. In this case, scattered light and fluorescence are generated from both positions and enter the common photodetector in a mixed manner, resulting in mixed data being detected.

Therefore, in order to prevent this, in this embodiment, la.

A means is provided for detecting that the specimens have passed through the position 1b at almost the same time and canceling the data from the detection means and not capturing it if it is determined that the specimens have passed at the same time. Specifically, in the storage calculation means 161, the fourth
If the generation timings of the timing pulses shown in FIG. 4(C) and FIG. 4(D) match or are very close to each other, la.

It is determined that two test particles have passed through both positions of 1b at almost the same time, and the data of each photodetector at this time is canceled and not captured.

Alternatively, the following method may be used.

It is considered that the time required for particles to move from la to 1b is approximately constant. There, a detection pulse (output of photodetector 8a) is generated when a particle passes through 1a, and then again during a predetermined period of time during which the particle moves to and passes through lb.
When a detection pulse is generated from a (output of photodetector 8a), it is determined that particles have continued to flow, and data acquisition is canceled.

By providing the above means, there is no possibility of capturing erroneous data, so more reliable measurements can be performed.

(4) Intensity levels of fluorescence and scattered light are generally very different, and
The amount of fluorescent light generated differs depending on the type of fluorescent dye. Therefore, in order to detect these in time series with a common photodetector, a detector with an extremely wide dynamic range is required.

Therefore, in this embodiment, in each optical path leading to the photodetector, there are passing light amount adjusting means (ND filters 26a, 26b) for adjusting the amount of passing light according to the amount of fluorescent light used.

36a and 36b) are provided, respectively, so that the difference in the amount of light incident on the photodetector is small. This eliminates the need to use an expensive photodetector with a large dynamic range, resulting in a lower cost system.

Note that the amount of passing light may be adjusted not only by the ND filter but also by an optical mask that limits the light-blocking area of the optical path.

Furthermore, if a mechanism for replacing the ND filter or a mechanism for freely adjusting the amount of light passing through is provided, and this is adjusted according to the fluorescent dye used, the system becomes more versatile.

(5) Level change 2 In this embodiment, regarding the above-mentioned intensity level switching, in addition to the ND filter, there is also a circuit for switching the amplification factor of the photodetector depending on the type of measurement light. This is the memory calculation circuit 16
When receiving the output of the photodetector 25.35, a circuit is installed to switch the gain of the amplifier in synchronization with the passage of the la and lb particles, so that the detection sensitivity is switched according to the amount of light emitted. ing. This gain can be adjusted freely to accommodate a wide range of measurements, and the input setting means 16
The amplification gain is determined according to the measurement conditions input from 0. Now, although the above two embodiments are examples of the basic configuration of the present invention, some more specific usage examples will be described below. It goes without saying that the types and combinations of fluorescent dyes that can be used are not limited to these.

In this example, a specimen is simultaneously stained with three types of fluorescent dyes, and four channels are detected using two photodetectors in the side optical system.

In Figures 1 and 2, laser light sources 2 and 3 have a wavelength of 4
Two 88 nm -Ar+ laser light sources are used.

As the type of fluorescent dye that stains the test particles, fluorescence excited by excitation light with a wavelength of 488 nm is selected. For example, FITC (
530nm), PE (570nm), DCH-d=l
Assuming that staining is performed using three types of fluorescence: ÷ (610 nm), 488 nm and 530 nm from the test particle.

Light of four different wavelengths, 570 nm and 610 nm, is generated simultaneously. Note that the DC is not directly excited by the wavelength of 488 nm, but has a stepwise excitation process in which the DC is excited by the fluorescence (570 nm) generated when PE is excited.

After staining the specimen with the three types of fluorescence described above as pretreatment, measurement is performed using the apparatus of this example. Here, the light separation wavelengths of the dichroic mirrors 21a, 21b, 31a, and 31b are 510 nm and 590 nm, respectively.

Band pass filters 22 a, 22 b, 32 a, 32 are set to about 450 nm and 650 nm.
b as 530 nm, 488 nm, and 570 nm, respectively.
.

FITC is used in each optical system using a material that selectively transmits light with a wavelength around 610 nm.

Intensity detection of SS (side scattered light), PE, and DC is performed.

When the test particle passes through the position 1a, the four types of light mentioned above are generated. At this time, the fluorescence intensity of FITC selected by the bandpass filter 22a is detected in the detector 25, and the detector At step 35, the fluorescence intensity of the PE selected by the bandpass filter 32a is detected. Next, when the same test particle passes through the position 1b, the handrails 23a, 33a are closed, 23b,
33b opens, the detector 25 detects the SS intensity selected by the bandpass filter 22b, and the detector 35 detects the DC fluorescence intensity selected by the bandpass filter 32b.

In this way, measurements of light with four different optical characteristics can be obtained using two detectors. In this, photodetectors 8a and 8b
By adding the two types of forward scattered light intensities obtained in , a total of six types of measurement parameters can be obtained.

An example of use will be described in which 6-channel detection is possible, in which specimens simultaneously stained with four types of fluorescent dyes can be measured using the first and second side optical systems.

In the optical system that includes three photodetectors by adding another side detection system to the configuration shown in Fig. 2, the laser light source 2 is
An Ar+ laser light source with a wavelength of 488 nm is used as the laser light source 3, and a dye laser light source with a wavelength of 600 nm is used as the laser light source 3.

The type of fluorescent dye that stains the test particles has a wavelength of 488 nm.
The fluorescence excited by the excitation light and the fluorescence excited by the wavelength of 600 nm are selected. For example, FITC (530nm) and PE (570nm) are used as those suitable for 488nm, and TR (610nm) is used as those suitable for 600nm.
) and APC (660 nm), and stain with a total of four types of fluorescence.

After staining the specimen with the four types of fluorescence described above as pretreatment, measurement is performed using the apparatus of this example. Here, the light separation wavelengths of the dichroic mirrors 21a, 21b, 31a, and 31b are 570 nm and 605 nm, respectively.

Band pass filters 22 a, 22 b, 32 a, 32 are set to about 550 nm and 630 nm.
b, 42 a.

42b is 488 nm and 600 nm, respectively.

A material having a characteristic of selectively transmitting light having wavelengths around 530 nm, 610 nm, 570 nm, and 660 nm is selected.

When the test particles pass through position 1a where Ar+ laser light is irradiated, FITC is performed with 488 nm irradiation light.

PE is excited, 488nm, 530nm, 570nm
Three types of light are generated. At this time .

-1゜゛ SS (488nm) at detector 25
However, the detector 35 detects FITC and the detector 45 detects PE. Next, when the same test particle passes the lb position irradiated with the dye laser light after a predetermined time,
TR with 600 nm irradiation light.

APC is excited, 600nm, 610nm, 660n
Three types of light are generated. At this point, SS (600 nm) is detected by the detector 25, TR is detected by the detector 35, and APC is detected by the detector 45. As described above, in this example, it is possible to detect a total of 6 channels, 2 channels of side scattered light and 4 channels of fluorescent light, with the 3 side detectors, and a total of 7 types of different optical characteristics including forward scattered light. light becomes detectable.

Similar to Usage Example 2 above, another usage example will be described in which a sample simultaneously stained with four types of fluorescent dyes can be measured using a side optical system and 6-channel detection is possible.

A He-Ne laser light source with a wavelength of 633 nm was used as the laser light source 2, and an Ar+ laser light source with a wavelength of 488 nm was used as the light source 3, and the types of fluorescent dyes used to stain the test particles were 6.
APC (660n) is suitable for 33nm excitation light.
m), select UL'i (695nm), 4
FITC (530 nm) is suitable for 88 nm excitation light.
nm) and PI (620 nm), and multiple stain the specimen with these four types of fluorescent dyes.

and dichroic mirrors 21a and 21b.

The optical separation wavelength of 31a and 31b is 640 nm, respectively.

Bandpass filters 22a, 22b, and 32a are set to approximately 500nm, 670nm, and 550nm.

-633 nm as 32b, 42a, and 42b, respectively.

488nm, 660nm, 520nm, 695nm.

A material having a characteristic of selectively transmitting light with a wavelength around 62 Onm is used.

As a result, similarly to Usage Example 2, six channels can be detected with the side optical system having three photodetectors.

[Effects of the Invention] As described above, according to the present invention, by deactivating the light source of the beam that is not used depending on the measurement mode, it is possible to provide an apparatus with low power consumption and a long life.

[Brief explanation of the drawing]

Fig. 1 is a configuration diagram of an embodiment of the present invention, Fig. 2 is a configuration diagram of a side optical system of the embodiment, Fig. 3 is a diagram of a modification of the lateral optical system, and Fig. 4 is an embodiment. Figure 5 is a diagram of a modified example of the irradiation optical system, Figure 6 is a configuration diagram of a conventional example, and the main symbols in the diagram are: 1...flow cell, 2.3. ...Laser light source, 8...Photodetector, 22.32...Band pass filter, 25.35.
...Photodetector, 26.36...ND filter, 5th section of whistle

Claims (3)

[Claims] (1) a setting means for setting a measurement mode; a first irradiation means for generating a first irradiation light and irradiating it to a first position where the specimen moves; and a first irradiation means for generating a second irradiation light and for irradiating the first The second position is different from the position of
a second irradiation means that irradiates the position, and a light detection means that detects light having first and second optical characteristics emitted from the specimen passing through the first and second positions in time series using the same photodetector. A control means for controlling whether or not the first irradiation means and the second irradiation means are activated according to the set measurement mode. (2) The control means, depending on the settings of the setting means,
The method according to claim (1), wherein the mode is switched between a mode in which one of the first and second irradiation means is activated and the other is inactivated, and a mode in which both the first and second irradiation means are activated. Specimen measurement device. (3) The first irradiation means and the second irradiation means each have a laser light source, and when the irradiation means is inactive, the laser light source is put into sleep mode or the power of the laser light source is cut off ( 1) The specimen measuring device described above.