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JP2008194050A - 宿主細胞でのｐａｃｅの発現およびその使用法 - Google Patents

宿主細胞でのｐａｃｅの発現およびその使用法 Download PDF

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JP2008194050A
JP2008194050A JP2008066877A JP2008066877A JP2008194050A JP 2008194050 A JP2008194050 A JP 2008194050A JP 2008066877 A JP2008066877 A JP 2008066877A JP 2008066877 A JP2008066877 A JP 2008066877A JP 2008194050 A JP2008194050 A JP 2008194050A
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compositions
methods
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expression
host cells
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Philip J Barr
ジェー． バール フィリップ
Anthony J Brake
ジェー． ブレイク アンソニー
Randal J Kaufman
ジェー． コーフマン ランダル
Patricia Tekamp-Olson
テカムプ−オルソン パトリシア
Louise Wasley
ワスレー ルイーズ
Polly A Wong
エイ． ウォン ポリー
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Novartis Vaccines and Diagnostics Inc
Genetics Institute LLC
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Novartis Vaccines and Diagnostics Inc
Genetics Institute LLC
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Application filed by Novartis Vaccines and Diagnostics Inc, Genetics Institute LLC filed Critical Novartis Vaccines and Diagnostics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6454—Dibasic site splicing serine proteases, e.g. kexin (3.4.21.61); furin (3.4.21.75) and other proprotein convertases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

【課題】エンドペプチダーゼを生産するための組成物および方法を提供すること。
【解決手段】エンドペプチダーゼを生産するための組成物および方法、並びに、生物学的活性のためにγ−カルボキシル化を必要とするタンパク質を含む、異種前駆体ポリペプチドの成熟ポリペプチドへのプロセシングの効率を高めるための組成物および方法を提供する。これらの組成物および方法は、二塩基性アミノ酸部位に特異的に作用する哺乳動物のエンドペプチダーゼである組換えＰＡＣＥを利用するものである。
【選択図】なし

Description

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発明の概要
本発明は、例えば以下の項目を提供する。
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図１は、Ａ．Ｍ．Ｗ． ｖａｎ ｄｅｎ Ｏｕｗｅｌａｎｄ ｅｔ． ａｌ．， Ｎｕｃｌ． Ａｃｉｄｓ Ｒｅｓ．， １８（３）：６６４ （１９９０）に発表されたｆｕｒＤＮＡ配列を示す。 図１の続き。 図１の続き。 図１の続き。 図１の続き。 図１の続き。 図２は、ＰＡＣＥをコードする完全なｃＤＮＡ配列、およびそこにコードされるアミノ酸を示し、これはヌクレオチド−３２０から−１までの５’非翻訳領域とヌクレオチド２３８３から３９７４までの３’非翻訳領域を含む点で上記図１と相違する。 図２の続き。 図２の続き。 図２の続き。 図２の続き。 図２の続き。 図２の続き。 図２の続き。 図２の続き。 図２の続き。 図２の続き。

Claims (1)

  1. 明細書中に記載の、組成物。
JP2008066877A 1990-11-26 2008-03-14 宿主細胞でのｐａｃｅの発現およびその使用法 Withdrawn JP2008194050A (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US62109290A 1990-11-26 1990-11-26
US62144390A 1990-11-29 1990-11-29
US62085990A 1990-11-29 1990-11-29
US62145790A 1990-11-30 1990-11-30

Related Parent Applications (1)

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JP50154992A Division JP4236698B2 (ja) 1990-11-26 1991-11-26 宿主細胞でのｐａｃｅの発現およびその使用法

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JP50154992A Expired - Lifetime JP4236698B2 (ja) 1990-11-26 1991-11-26 宿主細胞でのｐａｃｅの発現およびその使用法
JP2008066877A Withdrawn JP2008194050A (ja) 1990-11-26 2008-03-14 宿主細胞でのｐａｃｅの発現およびその使用法

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EP (2) EP0574402B2 (ja)
JP (2) JP4236698B2 (ja)
AT (1) ATE158816T1 (ja)
CA (1) CA2096418C (ja)
DE (1) DE69127829T2 (ja)
DK (1) DK0574402T3 (ja)
ES (1) ES2109336T3 (ja)
IE (1) IE914102A1 (ja)
WO (1) WO1992009698A1 (ja)

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EP0574402A1 (en) 1993-12-22
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JP4236698B2 (ja) 2009-03-11
ES2109336T3 (es) 1998-01-16
CA2096418A1 (en) 1992-05-27
ATE158816T1 (de) 1997-10-15
EP0574402A4 (en) 1994-08-24
US5986079A (en) 1999-11-16
EP0574402B1 (en) 1997-10-01
DK0574402T3 (da) 1998-05-18
EP0574402B2 (en) 2012-08-01
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US5965425A (en) 1999-10-12
US5460950A (en) 1995-10-24

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