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FI884904A0 - Molekylaerkloning och uttryckning av gener, som kodar foer proteolytiska enzymer. - Google Patents

Molekylaerkloning och uttryckning av gener, som kodar foer proteolytiska enzymer.

Info

Publication number
FI884904A0
FI884904A0 FI884904A FI884904A FI884904A0 FI 884904 A0 FI884904 A0 FI 884904A0 FI 884904 A FI884904 A FI 884904A FI 884904 A FI884904 A FI 884904A FI 884904 A0 FI884904 A0 FI 884904A0
Authority
FI
Finland
Prior art keywords
bacillus
protoplasts
dna
bacillus cells
dna sequence
Prior art date
Application number
FI884904A
Other languages
English (en)
Other versions
FI105483B (fi
FI884904A (fi
Inventor
Eekelen Christiaan A G Van
Der Laan Johannes C Van
Leonardus J S M Mulleners
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of FI884904A publication Critical patent/FI884904A/fi
Publication of FI884904A0 publication Critical patent/FI884904A0/fi
Priority to FI925413A priority Critical patent/FI106726B/fi
Application granted granted Critical
Publication of FI105483B publication Critical patent/FI105483B/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FI884904A 1987-02-27 1988-10-24 Menetelmä kiinnostavan endogeenisen entsyymin tuotannon tehostamiseksi alkalofiilisessa Bacillus-isäntäsolussa FI105483B (fi)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FI925413A FI106726B (fi) 1987-02-27 1992-11-27 Korkea-alkalisia proteaaseja koodaavien geenien transformointi Bacillus-bakteereihin, ja menetelmässä käytettävät vektorit, DNA-sekvenssit ja koettimet

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP87200358 1987-02-27
EP87200358 1987-02-27
NL8800007 1988-02-26
PCT/NL1988/000007 WO1988006624A2 (en) 1987-02-27 1988-02-26 Molecular cloning and expression of genes encoding proteolytic enzymes

Publications (3)

Publication Number Publication Date
FI884904A FI884904A (fi) 1988-10-24
FI884904A0 true FI884904A0 (fi) 1988-10-24
FI105483B FI105483B (fi) 2000-08-31

Family

ID=8197584

Family Applications (3)

Application Number Title Priority Date Filing Date
FI884904A FI105483B (fi) 1987-02-27 1988-10-24 Menetelmä kiinnostavan endogeenisen entsyymin tuotannon tehostamiseksi alkalofiilisessa Bacillus-isäntäsolussa
FI925413A FI106726B (fi) 1987-02-27 1992-11-27 Korkea-alkalisia proteaaseja koodaavien geenien transformointi Bacillus-bakteereihin, ja menetelmässä käytettävät vektorit, DNA-sekvenssit ja koettimet
FI20001152A FI119028B (fi) 1987-02-27 2000-05-15 Menetelmä alkalofiilisten Bacillus-kantojen transformoimiseksi

Family Applications After (2)

Application Number Title Priority Date Filing Date
FI925413A FI106726B (fi) 1987-02-27 1992-11-27 Korkea-alkalisia proteaaseja koodaavien geenien transformointi Bacillus-bakteereihin, ja menetelmässä käytettävät vektorit, DNA-sekvenssit ja koettimet
FI20001152A FI119028B (fi) 1987-02-27 2000-05-15 Menetelmä alkalofiilisten Bacillus-kantojen transformoimiseksi

Country Status (19)

Country Link
US (1) US5217878A (fi)
JP (1) JP3026567B2 (fi)
KR (1) KR0127560B1 (fi)
CN (1) CN88101681A (fi)
AT (2) ATE181110T1 (fi)
AU (1) AU620026B2 (fi)
BG (1) BG49049A3 (fi)
BR (1) BR8805647A (fi)
CA (1) CA1339100C (fi)
DE (2) DE3856339T2 (fi)
DK (2) DK175738B1 (fi)
FI (3) FI105483B (fi)
HU (1) HU213220B (fi)
LT (1) LT4000B (fi)
LV (1) LV10307B (fi)
NO (1) NO884423L (fi)
PT (1) PT86864B (fi)
RU (1) RU2023723C1 (fi)
WO (1) WO1988006624A2 (fi)

Families Citing this family (51)

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WO1988006624A2 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Molecular cloning and expression of genes encoding proteolytic enzymes
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
GB2214508A (en) * 1988-01-27 1989-09-06 Bayer Ag Plasmid vector for the efficient expression of foreign genes fused with newly conceived transcriptional and translational signal sequences.
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
HUT53154A (en) * 1988-05-05 1990-09-28 New York Health Res Inst Process for producing protease-defective gram-positive bacteria and utilizing them as host-organizmus for producing recombinant products
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
EP0571014B1 (en) * 1992-05-18 2004-03-31 Genencor International, Inc. Bacteria producing alkaline proteases, and production of these alkaline proteases
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5831004A (en) 1994-10-27 1998-11-03 Affymax Technologies N.V. Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6300116B1 (en) 1996-11-04 2001-10-09 Novozymes A/S Modified protease having improved autoproteolytic stability
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US6485957B1 (en) 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6458564B1 (en) 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
US6426199B1 (en) 1999-08-31 2002-07-30 Ortho-Mcneil Pharmaceutical, Inc. Dna
CN1312370A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
AU4981101A (en) * 2000-04-03 2001-10-15 Maxygen Inc Subtilisin variants
JP4680512B2 (ja) 2002-03-29 2011-05-11 ジェネンコー・インターナショナル・インク バチルスにおけるタンパク質発現の増大
WO2004101760A2 (en) * 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme elip
EP1625208A4 (en) * 2003-05-12 2006-10-18 Genencor Int NEW LIPOLYTIC ENZYME LIP2
WO2004101763A2 (en) 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme lip1
EP1667530A4 (en) 2003-09-19 2011-05-25 Ics Holdings Inc MANUFACTURE OF A COURABLE PRODUCT OF DOUGH
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
KR101482015B1 (ko) * 2003-11-19 2015-01-23 다니스코 유에스 인크. 세린 프로테아제, 세린 효소들을 인코딩하는 핵산 및 이를편입시킨 벡터 및 숙주 세포
EP1735339A2 (en) * 2004-04-09 2006-12-27 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
EP1766002B1 (en) * 2004-06-21 2015-11-18 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
US7202074B2 (en) * 2004-07-22 2007-04-10 University Of Chile Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
WO2008112258A2 (en) 2007-03-12 2008-09-18 Danisco Us Inc. Modified proteases
EP2460823B1 (en) 2007-05-10 2014-02-26 Danisco US Inc. A modified secretion system to increase expression of polypeptides in bacteria
EP2173873B1 (en) 2007-08-06 2015-11-18 University of Chile Protein and dna sequence encoding a cold adapted subtilisin-like activity
JP5629584B2 (ja) 2007-12-21 2014-11-19 ダニスコ・ユーエス・インク バチルス内での促進されたタンパク質生成
FI123122B (fi) 2009-02-16 2012-11-15 Optogear Oy Laitteisto lasimateriaalin valmistamiseksi
CA2759695C (en) * 2009-04-24 2018-05-01 Danisco Us Inc. Proteases with modified pro regions
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
MX350391B (es) * 2011-09-22 2017-09-06 Novozymes As Polipeptidos que poseen actividad proteasa y polinucleotidos que los codifican.
CN103013960A (zh) * 2012-12-21 2013-04-03 青岛蔚蓝生物集团有限公司 一种碱性蛋白酶及其重组表达工程菌
DK3089991T3 (da) 2013-12-31 2019-12-02 Danisco Us Inc Forbedret proteinekspression
EP3233893B1 (en) 2014-12-16 2021-01-20 Danisco US Inc. Enhanced protein expression
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WO2018202911A1 (en) * 2017-05-05 2018-11-08 Bioecho Life Sciences Gmbh Rapid purification of high quality nucleic acids from biological samples
US12139715B2 (en) 2018-06-19 2024-11-12 Seoul National University R&Db Foundation Gene expression cassette capable of initiating translation after completion of transcription for producing high-quality recombinant protein in bacteria

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Also Published As

Publication number Publication date
ATE180016T1 (de) 1999-05-15
WO1988006624A3 (en) 1988-11-17
FI105483B (fi) 2000-08-31
DE3856339T2 (de) 1999-11-04
CN88101681A (zh) 1988-11-09
HU213220B (en) 1997-03-28
FI925413A0 (fi) 1992-11-27
US5217878A (en) 1993-06-08
BR8805647A (pt) 1989-10-31
AU620026B2 (en) 1992-02-13
LTIP1820A (en) 1995-08-25
DK175738B1 (da) 2005-02-07
CA1339100C (en) 1997-07-29
DE3856331T2 (de) 1999-10-14
DE3856331D1 (de) 1999-06-17
KR890700665A (ko) 1989-04-26
PT86864B (pt) 1992-05-29
BG49049A3 (en) 1991-07-15
DK593988A (da) 1988-10-26
FI119028B (fi) 2008-06-30
AU1398088A (en) 1988-09-26
LV10307A (lv) 1994-10-20
FI925413A (fi) 1992-11-27
FI884904A (fi) 1988-10-24
WO1988006624A2 (en) 1988-09-07
JP3026567B2 (ja) 2000-03-27
LT4000B (en) 1996-06-25
NO884423D0 (no) 1988-10-05
LV10307B (en) 1995-08-20
RU2023723C1 (ru) 1994-11-30
FI106726B (fi) 2001-03-30
PT86864A (pt) 1988-03-01
JPH01502876A (ja) 1989-10-05
ATE181110T1 (de) 1999-06-15
DE3856339D1 (de) 1999-07-15
DK200401262A (da) 2004-08-20
DK175852B1 (da) 2005-04-04
KR0127560B1 (ko) 1997-12-29
NO884423L (no) 1988-10-05
FI20001152A (fi) 2000-05-15
DK593988D0 (da) 1988-10-26

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Owner name: GENENCOR INTERNATIONAL, INC.

FG Patent granted

Owner name: GENENCOR INTERNATIONAL, INC.