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AR076941A1 - Cepa de bacillus para una mayor produccion de proteina - Google Patents

Cepa de bacillus para una mayor produccion de proteina

Info

Publication number
AR076941A1
AR076941A1 ARP100101924A ARP100101924A AR076941A1 AR 076941 A1 AR076941 A1 AR 076941A1 AR P100101924 A ARP100101924 A AR P100101924A AR P100101924 A ARP100101924 A AR P100101924A AR 076941 A1 AR076941 A1 AR 076941A1
Authority
AR
Argentina
Prior art keywords
bacillus
host cells
cepa
protein production
interest
Prior art date
Application number
ARP100101924A
Other languages
English (en)
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of AR076941A1 publication Critical patent/AR076941A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Células huésped que han sido manipuladas genéticamente para tener una mejor capacidad para producir proteínas de interés. En particular, se refiere a células huésped de Bacillus sp. que tienen al menos un gen de phr inactivado. El aumento de produccion de las proteínas de interés por las células huésped de Bacillus sp. modificadas está adicionalmente aumentado en las células huésped de Bacillus sp. modificadas que sobreexpresan YmaH. También se proporcionan métodos para producir proteínas de interés en las células huésped modificadas.
ARP100101924A 2009-06-11 2010-06-01 Cepa de bacillus para una mayor produccion de proteina AR076941A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US18632109P 2009-06-11 2009-06-11

Publications (1)

Publication Number Publication Date
AR076941A1 true AR076941A1 (es) 2011-07-20

Family

ID=42562872

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP100101924A AR076941A1 (es) 2009-06-11 2010-06-01 Cepa de bacillus para una mayor produccion de proteina

Country Status (6)

Country Link
US (2) US8293499B2 (es)
EP (1) EP2440573B1 (es)
CN (1) CN102803290B (es)
AR (1) AR076941A1 (es)
DK (1) DK2440573T3 (es)
WO (1) WO2010144283A1 (es)

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WO2013086219A1 (en) * 2011-12-09 2013-06-13 Danisco Us Inc. Ribosomal promotors from b. subtilis for protein production in microorganisms
US10287591B2 (en) 2013-12-31 2019-05-14 Danisco Us Inc Enhanced protein expression
KR101619316B1 (ko) 2014-05-27 2016-05-11 한국생명공학연구원 바실러스 프로모터 변이체 및 이를 이용한 단백질의 제조방법
DE102014223904A1 (de) * 2014-11-24 2016-05-25 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Kondensator und Verfahren zum Herstellen desselben
US10683528B2 (en) 2014-12-16 2020-06-16 Danisco Us Inc Enhanced protein expression
JP2017538433A (ja) * 2014-12-19 2017-12-28 ダニスコ・ユーエス・インク タンパク質の発現増大
FI3259358T3 (fi) * 2015-02-19 2024-09-17 Danisco Us Inc Tehostunut proteiiniekspressio
US11306319B2 (en) 2015-10-30 2022-04-19 Danisco Us Inc. Enhanced protein expression and methods thereof
US20180371445A1 (en) * 2015-12-23 2018-12-27 Danisco Us Inc. Enhanced protein production and methods thereof
JP7103948B2 (ja) 2016-03-04 2022-07-20 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター
CN108456669B (zh) * 2017-02-20 2023-08-08 上海凯赛生物技术股份有限公司 一种核糖体结合位点、重组表达质粒、转化子及其应用
KR102715197B1 (ko) * 2018-01-03 2024-10-08 다니스코 유에스 인크. 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법
CN109355303B (zh) * 2018-11-14 2022-05-06 天津大学 抑制和/或敲除基因在提高单克隆抗体的表达量中的应用
JP2022509215A (ja) * 2018-11-28 2022-01-20 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞におけるタンパク質産生増強のための新規なプロモーター配列及びその方法
CN114127256A (zh) * 2019-02-20 2022-03-01 巴斯夫欧洲公司 用确定成分培养基和微量元素补料的芽孢杆菌工业发酵工艺
US20220186177A1 (en) * 2019-02-20 2022-06-16 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
CN111004815B (zh) * 2019-12-18 2023-07-21 江苏省农业科学院 通过突变degU基因提高枯草芽孢杆菌表面活性素产量的方法
CN112646746B (zh) * 2021-01-04 2022-02-25 济南大学 一株高效降解驴毛的坚强芽孢杆菌
CN112941088B (zh) * 2021-02-04 2023-06-16 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） 与布氏杆菌毒力相关的基因及其在布氏杆菌毒力评价及制备弱毒布氏杆菌中的应用
CN115260314B (zh) * 2022-05-10 2023-10-24 吉林大学 一种重组嗜热中性蛋白酶及其在降解蛋白质中的应用
CN116584507A (zh) * 2023-03-13 2023-08-15 湖南人文科技学院 一种防治油茶叶斑病的茶枯酵素及其应用

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Also Published As

Publication number Publication date
US8476042B2 (en) 2013-07-02
DK2440573T3 (en) 2015-01-19
US20120142076A1 (en) 2012-06-07
US8293499B2 (en) 2012-10-23
US20130029402A1 (en) 2013-01-31
EP2440573B1 (en) 2014-10-15
EP2440573A1 (en) 2012-04-18
WO2010144283A1 (en) 2010-12-16
CN102803290A (zh) 2012-11-28
CN102803290B (zh) 2015-11-25

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