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AU2001279254A1 - Bacillus transformation, transformants and mutant libraries - Google Patents

Bacillus transformation, transformants and mutant libraries

Info

Publication number
AU2001279254A1
AU2001279254A1 AU2001279254A AU7925401A AU2001279254A1 AU 2001279254 A1 AU2001279254 A1 AU 2001279254A1 AU 2001279254 A AU2001279254 A AU 2001279254A AU 7925401 A AU7925401 A AU 7925401A AU 2001279254 A1 AU2001279254 A1 AU 2001279254A1
Authority
AU
Australia
Prior art keywords
transformants
mutant libraries
bacillus transformation
bacillus
transformation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001279254A
Inventor
Maria R. Diaz-Torres
Edwin W. Lee
Thomas B. Morrison
Volker Schellenberger
Olga V. Selifonova
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22842887&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2001279254(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of AU2001279254A1 publication Critical patent/AU2001279254A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Steroid Compounds (AREA)

Abstract

The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.
AU2001279254A 2000-08-11 2001-08-10 Bacillus transformation, transformants and mutant libraries Abandoned AU2001279254A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22494800P 2000-08-11 2000-08-11
US60/224,948 2000-08-11
PCT/US2001/025166 WO2002014490A2 (en) 2000-08-11 2001-08-10 Bacillus transformation, transformants and mutant libraries

Publications (1)

Publication Number Publication Date
AU2001279254A1 true AU2001279254A1 (en) 2002-02-25

Family

ID=22842887

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001279254A Abandoned AU2001279254A1 (en) 2000-08-11 2001-08-10 Bacillus transformation, transformants and mutant libraries

Country Status (8)

Country Link
US (2) US20020182734A1 (en)
EP (1) EP1309677B2 (en)
AT (1) ATE400648T1 (en)
AU (1) AU2001279254A1 (en)
CA (1) CA2418317A1 (en)
DE (1) DE60134752D1 (en)
DK (1) DK1309677T4 (en)
WO (1) WO2002014490A2 (en)

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Also Published As

Publication number Publication date
EP1309677B2 (en) 2012-04-11
US20060199222A1 (en) 2006-09-07
DE60134752D1 (en) 2008-08-21
US20020182734A1 (en) 2002-12-05
EP1309677A2 (en) 2003-05-14
EP1309677B1 (en) 2008-07-09
ATE400648T1 (en) 2008-07-15
DK1309677T3 (en) 2008-11-03
DK1309677T4 (en) 2012-06-25
CA2418317A1 (en) 2002-02-21
WO2002014490A2 (en) 2002-02-21
WO2002014490A3 (en) 2003-02-06

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