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AU2003223928A1 - Homologous recombination into bacterium for the generation of polynucleotide libraries - Google Patents

Homologous recombination into bacterium for the generation of polynucleotide libraries

Info

Publication number
AU2003223928A1
AU2003223928A1 AU2003223928A AU2003223928A AU2003223928A1 AU 2003223928 A1 AU2003223928 A1 AU 2003223928A1 AU 2003223928 A AU2003223928 A AU 2003223928A AU 2003223928 A AU2003223928 A AU 2003223928A AU 2003223928 A1 AU2003223928 A1 AU 2003223928A1
Authority
AU
Australia
Prior art keywords
bacterium
generation
homologous recombination
polynucleotide libraries
libraries
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003223928A
Inventor
Mads Eskelund Bjornvad
Peter Kamp Hansen
Per Lina Jorgensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of AU2003223928A1 publication Critical patent/AU2003223928A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1082Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003223928A 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries Abandoned AU2003223928A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200200682 2002-05-07
DKPA200200682 2002-05-07
PCT/DK2003/000301 WO2003095658A1 (en) 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries

Publications (1)

Publication Number Publication Date
AU2003223928A1 true AU2003223928A1 (en) 2003-11-11

Family

ID=29414623

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003223928A Abandoned AU2003223928A1 (en) 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries

Country Status (2)

Country Link
AU (1) AU2003223928A1 (en)
WO (1) WO2003095658A1 (en)

Cited By (1)

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CN108165516A (en) * 2018-03-22 2018-06-15 江南大学 A kind of method of fermentation of bacillus subtilis production propylhomoserin dehydrogenase

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ES2380105T3 (en) 2004-06-21 2012-05-08 Novozymes A/S Nocardiopsis proteases
US20080038780A1 (en) * 2006-02-15 2008-02-14 Novozymes Biopolymer A/S Production of low molecular weight hyaluronic acid
ES2720369T3 (en) 2008-06-06 2019-07-19 Procter & Gamble Detergent composition comprising a variant of a family xyloglucanase 44
EP2674488A1 (en) 2008-06-06 2013-12-18 Novozymes A/S Variants of a family 44 xyloglucanase
DE102008052529A1 (en) * 2008-10-21 2010-04-22 Henkel Ag & Co. Kgaa Expression-enhanced nucleic acids
US8409830B2 (en) 2009-08-21 2013-04-02 Novozymes A/S Polypeptides having isoamylase activity and methods of use
EP3550016B1 (en) 2009-11-06 2022-05-04 Novozymes, Inc. Composition for saccharification of cellulosic material
US20120329090A1 (en) 2009-12-21 2012-12-27 Novozymes A/S Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells
EP2539447B1 (en) 2010-02-25 2017-07-26 Novozymes A/S Variants of a lysozyme and polynucleotides encoding same
CN102884182A (en) 2010-03-03 2013-01-16 诺维信股份有限公司 Xylanase variants and polynucleotides encoding same
EP2647710A1 (en) 2010-04-09 2013-10-09 Eviagenics S.A. Method of generating gene mosaics
EP2616483A1 (en) 2010-09-16 2013-07-24 Novozymes A/S Lysozymes
EP2744900B1 (en) 2011-08-19 2017-07-19 Novozymes A/S Polypeptides having protease activity
US20140274803A1 (en) 2011-10-12 2014-09-18 Eviagenics S.A. Method of generating gene mosaics in eukaryotic cells
ES2667318T3 (en) 2011-12-28 2018-05-10 Novozymes A/S Polypeptides with protease activity
DK2847308T3 (en) 2012-05-07 2017-10-23 Novozymes As Polypeptides with xanthan-degrading activity and polynucleotides encoding them
US20150140165A1 (en) 2012-06-20 2015-05-21 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
WO2014096259A1 (en) 2012-12-21 2014-06-26 Novozymes A/S Polypeptides having protease activiy and polynucleotides encoding same
CN105378079B (en) * 2013-07-12 2019-09-27 诺维信公司 Direct transfer of polynucleotides between genomes
US10030239B2 (en) 2013-12-20 2018-07-24 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
EP3234143B1 (en) 2014-12-19 2023-06-28 Novozymes A/S Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
WO2016196202A1 (en) 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
US10450539B2 (en) 2015-06-04 2019-10-22 Novozymes A/S Use of M4 metalloprotease in wort production
CN108350441B (en) 2015-10-07 2022-09-27 诺维信公司 Polypeptides
WO2017064253A1 (en) 2015-10-14 2017-04-20 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
WO2017089366A1 (en) 2015-11-24 2017-06-01 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
EP3408386A1 (en) 2016-01-29 2018-12-05 Novozymes A/S Beta-glucanase variants and polynucleotides encoding same
US10947520B2 (en) 2016-05-24 2021-03-16 Novozymes A/S Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity
CN109153981A (en) 2016-05-24 2019-01-04 诺维信公司 Polypeptide with alpha-galactosidase activity and the polynucleotides for encoding it
CN109196098A (en) 2016-05-24 2019-01-11 诺维信公司 Composition comprising the polypeptide with galactanase activity and the polypeptide with betagalactosidase activity
KR102507692B1 (en) 2016-08-24 2023-03-09 헨켈 아게 운트 코. 카게아아 Detergent composition comprising GH9 endoglucanase variant I
WO2018037062A1 (en) 2016-08-24 2018-03-01 Novozymes A/S Gh9 endoglucanase variants and polynucleotides encoding same
CN110072997A (en) 2016-11-23 2019-07-30 诺维信公司 Polypeptide with proteinase activity and the polynucleotides for encoding it
US11149233B2 (en) 2017-03-31 2021-10-19 Novozymes A/S Polypeptides having RNase activity
EP3601549A1 (en) 2017-03-31 2020-02-05 Novozymes A/S Polypeptides having dnase activity
WO2019038059A1 (en) 2017-08-24 2019-02-28 Henkel Ag & Co. Kgaa Detergent compositions comprising gh9 endoglucanase variants ii
CN111278971A (en) 2017-08-24 2020-06-12 诺维信公司 GH9 endoglucanase variants and polynucleotides encoding same
AR112955A1 (en) 2017-09-01 2020-01-08 Novozymes As ANIMAL FEED ADDITIVES INCLUDING POLYPEPTIDES HAVING PROTEASE ACTIVITY AND USES OF THE SAME
US11414814B2 (en) 2017-09-22 2022-08-16 Novozymes A/S Polypeptides
EP3692147A1 (en) 2017-10-02 2020-08-12 Novozymes A/S Polypeptides having mannanase activity and polynucleotides encoding same
EP3692148A1 (en) 2017-10-02 2020-08-12 Novozymes A/S Polypeptides having mannanase activity and polynucleotides encoding same
US20200248159A1 (en) 2017-10-04 2020-08-06 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
WO2019081515A1 (en) 2017-10-24 2019-05-02 Novozymes A/S Compositions comprising polypeptides having mannanase activity
WO2019096903A1 (en) 2017-11-20 2019-05-23 Novozymes A/S New galactanases (ec 3.2.1.89) for use in soy processing
US20210102184A1 (en) 2018-02-23 2021-04-08 Henkel Ag & Co. Kgaa Detergent composition comprising xanthan lyase and endoglucanase variants
US11473076B2 (en) 2018-08-31 2022-10-18 Novozymes A/S Animal feed additives and compositions comprising an S8 serine protease
US11993762B2 (en) 2018-10-03 2024-05-28 Novozymes A/S Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same
EP3947619A1 (en) 2019-04-03 2022-02-09 Novozymes A/S Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions
EP4077656A2 (en) 2019-12-20 2022-10-26 Novozymes A/S Polypeptides having proteolytic activity and use thereof
EP4103709A2 (en) 2020-02-10 2022-12-21 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
WO2021163015A1 (en) 2020-02-10 2021-08-19 Novozymes A/S Process for producing ethanol from raw starch using alpha-amylase variants
US20230142991A1 (en) 2020-02-10 2023-05-11 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2022161914A1 (en) 2021-01-26 2022-08-04 Basf Se High temperature fermentation process and microorganisms
AU2022422537A1 (en) 2021-12-22 2024-06-06 Novozymes A/S Method of producing a milk-based product
AU2023231428A1 (en) 2022-03-08 2024-07-11 Novozymes A/S Fusion polypeptides with deamidase inhibitor and deamidase domains
EP4242303A1 (en) 2022-03-08 2023-09-13 Novozymes A/S Fusion polypeptides with deamidase inhibitor and deamidase domains
WO2024121357A1 (en) 2022-12-08 2024-06-13 Novozymes A/S Fiber-degrading enzymes for animal feed comprising an oil seed material
WO2024133495A1 (en) 2022-12-21 2024-06-27 Novozymes A/S Microbial proteases for cell detachment
EP4389886A1 (en) 2022-12-22 2024-06-26 Novozymes A/S Lactase enzymes and method of producing a milk-based product

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DK639689D0 (en) * 1989-12-18 1989-12-18 Novo Nordisk As INTRODUCING DNA IN CELLS
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
WO2001000819A1 (en) * 1999-06-25 2001-01-04 Chauhan, Sarita Targeted gene replacements in enteric bacteria using linear dna
EP1297170B1 (en) * 2000-06-23 2016-03-02 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
ATE400648T1 (en) * 2000-08-11 2008-07-15 Genencor Int TRANSFORMATION OF BACILLUS, TRANSFORMANTS AND MUTANT LIBRARIES

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108165516A (en) * 2018-03-22 2018-06-15 江南大学 A kind of method of fermentation of bacillus subtilis production propylhomoserin dehydrogenase
CN108165516B (en) * 2018-03-22 2020-07-07 江南大学 A kind of method of Bacillus subtilis fermentation to produce leucine dehydrogenase

Also Published As

Publication number Publication date
WO2003095658A1 (en) 2003-11-20

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase
TH Corrigenda

Free format text: IN VOL 18, NO 2, PAGE(S) 562 UNDER THE HEADING APPLICATIONS OPI - NAME INDEX UNDER THE NAME NOVOZYMES A/S, APPLICATION NO. 2003223928, UNDER INID (43) CORRECT THE PUBLICATION DATE TO READ 24.11.2003