AU2003223928A1 - Homologous recombination into bacterium for the generation of polynucleotide libraries - Google Patents
Homologous recombination into bacterium for the generation of polynucleotide librariesInfo
- Publication number
- AU2003223928A1 AU2003223928A1 AU2003223928A AU2003223928A AU2003223928A1 AU 2003223928 A1 AU2003223928 A1 AU 2003223928A1 AU 2003223928 A AU2003223928 A AU 2003223928A AU 2003223928 A AU2003223928 A AU 2003223928A AU 2003223928 A1 AU2003223928 A1 AU 2003223928A1
- Authority
- AU
- Australia
- Prior art keywords
- bacterium
- generation
- homologous recombination
- polynucleotide libraries
- libraries
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200200682 | 2002-05-07 | ||
DKPA200200682 | 2002-05-07 | ||
PCT/DK2003/000301 WO2003095658A1 (en) | 2002-05-07 | 2003-05-07 | Homologous recombination into bacterium for the generation of polynucleotide libraries |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2003223928A1 true AU2003223928A1 (en) | 2003-11-11 |
Family
ID=29414623
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2003223928A Abandoned AU2003223928A1 (en) | 2002-05-07 | 2003-05-07 | Homologous recombination into bacterium for the generation of polynucleotide libraries |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU2003223928A1 (en) |
WO (1) | WO2003095658A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108165516A (en) * | 2018-03-22 | 2018-06-15 | 江南大学 | A kind of method of fermentation of bacillus subtilis production propylhomoserin dehydrogenase |
Families Citing this family (60)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2563173A1 (en) * | 2004-03-31 | 2005-10-20 | Novozymes Biopolymer A/S | Methods for producing hyaluronic acid in a bacillus cell |
ES2380105T3 (en) | 2004-06-21 | 2012-05-08 | Novozymes A/S | Nocardiopsis proteases |
US20080038780A1 (en) * | 2006-02-15 | 2008-02-14 | Novozymes Biopolymer A/S | Production of low molecular weight hyaluronic acid |
ES2720369T3 (en) | 2008-06-06 | 2019-07-19 | Procter & Gamble | Detergent composition comprising a variant of a family xyloglucanase 44 |
EP2674488A1 (en) | 2008-06-06 | 2013-12-18 | Novozymes A/S | Variants of a family 44 xyloglucanase |
DE102008052529A1 (en) * | 2008-10-21 | 2010-04-22 | Henkel Ag & Co. Kgaa | Expression-enhanced nucleic acids |
US8409830B2 (en) | 2009-08-21 | 2013-04-02 | Novozymes A/S | Polypeptides having isoamylase activity and methods of use |
EP3550016B1 (en) | 2009-11-06 | 2022-05-04 | Novozymes, Inc. | Composition for saccharification of cellulosic material |
US20120329090A1 (en) | 2009-12-21 | 2012-12-27 | Novozymes A/S | Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells |
EP2539447B1 (en) | 2010-02-25 | 2017-07-26 | Novozymes A/S | Variants of a lysozyme and polynucleotides encoding same |
CN102884182A (en) | 2010-03-03 | 2013-01-16 | 诺维信股份有限公司 | Xylanase variants and polynucleotides encoding same |
EP2647710A1 (en) | 2010-04-09 | 2013-10-09 | Eviagenics S.A. | Method of generating gene mosaics |
EP2616483A1 (en) | 2010-09-16 | 2013-07-24 | Novozymes A/S | Lysozymes |
EP2744900B1 (en) | 2011-08-19 | 2017-07-19 | Novozymes A/S | Polypeptides having protease activity |
US20140274803A1 (en) | 2011-10-12 | 2014-09-18 | Eviagenics S.A. | Method of generating gene mosaics in eukaryotic cells |
ES2667318T3 (en) | 2011-12-28 | 2018-05-10 | Novozymes A/S | Polypeptides with protease activity |
DK2847308T3 (en) | 2012-05-07 | 2017-10-23 | Novozymes As | Polypeptides with xanthan-degrading activity and polynucleotides encoding them |
US20150140165A1 (en) | 2012-06-20 | 2015-05-21 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
WO2014096259A1 (en) | 2012-12-21 | 2014-06-26 | Novozymes A/S | Polypeptides having protease activiy and polynucleotides encoding same |
CN105378079B (en) * | 2013-07-12 | 2019-09-27 | 诺维信公司 | Direct transfer of polynucleotides between genomes |
US10030239B2 (en) | 2013-12-20 | 2018-07-24 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
EP3234143B1 (en) | 2014-12-19 | 2023-06-28 | Novozymes A/S | Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity |
WO2016196202A1 (en) | 2015-05-29 | 2016-12-08 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
US10450539B2 (en) | 2015-06-04 | 2019-10-22 | Novozymes A/S | Use of M4 metalloprotease in wort production |
CN108350441B (en) | 2015-10-07 | 2022-09-27 | 诺维信公司 | Polypeptides |
WO2017064253A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2017089366A1 (en) | 2015-11-24 | 2017-06-01 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
EP3408386A1 (en) | 2016-01-29 | 2018-12-05 | Novozymes A/S | Beta-glucanase variants and polynucleotides encoding same |
US10947520B2 (en) | 2016-05-24 | 2021-03-16 | Novozymes A/S | Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity |
CN109153981A (en) | 2016-05-24 | 2019-01-04 | 诺维信公司 | Polypeptide with alpha-galactosidase activity and the polynucleotides for encoding it |
CN109196098A (en) | 2016-05-24 | 2019-01-11 | 诺维信公司 | Composition comprising the polypeptide with galactanase activity and the polypeptide with betagalactosidase activity |
KR102507692B1 (en) | 2016-08-24 | 2023-03-09 | 헨켈 아게 운트 코. 카게아아 | Detergent composition comprising GH9 endoglucanase variant I |
WO2018037062A1 (en) | 2016-08-24 | 2018-03-01 | Novozymes A/S | Gh9 endoglucanase variants and polynucleotides encoding same |
CN110072997A (en) | 2016-11-23 | 2019-07-30 | 诺维信公司 | Polypeptide with proteinase activity and the polynucleotides for encoding it |
US11149233B2 (en) | 2017-03-31 | 2021-10-19 | Novozymes A/S | Polypeptides having RNase activity |
EP3601549A1 (en) | 2017-03-31 | 2020-02-05 | Novozymes A/S | Polypeptides having dnase activity |
WO2019038059A1 (en) | 2017-08-24 | 2019-02-28 | Henkel Ag & Co. Kgaa | Detergent compositions comprising gh9 endoglucanase variants ii |
CN111278971A (en) | 2017-08-24 | 2020-06-12 | 诺维信公司 | GH9 endoglucanase variants and polynucleotides encoding same |
AR112955A1 (en) | 2017-09-01 | 2020-01-08 | Novozymes As | ANIMAL FEED ADDITIVES INCLUDING POLYPEPTIDES HAVING PROTEASE ACTIVITY AND USES OF THE SAME |
US11414814B2 (en) | 2017-09-22 | 2022-08-16 | Novozymes A/S | Polypeptides |
EP3692147A1 (en) | 2017-10-02 | 2020-08-12 | Novozymes A/S | Polypeptides having mannanase activity and polynucleotides encoding same |
EP3692148A1 (en) | 2017-10-02 | 2020-08-12 | Novozymes A/S | Polypeptides having mannanase activity and polynucleotides encoding same |
US20200248159A1 (en) | 2017-10-04 | 2020-08-06 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2019081515A1 (en) | 2017-10-24 | 2019-05-02 | Novozymes A/S | Compositions comprising polypeptides having mannanase activity |
WO2019096903A1 (en) | 2017-11-20 | 2019-05-23 | Novozymes A/S | New galactanases (ec 3.2.1.89) for use in soy processing |
US20210102184A1 (en) | 2018-02-23 | 2021-04-08 | Henkel Ag & Co. Kgaa | Detergent composition comprising xanthan lyase and endoglucanase variants |
US11473076B2 (en) | 2018-08-31 | 2022-10-18 | Novozymes A/S | Animal feed additives and compositions comprising an S8 serine protease |
US11993762B2 (en) | 2018-10-03 | 2024-05-28 | Novozymes A/S | Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same |
EP3947619A1 (en) | 2019-04-03 | 2022-02-09 | Novozymes A/S | Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions |
EP4077656A2 (en) | 2019-12-20 | 2022-10-26 | Novozymes A/S | Polypeptides having proteolytic activity and use thereof |
EP4103709A2 (en) | 2020-02-10 | 2022-12-21 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
WO2021163015A1 (en) | 2020-02-10 | 2021-08-19 | Novozymes A/S | Process for producing ethanol from raw starch using alpha-amylase variants |
US20230142991A1 (en) | 2020-02-10 | 2023-05-11 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2022161914A1 (en) | 2021-01-26 | 2022-08-04 | Basf Se | High temperature fermentation process and microorganisms |
AU2022422537A1 (en) | 2021-12-22 | 2024-06-06 | Novozymes A/S | Method of producing a milk-based product |
AU2023231428A1 (en) | 2022-03-08 | 2024-07-11 | Novozymes A/S | Fusion polypeptides with deamidase inhibitor and deamidase domains |
EP4242303A1 (en) | 2022-03-08 | 2023-09-13 | Novozymes A/S | Fusion polypeptides with deamidase inhibitor and deamidase domains |
WO2024121357A1 (en) | 2022-12-08 | 2024-06-13 | Novozymes A/S | Fiber-degrading enzymes for animal feed comprising an oil seed material |
WO2024133495A1 (en) | 2022-12-21 | 2024-06-27 | Novozymes A/S | Microbial proteases for cell detachment |
EP4389886A1 (en) | 2022-12-22 | 2024-06-26 | Novozymes A/S | Lactase enzymes and method of producing a milk-based product |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK639689D0 (en) * | 1989-12-18 | 1989-12-18 | Novo Nordisk As | INTRODUCING DNA IN CELLS |
US5955310A (en) * | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
WO2001000819A1 (en) * | 1999-06-25 | 2001-01-04 | Chauhan, Sarita | Targeted gene replacements in enteric bacteria using linear dna |
EP1297170B1 (en) * | 2000-06-23 | 2016-03-02 | Novozymes A/S | Method for stable chromosomal multi-copy integration of genes |
ATE400648T1 (en) * | 2000-08-11 | 2008-07-15 | Genencor Int | TRANSFORMATION OF BACILLUS, TRANSFORMANTS AND MUTANT LIBRARIES |
-
2003
- 2003-05-07 WO PCT/DK2003/000301 patent/WO2003095658A1/en not_active Application Discontinuation
- 2003-05-07 AU AU2003223928A patent/AU2003223928A1/en not_active Abandoned
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108165516A (en) * | 2018-03-22 | 2018-06-15 | 江南大学 | A kind of method of fermentation of bacillus subtilis production propylhomoserin dehydrogenase |
CN108165516B (en) * | 2018-03-22 | 2020-07-07 | 江南大学 | A kind of method of Bacillus subtilis fermentation to produce leucine dehydrogenase |
Also Published As
Publication number | Publication date |
---|---|
WO2003095658A1 (en) | 2003-11-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK6 | Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase | ||
TH | Corrigenda |
Free format text: IN VOL 18, NO 2, PAGE(S) 562 UNDER THE HEADING APPLICATIONS OPI - NAME INDEX UNDER THE NAME NOVOZYMES A/S, APPLICATION NO. 2003223928, UNDER INID (43) CORRECT THE PUBLICATION DATE TO READ 24.11.2003 |