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AU2003223928A1 - Homologous recombination into bacterium for the generation of polynucleotide libraries - Google Patents

Homologous recombination into bacterium for the generation of polynucleotide libraries

Info

Publication number
AU2003223928A1
AU2003223928A1 AU2003223928A AU2003223928A AU2003223928A1 AU 2003223928 A1 AU2003223928 A1 AU 2003223928A1 AU 2003223928 A AU2003223928 A AU 2003223928A AU 2003223928 A AU2003223928 A AU 2003223928A AU 2003223928 A1 AU2003223928 A1 AU 2003223928A1
Authority
AU
Australia
Prior art keywords
bacterium
generation
homologous recombination
polynucleotide libraries
libraries
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003223928A
Inventor
Mads Eskelund Bjornvad
Peter Kamp Hansen
Per Lina Jorgensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of AU2003223928A1 publication Critical patent/AU2003223928A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003223928A 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries Abandoned AU2003223928A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200200682 2002-05-07
DKPA200200682 2002-05-07
PCT/DK2003/000301 WO2003095658A1 (en) 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries

Publications (1)

Publication Number Publication Date
AU2003223928A1 true AU2003223928A1 (en) 2003-11-11

Family

ID=29414623

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003223928A Abandoned AU2003223928A1 (en) 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries

Country Status (2)

Country Link
AU (1) AU2003223928A1 (en)
WO (1) WO2003095658A1 (en)

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CN108165516A (en) * 2018-03-22 2018-06-15 江南大学 A kind of method of fermentation of bacillus subtilis production propylhomoserin dehydrogenase

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CA2591858C (en) 2004-06-21 2015-05-05 Novozymes A/S Proteases derived from norcardiopsis
US20080038780A1 (en) * 2006-02-15 2008-02-14 Novozymes Biopolymer A/S Production of low molecular weight hyaluronic acid
EP2300588B1 (en) 2008-06-06 2019-02-06 The Procter and Gamble Company Detergent composition comprising a variant of a family 44 xyloglucanase
EP2905333B1 (en) 2008-06-06 2017-08-09 Novozymes A/S Variants of a family 44 xyloglucanase
DE102008052529A1 (en) * 2008-10-21 2010-04-22 Henkel Ag & Co. Kgaa Expression-enhanced nucleic acids
EP2467476A1 (en) 2009-08-21 2012-06-27 Novozymes A/S Polypeptides having isoamylase activity and polynucleotides encoding same
DK2496694T3 (en) 2009-11-06 2017-06-06 Novozymes Inc COMPOSITIONS FOR SACCHARIFYING CELLULOS MATERIAL
US20120329090A1 (en) 2009-12-21 2012-12-27 Novozymes A/S Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells
CN102858968B (en) 2010-02-25 2015-07-01 诺维信公司 Variants of a lysozyme and polynucleotides encoding same
CA2791353A1 (en) 2010-03-03 2011-09-09 Novozymes, Inc. Xylanase variants and polynucleotides encoding same
HRP20140079T1 (en) 2010-04-09 2014-02-28 Eviagenics S.A. Method of generating gene mosaics
WO2012035103A1 (en) 2010-09-16 2012-03-22 Novozymes A/S Lysozymes
US20140295027A1 (en) 2011-08-19 2014-10-02 Novozymes A/S Polypeptides Having Protease Activity
CN104105793A (en) 2011-10-12 2014-10-15 埃微杰尼克斯有限公司 Method of generating gene mosaics in eukaryotic cells
WO2013098185A1 (en) 2011-12-28 2013-07-04 Novozymes A/S Polypeptides having protease activity
ES2643216T3 (en) 2012-05-07 2017-11-21 Novozymes A/S Polypeptides with degradation activity of xanthan and polynucleotides encoding it
AU2013279440B2 (en) 2012-06-20 2016-10-06 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
EP2934177B1 (en) 2012-12-21 2017-10-25 Novozymes A/S Polypeptides having protease activiy and polynucleotides encoding same
CN105378079B (en) * 2013-07-12 2019-09-27 诺维信公司 Direct transfer of polynucleotides between genomes
EP3453757B1 (en) 2013-12-20 2020-06-17 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CN107208080B (en) 2014-12-19 2022-03-25 诺维信公司 Compositions comprising a polypeptide having xylanase activity and a polypeptide having arabinofuranosidase activity
WO2016196202A1 (en) 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
US10450539B2 (en) 2015-06-04 2019-10-22 Novozymes A/S Use of M4 metalloprotease in wort production
EP3359658A2 (en) 2015-10-07 2018-08-15 Novozymes A/S Polypeptides
WO2017064253A1 (en) 2015-10-14 2017-04-20 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
EP3380608A1 (en) 2015-11-24 2018-10-03 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
MX2018008051A (en) 2016-01-29 2018-08-23 Novozymes As Beta-glucanase variants and polynucleotides encoding same.
EP4446410A3 (en) 2016-05-24 2025-01-01 Novozymes A/S Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity
EP3464581A1 (en) 2016-05-24 2019-04-10 Novozymes A/S Polypeptides having alpha-galactosidase activity and polynucleotides encoding same
AU2017270231A1 (en) 2016-05-24 2018-11-15 Novozymes A/S Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity
WO2018037065A1 (en) 2016-08-24 2018-03-01 Henkel Ag & Co. Kgaa Detergent composition comprising gh9 endoglucanase variants i
US11072765B2 (en) 2016-08-24 2021-07-27 Novozymes A/S GH9 endoglucanase variants and polynucleotides encoding same
EP3545086A1 (en) 2016-11-23 2019-10-02 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
WO2018178061A1 (en) 2017-03-31 2018-10-04 Novozymes A/S Polypeptides having rnase activity
US11053483B2 (en) 2017-03-31 2021-07-06 Novozymes A/S Polypeptides having DNase activity
WO2019038059A1 (en) 2017-08-24 2019-02-28 Henkel Ag & Co. Kgaa Detergent compositions comprising gh9 endoglucanase variants ii
WO2019038058A1 (en) 2017-08-24 2019-02-28 Novozymes A/S Gh9 endoglucanase variants and polynucleotides encoding same
JP7515396B2 (en) 2017-09-01 2024-07-12 ノボザイムス アクティーゼルスカブ Animal feed additive containing polypeptide having protease activity and use thereof
US11414814B2 (en) 2017-09-22 2022-08-16 Novozymes A/S Polypeptides
EP3692147A1 (en) 2017-10-02 2020-08-12 Novozymes A/S Polypeptides having mannanase activity and polynucleotides encoding same
US11732221B2 (en) 2017-10-02 2023-08-22 Novozymes A/S Polypeptides having mannanase activity and polynucleotides encoding same
US20200248159A1 (en) 2017-10-04 2020-08-06 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
US11866748B2 (en) 2017-10-24 2024-01-09 Novozymes A/S Compositions comprising polypeptides having mannanase activity
WO2019096903A1 (en) 2017-11-20 2019-05-23 Novozymes A/S New galactanases (ec 3.2.1.89) for use in soy processing
EP3755793A1 (en) 2018-02-23 2020-12-30 Henkel AG & Co. KGaA Detergent composition comprising xanthan lyase and endoglucanase variants
EP3843552A1 (en) 2018-08-31 2021-07-07 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
EP3861008A1 (en) 2018-10-03 2021-08-11 Novozymes A/S Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same
CN113785039B (en) 2019-04-03 2024-06-18 诺维信公司 Polypeptides having beta-glucanase activity, polynucleotides encoding the same, and their use in cleaning and detergent compositions
US20220411773A1 (en) 2019-12-20 2022-12-29 Novozymes A/S Polypeptides having proteolytic activity and use thereof
EP4103709A2 (en) 2020-02-10 2022-12-21 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
BR112022015805A2 (en) 2020-02-10 2022-10-11 Novozymes As ALPHA-AMYLASE VARIANT OF AN ALPHA-AMYLASE PROGENITOR, ISOLATED POLYNUCLEOTIDE, NUCLEIC ACID CONSTRUCT OR EXPRESSION VECTOR, RECOMBINANT HOST CELL, COMPOSITION, WHOLE BROTH FORMULATION OR CELL CULTURE COMPOSITION, USE OF A VARIANT ALPHA-AMYLASE, PROCESS OF PRODUCTION OF A FERMENTATION PRODUCT FROM MATERIAL CONTAINING STARCH AND A SYRUP PRODUCT, AND, TRANSGENIC PLANT, PLANT PART OR PLANT CELL
US20230235367A1 (en) 2020-02-10 2023-07-27 Novozymes A/S Process for producing ethanol from raw starch using alpha-amylase variants
WO2022161914A1 (en) 2021-01-26 2022-08-04 Basf Se High temperature fermentation process and microorganisms
EP4451889A1 (en) 2021-12-22 2024-10-30 Novozymes A/S Method of producing a milk-based product
EP4242303A1 (en) 2022-03-08 2023-09-13 Novozymes A/S Fusion polypeptides with deamidase inhibitor and deamidase domains
EP4490286A1 (en) 2022-03-08 2025-01-15 Novozymes A/S Fusion polypeptides with deamidase inhibitor and deamidase domains
CN120112175A (en) 2022-12-08 2025-06-06 诺维信公司 Fiber degrading enzymes for animal feeds containing oilseed material
CN120476196A (en) 2022-12-21 2025-08-12 诺维信公司 Microbial proteases for cell separation
EP4389886A1 (en) 2022-12-22 2024-06-26 Novozymes A/S Lactase enzymes and method of producing a milk-based product
WO2025196175A1 (en) 2024-03-22 2025-09-25 Novozymes A/S Oral care compositions comprising dnases

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DK639689D0 (en) * 1989-12-18 1989-12-18 Novo Nordisk As INTRODUCING DNA IN CELLS
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
WO2001000819A1 (en) * 1999-06-25 2001-01-04 Chauhan, Sarita Targeted gene replacements in enteric bacteria using linear dna
WO2002000907A1 (en) * 2000-06-23 2002-01-03 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
AU2001279254A1 (en) * 2000-08-11 2002-02-25 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108165516A (en) * 2018-03-22 2018-06-15 江南大学 A kind of method of fermentation of bacillus subtilis production propylhomoserin dehydrogenase
CN108165516B (en) * 2018-03-22 2020-07-07 江南大学 A kind of method of Bacillus subtilis fermentation to produce leucine dehydrogenase

Also Published As

Publication number Publication date
WO2003095658A1 (en) 2003-11-20

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase
TH Corrigenda

Free format text: IN VOL 18, NO 2, PAGE(S) 562 UNDER THE HEADING APPLICATIONS OPI - NAME INDEX UNDER THE NAME NOVOZYMES A/S, APPLICATION NO. 2003223928, UNDER INID (43) CORRECT THE PUBLICATION DATE TO READ 24.11.2003