DK175738B1 - Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer - Google Patents

Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer Download PDF

Info

Publication number: DK175738B1
Authority: DK; Denmark
Prior art keywords: bacillus; host cell; protease; dna; transformed
Prior art date: 1987-02-27

Application number

DK198805939A

Other languages

Danish (da)

English (en)

Other versions

DK593988A (da

DK593988D0 (da

Inventor

Leonardus Johannes S Mulleners

Christiaan Albertus Ge Eekelen

Johannes Cornelis Van Der Laan

Original Assignee

Genencor Int

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-02-27

Filing date

1988-10-26

Publication date

2005-02-07

1988-10-26 Application filed by Genencor Int filed Critical Genencor Int

1988-10-26 Publication of DK593988A publication Critical patent/DK593988A/da

1988-10-26 Publication of DK593988D0 publication Critical patent/DK593988D0/da

2004-08-20 Priority to DK200401262A priority Critical patent/DK175852B1/da

2005-02-07 Application granted granted Critical

2005-02-07 Publication of DK175738B1 publication Critical patent/DK175738B1/da

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Mycology (AREA)
Tropical Medicine & Parasitology (AREA)
Virology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Peptides Or Proteins (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

DK198805939A 1987-02-27 1988-10-26 Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer DK175738B1 (da)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
DK200401262A DK175852B1 (da)	1987-02-27	2004-08-20	Transformation af alkalofile Bacillus-stammer

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
EP87200358		1987-02-27
EP87200358		1987-02-27
NL8800007		1988-02-26
PCT/NL1988/000007 WO1988006624A2 (en)	1987-02-27	1988-02-26	Molecular cloning and expression of genes encoding proteolytic enzymes

Publications (3)

Publication Number	Publication Date
DK593988A DK593988A (da)	1988-10-26
DK593988D0 DK593988D0 (da)	1988-10-26
DK175738B1 true DK175738B1 (da)	2005-02-07

Family

ID=8197584

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
DK198805939A DK175738B1 (da)	1987-02-27	1988-10-26	Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer
DK200401262A DK175852B1 (da)	1987-02-27	2004-08-20	Transformation af alkalofile Bacillus-stammer

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
DK200401262A DK175852B1 (da)	1987-02-27	2004-08-20	Transformation af alkalofile Bacillus-stammer

Country Status (19)

Country	Link
US (1)	US5217878A (fi)
JP (1)	JP3026567B2 (fi)
KR (1)	KR0127560B1 (fi)
CN (1)	CN88101681A (fi)
AT (2)	ATE181110T1 (fi)
AU (1)	AU620026B2 (fi)
BG (1)	BG49049A3 (fi)
BR (1)	BR8805647A (fi)
CA (1)	CA1339100C (fi)
DE (2)	DE3856339T2 (fi)
DK (2)	DK175738B1 (fi)
FI (3)	FI105483B (fi)
HU (1)	HU213220B (fi)
LT (1)	LT4000B (fi)
LV (1)	LV10307B (fi)
NO (1)	NO884423L (fi)
PT (1)	PT86864B (fi)
RU (1)	RU2023723C1 (fi)
WO (1)	WO1988006624A2 (fi)

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US5665587A (en) *	1989-06-26	1997-09-09	Novo Nordisk A/S	Modified subtilisins and detergent compositions containing same
JP3046344B2 (ja) *	1990-10-24	2000-05-29	塩野義製薬株式会社	新規プロテアーゼ
US5482849A (en) *	1990-12-21	1996-01-09	Novo Nordisk A/S	Subtilisin mutants
DK58391D0 (da) *	1991-04-03	1991-04-03	Novo Nordisk As	Hidtil ukendte proteaser
EP0571014B1 (en) *	1992-05-18	2004-03-31	Genencor International, Inc.	Bacteria producing alkaline proteases, and production of these alkaline proteases
US6440717B1 (en) *	1993-09-15	2002-08-27	The Procter & Gamble Company	BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) *	1993-09-15	2002-08-20	The Procter & Gamble Company	BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) *	1994-05-02	2003-07-29	Procter & Gamble Company	Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5831004A (en)	1994-10-27	1998-11-03	Affymax Technologies N.V.	Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use
US5646044A (en) *	1995-03-02	1997-07-08	Henkel Kommanditgesellschaft Auf Aktien	Expression systems for the production of target proteins in bacillus
US6455295B1 (en) *	1995-03-08	2002-09-24	The Procter & Gamble Company	Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) *	1995-03-09	2002-11-05	Procter & Gamble Company	Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) *	1995-03-09	1996-07-23	Procter & Gamble	Proteinase k variants having decreased adsorption and increased hydrolysis
US6300116B1 (en)	1996-11-04	2001-10-09	Novozymes A/S	Modified protease having improved autoproteolytic stability
US5766871A (en) *	1996-11-27	1998-06-16	Food Industry Research And Development Institute	Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US6485957B1 (en)	1999-04-30	2002-11-26	Ortho-Mcneil Pharmaceutical, Inc.	DNA encoding the human serine protease EOS
US6420157B1 (en) *	1999-04-30	2002-07-16	Ortho-Mcneil Pharmaceutical, Inc.	Zymogen activation system
US6458564B1 (en)	1999-08-31	2002-10-01	Ortho-Mcneil Pharmaceutical, Inc.	DNA encoding the human serine protease T
US6426199B1 (en)	1999-08-31	2002-07-30	Ortho-Mcneil Pharmaceutical, Inc.	Dna
CN1312370A (zh) *	2000-03-07	2001-09-12	上海博德基因开发有限公司	一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸
US6777218B1 (en) *	2000-03-14	2004-08-17	Novozymes A/S	Subtilase enzymes having an improved wash performance on egg stains
AU4981101A (en) *	2000-04-03	2001-10-15	Maxygen Inc	Subtilisin variants
JP4680512B2 (ja)	2002-03-29	2011-05-11	ジェネンコー・インターナショナル・インク	バチルスにおけるタンパク質発現の増大
WO2004101760A2 (en) *	2003-05-12	2004-11-25	Genencor International, Inc.	Novel lipolytic enzyme elip
EP1625208A4 (en) *	2003-05-12	2006-10-18	Genencor Int	NEW LIPOLYTIC ENZYME LIP2
WO2004101763A2 (en)	2003-05-12	2004-11-25	Genencor International, Inc.	Novel lipolytic enzyme lip1
EP1667530A4 (en)	2003-09-19	2011-05-25	Ics Holdings Inc	MANUFACTURE OF A COURABLE PRODUCT OF DOUGH
US7985569B2 (en)	2003-11-19	2011-07-26	Danisco Us Inc.	Cellulomonas 69B4 serine protease variants
KR101482015B1 (ko) *	2003-11-19	2015-01-23	다니스코 유에스 인크.	세린 프로테아제, 세린 효소들을 인코딩하는 핵산 및 이를편입시킨 벡터 및 숙주 세포
EP1735339A2 (en) *	2004-04-09	2006-12-27	Genencor International, Inc.	Pcka modifications and enhanced protein expression in bacillus
EP1766002B1 (en) *	2004-06-21	2015-11-18	Novozymes A/S	Stably maintained multiple copies of at least two orf in the same orientation
US7202074B2 (en) *	2004-07-22	2007-04-10	University Of Chile	Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
WO2008112258A2 (en)	2007-03-12	2008-09-18	Danisco Us Inc.	Modified proteases
EP2460823B1 (en)	2007-05-10	2014-02-26	Danisco US Inc.	A modified secretion system to increase expression of polypeptides in bacteria
EP2173873B1 (en)	2007-08-06	2015-11-18	University of Chile	Protein and dna sequence encoding a cold adapted subtilisin-like activity
JP5629584B2 (ja)	2007-12-21	2014-11-19	ダニスコ・ユーエス・インク	バチルス内での促進されたタンパク質生成
FI123122B (fi)	2009-02-16	2012-11-15	Optogear Oy	Laitteisto lasimateriaalin valmistamiseksi
CA2759695C (en) *	2009-04-24	2018-05-01	Danisco Us Inc.	Proteases with modified pro regions
AR076941A1 (es)	2009-06-11	2011-07-20	Danisco Us Inc	Cepa de bacillus para una mayor produccion de proteina
MX350391B (es) *	2011-09-22	2017-09-06	Novozymes As	Polipeptidos que poseen actividad proteasa y polinucleotidos que los codifican.
CN103013960A (zh) *	2012-12-21	2013-04-03	青岛蔚蓝生物集团有限公司	一种碱性蛋白酶及其重组表达工程菌
DK3089991T3 (da)	2013-12-31	2019-12-02	Danisco Us Inc	Forbedret proteinekspression
EP3233893B1 (en)	2014-12-16	2021-01-20	Danisco US Inc.	Enhanced protein expression
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GB1234445A (fi)	1967-10-03	1971-06-03
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1988
- 1988-02-26 WO PCT/NL1988/000007 patent/WO1988006624A2/en active IP Right Grant
- 1988-02-26 AU AU13980/88A patent/AU620026B2/en not_active Expired
- 1988-02-26 KR KR1019880701347A patent/KR0127560B1/ko not_active IP Right Cessation
- 1988-02-26 PT PT86864A patent/PT86864B/pt not_active IP Right Cessation
- 1988-02-26 HU HU881832A patent/HU213220B/hu not_active IP Right Cessation
- 1988-02-26 BR BR888805647A patent/BR8805647A/pt not_active IP Right Cessation
- 1988-02-26 JP JP63502472A patent/JP3026567B2/ja not_active Expired - Lifetime
- 1988-02-27 CN CN198888101681A patent/CN88101681A/zh active Pending
- 1988-02-29 CA CA000560126A patent/CA1339100C/en not_active Expired - Lifetime
- 1988-02-29 AT AT91203297T patent/ATE181110T1/de not_active IP Right Cessation
- 1988-02-29 DE DE3856339T patent/DE3856339T2/de not_active Expired - Lifetime
- 1988-02-29 US US07/162,184 patent/US5217878A/en not_active Expired - Lifetime
- 1988-02-29 DE DE3856331T patent/DE3856331T2/de not_active Expired - Lifetime
- 1988-02-29 AT AT88200377T patent/ATE180016T1/de not_active IP Right Cessation
- 1988-10-05 NO NO884423A patent/NO884423L/no unknown
- 1988-10-24 FI FI884904A patent/FI105483B/fi active IP Right Grant
- 1988-10-25 BG BG085831A patent/BG49049A3/xx unknown
- 1988-10-26 DK DK198805939A patent/DK175738B1/da not_active IP Right Cessation
- 1988-10-26 RU SU884356848A patent/RU2023723C1/ru active
1992
- 1992-11-27 FI FI925413A patent/FI106726B/fi active
1993
- 1993-12-29 LV LVP-93-1390A patent/LV10307B/en unknown
1994
- 1994-01-28 LT LTIP1820A patent/LT4000B/lt not_active IP Right Cessation
2000
- 2000-05-15 FI FI20001152A patent/FI119028B/fi not_active IP Right Cessation
2004
- 2004-08-20 DK DK200401262A patent/DK175852B1/da not_active IP Right Cessation

Also Published As

Publication number	Publication date
ATE180016T1 (de)	1999-05-15
WO1988006624A3 (en)	1988-11-17
FI105483B (fi)	2000-08-31
DE3856339T2 (de)	1999-11-04
CN88101681A (zh)	1988-11-09
HU213220B (en)	1997-03-28
FI925413A0 (fi)	1992-11-27
US5217878A (en)	1993-06-08
BR8805647A (pt)	1989-10-31
AU620026B2 (en)	1992-02-13
LTIP1820A (en)	1995-08-25
CA1339100C (en)	1997-07-29
DE3856331T2 (de)	1999-10-14
DE3856331D1 (de)	1999-06-17
KR890700665A (ko)	1989-04-26
PT86864B (pt)	1992-05-29
BG49049A3 (en)	1991-07-15
DK593988A (da)	1988-10-26
FI119028B (fi)	2008-06-30
AU1398088A (en)	1988-09-26
LV10307A (lv)	1994-10-20
FI925413A (fi)	1992-11-27
FI884904A (fi)	1988-10-24
WO1988006624A2 (en)	1988-09-07
JP3026567B2 (ja)	2000-03-27
LT4000B (en)	1996-06-25
NO884423D0 (no)	1988-10-05
LV10307B (en)	1995-08-20
RU2023723C1 (ru)	1994-11-30
FI106726B (fi)	2001-03-30
FI884904A0 (fi)	1988-10-24
PT86864A (pt)	1988-03-01
JPH01502876A (ja)	1989-10-05
ATE181110T1 (de)	1999-06-15
DE3856339D1 (de)	1999-07-15
DK200401262A (da)	2004-08-20
DK175852B1 (da)	2005-04-04
KR0127560B1 (ko)	1997-12-29
NO884423L (no)	1988-10-05
FI20001152A (fi)	2000-05-15
DK593988D0 (da)	1988-10-26

Publication	Publication Date	Title
DK175738B1 (da)	2005-02-07	Molekylær kloning og ekspression af gener, der koder for proteolytiske enzymer
EP0283075B1 (en)	1999-05-12	Molecular cloning and expression of genes encoding proteolytic enzymes
EP0284126B1 (en)	1993-08-11	Stable gene amplification in prokaryotic chromosomal dna
DK169996B1 (da)	1995-04-24	Fremgangsmåde til effektiv indføring af DNA i en industriel Bacillus-stammevært, fremgangsmåde til fremstilling af et polypeptid i en sådan vært, plasmid, genetisk manipuleret industriel Bacillus-mikroorganisme og anvendelse af en sådan mikroorganisme
EP0127328A2 (en)	1984-12-05	The use of chromosomal integration to stabilize heterologous genes
US5716807A (en)	1998-02-10	Transformed industrial bacillus strains and methods for making and using them
JP3154720B2 (ja)	2001-04-09	誘導性発現ベクター
EP0635061A1 (en)	1995-01-25	A method of limiting the survival of genetically engineered microorganisms in their environment
EP0303668A1 (en)	1989-02-22	Stable expression vectors
JPH06303986A (ja)	1994-11-01	形質転換した原核宿主細胞の製法
JPH0783716B2 (ja)	1995-09-13	アルカリプロテアーゼをコードする遺伝子系

Legal Events

Date	Code	Title	Description
2008-03-17	PUP	Patent expired