[go: up one dir, main page]

ES8802256A1 - Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos biologicos. - Google Patents

Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos biologicos.

Info

Publication number
ES8802256A1
ES8802256A1 ES551760A ES551760A ES8802256A1 ES 8802256 A1 ES8802256 A1 ES 8802256A1 ES 551760 A ES551760 A ES 551760A ES 551760 A ES551760 A ES 551760A ES 8802256 A1 ES8802256 A1 ES 8802256A1
Authority
ES
Spain
Prior art keywords
enzyme
immunoprocess
determination
antibodies
antigens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES551760A
Other languages
English (en)
Other versions
ES551760A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Italia SpA
Original Assignee
Boehringer Biochemia Robin SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Biochemia Robin SpA filed Critical Boehringer Biochemia Robin SpA
Publication of ES8802256A1 publication Critical patent/ES8802256A1/es
Publication of ES551760A0 publication Critical patent/ES551760A0/es
Expired legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/538Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54386Analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54386Analytical elements
    • G01N33/54387Immunochromatographic test strips
    • G01N33/54391Immunochromatographic test strips based on vertical flow

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

UN METODO Y UN DISPOSITIVO PARA LA DETERMINACION DE PARAMETROS CLINICOS EN LIQUIDOS BIOLOGICOS MEDIANTE INMUNOPROCESOS ENZIMATICOS, DONDE EL LIQUIDO BIOLOGICO SE PONE EN CONTACTO SUCESIVAMENTE CON: A) ANTICUERPOS O ANTOGENOS MARCADOS POR ENZIMAS, QUE SON ABSORBIDOS SOBRE UN SOPORTE SOLIDO DE FORMA REVERSIBLE; B) ANTICUERPOS, ANTIGENOS, ANTI-ANTICUERPOS O COMPLEJOS DE ANTIGENO-ANTICUERPO QUE SON INMOVILIZADOS SOBRE UN SOPORTE SOLIDO EN CANTIDAD ESTEQUIOMETRICAMENTE EQUIVALENTE A LOS ANTICUERPOS O ANTIGENOS MARCADOS CON ENZIMAS; C) UNO O MAS SUSTRATOS CROMOGENICOS CAPACES DE PRODUCIR REACCIONES DEMOSTRATIVAS SON LOS ENZIMAS CONJUGADOS AL ANTIGENO O AL ANTICUERPO. DE APLICACION PARA EL ANALISIS DE SUSTANCIAS BIOLOGICAS (ORINA, PLASMA, SANGRE, EXUDADOS, EXTRACTOS TISULARES, ETC.) UTILIZANDO CROMATOGRAFIA DE AFINIDAD.
ES551760A 1985-02-08 1986-02-07 Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos biologicos. Expired ES8802256A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT19443/85A IT1200382B (it) 1985-02-08 1985-02-08 Sistema di rilevazione e/o dosaggio di parametri clinici per via immuno enzimatica

Publications (2)

Publication Number Publication Date
ES8802256A1 true ES8802256A1 (es) 1988-04-16
ES551760A0 ES551760A0 (es) 1988-04-16

Family

ID=11158028

Family Applications (5)

Application Number Title Priority Date Filing Date
ES551760A Expired ES8802256A1 (es) 1985-02-08 1986-02-07 Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos biologicos.
ES557662A Expired ES8900067A1 (es) 1985-02-08 1987-08-13 Un metodo y su correspondiente dispositivo para la determinacion de anticuerpos en liquidos biologicos.
ES557803A Expired ES8802259A1 (es) 1985-02-08 1988-01-15 Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos bilogicos
ES557802A Expired ES8802258A1 (es) 1985-02-08 1988-01-15 Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos biologicos
ES557818A Expired ES8802260A1 (es) 1985-02-08 1988-02-01 Un metodo y su correspondiente dispositivo para la determinacion de uno o mas anticuerpos simultaneamente en un liquido biologico

Family Applications After (4)

Application Number Title Priority Date Filing Date
ES557662A Expired ES8900067A1 (es) 1985-02-08 1987-08-13 Un metodo y su correspondiente dispositivo para la determinacion de anticuerpos en liquidos biologicos.
ES557803A Expired ES8802259A1 (es) 1985-02-08 1988-01-15 Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos bilogicos
ES557802A Expired ES8802258A1 (es) 1985-02-08 1988-01-15 Un metodo cromatografico de afinidad para la determinacion de antigenos en liquidos biologicos
ES557818A Expired ES8802260A1 (es) 1985-02-08 1988-02-01 Un metodo y su correspondiente dispositivo para la determinacion de uno o mas anticuerpos simultaneamente en un liquido biologico

Country Status (12)

Country Link
EP (1) EP0243370A1 (es)
JP (1) JPS62501989A (es)
AU (1) AU5511886A (es)
DD (1) DD245727A5 (es)
ES (5) ES8802256A1 (es)
FI (1) FI85194C (es)
HU (1) HUT56632A (es)
IT (1) IT1200382B (es)
MW (1) MW5287A1 (es)
OA (1) OA08640A (es)
RO (1) RO100056A2 (es)
WO (1) WO1986004683A1 (es)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4806311A (en) * 1985-08-28 1989-02-21 Miles Inc. Multizone analytical element having labeled reagent concentration zone
US4959305A (en) * 1986-06-18 1990-09-25 Miles Inc. Reversible immobilization of assay reagents in a multizone test device
CA1289872C (en) * 1986-06-18 1991-10-01 David L. Woodrum Reversible immobilization of assay reagents in a multizone test device
ES2004066A6 (es) * 1987-01-21 1988-12-01 Euro Fassel Aktiebolag Metodo para analisis cualitativo y-o cuantitativo de antigenos,anticuerpos yno microorganismos u otras celulas y su correspondiente equipo
AU592174B2 (en) * 1987-02-17 1990-01-04 Genesis Labs, Inc. Immunoassay test strip
AU586552B2 (en) * 1987-02-25 1989-07-13 Genesis Labs, Inc. Dry test strip for devices using oxygen demanding detection system
DE3856421T2 (de) * 1987-04-27 2000-12-14 Unilever Nv Spezifische Bindungstestverfahren
JP2599192B2 (ja) * 1987-04-29 1997-04-09 セルテク セラピューティクスリミティド 結合アッセイ装置
US5641639A (en) * 1987-04-29 1997-06-24 Celltech Therapeutics Limited Binding assay device
IT1223295B (it) * 1987-08-14 1990-09-19 Boehringer Biochemia Srl Dispositivo e metodo immunodiagnostico
FR2621393B1 (fr) * 1987-10-05 1991-12-13 Toledano Jacques Dispositif de detection immunoenzymatique de substances a partir d'une goutte de sang ou de liquide provenant d'un quelconque milieu biologique
US5334513A (en) * 1988-05-17 1994-08-02 Syntex (U.S.A.) Inc. Method for immunochromatographic analysis
US5039607A (en) * 1988-05-17 1991-08-13 Syntex (U.S.A.) Inc. Method for immunochromatographic analysis
US5338513A (en) * 1988-07-30 1994-08-16 Boehringer Mannheim Gmbh Test carrier for the analytical determination of a component of a liquid sample
FR2634891B1 (fr) * 1988-08-01 1994-05-06 Biotrol Laboratoires Procede et dispositif pour la determination qualitative et/ou quantitative d'un ligand dans un fluide
US5079174A (en) * 1988-12-08 1992-01-07 Boehringer Mannheim Corporation Apparatus for sequential determination of an analyte in a fluid sample
DE3842702A1 (de) * 1988-12-19 1990-06-21 Boehringer Mannheim Gmbh Testtraeger zur analytischen untersuchung einer probenfluessigkeit mit hilfe einer spezifischen bindungsreaktion zweier bioaffiner bindungspartner und entsprechendes testverfahren
US6352862B1 (en) 1989-02-17 2002-03-05 Unilever Patent Holdings B.V. Analytical test device for imuno assays and methods of using same
GB8903627D0 (en) * 1989-02-17 1989-04-05 Unilever Plc Assays
EP0588958A1 (en) * 1991-06-13 1994-03-30 Pacific Biotech Inc. Assay for the detection of specific ligands
DE4121493A1 (de) * 1991-06-28 1993-01-07 Draegerwerk Ag Analysevorrichtung zur quantitativen, immunologischen bestimmung von schadstoffen
FI92882C (fi) * 1992-12-29 1995-01-10 Medix Biochemica Ab Oy Kertakäyttöinen testiliuska ja menetelmä sen valmistamiseksi
FR2725023B1 (fr) * 1994-09-28 1997-01-31 Gks Technologies Diagnostic direct d'haptenes
JP3010699U (ja) * 1994-10-29 1995-05-02 株式会社ニッポンジーン 直接採尿の可能なクロマト式検査装置
FR2917171A1 (fr) * 2007-06-07 2008-12-12 Biosynex Sarl Methode de detection d'une molecule d'interet dans un echantillon liquide.
CN102095860B (zh) * 2011-01-25 2013-12-18 厦门大学附属中山医院 梅毒特异性IgG抗体免疫印迹试剂盒及其制备方法
WO2021099550A1 (de) 2019-11-22 2021-05-27 Nano Scale Machining GmbH Fluidmaschine, insbesondere hydromaschine

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2644249A1 (de) * 1976-09-30 1978-04-06 Abbott Lab Reaktive matrices
US4320111A (en) * 1977-06-14 1982-03-16 American Home Products Corporation Immunologic compositions methods of preparation and use
US4189304A (en) * 1978-10-27 1980-02-19 Miles Laboratories, Inc. Device and method for detecting myoglobin
US4331650A (en) * 1980-07-18 1982-05-25 Science Research Center, Inc. Identification of reagins in the blood serum of allergen sensitized vertebrates
JPS5818167A (ja) * 1981-07-24 1983-02-02 Fuji Photo Film Co Ltd 分析フイルム及びこれを用いる分析方法
US4446232A (en) * 1981-10-13 1984-05-01 Liotta Lance A Enzyme immunoassay with two-zoned device having bound antigens
US4447546A (en) * 1982-08-23 1984-05-08 Myron J. Block Fluorescent immunoassay employing optical fiber in capillary tube
DE3484505D1 (de) * 1983-12-19 1991-05-29 Daiichi Pure Chemicals Co Ltd Immuntest.

Also Published As

Publication number Publication date
IT1200382B (it) 1989-01-18
FI873398A0 (fi) 1987-08-05
HUT56632A (en) 1991-09-30
ES557803A0 (es) 1988-04-16
AU5511886A (en) 1986-08-26
ES8802258A1 (es) 1988-04-16
RO100056B1 (ro) 1990-10-30
ES8900067A1 (es) 1988-11-16
MW5287A1 (en) 1988-11-09
ES557818A0 (es) 1988-04-16
FI873398L (fi) 1987-08-05
JPS62501989A (ja) 1987-08-06
DD245727A5 (de) 1987-05-13
ES8802260A1 (es) 1988-04-16
EP0243370A1 (en) 1987-11-04
WO1986004683A1 (en) 1986-08-14
FI85194C (fi) 1992-03-10
RO100056A2 (ro) 1991-03-23
ES8802259A1 (es) 1988-04-16
FI85194B (fi) 1991-11-29
ES557662A0 (es) 1988-11-16
ES557802A0 (es) 1988-04-16
OA08640A (en) 1988-11-30
ES551760A0 (es) 1988-04-16
IT8519443A0 (it) 1985-02-08

Similar Documents

Publication Publication Date Title
ES8802260A1 (es) Un metodo y su correspondiente dispositivo para la determinacion de uno o mas anticuerpos simultaneamente en un liquido biologico
AU6762087A (en) Method of measuring antigens or antibodies in biological fluids using ligand labelled antigens or ligand labelled antibodies
AU559966B2 (en) Assaying antibody-antigen reactions
EP0279097A3 (en) Immunoassay test strip
AU559469B2 (en) Single incubation immunochemical assay
IL87882A (en) Method and kit for the immunometric determination of a surface antigen characteristic of cell population
EP0113075A3 (en) Field immunoassay reaction system, method of immunoassay diagnosis and probe or carrier for use therewith
KR880012768A (ko) 항-프리-s- 항체의 분석법
KR890006812A (ko) 메탐페타민에 대한 모노클로날 항체 그 모노클로날 항체의 제법 메탐페타민의 분석법 및 분석키트
EP0152254A3 (en) Chromogenic support immunoassay utilizing labeled complement components
GB8626767D0 (en) Assays
EP0158291A3 (en) A method for purifying carcinoembryonic antigen and a method for producing a carcinoembryonic antigen-reactive monoclonal antibody
EP0311456A3 (en) Monoclonal antibody to okadaic acids, process for producing the monoclonal antibody, assay reagent for assaying okadaic acids using the monoclonal antibody, and assay method
ES8701990A1 (es) Procedimiento para la determinacion de la capacidad de fijacion de la globulina fijadora de tiroxina
KR940000870A (ko) β-N- 아세틸글루코사미니다제의 샌드위치 면역 측정법 및 그 측정법에 사용된 모노클론 항체
NO884878D0 (no) Kvalitativ og kvantitativ bestemmelse av spesielle antigener, spesielt sporer av clostridium tyrobutyricum.
JPS54119026A (en) Simultaneous enzyme immunoassay and kit for practicing the same
AU5057785A (en) A method for determining a ligand
JPS6463864A (en) Immunoassay
JPS57131062A (en) Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier
JPS5386014A (en) Quantitative analysis of antigen and antibody
EP0389929A3 (en) Method for determining cell surface antigen in complexed antigen-antibody reaction system and immunocyte-fixation apparatus used in said method
JPS6463863A (en) Immunoassay
JPH04363662A (ja) 固定化担体の製造方法
EP0325782A3 (en) Method of measurement of cu,zn-superoxide dismutase

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20020401