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JPS6463864A - Immunoassay - Google Patents

Immunoassay

Info

Publication number
JPS6463864A
JPS6463864A JP22079787A JP22079787A JPS6463864A JP S6463864 A JPS6463864 A JP S6463864A JP 22079787 A JP22079787 A JP 22079787A JP 22079787 A JP22079787 A JP 22079787A JP S6463864 A JPS6463864 A JP S6463864A
Authority
JP
Japan
Prior art keywords
antibody
antigen
porous membrane
specimen
labelled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP22079787A
Other languages
Japanese (ja)
Inventor
Toshihiro Hiraoka
Masao Kitajima
Shigeki Kageyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Holdings Corp
Original Assignee
Fuji Photo Film Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Photo Film Co Ltd filed Critical Fuji Photo Film Co Ltd
Priority to JP22079787A priority Critical patent/JPS6463864A/en
Priority to EP88114356A priority patent/EP0308704A1/en
Publication of JPS6463864A publication Critical patent/JPS6463864A/en
Pending legal-status Critical Current

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To simplify operation, by containing a definite amount of an antigen or antibody labelled with enzyme in a porous membrane having an antibody or antigen insolubilized thereon and reacting a specimen therewith before measuring the enzymatic activity of a liquid phase by a dry chemical analysis film. CONSTITUTION:At first, a definite amount of an antibody or antigen is physically or chemically bonded to a porous membrane and the non-specific bonding region of the surface of the porous membrane is blocked by gelatin. A definite amount of an enzyme labelled antigen (or antibody) is contained in the porous membrane and a specimen is brought into contact with said antigen (or antibody) to be reacted therewith. Hereupon, the antigen (or antibody) in the specimen and the labelled antigen (or antibody) are competitively reacted with the insolubilized antibody (or antigen). Then, the amount of the non-bonding type enzyme labelled antigen (or antibody) present in the liquid phase after reaction is measured by a reaction speed method using a dry chemical analysis film containing all of reagents necessary for measurement of enzymatic activity.
JP22079787A 1987-09-03 1987-09-03 Immunoassay Pending JPS6463864A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP22079787A JPS6463864A (en) 1987-09-03 1987-09-03 Immunoassay
EP88114356A EP0308704A1 (en) 1987-09-03 1988-09-02 Heterogeneous enzymimmunoassay

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP22079787A JPS6463864A (en) 1987-09-03 1987-09-03 Immunoassay

Publications (1)

Publication Number Publication Date
JPS6463864A true JPS6463864A (en) 1989-03-09

Family

ID=16756716

Family Applications (1)

Application Number Title Priority Date Filing Date
JP22079787A Pending JPS6463864A (en) 1987-09-03 1987-09-03 Immunoassay

Country Status (1)

Country Link
JP (1) JPS6463864A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04500860A (en) * 1989-07-19 1992-02-13 ベーリング　ダイアグノスティクス　インコーポレイテッド Analytical assay method
US6129643A (en) * 1996-03-08 2000-10-10 Koyo Seiko Co., Ltd. Variable diameter pulley
JP2009145362A (en) * 2009-03-27 2009-07-02 Meiji Milk Prod Co Ltd Immunoassay for casein phosphopeptide (CPP)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04500860A (en) * 1989-07-19 1992-02-13 ベーリング　ダイアグノスティクス　インコーポレイテッド Analytical assay method
US6129643A (en) * 1996-03-08 2000-10-10 Koyo Seiko Co., Ltd. Variable diameter pulley
JP2009145362A (en) * 2009-03-27 2009-07-02 Meiji Milk Prod Co Ltd Immunoassay for casein phosphopeptide (CPP)

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