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ES2104835T3 - Metodo de tincion con un colorante acido. - Google Patents

Metodo de tincion con un colorante acido.

Info

Publication number
ES2104835T3
ES2104835T3 ES92305709T ES92305709T ES2104835T3 ES 2104835 T3 ES2104835 T3 ES 2104835T3 ES 92305709 T ES92305709 T ES 92305709T ES 92305709 T ES92305709 T ES 92305709T ES 2104835 T3 ES2104835 T3 ES 2104835T3
Authority
ES
Spain
Prior art keywords
acid dye
staining
acid
staining method
supporting matrix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92305709T
Other languages
English (en)
Inventor
Naoyuki Kouno
Jun Suzuoki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Application granted granted Critical
Publication of ES2104835T3 publication Critical patent/ES2104835T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Coloring (AREA)

Abstract

SE PRESENTA UN METODO PARA DECOLORAR UN TINTE ACIDO PARA SEPARAR ELECTROFORETICAMENTE FRACCIONES DE UNA MATRIZ DE SOPORTE QUE SE CARACTERIZA POR FIJAR LA MATRIZ DE SOPORTE DESPUES DE LA ELECTROFORESIS CON UNA SOLUCION ACUOSA QUE CONTENGA UN ALCOHOL INFERIOR Y UN ACIDO ORGANICO, SIGUIENDO CON LA DECOLORACION MEDIANTE UNA SOLUCION DE UN TINTE ACIDO MEZCLADA CON AL MENOS UN ACIDO ESPECIFICADO, EL METODO PUEDE REDUCIR EL TIEMPO REQUERIDO PARA DECOLORAR REMARCABLEMENTE Y MEJORAR LA SENSIBILIDAD.
ES92305709T 1991-06-24 1992-06-22 Metodo de tincion con un colorante acido. Expired - Lifetime ES2104835T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17878891 1991-06-24

Publications (1)

Publication Number Publication Date
ES2104835T3 true ES2104835T3 (es) 1997-10-16

Family

ID=16054654

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92305709T Expired - Lifetime ES2104835T3 (es) 1991-06-24 1992-06-22 Metodo de tincion con un colorante acido.

Country Status (8)

Country Link
US (1) US5284560A (es)
EP (1) EP0520725B1 (es)
JP (1) JP2803475B2 (es)
AT (1) ATE156909T1 (es)
CA (1) CA2071736A1 (es)
DE (1) DE69221550T2 (es)
DK (1) DK0520725T3 (es)
ES (1) ES2104835T3 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5567626A (en) * 1995-04-11 1996-10-22 Pall Corporation Method of detecting biological materials using a polyvinyllidene fluoride membrane
US6319720B1 (en) * 1998-10-13 2001-11-20 Ewald M. Wondrak Process for fast visualization of protein
DE10021390C2 (de) * 2000-05-03 2002-06-27 Juergen Olert Protektionslösung und Fixierverfahren für die Paraffinschnitt-Technik
JP4751605B2 (ja) * 2004-12-14 2011-08-17 株式会社アドバンス 電気泳動用染色液
CN101936837B (zh) * 2009-06-26 2012-09-05 南京金斯瑞生物科技有限公司 生物聚合物快速染色法
JP5750768B2 (ja) * 2012-01-25 2015-07-22 国立研究開発法人産業技術総合研究所 糖タンパク質又は多糖類の検出方法
CN104004382B (zh) * 2014-05-20 2016-01-20 北京五康新兴科技有限公司 一种考马斯亮蓝染色液和染色方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU561898A1 (ru) * 1974-12-09 1977-06-15 Всесоюзный Научно-Исследовательский Институт Мясной Промышленности Способ электрофоретического анализа белков
US4023933A (en) * 1976-06-10 1977-05-17 The University Of Georgia Protein-assay reagent and method
JPS6049256B2 (ja) * 1979-11-09 1985-10-31 大塚製薬株式会社 蛋白質分析用詩薬
JPS56103366A (en) * 1980-01-21 1981-08-18 Otsuka Pharmaceut Co Ltd Reagent for protein analysis and quantitative method of protein
SU951121A1 (ru) * 1980-10-29 1982-08-15 Институт химии им.В.И.Никитина Способ вы влени белков
US4405720A (en) * 1981-03-04 1983-09-20 The United States Of America As Represented By The Department Of Health And Human Services Silver stains for protein in gels
US5064768A (en) * 1988-09-16 1991-11-12 Daiichi Pure Chemicals Co., Ltd. Silver staining technique and kit

Also Published As

Publication number Publication date
JP2803475B2 (ja) 1998-09-24
JPH05281190A (ja) 1993-10-29
US5284560A (en) 1994-02-08
CA2071736A1 (en) 1992-12-25
EP0520725A2 (en) 1992-12-30
ATE156909T1 (de) 1997-08-15
EP0520725A3 (es) 1994-03-09
EP0520725B1 (en) 1997-08-13
DE69221550D1 (de) 1997-09-18
DE69221550T2 (de) 1998-02-19
DK0520725T3 (da) 1997-09-22

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