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ES2051651B1 - Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones. - Google Patents

Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones.

Info

Publication number
ES2051651B1
ES2051651B1 ES09300001A ES9300001A ES2051651B1 ES 2051651 B1 ES2051651 B1 ES 2051651B1 ES 09300001 A ES09300001 A ES 09300001A ES 9300001 A ES9300001 A ES 9300001A ES 2051651 B1 ES2051651 B1 ES 2051651B1
Authority
ES
Spain
Prior art keywords
procedure
human lymphocytes
single measurement
populations
sub
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES09300001A
Other languages
English (en)
Other versions
ES2051651A1 (es
Inventor
De Matos Correira E Vale Orfao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Salamanca
Original Assignee
Universidad de Salamanca
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES09300001A priority Critical patent/ES2051651B1/es
Application filed by Universidad de Salamanca filed Critical Universidad de Salamanca
Priority to DE69328412T priority patent/DE69328412T2/de
Priority to AT93500135T priority patent/ATE191974T1/de
Priority to DK93500135T priority patent/DK0603107T3/da
Priority to PT93500135T priority patent/PT603107E/pt
Priority to EP93500135A priority patent/EP0603107B1/en
Priority to US08/163,540 priority patent/US5538855A/en
Publication of ES2051651A1 publication Critical patent/ES2051651A1/es
Application granted granted Critical
Publication of ES2051651B1 publication Critical patent/ES2051651B1/es
Priority to GR20000401496T priority patent/GR3033797T3/el
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

PROCEDIMIENTO PARA LA CUANTIFICACION SIMULTANEA, EN UNA SOLA MEDICION, DE LOS PRINCIPALES TIPOS DE LINFOCITOS HUMANOS Y SUS SUBPOBLACIONES. COMPRENDE: INCUBAR LA MUESTRA DE SANGRE CON CINCO ANTICUERPOS MONOCLONALES DIFERENTES MARCADOS CON TRES SUSTANCIAS FLUORESCENTES DISTINTAS; MEDIR POR CITOMETRIA DE FLUJO LAS TRES EMISIONES FLUORESCENTES DIFERENTES; Y ANALIZAR LOS RESULTADOS OBTENIDOS MEDIANTE ANALISIS MULTIPARAMETRICO PARA DETERMINAR LA PRESENCIA/AUSENCIA EN LAS CELULAS DE UNA SECUENCIA FLUORESCENTE CONCRETA Y LA INTENSIDAD DE ESTA EN CADA CELULA.
ES09300001A 1992-12-10 1992-12-10 Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones. Expired - Fee Related ES2051651B1 (es)

Priority Applications (8)

Application Number Priority Date Filing Date Title
ES09300001A ES2051651B1 (es) 1992-12-10 1992-12-10 Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones.
AT93500135T ATE191974T1 (de) 1992-12-10 1993-10-04 Verfahren zur simultanen, quantitativen bestimmung, in einer einzelnen messung, der haupttypen der menschlichen lymphozyten und deren untereinheiten
DK93500135T DK0603107T3 (da) 1992-12-10 1993-10-04 Fremgangsmåde til samtidig kvantificering af de vigtigste typer af humane lymfocytter og deres deltyper
PT93500135T PT603107E (pt) 1992-12-10 1993-10-04 Processo para a quantificacao simultanea numa unica medicao dos principais tipos de linfocitos humanos e dos seus subconjuntos
DE69328412T DE69328412T2 (de) 1992-12-10 1993-10-04 Verfahren zur simultanen, quantitativen Bestimmung, in einer einzelnen Messung, der Haupttypen der menschlichen Lymphozyten und deren Untereinheiten
EP93500135A EP0603107B1 (en) 1992-12-10 1993-10-04 Procedure for the simultaneous quantification, in a single measurement, of the major types of human lymphocytes and their subsets
US08/163,540 US5538855A (en) 1992-12-10 1993-12-09 Procedure for the simultaneous quantification, in a single measurement, of the major types of human lymphocytes and their subsets
GR20000401496T GR3033797T3 (en) 1992-12-10 2000-06-28 Procedure for the simultaneous quantification, in a single measurement, of the major types of human lymphocytes and their subsets.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09300001A ES2051651B1 (es) 1992-12-10 1992-12-10 Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones.

Publications (2)

Publication Number Publication Date
ES2051651A1 ES2051651A1 (es) 1994-06-16
ES2051651B1 true ES2051651B1 (es) 1995-01-01

Family

ID=8280378

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09300001A Expired - Fee Related ES2051651B1 (es) 1992-12-10 1992-12-10 Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones.

Country Status (8)

Country Link
US (1) US5538855A (es)
EP (1) EP0603107B1 (es)
AT (1) ATE191974T1 (es)
DE (1) DE69328412T2 (es)
DK (1) DK0603107T3 (es)
ES (1) ES2051651B1 (es)
GR (1) GR3033797T3 (es)
PT (1) PT603107E (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2112211A1 (es) * 1996-03-26 1998-03-16 Univ Salamanca Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas.

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2125228A1 (en) * 1993-06-08 1994-12-09 Thomas J. Mercolino Three-color flow cytometry with automatic gating function
US5994089A (en) 1997-05-16 1999-11-30 Coulter International Corp. Simultaneous analyses of white blood cell subsets using multi-color, multi-intensity fluorescent markers in flow cytometry
US6251615B1 (en) 1998-02-20 2001-06-26 Cell Analytics, Inc. Cell analysis methods
BR9916449A (pt) * 1998-12-23 2002-01-22 Univ Sydney Dispositivo de ensaio, métodos para determinação de uma condição de doença ou de um distúrbio ou de uma propensão para o desenvolvimento de uma condição de doença ou de um distúrbio e para tratamento de câncer ou de uma propensão para o desenvolvimento de câncer em um animal humano ou não-humano, uso de um arranjo de moléculas, e, método para identificação de uma proteìna mimética em uma amostra quìmica ou biológica
DE19935047C2 (de) * 1999-07-26 2001-05-31 Johannes L J Frank Multidimensionale Dynamische Cytofluorophorese (MDCFP)
US6506551B1 (en) 1999-10-08 2003-01-14 North Shore - Long Island Jewish Research Institute CD38 as a prognostic indicator in B cell chronic lymphocytic leukemia
US8323903B2 (en) * 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
US20050069962A1 (en) 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
ES2198203B1 (es) * 2002-02-25 2005-04-01 Universidad De Cordoba Nuevos metodos analiticos basados en la expresion de determinados receptores de celulas nk en celulas t cd8.
US7321843B2 (en) * 2005-09-30 2008-01-22 Universidad De Salamanca Method for generating new flow cytometry data files containing an infinite number of dimensions based on data estimation
EP2259065A1 (en) * 2009-06-03 2010-12-08 Erasmus University Medical Center Rotterdam Methods, reagents and kits for flow cytometric immunophenotyping
CN107422122B (zh) 2012-06-14 2020-06-26 鹿特丹伊拉斯姆斯大学医疗中心 用于检测微小残留病的方法、试剂以及试剂盒
CN110716051B (zh) * 2018-07-13 2022-11-29 迈健医药科技无锡有限公司 一种免疫细胞全维分析方法
US20220082489A1 (en) 2020-06-26 2022-03-17 Cytek Biosciences, Inc. Methods and apparatus for full spectrum flow cytometer

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4499052A (en) * 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
DE3322373C2 (de) * 1983-05-19 1986-12-04 Ioannis Dr. 3000 Hannover Tripatzis Testmittel und Verfahren zum Nachweis von Antigenen und/oder Antikörpern
US4599307A (en) * 1983-07-18 1986-07-08 Becton, Dickinson And Company Method for elimination of selected cell populations in analytic cytology
EP0182874B1 (en) * 1984-05-31 1993-10-20 Coulter Corporation A reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes
CA1279008C (en) * 1985-10-11 1991-01-15 Smith Kline & French Canada Ltd. Methods and reagents for performing subset analysis
US4745285A (en) * 1986-08-21 1988-05-17 Becton Dickinson And Company Multi-color fluorescence analysis with single wavelength excitation
ES2035317T5 (es) * 1987-11-09 1998-03-16 Becton Dickinson Co Metodo para analizar celulas hematopoyeticas en una muestra.
US4987086A (en) * 1987-11-30 1991-01-22 Becton, Dickinson And Company Method for analysis of subpopulations of cells
US4895796A (en) * 1988-01-06 1990-01-23 Becton Dickinson And Company Identification of NK cells and cytotoxic T lymphocytes
IE76732B1 (en) * 1990-08-07 1997-11-05 Becton Dickinson Co One step test for absolute counts
GR920100123A (el) * 1991-04-12 1993-03-16 Ortho Diagnostic Systems Inc Αντιδραστήριο πολυχρωματικής κηλίδωσης και μέ?οδος ανάλυσης.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2112211A1 (es) * 1996-03-26 1998-03-16 Univ Salamanca Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas.

Also Published As

Publication number Publication date
DE69328412D1 (de) 2000-05-25
ES2051651A1 (es) 1994-06-16
PT603107E (pt) 2000-10-31
EP0603107B1 (en) 2000-04-19
EP0603107A2 (en) 1994-06-22
DE69328412T2 (de) 2000-11-09
ATE191974T1 (de) 2000-05-15
US5538855A (en) 1996-07-23
DK0603107T3 (da) 2000-09-18
EP0603107A3 (en) 1995-05-10
GR3033797T3 (en) 2000-10-31

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Effective date: 20141120