ES2193095T3 - Procedimiento de cuantificacion de microparticulas endoteliales. - Google Patents
Procedimiento de cuantificacion de microparticulas endoteliales.Info
- Publication number
- ES2193095T3 ES2193095T3 ES00948067T ES00948067T ES2193095T3 ES 2193095 T3 ES2193095 T3 ES 2193095T3 ES 00948067 T ES00948067 T ES 00948067T ES 00948067 T ES00948067 T ES 00948067T ES 2193095 T3 ES2193095 T3 ES 2193095T3
- Authority
- ES
- Spain
- Prior art keywords
- fluorochrome
- microspheres
- population
- microparticles
- marked
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000011859 microparticle Substances 0.000 title abstract 5
- 230000003511 endothelial effect Effects 0.000 title abstract 2
- 238000011002 quantification Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 abstract 6
- 239000004005 microsphere Substances 0.000 abstract 5
- 239000000203 mixture Substances 0.000 abstract 3
- 238000004458 analytical method Methods 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 1
- 230000004069 differentiation Effects 0.000 abstract 1
- 238000009792 diffusion process Methods 0.000 abstract 1
- 210000002889 endothelial cell Anatomy 0.000 abstract 1
- 238000000684 flow cytometry Methods 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1012—Calibrating particle analysers; References therefor
- G01N2015/1014—Constitution of reference particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1024—Counting particles by non-optical means
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Peptides Or Proteins (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Monorreactivo para la detección y la cuantificación de las micropartículas de origen endotelial en una muestra plasmática por citometría de flujo, que comprende (i) una mezcla de anticuerpos monoclonales específicos de las moléculas de membrana de las células endoteliales, dicha mezcla que comprende un anticuerpo (1) marcado con un fluorocromo 1 y un anticuerpo (2) marcado con un fluorocromo 2, dichos anticuerpos (1) y (2) no presentan la misma especificidad y ii) una mezcla de microesferas que comprende: - una población A de microesferas (patrón) marcada con un fluorocromo que sirve para definir la región de análisis de las micropartículas en término de tamaño (parámetros de difusión luminosa) y que permite su diferenciación, - una población de microesferas B (patrón) cuyo número es conocido que sirve para el recuento de las micropartículas, - una población de microesferas C marcada únicamente con el fluorocromo 1, que sirve para definir sobre el parámetro de fluorescencia del fluorocromo1 el umbral de intensidad mínima que caracteriza las micropartículas marcadas con los anticuerpos- fluorocromo 1, - y una población de microesferas D, de diámetro idéntico a C, y marcada únicamente con el fluorocromo 2, que sirve para definir la región de análisis de los acontecimientos considerados positivos para el
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9908505A FR2795820A1 (fr) | 1999-07-01 | 1999-07-01 | Procede de quantification de microparticules endotheliales |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2193095T3 true ES2193095T3 (es) | 2003-11-01 |
Family
ID=9547610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00948067T Expired - Lifetime ES2193095T3 (es) | 1999-07-01 | 2000-06-30 | Procedimiento de cuantificacion de microparticulas endoteliales. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1192443B1 (es) |
| AT (1) | ATE239221T1 (es) |
| DE (1) | DE60002466T2 (es) |
| DK (1) | DK1192443T3 (es) |
| ES (1) | ES2193095T3 (es) |
| FR (1) | FR2795820A1 (es) |
| WO (1) | WO2001002835A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2809181B1 (fr) * | 2000-05-16 | 2002-10-25 | Biocytex | Monoreactif pour le dosage des microparticules plaquettaires |
| ES2178594B1 (es) * | 2001-02-20 | 2004-09-16 | Universidad De Salamanca | Procedimiento para controlar el recuento del numero absoluto de celulas u otras particulas presentes en una muestra. |
| FR2917172B1 (fr) * | 2007-06-07 | 2014-01-03 | Inst Nat Sante Rech Med | Methode de mesure de l'activite plasmine des microparticules presentes dans un echantillon de fluide biologique et utilisation |
| US10809269B2 (en) | 2015-04-21 | 2020-10-20 | Centre Hospitalier Universitaire De Nimes | Use of soluble CD146 as a biomarker to select in vitro-fertilized embryo for implantation in a mammal |
| CN106092860A (zh) * | 2016-05-29 | 2016-11-09 | 江苏英诺华医疗技术有限公司 | 一种血小板活化率的检测方法 |
| CN108982872B (zh) * | 2018-07-25 | 2022-04-05 | 苏州丰泰医疗用品贸易有限公司 | 一种基于微球和荧光标记的快速检测抗原或抗体方法 |
-
1999
- 1999-07-01 FR FR9908505A patent/FR2795820A1/fr not_active Withdrawn
-
2000
- 2000-06-30 ES ES00948067T patent/ES2193095T3/es not_active Expired - Lifetime
- 2000-06-30 DE DE60002466T patent/DE60002466T2/de not_active Expired - Lifetime
- 2000-06-30 WO PCT/FR2000/001868 patent/WO2001002835A1/fr active IP Right Grant
- 2000-06-30 AT AT00948067T patent/ATE239221T1/de not_active IP Right Cessation
- 2000-06-30 DK DK00948067T patent/DK1192443T3/da active
- 2000-06-30 EP EP00948067A patent/EP1192443B1/fr not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| FR2795820A1 (fr) | 2001-01-05 |
| ATE239221T1 (de) | 2003-05-15 |
| EP1192443A1 (fr) | 2002-04-03 |
| DE60002466T2 (de) | 2004-04-01 |
| DE60002466D1 (de) | 2003-06-05 |
| EP1192443B1 (fr) | 2003-05-02 |
| DK1192443T3 (da) | 2003-08-04 |
| WO2001002835A1 (fr) | 2001-01-11 |
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