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EP3286296A4 - Mikrofluidische zellkultur - Google Patents

Mikrofluidische zellkultur Download PDF

Info

Publication number
EP3286296A4
EP3286296A4 EP16783924.0A EP16783924A EP3286296A4 EP 3286296 A4 EP3286296 A4 EP 3286296A4 EP 16783924 A EP16783924 A EP 16783924A EP 3286296 A4 EP3286296 A4 EP 3286296A4
Authority
EP
European Patent Office
Prior art keywords
cell culture
microfluidic cell
microfluidic
culture
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP16783924.0A
Other languages
English (en)
French (fr)
Other versions
EP3286296A1 (de
Inventor
Randall D. LOWE, Jr.
Kristin BEAUMONT
Aathavan KARUNAKARAN
Natalie MARKS
Jason M. MCEWEN
Mark P. White
J. Tanner Nevill
Gang F. WANG
Andrew W. Mcfarland
Daniele Malleo
Keith J. Breinlinger
Xiao GUAN
Kevin T. Chapman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bruker Cellular Analysis Inc
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Priority claimed from PCT/US2016/028808 external-priority patent/WO2016172454A1/en
Publication of EP3286296A1 publication Critical patent/EP3286296A1/de
Publication of EP3286296A4 publication Critical patent/EP3286296A4/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP16783924.0A 2015-04-22 2016-04-22 Mikrofluidische zellkultur Pending EP3286296A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562151325P 2015-04-22 2015-04-22
PCT/US2016/028808 WO2016172454A1 (en) 2015-04-22 2016-04-22 Microfluidic cell structure

Publications (2)

Publication Number Publication Date
EP3286296A1 EP3286296A1 (de) 2018-02-28
EP3286296A4 true EP3286296A4 (de) 2019-01-09

Family

ID=58055846

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16783924.0A Pending EP3286296A4 (de) 2015-04-22 2016-04-22 Mikrofluidische zellkultur

Country Status (4)

Country Link
EP (1) EP3286296A4 (de)
CN (2) CN114085730A (de)
HK (1) HK1253068A1 (de)
TW (2) TWI819383B (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102686863B1 (ko) * 2017-01-31 2024-07-19 알피니티 유에스에이, 인크. 일체형 펌프를 갖는 바이오프로세스 용기
CN108485972B (zh) * 2018-03-28 2021-06-25 东南大学 一种用于细胞组织培养与实时监测的微流控芯片及其使用方法
CN112703059A (zh) * 2018-07-12 2021-04-23 伯克利之光生命科技公司 针对抗病性状筛选植物原生质体
CN116174068A (zh) 2019-04-30 2023-05-30 伯克利之光生命科技公司 用于包封和测定细胞的方法
AU2020297612A1 (en) * 2019-06-20 2022-02-03 President And Fellows Of Harvard College Isolating live cells after high-throughput, long-term, time-lapse microscopy
JP7391339B2 (ja) * 2019-08-29 2023-12-05 ファナック株式会社 細胞製造装置及びその製造方法
EP4048626A4 (de) * 2019-10-25 2023-12-06 Berkeley Lights, Inc. Systeme zum betrieb von mikrofluidischen vorrichtungen
JP7339138B2 (ja) * 2019-12-02 2023-09-05 株式会社日立製作所 細胞培養装置
US20220348863A1 (en) * 2021-04-28 2022-11-03 A.U. Vista, Inc. Tft-based cell isolation device and cell manipulation panel thereof
CN113996355B (zh) * 2021-10-28 2023-06-06 上海浚真生命科学有限公司 取样装置
US12252678B2 (en) 2021-12-01 2025-03-18 Microfluidx Ltd Systems and methods for bioprocessing
TWI832371B (zh) * 2022-08-10 2024-02-11 醫華生技股份有限公司 生物微粒培養設備及其微結構
TWI859685B (zh) * 2022-12-30 2024-10-21 財團法人工業技術研究院 微流體結構、可攜式核酸檢測套組以及於戶外檢測樣本的核酸的方法
TWI849811B (zh) * 2023-03-21 2024-07-21 好孕行生醫股份有限公司 用以提供垂直溫差環境之加熱培養裝置及其使用方法
TWI848840B (zh) * 2023-10-25 2024-07-11 財團法人金屬工業研究發展中心 細胞改質系統及裝置
CN117262436B (zh) * 2023-11-21 2024-01-26 山东大学齐鲁医院 一种肿瘤切片存储装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060226012A1 (en) * 2005-04-08 2006-10-12 Kanagasabapathi Thirukumaran T Integrated microfluidic transport and sorting system
WO2015188171A1 (en) * 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
WO2016090295A1 (en) * 2014-12-05 2016-06-09 The Regents Of The University Of California Single-sided light-actuated microfluidic device with integrated mesh ground

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW550066B (en) * 2000-12-01 2003-09-01 Ind Tech Res Inst Micro bio-cultivation device
US6982165B2 (en) * 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
US6972173B2 (en) * 2002-03-14 2005-12-06 Intel Corporation Methods to increase nucleotide signals by raman scattering
US6984485B2 (en) * 2002-04-23 2006-01-10 Beckman Coulter, Inc. Polymer-coated substrates for immobilization of biomolecules and cells
US7790443B2 (en) * 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
US20080257735A1 (en) * 2004-09-24 2008-10-23 Regents Of The University Of California Microfluidic Device for Enabling the Controlled Growth of Cells and Methods Relating to Same
US20060252087A1 (en) * 2005-01-18 2006-11-09 Biocept, Inc. Recovery of rare cells using a microchannel apparatus with patterned posts
US8475886B2 (en) * 2005-08-05 2013-07-02 Corning Incorporated Methods for producing surfaces that resist non-specific protein binding and cell attachment
EP1997877B1 (de) * 2006-03-17 2014-03-05 Sanyo Chemical Industries, Ltd. Zellkultursubstrat
KR100903969B1 (ko) * 2007-06-12 2009-06-25 삼성전자주식회사 마이크로 어레이, 마이크로 어레이용 기판 및 이들의 제조방법
CN101275114A (zh) * 2008-04-22 2008-10-01 北京大学 一种微流细胞培养阵列及其应用
TW201144805A (en) * 2010-06-08 2011-12-16 Academia Sinica Microfluidic device
CN102296029B (zh) * 2010-06-28 2013-01-30 裴国献 灌注式生物反应器系统
US20140154703A1 (en) * 2011-01-06 2014-06-05 Alison Skelley Circulating tumor cell capture on a microfluidic chip incorporating both affinity and size
US9656874B2 (en) * 2011-01-11 2017-05-23 Nippon Sheet Glass Company, Limited Method for producing metal oxide-containing particles, and method for producing aggregates of metal oxide colloidal particles
WO2012109068A2 (en) * 2011-02-11 2012-08-16 Corning Incorporated Enzyme cleavable cell release polymeric surface
CN103502426B (zh) * 2011-02-28 2018-03-13 哈佛大学校长及研究员协会 细胞培养系统
TWI438273B (zh) * 2011-03-08 2014-05-21 Univ Chang Gung High-throughput perfusative microfluidic cell culture wafers for miniaturized three-dimensional cell culture
TW201310016A (zh) * 2011-08-26 2013-03-01 Chin-Feng Wan 生物檢測晶片及其製造方法
WO2013148745A1 (en) * 2012-03-28 2013-10-03 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
US9857333B2 (en) * 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
CN204079986U (zh) * 2014-05-30 2015-01-07 南京农业大学 一种用于细胞迁移研究的微纳流控器件

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060226012A1 (en) * 2005-04-08 2006-10-12 Kanagasabapathi Thirukumaran T Integrated microfluidic transport and sorting system
US7658829B2 (en) * 2005-04-08 2010-02-09 Uti Limited Partnership Integrated microfluidic transport and sorting system
WO2015188171A1 (en) * 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
WO2016090295A1 (en) * 2014-12-05 2016-06-09 The Regents Of The University Of California Single-sided light-actuated microfluidic device with integrated mesh ground

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2016172454A1 *

Also Published As

Publication number Publication date
CN107849505A (zh) 2018-03-27
CN114085730A (zh) 2022-02-25
EP3286296A1 (de) 2018-02-28
CN107849505B (zh) 2021-09-07
TWI744230B (zh) 2021-11-01
TW202233824A (zh) 2022-09-01
HK1253068A1 (zh) 2019-06-06
TW201643241A (zh) 2016-12-16
TWI819383B (zh) 2023-10-21

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