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EA201891697A1 - Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк - Google Patents

Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк

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Publication number
EA201891697A1
EA201891697A1 EA201891697A EA201891697A EA201891697A1 EA 201891697 A1 EA201891697 A1 EA 201891697A1 EA 201891697 A EA201891697 A EA 201891697A EA 201891697 A EA201891697 A EA 201891697A EA 201891697 A1 EA201891697 A1 EA 201891697A1
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Eurasian Patent Office
Prior art keywords
nucleic acid
rna
dna
decoding
terminal region
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EA201891697A
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English (en)
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EA037304B1 (ru
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Ясухиро Муракава
Юдзиро Такегами
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КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ
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Publication of EA201891697A1 publication Critical patent/EA201891697A1/ru
Publication of EA037304B1 publication Critical patent/EA037304B1/ru

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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Изобретение предлагает способ анализа, который позволяет идентифицировать элемент ДНК с повышенной чувствительностью и определить уровень транскрипции элемента ДНК. Настоящее изобретение относится к способу декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку РНК, включающему стадию получения-декодирования способа получения с использованием матричной РНК по меньшей мере одной нуклеиновой кислоты, выбранной из группы, состоящей из нуклеиновой кислоты РНК 5'-концевого участка матричной РНК или комплементарной нуклеиновой кислоты ДНК, соответствующей 5'-концевому участку матричной РНК; нуклеиновой кислоты РНК 3'-концевого участка матричной РНК или комплементарной нуклеиновой кислоты ДНК, соответствующей 3'-концевому участку матричной РНК; нуклеиновой кислоты РНК частичного участка антисмысловой цепи или смысловой цепи полноразмерной матричной РНК; и их сочетаний, или нуклеиновой кислоты, последовательность которой содержит две или более связанных последовательностей, где матричная РНК, используемая на стадии получения-декодирования, представляет собой растущую цепь РНК. Элемент ДНК можно идентифицировать и проанализировать с повышенной точностью и повышенной чувствительностью путем определения информации о декодированной последовательности нуклеиновой кислоты и картирования элемента ДНК в геноме.
EA201891697A 2016-01-27 2017-01-13 Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк EA037304B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016013575 2016-01-27
PCT/JP2017/001097 WO2017130750A1 (ja) 2016-01-27 2017-01-13 Ｒｎａの末端領域に対応する核酸の塩基配列を解読する方法およびｄｎａエレメントの分析方法

Publications (2)

Publication Number Publication Date
EA201891697A1 true EA201891697A1 (ru) 2019-01-31
EA037304B1 EA037304B1 (ru) 2021-03-09

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EA201891697A EA037304B1 (ru) 2016-01-27 2017-01-13 Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк

Country Status (6)

Country Link
US (1) US11312991B2 (ru)
EP (1) EP3409773B1 (ru)
JP (1) JP6417603B2 (ru)
DK (1) DK3409773T3 (ru)
EA (1) EA037304B1 (ru)
WO (1) WO2017130750A1 (ru)

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US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6280935B1 (en) 1994-10-13 2001-08-28 Lynx Therapeutics, Inc. Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
FR2733762B1 (fr) 1995-05-02 1997-08-01 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6013488A (en) 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
EP1197552B1 (en) 2000-08-25 2006-11-08 Riken Method of preparing normalized and/or subtracted cDNA
AU2003238702A1 (en) 2002-06-12 2003-12-31 Kabushiki Kaisha Dnaform Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
JP4403069B2 (ja) 2002-08-12 2010-01-20 独立行政法人理化学研究所 クローニングおよび分析のためのｍＲＮＡの５’末端の使用方法
JP2009072062A (ja) 2006-04-07 2009-04-09 Institute Of Physical & Chemical Research 核酸の５’末端を単離するための方法およびその適用
US20150184246A1 (en) * 2011-11-11 2015-07-02 Millennium Pharmaceuticals, Inc. Biomarkers of response to proteasome inhibitors
US9333578B2 (en) 2014-06-30 2016-05-10 General Electric Company Fiber reinforced brazed components and methods

Also Published As

Publication number Publication date
JP6417603B2 (ja) 2018-11-07
DK3409773T3 (da) 2020-09-07
EP3409773B1 (en) 2020-07-29
US20190032127A1 (en) 2019-01-31
WO2017130750A1 (ja) 2017-08-03
EP3409773A4 (en) 2019-07-03
EA037304B1 (ru) 2021-03-09
EP3409773A1 (en) 2018-12-05
US11312991B2 (en) 2022-04-26
JPWO2017130750A1 (ja) 2018-02-01

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