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ATE545712T1 - Verfahren mit verwendung von poren - Google Patents

Verfahren mit verwendung von poren

Info

Publication number
ATE545712T1
ATE545712T1 AT06808555T AT06808555T ATE545712T1 AT E545712 T1 ATE545712 T1 AT E545712T1 AT 06808555 T AT06808555 T AT 06808555T AT 06808555 T AT06808555 T AT 06808555T AT E545712 T1 ATE545712 T1 AT E545712T1
Authority
AT
Austria
Prior art keywords
nucleotide
pore
relates
pores
identity
Prior art date
Application number
AT06808555T
Other languages
English (en)
Inventor
Hagan Bayley
Yann Astier
Orit Braha
Original Assignee
Isis Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation filed Critical Isis Innovation
Application granted granted Critical
Publication of ATE545712T1 publication Critical patent/ATE545712T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT06808555T 2005-11-15 2006-11-15 Verfahren mit verwendung von poren ATE545712T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0523282.2A GB0523282D0 (en) 2005-11-15 2005-11-15 Methods using pores
PCT/GB2006/004265 WO2007057668A1 (en) 2005-11-15 2006-11-15 Methods using pores

Publications (1)

Publication Number Publication Date
ATE545712T1 true ATE545712T1 (de) 2012-03-15

Family

ID=35516986

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06808555T ATE545712T1 (de) 2005-11-15 2006-11-15 Verfahren mit verwendung von poren

Country Status (16)

Country Link
US (2) US8105846B2 (de)
EP (2) EP1951898B1 (de)
JP (1) JP5328362B2 (de)
KR (1) KR20080083281A (de)
CN (1) CN101356288B (de)
AT (1) ATE545712T1 (de)
AU (1) AU2006314296B2 (de)
BR (1) BRPI0618391A2 (de)
CA (1) CA2629009C (de)
GB (1) GB0523282D0 (de)
IL (1) IL191319A (de)
MY (1) MY149161A (de)
NZ (1) NZ568163A (de)
RU (1) RU2008123842A (de)
UA (1) UA95087C2 (de)
WO (1) WO2007057668A1 (de)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP2158476B8 (de) 2007-05-08 2019-10-09 Trustees of Boston University Chemische funktionalisierung von festkörper-nanoporen und nanoporen-arrays sowie anwendungen davon
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
US20110229877A1 (en) * 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2010004273A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
EP2851433B1 (de) * 2008-08-01 2017-10-11 Brown University System und Verfahren zur Bestimmung von Molekülen anhand von Massenspektrometrie sowie entsprechende Verfahren
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
WO2010062903A2 (en) * 2008-11-26 2010-06-03 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
EP2391732B1 (de) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Verfahren die adaptoren zur nukleinsaure constructen für transmembrane sequenzierung benutzen
CN102482330A (zh) * 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US9017937B1 (en) 2009-04-10 2015-04-28 Pacific Biosciences Of California, Inc. Nanopore sequencing using ratiometric impedance
US8986928B2 (en) * 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CA2808576A1 (en) 2009-09-30 2011-04-07 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
AU2010326349B2 (en) 2009-12-01 2015-10-29 Oxford Nanopore Technologies Limited Biochemical analysis instrument
US9005425B2 (en) 2010-03-05 2015-04-14 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
WO2011109825A2 (en) * 2010-03-05 2011-09-09 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
US8673757B2 (en) 2010-10-28 2014-03-18 International Business Machines Corporation Structure and method for using high-k material as an etch stop layer in dual stress layer process
BR112013020411B1 (pt) 2011-02-11 2021-09-08 Oxford Nanopore Technologies Limited Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo
US10246741B2 (en) 2011-05-27 2019-04-02 Oxford Nanopore Technologies Ltd. Coupling method
RU2467218C1 (ru) * 2011-06-16 2012-11-20 Закрытое Акционерное Общество "Научно-производственное объединение "Гидравлические аппараты" (ЗАО "НПО "Гидроаппарат") Гидромуфта
CN104774757B (zh) * 2011-07-20 2017-03-01 加利福尼亚大学董事会 双孔装置
KR20140050067A (ko) 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
EP2987870B1 (de) * 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Verfahren zur kennzeichnung eines zielpolynukleotids mit einer transmembranpore und eines molekularen motors
EP2798083B1 (de) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Verfahren zur charakterisierung einer polynukelotids mittels verwendung einer xpd-helikase
JP6228128B2 (ja) 2011-12-29 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
US10739341B2 (en) 2012-02-15 2020-08-11 Oxford Nanopore Technologies Limited Aptamer method
SG11201405995YA (en) * 2012-04-02 2014-10-30 Lux Bio Group Inc Apparatus and method for molecular separation, purification, and sensing
US9777049B2 (en) 2012-04-10 2017-10-03 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
WO2014013262A1 (en) * 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
US9995728B2 (en) 2012-11-06 2018-06-12 Oxford Nanopore Technologies Ltd. Quadruplex method
EP2964779B1 (de) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Verwendung von spacer-elementen in einer nukleinsäure zur kontrolle der bewegung einer helicase
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN103267785B (zh) * 2013-05-14 2016-04-13 西北大学 一种检测药物包裹或释放过程的单分子分析方法
PT3004378T (pt) 2013-05-24 2018-03-06 Illumina Cambridge Ltd Resumo
US9862997B2 (en) 2013-05-24 2018-01-09 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed FRET detection
GB201316849D0 (en) 2013-09-23 2013-11-06 Isis Innovation Method
CN117947148A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
JP6749243B2 (ja) 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3137627A1 (de) 2014-05-02 2017-03-08 Oxford Nanopore Technologies Limited Verfahren zur verbesserung der bewegung eines zielpolynukleotids bezüglich einer transmembranpore
GB201411285D0 (en) 2014-06-25 2014-08-06 Prosser Joseph Sequencer
JP7161291B2 (ja) 2014-09-01 2022-10-26 ブイアイビー ブイゼットダブリュ 変異体ポア
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
CN107109472B (zh) 2014-10-10 2021-05-11 昆塔波尔公司 利用互相猝灭的荧光标记物的基于纳米孔的聚合物分析
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
EP3209800B1 (de) 2014-10-24 2022-02-16 Quantapore, Inc. Effiziente optische analyse von polymeren unter verwendung von nanostrukturanordnungen
WO2016069806A2 (en) 2014-10-31 2016-05-06 Genia Technologies, Inc. Alpha-hemolysin variants with altered characteristics
US10060903B2 (en) * 2014-11-05 2018-08-28 Genia Technologies, Inc. Exporting measurements of nanopore arrays
US9863904B2 (en) 2014-12-19 2018-01-09 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
US9557294B2 (en) 2014-12-19 2017-01-31 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
CN107209182A (zh) * 2015-02-02 2017-09-26 双孔人公司 用于生物标志物检测的不稳定的接头
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
EP3283887B1 (de) * 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanoporen mit internen proteinadaptoren
WO2017050718A1 (en) * 2015-09-24 2017-03-30 Genia Technologies, Inc. Alpha-hemolysin variants
US10126262B2 (en) 2015-09-24 2018-11-13 Genia Technologies, Inc. Differential output of analog memories storing nanopore measurement samples
CN105259229B (zh) * 2015-10-22 2018-04-20 清华大学 一种检测药物的单分子分析方法
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔，包含其的组合物及其应用
JP6579948B2 (ja) * 2015-12-24 2019-09-25 株式会社日立ハイテクノロジーズ 生体ポリマを分析するための測定試薬及び分析デバイス
CN116356000A (zh) 2016-03-02 2023-06-30 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
CN118326019A (zh) 2016-04-06 2024-07-12 牛津纳米孔科技公开有限公司 突变体孔
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP3464616B1 (de) 2016-05-25 2022-05-04 Oxford Nanopore Technologies plc Verfahren
US10823721B2 (en) 2016-07-05 2020-11-03 Quantapore, Inc. Optically based nanopore sequencing
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
AU2018218531B2 (en) * 2017-02-10 2021-01-28 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
WO2018195222A1 (en) * 2017-04-19 2018-10-25 Electronic Biosciences, Inc. Nanopore/nanowell electrode enabled exonuclease sequencing
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CA3066715A1 (en) 2017-06-30 2019-01-03 Vib Vzw Novel protein pores
JP7042910B2 (ja) 2017-11-27 2022-03-28 エフ．ホフマン－ラ ロシュ アーゲー ナノポア－ｓｂｓ信号のための規格化およびベースラインシフト除去
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB202202716D0 (en) 2022-02-28 2022-04-13 Oxford Nanopore Tech Plc Apparatus and methods for controlling insertion of a membrane channel into a membrane
AU2023320396A1 (en) 2022-08-02 2025-02-20 Glyphic Biotechnologies, Inc. Protein sequencing via coupling of polymerizable molecules
GB202401864D0 (en) 2024-02-12 2024-03-27 Fund Centre De Regulacio Genòmica A method of detecting non-canonical bases in sequencing data

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE56026B1 (en) 1982-10-19 1991-03-27 Cetus Corp Cysteine-depleted muteins of biologically active proteins
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
WO1994025616A1 (en) 1993-04-28 1994-11-10 Worcester Foundation For Experimental Biology Cell-targeted lytic pore-forming agents
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型ｒｎａ−ｄｎａキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6267872B1 (en) * 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
JP2003502166A (ja) 1999-06-22 2003-01-21 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 固体状態の次元的特徴の制御
WO2001002425A2 (en) 1999-06-29 2001-01-11 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
WO2001040516A2 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
EP1255772A2 (de) * 2000-02-11 2002-11-13 The Texas A & M University System Zusammensetzungen für biosensoren und deren verwendung
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
WO2002042496A2 (en) 2000-11-27 2002-05-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
CA2448289A1 (en) * 2001-05-29 2002-12-19 Asahi Glass Company, Limited Method of constructing host and method of producing heterologous protein
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
AU2003245272A1 (en) 2002-05-10 2003-11-11 The Texas A And M University System Stochastic sensing through covalent interactions
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
CN101103357B (zh) 2004-08-13 2012-10-03 哈佛学院院长等 超高处理量光学-纳米孔dna读出平台
EP1842061A4 (de) 2004-12-21 2009-05-13 Texas A & M Univ Sys Hochtemperatur-ionenkanäle und ionenporen
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
CA2633476C (en) 2005-12-22 2015-04-21 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
WO2008045575A2 (en) 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
AU2008217579A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Formation of lipid bilayers
KR101521990B1 (ko) 2007-04-04 2015-05-20 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 나노포어 사용을 위한 조성물, 장치, 시스템 및 방법
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
CA2719747C (en) 2008-03-28 2018-02-20 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2010004273A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
DK2344891T3 (en) 2008-09-22 2016-06-06 Univ Washington MSP NANOPORES AND RELATED PROCEDURES

Also Published As

Publication number Publication date
AU2006314296A1 (en) 2007-05-24
CN101356288A (zh) 2009-01-28
JP2009519705A (ja) 2009-05-21
CN101356288B (zh) 2016-02-03
EP1951898A1 (de) 2008-08-06
EP2272981A1 (de) 2011-01-12
CA2629009A1 (en) 2007-05-24
NZ568163A (en) 2011-11-25
AU2006314296B2 (en) 2012-08-23
BRPI0618391A2 (pt) 2011-08-30
US8785211B2 (en) 2014-07-22
GB0523282D0 (en) 2005-12-21
IL191319A (en) 2011-10-31
US8105846B2 (en) 2012-01-31
EP2272981B1 (de) 2013-01-09
MY149161A (en) 2013-07-31
RU2008123842A (ru) 2009-12-27
UA95087C2 (en) 2011-07-11
WO2007057668A1 (en) 2007-05-24
US20080311582A1 (en) 2008-12-18
US20120219960A1 (en) 2012-08-30
JP5328362B2 (ja) 2013-10-30
KR20080083281A (ko) 2008-09-17
EP1951898B1 (de) 2012-02-15
CA2629009C (en) 2016-02-02

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