EA201591558A1 - Составы и способы увеличения производства рекомбинантного белка - Google Patents
Составы и способы увеличения производства рекомбинантного белкаInfo
- Publication number
- EA201591558A1 EA201591558A1 EA201591558A EA201591558A EA201591558A1 EA 201591558 A1 EA201591558 A1 EA 201591558A1 EA 201591558 A EA201591558 A EA 201591558A EA 201591558 A EA201591558 A EA 201591558A EA 201591558 A1 EA201591558 A1 EA 201591558A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- compositions
- methods
- increasing
- production
- recombinant protein
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title abstract 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 2
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 abstract 2
- 229910052791 calcium Inorganic materials 0.000 abstract 2
- 239000011575 calcium Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 239000013028 medium composition Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/14—Calcium; Ca chelators; Calcitonin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Описаны составы и способы увеличения производства рекомбинантных белков, а также другие аспекты. Указанные составы и способы относятся к увеличению содержания маннозы, или кальция, или обоих этих веществ в составе среды для культуры клеток для культивирования клеток, экспрессирующих рекомбинантные белки. В соответствии с некоторыми вариантами осуществления изобретения предоставляется состав среды для культур клеток млекопитающих, который содержит, по меньшей мере, одно из следующих веществ: маннозу приблизительно 3,5 г/л или более и кальций в диапазоне от приблизительно 1,5 до приблизительно 9,5 мМ. Также предоставляются различные другие аспекты и/или варианты осуществления изобретения.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361769402P | 2013-02-26 | 2013-02-26 | |
| PCT/US2014/018428 WO2014134071A1 (en) | 2013-02-26 | 2014-02-25 | Formulations and methods for increased recombinant protein production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA201591558A1 true EA201591558A1 (ru) | 2016-03-31 |
Family
ID=50240092
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201591558A EA201591558A1 (ru) | 2013-02-26 | 2014-02-25 | Составы и способы увеличения производства рекомбинантного белка |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US20160002592A1 (ru) |
| EP (1) | EP2961827A1 (ru) |
| JP (1) | JP2016508376A (ru) |
| KR (1) | KR20150121701A (ru) |
| CN (1) | CN105308174A (ru) |
| AR (1) | AR094875A1 (ru) |
| AU (1) | AU2014223680A1 (ru) |
| BR (1) | BR112015020253A2 (ru) |
| CA (1) | CA2902170A1 (ru) |
| EA (1) | EA201591558A1 (ru) |
| HK (1) | HK1220991A1 (ru) |
| IL (1) | IL240639A0 (ru) |
| MX (1) | MX2015010887A (ru) |
| PE (1) | PE20152019A1 (ru) |
| SG (1) | SG11201506212PA (ru) |
| TW (1) | TW201522634A (ru) |
| UY (1) | UY35343A (ru) |
| WO (1) | WO2014134071A1 (ru) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9481901B2 (en) | 2013-05-30 | 2016-11-01 | Amgen Inc. | Methods for increasing mannose content of recombinant proteins |
| EP3409783B1 (de) * | 2017-06-01 | 2020-12-16 | Justus-Liebig-Universität Gießen | Erfindung betreffend detektion und quantifizierung von colistin-resistenz bei gram-negativen bakterien |
| CN112006522B (zh) * | 2019-05-31 | 2022-01-28 | 佛山市顺德区美的电热电器制造有限公司 | 烹饪器具、用于烹饪器具的烹饪附件以及控制方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6238891B1 (en) * | 1987-11-18 | 2001-05-29 | Cetus Oncology Corporation | Method of increasing product expression through solute stress |
| CA2111561A1 (en) * | 1992-05-01 | 1993-11-11 | Yoshiharu Takazawa | Fed batch culture method for protein secreting cells |
| CA2595676A1 (en) * | 2005-01-25 | 2006-08-03 | Apollo Life Sciences Limited | Molecules and chimeric molecules thereof |
| CN101198621A (zh) * | 2005-01-25 | 2008-06-11 | 阿波罗生命科学有限公司 | 分子及其嵌合分子 |
| DE102005046225B4 (de) * | 2005-09-28 | 2012-01-05 | Cellca Gmbh | Verbessertes Zellkulturmedium |
| CA2682744C (en) * | 2007-04-16 | 2015-10-06 | Momenta Pharmaceuticals, Inc. | Methods related to cell surface glyosylation |
| GB0915481D0 (en) * | 2009-09-04 | 2009-10-07 | Arecor Ltd | Stable manufacture of factor V111 |
| US8969304B2 (en) * | 2010-04-09 | 2015-03-03 | The Institute For Cancer Research | Compositions and methods for the prevention of cancer in high risk patients |
| CA2804275A1 (en) * | 2010-07-08 | 2012-01-12 | Baxter International Inc. | Method of producing recombinant adamts13 in cell culture |
| US20130183280A1 (en) * | 2010-07-15 | 2013-07-18 | Novo Nordisk A/S | Stabilized factor viii variants |
-
2014
- 2014-02-21 UY UY0001035343A patent/UY35343A/es not_active Application Discontinuation
- 2014-02-24 TW TW103105992A patent/TW201522634A/zh unknown
- 2014-02-24 AR ARP140100579A patent/AR094875A1/es unknown
- 2014-02-25 HK HK16109114.4A patent/HK1220991A1/zh unknown
- 2014-02-25 EP EP14709147.4A patent/EP2961827A1/en not_active Withdrawn
- 2014-02-25 KR KR1020157022820A patent/KR20150121701A/ko not_active Withdrawn
- 2014-02-25 WO PCT/US2014/018428 patent/WO2014134071A1/en active Application Filing
- 2014-02-25 MX MX2015010887A patent/MX2015010887A/es unknown
- 2014-02-25 CA CA2902170A patent/CA2902170A1/en not_active Abandoned
- 2014-02-25 EA EA201591558A patent/EA201591558A1/ru unknown
- 2014-02-25 SG SG11201506212PA patent/SG11201506212PA/en unknown
- 2014-02-25 JP JP2015559286A patent/JP2016508376A/ja active Pending
- 2014-02-25 PE PE2015001799A patent/PE20152019A1/es not_active Application Discontinuation
- 2014-02-25 BR BR112015020253A patent/BR112015020253A2/pt not_active IP Right Cessation
- 2014-02-25 AU AU2014223680A patent/AU2014223680A1/en not_active Abandoned
- 2014-02-25 US US14/770,064 patent/US20160002592A1/en not_active Abandoned
- 2014-02-25 CN CN201480010579.8A patent/CN105308174A/zh active Pending
-
2015
- 2015-08-18 IL IL240639A patent/IL240639A0/en unknown
-
2017
- 2017-09-08 US US15/699,088 patent/US20180010090A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| TW201522634A (zh) | 2015-06-16 |
| WO2014134071A1 (en) | 2014-09-04 |
| CN105308174A (zh) | 2016-02-03 |
| MX2015010887A (es) | 2015-12-01 |
| US20180010090A1 (en) | 2018-01-11 |
| BR112015020253A2 (pt) | 2017-07-18 |
| US20160002592A1 (en) | 2016-01-07 |
| HK1220991A1 (zh) | 2017-05-19 |
| SG11201506212PA (en) | 2015-09-29 |
| AR094875A1 (es) | 2015-09-02 |
| AU2014223680A1 (en) | 2015-09-17 |
| EP2961827A1 (en) | 2016-01-06 |
| CA2902170A1 (en) | 2014-09-04 |
| UY35343A (es) | 2014-09-30 |
| PE20152019A1 (es) | 2016-01-29 |
| IL240639A0 (en) | 2015-09-24 |
| JP2016508376A (ja) | 2016-03-22 |
| KR20150121701A (ko) | 2015-10-29 |
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