DK3478832T3 - Fremgangsmåde til at screene for en mutant i en population af organismer ved at anvende en pooling- og splitting-tilgang - Google Patents

Fremgangsmåde til at screene for en mutant i en population af organismer ved at anvende en pooling- og splitting-tilgang Download PDF

Info

Publication number: DK3478832T3
Authority: DK; Denmark
Prior art keywords: pooling; mutant; organisms; population; screening
Prior art date: 2016-07-01

Application number

DK17733800.1T

Other languages

English (en)

Inventor

Toni Wendt

Hanne Cecilie Thomsen

Ole Olsen

Alexander Striebeck

Magnus Wohlfahrt Rasmussen

Massimiliano Carciofi

Søren Knudsen

Birgitte Skadhauge

Original Assignee

Carlsberg As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2016-07-01

Filing date

2017-06-23

Publication date

2022-05-30

2017-06-23 Application filed by Carlsberg As filed Critical Carlsberg As

2022-05-30 Application granted granted Critical

2022-05-30 Publication of DK3478832T3 publication Critical patent/DK3478832T3/da

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4624—Hordeum vulgarus [barley]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Biomedical Technology (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Chemical Kinetics & Catalysis (AREA)
Plant Pathology (AREA)
Crystallography & Structural Chemistry (AREA)
Developmental Biology & Embryology (AREA)
Botany (AREA)
Environmental Sciences (AREA)
Physiology (AREA)
Natural Medicines & Medicinal Plants (AREA)
Cell Biology (AREA)
General Chemical & Material Sciences (AREA)
Medicinal Chemistry (AREA)
Mycology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

DK17733800.1T 2016-07-01 2017-06-23 Fremgangsmåde til at screene for en mutant i en population af organismer ved at anvende en pooling- og splitting-tilgang DK3478832T3 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DKPA201670485		2016-07-01
PCT/EP2017/065516 WO2018001884A1 (en)	2016-07-01	2017-06-23	Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach

Publications (1)

Publication Number	Publication Date
DK3478832T3 true DK3478832T3 (da)	2022-05-30

Family

ID=56681913

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK17733800.1T DK3478832T3 (da)	2016-07-01	2017-06-23	Fremgangsmåde til at screene for en mutant i en population af organismer ved at anvende en pooling- og splitting-tilgang

Country Status (25)

Country	Link
US (2)	US11884972B2 (da)
EP (3)	EP3792357A1 (da)
JP (2)	JP7208797B2 (da)
CN (2)	CN114959098A (da)
AR (1)	AR110103A1 (da)
AU (3)	AU2017288960B2 (da)
BR (1)	BR112018076429A2 (da)
CA (1)	CA3026016A1 (da)
CL (1)	CL2018003710A1 (da)
CO (1)	CO2019000828A2 (da)
CR (1)	CR20190040A (da)
CU (1)	CU20180158A7 (da)
DK (1)	DK3478832T3 (da)
EA (1)	EA201892809A1 (da)
ES (1)	ES2916221T3 (da)
IL (1)	IL263451B (da)
MA (3)	MA45531A (da)
MX (2)	MX2018016110A (da)
PE (1)	PE20190228A1 (da)
PL (1)	PL3478832T3 (da)
PT (1)	PT3478832T (da)
SG (1)	SG11201811021YA (da)
UY (1)	UY37312A (da)
WO (1)	WO2018001884A1 (da)
ZA (3)	ZA201808218B (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
UA128692C2 (uk)	2017-12-28	2024-10-02	Карлсберг А/С	Зернова рослина з поліпшеними властивостями клітинної стінки
CN108913698B (zh) *	2018-07-25	2021-10-26	安徽农业大学	一种与小麦穗发芽抗性/感性相关的caps标记及其应用
CN110724741B (zh) *	2019-07-24	2020-05-19	艾普拜生物科技(苏州)有限公司	一种检测微小残留白血病相关融合基因的引物、探针及其试剂盒
EP3785529A1 (en)	2019-08-28	2021-03-03	Carlsberg A/S	Barley plants with altered protein content in grains
JP2023502317A (ja)	2019-10-10	2023-01-24	カールスバーグアグシャセルスガーブ	変異体植物の調製方法
CN115297717B (zh)	2020-03-02	2024-05-17	嘉士伯有限公司	具有高极限糊精酶活性的大麦植物
EP4029935A1 (en)	2021-01-14	2022-07-20	Carlsberg A/S	S. eubayanus strain and hybrids thereof
EP4423297A1 (en) *	2021-10-25	2024-09-04	GT Molecular, Inc.	Off-target blocking sequences to improve target discrimination by polymerase chain reaction
EP4451853A1 (en)	2021-12-22	2024-10-30	Basf Agricultural Solutions Seed Us Llc	Improved screening method for genome edited events
EP4490736A1 (en)	2022-03-11	2025-01-15	Carlsberg A/S	Modulation of protein levels
WO2024105148A1 (en)	2022-11-16	2024-05-23	University Of Copenhagen	Plants with low seed glucosinolate content
WO2024133900A1 (en)	2022-12-22	2024-06-27	University Of Copenhagen	Improved (-)-sparteine production
WO2024227748A1 (en)	2023-05-01	2024-11-07	Carlsberg A/S	Barley with improved properties
CN117965576B (zh) *	2024-02-01	2024-08-27	北京林业大学	一种王百合苷生物合成基因LlHCT1及其应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3996345A (en)	1974-08-12	1976-12-07	Syva Company	Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en)	1979-09-07	1982-09-28	Syva Company	Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US5188934A (en)	1989-11-14	1993-02-23	Applied Biosystems, Inc.	4,7-dichlorofluorescein dyes as molecular probes
JP2001507570A (ja)	1996-11-26	2001-06-12	バイラー・カレッジ・オブ・メディスン	染色体末端へのテロメラーゼのアクセスを阻害する阻害剤の同定
US6187544B1 (en)	1997-06-04	2001-02-13	Smithkline Beecham Corporation	Methods for rapid cloning for full length cDNAs using a pooling strategy
WO1999051774A2 (en)	1998-04-02	1999-10-14	Tellus Genetic Resources, Inc.	A method for obtaining a plant with a genetic lesion in a gene sequence
GB9812768D0 (en)	1998-06-13	1998-08-12	Zeneca Ltd	Methods
WO2003000905A2 (en) *	2001-06-22	2003-01-03	Syngenta Participations Ag	Identification and characterization of plant genes
GB0127564D0 (en)	2001-11-16	2002-01-09	Medical Res Council	Emulsion compositions
US7420105B2 (en)	2004-03-11	2008-09-02	Carlsberg A/S	Barley for production of flavor-stable beverage
EP4512526A2 (en)	2008-09-23	2025-02-26	Bio-Rad Laboratories, Inc.	Droplet-based assay system
US9005565B2 (en)	2010-06-24	2015-04-14	Hamid-Reza Jahangiri-Famenini	Method and apparatus for forming graphene
WO2012122571A1 (en) *	2011-03-10	2012-09-13	Gen-Probe Incorporated	Methods and compositions for the selection and optimization of oligonucleotide tag sequences
US9017979B2 (en)	2011-04-11	2015-04-28	Roche Molecular Systems, Inc.	DNA polymerases with improved activity
ES2898460T3 (es)	2014-02-27	2022-03-07	Monsanto Technology Llc	Composiciones y procedimientos para la modificación genómica dirigida al sitio
GB201411603D0 (en)	2014-06-30	2014-08-13	Vela Operations Pte Ltd	Compositions for quantitative and/or semiquantitative mutation detection methods

2017
- 2017-06-23 EP EP20200115.2A patent/EP3792357A1/en active Pending
- 2017-06-23 US US16/311,450 patent/US11884972B2/en active Active
- 2017-06-23 MA MA045531A patent/MA45531A/fr unknown
- 2017-06-23 WO PCT/EP2017/065516 patent/WO2018001884A1/en active Application Filing
- 2017-06-23 CR CR20190040A patent/CR20190040A/es unknown
- 2017-06-23 MA MA054568A patent/MA54568A/fr unknown
- 2017-06-23 ES ES17733800T patent/ES2916221T3/es active Active
- 2017-06-23 EP EP17733800.1A patent/EP3478832B1/en active Active
- 2017-06-23 PL PL17733800T patent/PL3478832T3/pl unknown
- 2017-06-23 CN CN202210633145.XA patent/CN114959098A/zh active Pending
- 2017-06-23 PE PE2018003333A patent/PE20190228A1/es unknown
- 2017-06-23 EP EP20163553.9A patent/EP3693458A1/en active Pending
- 2017-06-23 PT PT177338001T patent/PT3478832T/pt unknown
- 2017-06-23 SG SG11201811021YA patent/SG11201811021YA/en unknown
- 2017-06-23 AU AU2017288960A patent/AU2017288960B2/en active Active
- 2017-06-23 BR BR112018076429A patent/BR112018076429A2/pt unknown
- 2017-06-23 JP JP2018569004A patent/JP7208797B2/ja active Active
- 2017-06-23 MX MX2018016110A patent/MX2018016110A/es unknown
- 2017-06-23 CA CA3026016A patent/CA3026016A1/en active Pending
- 2017-06-23 MA MA052594A patent/MA52594A/fr unknown
- 2017-06-23 CU CU2018000158A patent/CU20180158A7/es unknown
- 2017-06-23 EA EA201892809A patent/EA201892809A1/ru unknown
- 2017-06-23 CN CN201780041101.5A patent/CN109477087B/zh active Active
- 2017-06-23 DK DK17733800.1T patent/DK3478832T3/da active
- 2017-06-23 IL IL263451A patent/IL263451B/en unknown
- 2017-06-30 AR ARP170101817A patent/AR110103A1/es unknown
- 2017-06-30 UY UY0001037312A patent/UY37312A/es not_active Application Discontinuation
2018
- 2018-12-05 ZA ZA2018/08218A patent/ZA201808218B/en unknown
- 2018-12-18 MX MX2022014965A patent/MX2022014965A/es unknown
- 2018-12-20 CL CL2018003710A patent/CL2018003710A1/es unknown
2019
- 2019-01-29 CO CONC2019/0000828A patent/CO2019000828A2/es unknown
- 2019-11-20 AU AU2019268103A patent/AU2019268103B2/en active Active
- 2019-11-20 AU AU2019268102A patent/AU2019268102B2/en active Active
- 2019-11-25 ZA ZA2019/07775A patent/ZA201907775B/en unknown
- 2019-11-25 ZA ZA2019/07774A patent/ZA201907774B/en unknown
2022
- 2022-05-06 JP JP2022076471A patent/JP7539428B2/ja active Active
2023
- 2023-12-12 US US18/536,784 patent/US20240182961A1/en active Pending

Also Published As

Publication number	Publication date
AU2019268103A1 (en)	2019-12-12
CR20190040A (es)	2019-05-09
CO2019000828A2 (es)	2019-04-30
ZA201907775B (en)	2022-09-28
AR110103A1 (es)	2019-02-27
CU20180158A7 (es)	2019-08-06
CL2018003710A1 (es)	2019-03-22
CN109477087B (zh)	2022-06-24
CN109477087A (zh)	2019-03-15
AU2017288960A1 (en)	2019-01-17
ZA201808218B (en)	2020-02-26
AU2019268102A1 (en)	2019-12-12
US11884972B2 (en)	2024-01-30
US20240182961A1 (en)	2024-06-06
MA54568A (fr)	2021-10-27
EA201892809A1 (ru)	2019-06-28
MX2022014965A (es)	2023-01-11
JP2019524083A (ja)	2019-09-05
WO2018001884A1 (en)	2018-01-04
MX2018016110A (es)	2019-05-30
PL3478832T3 (pl)	2022-06-06
US20190194723A1 (en)	2019-06-27
SG11201811021YA (en)	2019-01-30
CA3026016A1 (en)	2018-01-04
AU2019268102B2 (en)	2020-03-05
PT3478832T (pt)	2022-05-19
EP3478832A1 (en)	2019-05-08
IL263451B (en)	2022-08-01
AU2019268103B2 (en)	2020-03-12
UY37312A (es)	2018-02-28
JP7208797B2 (ja)	2023-01-19
EP3792357A1 (en)	2021-03-17
MA45531A (fr)	2019-05-08
ZA201907774B (en)	2022-07-27
EP3478832B1 (en)	2022-02-23
PE20190228A1 (es)	2019-02-13
MA52594A (fr)	2021-03-17
JP2022101703A (ja)	2022-07-06
ES2916221T3 (es)	2022-06-29
IL263451A (en)	2019-01-31
EP3693458A1 (en)	2020-08-12
AU2017288960B2 (en)	2019-12-12
JP7539428B2 (ja)	2024-08-23
BR112018076429A2 (pt)	2019-10-01
CN114959098A (zh)	2022-08-30

Publication	Publication Date	Title
DK3478832T3 (da)	2022-05-30	Fremgangsmåde til at screene for en mutant i en population af organismer ved at anvende en pooling- og splitting-tilgang
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DK3548033T3 (da)	2024-07-15	Forbindelser og deres fremgangsmåde til anvendelse
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