[go: up one dir, main page]

EA201892809A1 - Способ скрининга мутанта в популяции организмов с применением подхода объединения в пулы и разделения - Google Patents

Способ скрининга мутанта в популяции организмов с применением подхода объединения в пулы и разделения

Info

Publication number
EA201892809A1
EA201892809A1 EA201892809A EA201892809A EA201892809A1 EA 201892809 A1 EA201892809 A1 EA 201892809A1 EA 201892809 A EA201892809 A EA 201892809A EA 201892809 A EA201892809 A EA 201892809A EA 201892809 A1 EA201892809 A1 EA 201892809A1
Authority
EA
Eurasian Patent Office
Prior art keywords
organisms
dpcr
pools
population
association
Prior art date
Application number
EA201892809A
Other languages
English (en)
Inventor
Тони Вендт
Оле Олсен
Сорен Кнудсен
Ханне Сесил Томсен
Александр Страйбек
Биргитт Скадхауг
Магнус Вольфарт Расмуссен
Массимилиано Карчофи
Original Assignee
Карлсберг А/С
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Карлсберг А/С filed Critical Карлсберг А/С
Publication of EA201892809A1 publication Critical patent/EA201892809A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4624—Hordeum vulgarus [barley]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

В традиционных подходах к селекции растений химический мутагенез можно использовать для случайного введения нуклеотидных замен в геном растения, т.е. без возможности контролировать сайты нуклеотидных изменений. Когда дело касается поиска заданной нуклеотидной замены, статистическая вероятность является крайне низкой из-за сложности генома. В настоящем изобретении, тем не менее, продемонстрировано, как разработать новое альтернативное применение цифровой полимеразной цепной реакции (dPCR), предпочтительно капельной dPCR (ddPCR), для обнаружения конкретных нуклеотидных замен в мутантных генах. Вся платформа включает способ скрининга с библиотекой подвергнутых мутагенезу организмов, системы на основе цифровой ПЦР и установку для размножения и анализа выявленных мутантных организмов.
EA201892809A 2016-07-01 2017-06-23 Способ скрининга мутанта в популяции организмов с применением подхода объединения в пулы и разделения EA201892809A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201670485 2016-07-01
PCT/EP2017/065516 WO2018001884A1 (en) 2016-07-01 2017-06-23 Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach

Publications (1)

Publication Number Publication Date
EA201892809A1 true EA201892809A1 (ru) 2019-06-28

Family

ID=56681913

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201892809A EA201892809A1 (ru) 2016-07-01 2017-06-23 Способ скрининга мутанта в популяции организмов с применением подхода объединения в пулы и разделения

Country Status (25)

Country Link
US (2) US11884972B2 (ru)
EP (3) EP3792357B1 (ru)
JP (2) JP7208797B2 (ru)
CN (2) CN114959098A (ru)
AR (1) AR110103A1 (ru)
AU (3) AU2017288960B2 (ru)
BR (1) BR112018076429A2 (ru)
CA (1) CA3026016A1 (ru)
CL (1) CL2018003710A1 (ru)
CO (1) CO2019000828A2 (ru)
CR (1) CR20190040A (ru)
CU (1) CU20180158A7 (ru)
DK (1) DK3478832T3 (ru)
EA (1) EA201892809A1 (ru)
ES (1) ES2916221T3 (ru)
IL (1) IL263451B (ru)
MA (3) MA54568A (ru)
MX (2) MX2018016110A (ru)
PE (1) PE20190228A1 (ru)
PL (2) PL3478832T3 (ru)
PT (1) PT3478832T (ru)
SG (1) SG11201811021YA (ru)
UY (1) UY37312A (ru)
WO (1) WO2018001884A1 (ru)
ZA (3) ZA201808218B (ru)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA54568A (fr) * 2016-07-01 2021-10-27 Carlsberg As Substitutions de nucléotides prédéterminées
US11690341B2 (en) 2017-12-28 2023-07-04 Carlsberg A/S Cereal plants with improved cell wall properties
CA3106307A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
US20220362771A1 (en) * 2018-07-13 2022-11-17 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
CN108913698B (zh) * 2018-07-25 2021-10-26 安徽农业大学 一种与小麦穗发芽抗性/感性相关的caps标记及其应用
CN110724741B (zh) * 2019-07-24 2020-05-19 艾普拜生物科技(苏州)有限公司 一种检测微小残留白血病相关融合基因的引物、探针及其试剂盒
EP4013843A1 (en) * 2019-08-16 2022-06-22 Heineken Supply Chain B.V. Production of an alcohol-free beverage
EP3785529A1 (en) 2019-08-28 2021-03-03 Carlsberg A/S Barley plants with altered protein content in grains
CN114786473B (zh) * 2019-10-10 2023-12-19 嘉士伯有限公司 制备突变植物的方法
WO2021175786A1 (en) 2020-03-02 2021-09-10 Carlsberg A/S Barley plants with high limit dextrinase activity
EP4029935A1 (en) 2021-01-14 2022-07-20 Carlsberg A/S S. eubayanus strain and hybrids thereof
JP2024538973A (ja) * 2021-10-25 2024-10-28 ジーティー モレキュラー インコーポレイテッド ポリメラーゼ連鎖反応によるターゲット識別を向上させるオフターゲットブロック配列
EP4451853A1 (en) 2021-12-22 2024-10-30 Basf Agricultural Solutions Seed Us Llc Improved screening method for genome edited events
EP4490736A1 (en) 2022-03-11 2025-01-15 Carlsberg A/S Modulation of protein levels
WO2024105148A1 (en) 2022-11-16 2024-05-23 University Of Copenhagen Plants with low seed glucosinolate content
WO2024133900A1 (en) 2022-12-22 2024-06-27 University Of Copenhagen Improved (-)-sparteine production
WO2024227748A1 (en) 2023-05-01 2024-11-07 Carlsberg A/S Barley with improved properties
WO2025108936A1 (en) * 2023-11-20 2025-05-30 Eth Zurich Method for providing genetically-modified cells
CN117965576B (zh) * 2024-02-01 2024-08-27 北京林业大学 一种王百合苷生物合成基因LlHCT1及其应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
WO1998023759A2 (en) 1996-11-26 1998-06-04 Baylor College Of Medicine Identification of inhibitors that prevent access of telomerase to chromosomal terminus
US6187544B1 (en) 1997-06-04 2001-02-13 Smithkline Beecham Corporation Methods for rapid cloning for full length cDNAs using a pooling strategy
WO1999051774A2 (en) 1998-04-02 1999-10-14 Tellus Genetic Resources, Inc. A method for obtaining a plant with a genetic lesion in a gene sequence
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
WO2003000905A2 (en) * 2001-06-22 2003-01-03 Syngenta Participations Ag Identification and characterization of plant genes
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7420105B2 (en) 2004-03-11 2008-09-02 Carlsberg A/S Barley for production of flavor-stable beverage
AU2009297108B2 (en) 2008-09-23 2015-02-12 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9005565B2 (en) 2010-06-24 2015-04-14 Hamid-Reza Jahangiri-Famenini Method and apparatus for forming graphene
EP3498864B1 (en) * 2011-03-10 2024-10-09 Gen-Probe Incorporated Methods for the selection and optimization of oligonucleotide tag sequences
US9017979B2 (en) 2011-04-11 2015-04-28 Roche Molecular Systems, Inc. DNA polymerases with improved activity
CA2940217C (en) 2014-02-27 2023-06-13 Monsanto Technology Llc Compositions and methods for site directed genomic modification
GB201411603D0 (en) 2014-06-30 2014-08-13 Vela Operations Pte Ltd Compositions for quantitative and/or semiquantitative mutation detection methods
MA54568A (fr) * 2016-07-01 2021-10-27 Carlsberg As Substitutions de nucléotides prédéterminées
CN114786473B (zh) * 2019-10-10 2023-12-19 嘉士伯有限公司 制备突变植物的方法

Also Published As

Publication number Publication date
CA3026016A1 (en) 2018-01-04
MX2018016110A (es) 2019-05-30
PL3792357T3 (pl) 2025-07-07
EP3478832B1 (en) 2022-02-23
CN109477087B (zh) 2022-06-24
AR110103A1 (es) 2019-02-27
MX2022014965A (es) 2023-01-11
CN109477087A (zh) 2019-03-15
MA52594A (fr) 2021-03-17
DK3478832T3 (da) 2022-05-30
IL263451B (en) 2022-08-01
JP7539428B2 (ja) 2024-08-23
EP3693458A1 (en) 2020-08-12
AU2017288960A1 (en) 2019-01-17
EP3478832A1 (en) 2019-05-08
MA54568A (fr) 2021-10-27
US20240182961A1 (en) 2024-06-06
AU2019268102B2 (en) 2020-03-05
BR112018076429A2 (pt) 2019-10-01
AU2019268103A1 (en) 2019-12-12
UY37312A (es) 2018-02-28
ZA201907775B (en) 2022-09-28
CR20190040A (es) 2019-05-09
PE20190228A1 (es) 2019-02-13
IL263451A (en) 2019-01-31
AU2019268103B2 (en) 2020-03-12
PT3478832T (pt) 2022-05-19
JP2019524083A (ja) 2019-09-05
SG11201811021YA (en) 2019-01-30
EP3792357C0 (en) 2025-05-07
CN114959098A (zh) 2022-08-30
WO2018001884A1 (en) 2018-01-04
CO2019000828A2 (es) 2019-04-30
AU2017288960B2 (en) 2019-12-12
CL2018003710A1 (es) 2019-03-22
AU2019268102A1 (en) 2019-12-12
CU20180158A7 (es) 2019-08-06
US20190194723A1 (en) 2019-06-27
JP2022101703A (ja) 2022-07-06
EP3792357A1 (en) 2021-03-17
EP3792357B1 (en) 2025-05-07
US11884972B2 (en) 2024-01-30
ES2916221T3 (es) 2022-06-29
ZA201907774B (en) 2022-07-27
ZA201808218B (en) 2020-02-26
MA45531A (fr) 2019-05-08
JP7208797B2 (ja) 2023-01-19
PL3478832T3 (pl) 2022-06-06

Similar Documents

Publication Publication Date Title
EA201892809A1 (ru) Способ скрининга мутанта в популяции организмов с применением подхода объединения в пулы и разделения
Tedersoo et al. Perspectives and benefits of high-throughput long-read sequencing in microbial ecology
Sze et al. The impact of DNA polymerase and number of rounds of amplification in PCR on 16S rRNA gene sequence data
Redman et al. The emergence of resistance to the benzimidazole anthlemintics in parasitic nematodes of livestock is characterised by multiple independent hard and soft selective sweeps
McDaniel et al. Expanded phylogenetic diversity and metabolic flexibility of mercury-methylating microorganisms
Brown et al. Unusual biology across a group comprising more than 15% of domain Bacteria
Skvortsov et al. Metagenomic characterisation of the viral community of Lough Neagh, the largest freshwater lake in Ireland
Shinzato et al. A snapshot of a coral “holobiont”: a transcriptome assembly of the scleractinian coral, Porites, captures a wide variety of genes from both the host and symbiotic zooxanthellae
Porazinska et al. Reproducibility of read numbers in high‐throughput sequencing analysis of nematode community composition and structure
Auffret et al. The role of microbial community composition in controlling soil respiration responses to temperature
MX2024001256A (es) Metodos para la deteccion de cambios de copia genomica en muestras de adn.
EP4467654A3 (en) Genome fractioning
Song et al. Effort versus reward: preparing samples for fungal community characterization in high-throughput sequencing surveys of soils
Kono et al. Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly
MX2018015412A (es) Sistema y metodo para analisis secundario de datos de secuenciamiento de nucleotido.
Albright et al. Biotic interactions are more important than propagule pressure in microbial community invasions
Wibberg et al. Development of a Rhizoctonia solani AG1-IB specific gene model enables comparative genome analyses between phytopathogenic R. solani AG1-IA, AG1-IB, AG3 and AG8 isolates
Dufresnes et al. Stronger transferability but lower variability in transcriptomic‐than in anonymous microsatellites: evidence from H ylid frogs
TR201903963T4 (tr) Hizalama içermeyen sekans analizi kullanılarak bir hedef sekans içindeki mutasyon(lar)ı içeren üyeler için bir popülasyonun taranmasına ait yüksek verimlilik metodu.
Suez et al. MicNeSs: genotyping microsatellite loci from a collection of (NGS) reads
Keča et al. Initial characterization of an unidentified A rmillaria isolate from S erbia using LSU‐IGS 1 and TEF‐1‐α genes
Leontidou et al. Simultaneous analysis of seven 16S rRNA hypervariable gene regions increases efficiency in marine bacterial diversity detection
Keeling Bark beetle research in the postgenomic era
González‐Tortuero et al. The Quantification of Representative Sequences pipeline for amplicon sequencing: case study on within‐population ITS 1 sequence variation in a microparasite infecting D aphnia
Robertson et al. Genome sequences of key bacterial symbionts of entomopathogenic nematodes: Xenorhabdus cabanillasii DSM17905, Xenorhabdus ehlersii DSM16337, Xenorhabdus japonica DSM16522, Xenorhabdus koppenhoeferii DSM18168, and Xenorhabdus mauleonii DSM17908