[go: up one dir, main page]

DE69634406D1 - Verfahren zum in vitro testen der proteolytischen aktivität von polypeptiden mit hcv ns3 protease aktivität sowie peptidsubstrate, die in diesem verfahren verwendet werden - Google Patents

Verfahren zum in vitro testen der proteolytischen aktivität von polypeptiden mit hcv ns3 protease aktivität sowie peptidsubstrate, die in diesem verfahren verwendet werden

Info

Publication number
DE69634406D1
DE69634406D1 DE69634406T DE69634406T DE69634406D1 DE 69634406 D1 DE69634406 D1 DE 69634406D1 DE 69634406 T DE69634406 T DE 69634406T DE 69634406 T DE69634406 T DE 69634406T DE 69634406 D1 DE69634406 D1 DE 69634406D1
Authority
DE
Germany
Prior art keywords
hcv
polypeptides
protease
proteolytic activity
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
DE69634406T
Other languages
English (en)
Other versions
DE69634406T2 (de
Inventor
Christian Steinkuehler
Antonello Pessi
Elisabetta Bianchi
Marina Taliani
Licia Tomei
Andrea Urbani
Francesco Raffaele De
Frank Narjes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Istituto di Ricerche di Biologia Molecolare P Angeletti SpA
Original Assignee
Istituto di Ricerche di Biologia Molecolare P Angeletti SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=11403536&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69634406(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Istituto di Ricerche di Biologia Molecolare P Angeletti SpA filed Critical Istituto di Ricerche di Biologia Molecolare P Angeletti SpA
Application granted granted Critical
Publication of DE69634406D1 publication Critical patent/DE69634406D1/de
Publication of DE69634406T2 publication Critical patent/DE69634406T2/de
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DE69634406T 1995-08-22 1996-08-20 Verfahren zum In-Vitro-Texten der proteolytischen Aktivität von Polypeptiden mit HCV NS3 Protease-Aktivität sowie Peptidsubstrate, die in diesem Verfahren verwendet werden Revoked DE69634406T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IT95RM000573A IT1277914B1 (it) 1995-08-22 1995-08-22 Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e
ITRM950573 1995-08-22
PCT/IT1996/000163 WO1997008304A2 (en) 1995-08-22 1996-08-20 Methodology to produce, purify and assay polypeptides with the proteolytic activity of the hcv ns3 protease

Publications (2)

Publication Number Publication Date
DE69634406D1 true DE69634406D1 (de) 2005-04-07
DE69634406T2 DE69634406T2 (de) 2006-04-13

Family

ID=11403536

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69634406T Revoked DE69634406T2 (de) 1995-08-22 1996-08-20 Verfahren zum In-Vitro-Texten der proteolytischen Aktivität von Polypeptiden mit HCV NS3 Protease-Aktivität sowie Peptidsubstrate, die in diesem Verfahren verwendet werden

Country Status (14)

Country Link
US (1) US6197536B1 (de)
EP (1) EP0846164B1 (de)
JP (1) JP3310298B2 (de)
CN (1) CN1193997A (de)
AT (1) ATE290070T1 (de)
AU (1) AU716379B2 (de)
BR (1) BR9610039A (de)
CA (1) CA2228265C (de)
DE (1) DE69634406T2 (de)
DK (1) DK0846164T3 (de)
ES (1) ES2236743T3 (de)
IT (1) IT1277914B1 (de)
PT (1) PT846164E (de)
WO (1) WO1997008304A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1285158B1 (it) * 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
GB9623908D0 (en) * 1996-11-18 1997-01-08 Hoffmann La Roche Amino acid derivatives
GB9707659D0 (en) * 1997-04-16 1997-06-04 Peptide Therapeutics Ltd Hepatitis C NS3 Protease inhibitors
ES2143918B1 (es) * 1997-05-23 2001-01-01 Inst Cientifico Tecnol Navarra Peptidos inhibidores de la proteasa c y su utilizacion en la preparacion de composiciones utiles en las infecciones causadas por el virus de la hepatitis c.
US6211338B1 (en) 1997-11-28 2001-04-03 Schering Corporation Single-chain recombinant complexes of hepatitis C virus NS3 protease and NS4A cofactor peptide
IT1299134B1 (it) * 1998-02-02 2000-02-29 Angeletti P Ist Richerche Bio Procedimento per la produzione di peptidi con proprieta' inibitrici della proteasi ns3 del virus hcv, peptidi cosi' ottenibili e peptidi
AU4855299A (en) * 1998-07-02 2000-01-24 University Of Florida Ns4a-ns3 catalytic domain of hepatitis c
US6323180B1 (en) * 1998-08-10 2001-11-27 Boehringer Ingelheim (Canada) Ltd Hepatitis C inhibitor tri-peptides
JP2002534084A (ja) 1999-01-08 2002-10-15 ブリストル−マイヤーズ スクイブ カンパニー Ｃ型肝炎ウイルスｎｓ３プロテアーゼの修飾形
IL145376A0 (en) * 1999-05-04 2002-06-30 Boehringer Ingelheim Ca Ltd Surrogate cell-based system and method for assaying the activity of hepatitis c virus ns3 protease
EP1214454A1 (de) * 1999-08-30 2002-06-19 Merck & Co., Inc. Inhibitoren der hepatitis c virus replikation
GB0006537D0 (en) * 2000-03-17 2000-05-10 Angeletti P Ist Richerche Bio Assays and screening methods
DE60127299T2 (de) 2000-12-15 2007-08-30 Boehringer Ingelheim (Canada) Ltd., Laval Gereinigte aktive hcv ns2/3 protease
ATE385253T1 (de) * 2002-10-29 2008-02-15 Boehringer Ingelheim Int Nhibitor-resistente hcv ns3 protease
WO2006102087A2 (en) * 2005-03-22 2006-09-28 Merck & Co., Inc. Hcv protease substrates
CA2627247C (en) * 2005-10-28 2013-04-02 Boehringer Ingelheim International Gmbh Hepatitis c virus ns2/3 activity assay
CN103012565B (zh) * 2013-01-15 2014-07-16 成都天台山制药有限公司 一种曲普瑞林及其固相合成制备方法
JP6564539B1 (ja) 2018-09-14 2019-08-21 長瀬産業株式会社 スルホン酸化合物によるペプチド精製方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69133402T2 (de) * 1990-04-04 2004-11-11 Chiron Corp. (N.D.Ges.D. Staates Delaware), Emeryville Protease von hepatitis-c-virus
EP0729973A4 (de) * 1993-10-29 1998-12-30 Srl Inc Eine antigene peptidverbindung und ein immunoassay-verfahren
JPH08205893A (ja) * 1994-12-08 1996-08-13 Sumitomo Metal Ind Ltd Ｈｃｖプロテアーゼ活性測定法
US5767233A (en) * 1995-05-12 1998-06-16 Schering Corporation Soluble cleavable substrates of the hepatitis C virus protease

Also Published As

Publication number Publication date
ES2236743T3 (es) 2005-07-16
DE69634406T2 (de) 2006-04-13
EP0846164A2 (de) 1998-06-10
JP3310298B2 (ja) 2002-08-05
ITRM950573A0 (it) 1995-08-22
CA2228265C (en) 2002-08-13
PT846164E (pt) 2005-07-29
CN1193997A (zh) 1998-09-23
EP0846164B1 (de) 2005-03-02
JPH10511556A (ja) 1998-11-10
US6197536B1 (en) 2001-03-06
IT1277914B1 (it) 1997-11-12
ATE290070T1 (de) 2005-03-15
AU716379B2 (en) 2000-02-24
DK0846164T3 (da) 2005-06-27
CA2228265A1 (en) 1997-03-06
BR9610039A (pt) 1999-07-06
ITRM950573A1 (it) 1997-02-22
WO1997008304A2 (en) 1997-03-06
WO1997008304A3 (en) 1997-05-22
AU6668696A (en) 1997-03-19

Similar Documents

Publication Publication Date Title
DE69634406D1 (de) Verfahren zum in vitro testen der proteolytischen aktivität von polypeptiden mit hcv ns3 protease aktivität sowie peptidsubstrate, die in diesem verfahren verwendet werden
Yagel et al. Suppression by cathepsin L inhibitors of the invasion of amnion membranes by murine cancer cells
Reich et al. Effects of inhibitors of plasminogen activator, serine proteinases, and collagenase IV on the invasion of basement membranes by metastatic cells
Várallyay et al. Two mutations in rat trypsin confer resistance against autolysis
Beers et al. Ovarian plasminogen activator: relationship to ovulation and hormonal regulation
CA2182521A1 (en) Method for Reproducing in Vitro the Proteolytic Activity of the NS3 Protease of Hepatitis C Virus (HCV)
Takeya et al. Primary Structures of Platelet Aggregation Inhibitors (Disintegrinis) Autoproteolytically Released from Snake Venom Hemorrhagic Metalloproteinases and New Flurogenic Peptide Subtrates for These Enzymes
Stoecker et al. Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease
Lee et al. Atomic structure of the trypsin-cyclotheonamide A complex: lessons for the design of serine protease inhibitors
RU96119350A (ru) Способ репродукции in vitro протеолитической активности ns3 протеазы вируса гепатита c(нсу)
ES477501A1 (es) Procedimiento para la obtencion de substratos enzimaticos.
Gibson et al. Chymotrypsin-like proteases from the sea anemone, Metridium senile
Burgos et al. Analysis of the activation process of porcine procarboxypeptidase B and determination of the sequence of its activation segment
Harris A collagenolytic system produced by primary cultures of rheumatoid nodule tissue
Brezniak et al. Human. alpha.-to. zeta.-thrombin cleavage occurs with neutrophil cathepsin G or chymotrypsin while fibrinogen clotting activity is retained
Bózner et al. Degradation of collagen types I, III, IV and V by extracellular proteinases of an oral flagellate Trichomonas tenax
GR3025330T3 (en) Variants of pai-2
Sawada et al. Substrate specificity of ascidian sperm trypsin‐like proteases, spermosin and acrosin
ES2060202T5 (es) Composicion detergente enzimatica liquida.
Berg et al. Formation of desTyr dynorphins 5–17 by a purified cytosolic aminopeptidase of rat brain
Marossy et al. Mapping of the substrate-binding site of the human granulocyte elastase by the aid of tripeptidyl-p-nitroanilide substrates
Taylor et al. Structure and function of bovine lens aminopeptidase and comparison with homologous aminopeptidases
Chiravuri et al. Aminodipeptidase inhibitor-induced cell death in quiescent lymphocytes: a review
JPS6414000A (en) Testing agent for periodontosis
Turner et al. Pharmacology of enkephalinase inhibitors

Legal Events

Date Code Title Description
8363 Opposition against the patent
8331 Complete revocation