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DE60230788D1 - Isolierung und aufreinigung von nukleinsäuren - Google Patents

Isolierung und aufreinigung von nukleinsäuren

Info

Publication number
DE60230788D1
DE60230788D1 DE60230788T DE60230788T DE60230788D1 DE 60230788 D1 DE60230788 D1 DE 60230788D1 DE 60230788 T DE60230788 T DE 60230788T DE 60230788 T DE60230788 T DE 60230788T DE 60230788 D1 DE60230788 D1 DE 60230788D1
Authority
DE
Germany
Prior art keywords
nucleic acids
isolation
purification
paper
cellulose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60230788T
Other languages
English (en)
Inventor
R D Nargessi
Matt Pourfarzaneh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Cortex Biochem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cortex Biochem Inc filed Critical Cortex Biochem Inc
Application granted granted Critical
Publication of DE60230788D1 publication Critical patent/DE60230788D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60230788T 2001-11-06 2002-09-12 Isolierung und aufreinigung von nukleinsäuren Expired - Lifetime DE60230788D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33790401P 2001-11-06 2001-11-06
PCT/US2002/029378 WO2003040687A2 (en) 2001-11-06 2002-09-12 Isolation and purification of nucleic acids

Publications (1)

Publication Number Publication Date
DE60230788D1 true DE60230788D1 (de) 2009-02-26

Family

ID=23322508

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60238648T Expired - Lifetime DE60238648D1 (de) 2001-11-06 2002-09-12 Isolierung und Reinigung von Nucleinsäuren
DE60230788T Expired - Lifetime DE60230788D1 (de) 2001-11-06 2002-09-12 Isolierung und aufreinigung von nukleinsäuren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60238648T Expired - Lifetime DE60238648D1 (de) 2001-11-06 2002-09-12 Isolierung und Reinigung von Nucleinsäuren

Country Status (10)

Country Link
US (1) US7264927B2 (de)
EP (3) EP2258845B1 (de)
JP (2) JP4277115B2 (de)
AT (2) ATE491790T1 (de)
AU (1) AU2002333673B2 (de)
CA (1) CA2465961A1 (de)
DE (2) DE60238648D1 (de)
DK (1) DK1442045T3 (de)
ES (1) ES2321492T3 (de)
WO (1) WO2003040687A2 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040137449A1 (en) * 2001-10-05 2004-07-15 Nargessi R D Magnetic isolation and purification of nucleic acids
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
WO2003040687A2 (en) * 2001-11-06 2003-05-15 Cortex Biochem, Inc. Isolation and purification of nucleic acids
JP3983125B2 (ja) * 2002-07-19 2007-09-26 富士フイルム株式会社 核酸の分離精製方法
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
WO2005068662A1 (en) * 2003-12-30 2005-07-28 Sigma-Aldrich Co. Rapid preparation of nucleic acids by enzymatic digestion
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
CN101124321B (zh) 2004-11-05 2012-02-29 恰根北美控股有限公司 用于从稳定剂中纯化核酸的组合物和方法
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20060223073A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
WO2007140417A2 (en) 2006-05-31 2007-12-06 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
WO2008150187A1 (fr) * 2007-06-08 2008-12-11 Jury Fedorovich Drygin Utilisation du trichloracétate d'ammonium en tant que produit de dissociation de complexes naturels d'acides nucléiques et procédé d'extraction d'arn
US20110287951A1 (en) * 2009-01-30 2011-11-24 Emmert-Buck Michael R Methods and systems for purifying, transferring, and/or manipulating nucleic acids
EP2414545B1 (de) 2009-04-03 2017-01-11 Sequenom, Inc. Zusammensetzungen und verfahren für die herstellung von nukleinsäuren
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
TWI407994B (zh) 2009-10-22 2013-09-11 Ind Tech Res Inst 分離核酸的方法、試劑及套組
WO2011080160A1 (en) 2009-12-29 2011-07-07 Whatman Inc Improvements to nucleic acid elution
HUE034870T2 (hu) 2010-01-07 2018-03-28 Bigtec Private Ltd Eljárás nukleinsavak izolációjára és ennek készlete
DE102010036111B4 (de) 2010-09-01 2013-08-29 Bundesrepublik Deutschland (Bundesamt für Ausrüstung, Informationstechnik und Nutzung der Bundeswehr) Vorbehandlungsverfahren zur Isolierung und Aufkonzentrierung von Nukleinsäuren aus einer Umweltprobe
WO2012075133A1 (en) 2010-11-30 2012-06-07 Life Technologies Corporation Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples
US11525155B2 (en) 2013-03-18 2022-12-13 Qiagen Gmbh Stabilisation of biological samples
WO2015082495A1 (en) * 2013-12-02 2015-06-11 Biocartis N.V. Extraction of circulating nucleic acids
DE102014211221A1 (de) * 2014-06-12 2015-12-17 Robert Bosch Gmbh Verfahren zur Anreicherung und/oder Aufreinigung von Nukleinsäuren
CN105734044A (zh) * 2014-12-08 2016-07-06 苏州新波生物技术有限公司 用于核酸提取纯化的漂洗液
TR201606310A2 (tr) 2016-05-12 2017-11-21 Akdeniz Ueniversitesi Genomik dna izolasyon yöntemi ve kiti.
CA3025569A1 (en) * 2016-05-27 2017-11-30 Norgen Biotek Corp. Preservation of cell-free nucleic acids in biological samples
CN110072989A (zh) * 2016-10-24 2019-07-30 生物马特里卡公司 核酸在纸上的稳定化
WO2018195594A1 (en) * 2017-04-24 2018-11-01 The University Of Queensland Simple nucleic acid extraction

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8507706D0 (en) 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
JPH02289598A (ja) 1989-04-28 1990-11-29 Yamanouchi Pharmaceut Co Ltd Ｍｓ―１１４４物質
DE69032383T2 (de) * 1989-08-04 1998-12-10 Behringwerke AG, 35041 Marburg Heterogene Bindungstests
JPH03101689A (ja) 1989-09-14 1991-04-26 Tosoh Corp 核酸の抽出方法又は除去方法
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US6103127A (en) * 1993-06-08 2000-08-15 Cortex Biochem, Inc. Methods for removing hazardous organic molecules from liquid waste
US5564104A (en) * 1993-06-08 1996-10-08 Cortex Biochem, Inc. Methods of removing radioactively labled biological molecules from liquid radioactive waste
JP3451667B2 (ja) 1993-08-24 2003-09-29 東ソー株式会社 核酸抽出及び特定核酸配列の検出方法
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
DE19530132C2 (de) * 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
AU2001238616A1 (en) 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
DE60137652D1 (de) * 2001-02-16 2009-03-26 Cortex Biochem Inc Magnetische isolierung und reinigung von nukleinsäuren
JP3979996B2 (ja) 2001-10-12 2007-09-19 ジェントラ システムズ インコーポレイテッド 固体支持体を使用してｒｎａを精製するための組成物および方法
WO2003040687A2 (en) * 2001-11-06 2003-05-15 Cortex Biochem, Inc. Isolation and purification of nucleic acids

Also Published As

Publication number Publication date
JP4277115B2 (ja) 2009-06-10
EP2000533A2 (de) 2008-12-10
AU2002333673B2 (en) 2008-08-14
EP2258845B1 (de) 2017-08-23
ATE491790T1 (de) 2011-01-15
EP1442045B1 (de) 2009-01-07
JP2009131264A (ja) 2009-06-18
WO2003040687A3 (en) 2003-12-18
CA2465961A1 (en) 2003-05-15
DE60238648D1 (de) 2011-01-27
ES2321492T3 (es) 2009-06-08
WO2003040687A2 (en) 2003-05-15
ATE420165T1 (de) 2009-01-15
JP5554919B2 (ja) 2014-07-23
EP1442045A4 (de) 2006-09-27
EP2258845A2 (de) 2010-12-08
EP2000533B1 (de) 2010-12-15
EP1442045A2 (de) 2004-08-04
EP2258845A3 (de) 2012-03-21
US20030092045A1 (en) 2003-05-15
DK1442045T3 (da) 2009-02-09
EP2000533A3 (de) 2009-07-08
JP2005508641A (ja) 2005-04-07
US7264927B2 (en) 2007-09-04

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: PROMEGA CORP., MADISON, WIS., US

8328 Change in the person/name/address of the agent

Representative=s name: STOFFREGEN, H., DIPL.-PHYS. DR.RER.NAT., PAT.-ANW.

8364 No opposition during term of opposition