DE60223299D1 - Verfahren zur Sequenzierung von Nukleinsäuren - Google Patents
Verfahren zur Sequenzierung von NukleinsäurenInfo
- Publication number
- DE60223299D1 DE60223299D1 DE60223299T DE60223299T DE60223299D1 DE 60223299 D1 DE60223299 D1 DE 60223299D1 DE 60223299 T DE60223299 T DE 60223299T DE 60223299 T DE60223299 T DE 60223299T DE 60223299 D1 DE60223299 D1 DE 60223299D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acids
- sequencing nucleic
- sequencing
- acids
- nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Nanotechnology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26297301P | 2001-01-20 | 2001-01-20 | |
| US10/052,926 US20020197618A1 (en) | 2001-01-20 | 2002-01-16 | Synthesis and amplification of unstructured nucleic acids for rapid sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60223299D1 true DE60223299D1 (de) | 2007-12-20 |
Family
ID=26731254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60223299T Expired - Lifetime DE60223299D1 (de) | 2001-01-20 | 2002-01-21 | Verfahren zur Sequenzierung von Nukleinsäuren |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20020197618A1 (de) |
| EP (1) | EP1225234B1 (de) |
| DE (1) | DE60223299D1 (de) |
Families Citing this family (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| WO2002072892A1 (en) | 2001-03-12 | 2002-09-19 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension |
| US7410564B2 (en) * | 2003-01-27 | 2008-08-12 | Agilent Technologies, Inc. | Apparatus and method for biopolymer identification during translocation through a nanopore |
| US7282130B2 (en) * | 2003-01-31 | 2007-10-16 | Agilent Technologies, Inc. | Apparatus and method for control of biopolymer translocation through a nanopore |
| WO2004080466A1 (en) * | 2003-03-07 | 2004-09-23 | Ribapharm Inc. | Cytidine analogs and methods of use |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| WO2005080605A2 (en) | 2004-02-19 | 2005-09-01 | Helicos Biosciences Corporation | Methods and kits for analyzing polynucleotide sequences |
| US7279337B2 (en) * | 2004-03-10 | 2007-10-09 | Agilent Technologies, Inc. | Method and apparatus for sequencing polymers through tunneling conductance variation detection |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| CA2566806A1 (en) | 2004-05-25 | 2006-01-19 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US20060228717A1 (en) * | 2005-04-08 | 2006-10-12 | Joyce Timothy H | Microfluidic system and method of utilization |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US20100291548A1 (en) | 2006-03-12 | 2010-11-18 | Applera Corporation | Methods of Detecting Target Nucleic Acids |
| DE102006035072B4 (de) * | 2006-07-28 | 2009-03-12 | Westfälische Wilhelms-Universität Münster | Vorrichtung und Verfahren zum Erfassen von Partikeln mit Pipette und Nanopore |
| GB0713143D0 (en) * | 2007-07-06 | 2007-08-15 | Ucl Business Plc | Nucleic acid detection method |
| CN102084001B (zh) | 2008-03-28 | 2015-03-18 | 加利福尼亚太平洋生物科学股份有限公司 | 用于核酸测序的组合物和方法 |
| US8628940B2 (en) | 2008-09-24 | 2014-01-14 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| WO2009120374A2 (en) | 2008-03-28 | 2009-10-01 | Pacific Biosciences Of California, Inc. | Methods and compositions for nucleic acid sample preparation |
| EP2682460B1 (de) | 2008-07-07 | 2017-04-26 | Oxford Nanopore Technologies Limited | Enzym-Pore Konstrukt |
| US8383369B2 (en) | 2008-09-24 | 2013-02-26 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| WO2010036287A1 (en) * | 2008-09-24 | 2010-04-01 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| WO2010037085A1 (en) | 2008-09-29 | 2010-04-01 | The Board Of Trustees Of The University Of Illinois | Dna sequencing and amplification systems using nanoscale field effect sensor arrays |
| CN102369298B (zh) | 2009-01-30 | 2017-03-22 | 牛津纳米孔技术有限公司 | 跨膜测序中用于核酸构建体的衔接体 |
| WO2010117470A2 (en) | 2009-04-10 | 2010-10-14 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| US9017937B1 (en) | 2009-04-10 | 2015-04-28 | Pacific Biosciences Of California, Inc. | Nanopore sequencing using ratiometric impedance |
| US9376713B2 (en) | 2009-09-23 | 2016-06-28 | The Board Of Trustees Of The University Of Illinois | Label free detection of nucleic acid amplification |
| US8652779B2 (en) | 2010-04-09 | 2014-02-18 | Pacific Biosciences Of California, Inc. | Nanopore sequencing using charge blockade labels |
| US20120034603A1 (en) * | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| IN2014DN00221A (de) | 2011-07-25 | 2015-06-05 | Oxford Nanopore Tech Ltd | |
| EP2758545B1 (de) | 2011-09-23 | 2017-07-26 | Oxford Nanopore Technologies Limited | Analyse eines polymers mit polymereinheiten |
| WO2013119784A1 (en) * | 2012-02-08 | 2013-08-15 | Brown University | Methods of sequencing nucleic acids using nanopores and active kinetic proofreading |
| CA2864035C (en) | 2012-02-15 | 2021-05-18 | Oxford Nanopore Technologies Limited | A method for determining the presence of an analyte using an aptamer |
| US9353393B2 (en) * | 2012-02-15 | 2016-05-31 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| CA2866587C (en) | 2012-02-16 | 2022-07-26 | Oxford Nanopore Technologies Limited | Analysis of measurements of a polymer |
| US9116118B2 (en) * | 2012-06-08 | 2015-08-25 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| EP2875154B1 (de) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB-Verfahren zur Charakterisierung einer Nukleinsäure |
| WO2014041337A1 (en) * | 2012-09-14 | 2014-03-20 | Oxford Nanopore Technologies Limited | Sample preparation method |
| US9995728B2 (en) | 2012-11-06 | 2018-06-12 | Oxford Nanopore Technologies Ltd. | Quadruplex method |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| KR102168813B1 (ko) | 2013-03-08 | 2020-10-22 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 효소 정지 방법 |
| US10184930B2 (en) * | 2013-11-26 | 2019-01-22 | Stc.Unm | Innovative nanopore sequencing technology |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| EP3971300B1 (de) | 2014-10-16 | 2024-06-05 | Oxford Nanopore Technologies plc | Sortierung von polymeren |
| CN113981055A (zh) | 2014-10-17 | 2022-01-28 | 牛津纳米孔技术公司 | 纳米孔rna表征方法 |
| US10626455B2 (en) | 2014-11-11 | 2020-04-21 | Bgi Shenzhen | Multi-pass sequencing |
| US11098348B2 (en) * | 2015-02-02 | 2021-08-24 | Ontera Inc. | Nanopore detection of target polynucleotides from sample background |
| US10208339B2 (en) * | 2015-02-19 | 2019-02-19 | Takara Bio Usa, Inc. | Systems and methods for whole genome amplification |
| CN104897728B (zh) * | 2015-06-01 | 2017-10-03 | 中国科学院重庆绿色智能技术研究院 | 基于微纳米孔网集成结构的纳米孔检测系统及其制备方法 |
| WO2017003924A1 (en) * | 2015-06-29 | 2017-01-05 | Genesis DNA Inc. | Method and apparatus for dual solid phase nucleic acid synthesis |
| CA2995422A1 (en) | 2015-08-12 | 2017-02-16 | The Chinese University Of Hong Kong | Single-molecule sequencing of plasma dna |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| EP3535404A2 (de) * | 2016-11-07 | 2019-09-11 | Ibis Biosciences, Inc. | Modifizierte nukleinsäuren zur nanoporenanalyse |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| WO2020113581A1 (zh) * | 2018-12-07 | 2020-06-11 | 深圳华大生命科学研究院 | 纳米孔测序方法 |
| DE102019200929A1 (de) * | 2019-01-25 | 2020-07-30 | Robert Bosch Gmbh | Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls |
| WO2022165276A1 (en) | 2021-01-29 | 2022-08-04 | Armonica Technologies, Inc. | Enhancement structures for surface-enhanced raman scattering |
| CN117594130B (zh) * | 2024-01-19 | 2024-07-16 | 北京普译生物科技有限公司 | 纳米孔测序信号评价方法、装置、电子设备和存储介质 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5714320A (en) * | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
| US5871918A (en) * | 1996-06-20 | 1999-02-16 | The University Of North Carolina At Chapel Hill | Electrochemical detection of nucleic acid hybridization |
| US5795782A (en) * | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5912340A (en) * | 1995-10-04 | 1999-06-15 | Epoch Pharmaceuticals, Inc. | Selective binding complementary oligonucleotides |
| WO1997019193A2 (en) * | 1995-11-21 | 1997-05-29 | Yale University | Unimolecular segment amplification and detection |
| GB9609213D0 (en) * | 1996-05-02 | 1996-07-03 | Tepnel Medical Ltd | Amplification of nucleic acids |
| US6054274A (en) * | 1997-11-12 | 2000-04-25 | Hewlett-Packard Company | Method of amplifying the signal of target nucleic acid sequence analyte |
| US6175004B1 (en) * | 1998-09-01 | 2001-01-16 | Isis Pharmaceuticals, Inc. | Process for the synthesis of oligonucleotides incorporating 2-aminoadenosine |
| US20040086880A1 (en) * | 1999-07-20 | 2004-05-06 | Sampson Jeffrey R | Method of producing nucleic acid molecules with reduced secondary structure |
| US20030104376A1 (en) * | 2000-05-10 | 2003-06-05 | Veronica Brockhurst | Assay |
| US6693187B1 (en) * | 2000-10-17 | 2004-02-17 | Lievre Cornu Llc | Phosphinoamidite carboxlates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge |
-
2002
- 2002-01-16 US US10/052,926 patent/US20020197618A1/en not_active Abandoned
- 2002-01-21 EP EP02250379A patent/EP1225234B1/de not_active Expired - Lifetime
- 2002-01-21 DE DE60223299T patent/DE60223299D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US20020197618A1 (en) | 2002-12-26 |
| EP1225234A2 (de) | 2002-07-24 |
| EP1225234B1 (de) | 2007-11-07 |
| EP1225234A3 (de) | 2004-07-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8332 | No legal effect for de |