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DE333253T1 - Einrichtung und geraet fuer die detektion von mikroorganismen. - Google Patents

Einrichtung und geraet fuer die detektion von mikroorganismen.

Info

Publication number
DE333253T1
DE333253T1 DE198989200554T DE89200554T DE333253T1 DE 333253 T1 DE333253 T1 DE 333253T1 DE 198989200554 T DE198989200554 T DE 198989200554T DE 89200554 T DE89200554 T DE 89200554T DE 333253 T1 DE333253 T1 DE 333253T1
Authority
DE
Germany
Prior art keywords
container
sample
medium
indicator
sensing means
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198989200554T
Other languages
English (en)
Inventor
Michael John Somerset Nj 08873 Calandra
Jr. James Lawrence Durham Nc 27712 Diguiseppi
Richard Cornelius Raleigh Nc 27614 Driscoll
Thurman Clay Durham Nc 27704 Thorpe
Jr. James Edward Chapel Hill Nc 27514 Turner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akzo NV
Original Assignee
Akzo NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo NV filed Critical Akzo NV
Publication of DE333253T1 publication Critical patent/DE333253T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (10)

AKZO N.V. 32251 Ansprüche
1. Vorrichtung zum Nachweis von Mikroorganismen in einer Probe, umfassend einen verschließbaren und sterilisierbaren Probenbehälter mit einer Innenkammer, in der eine Probe mit einem sterilen Kulturmedium zum Nachweis von mikrobieller Verunreinigung in der Probe gezüchtet werden kann, und mindestens einem transparenten Abschnitt in der Wand jenes Behälters, sowie ein an der Innenfläche der Wand jenes Behälters im Bereich des transparenten Abschnittes angebrachtes Fühlermittel, wodurch Veränderungen im Aussehen des Fühlermittels von der Außenseite jenes Behälters her durch jenen transparenten Abschnitt erkennbar sind, wobei jenes Fühlermittel ein immobilisiertes Indikatormedium umfaßt, das wegen seiner Fähigkeit zur Anzeige einer erkennbaren Veränderung bei Exposition mit Produkten der Stoffwechseltätigkeit eines Organismus ausgewählt wurde, wobei jenes Indikatormedium nach dem Verfahren der Bindung des Indikators au ein Trägermedium oder Verkapselung des Indikators in einer Polymermatrix immobilisiert wird.
2. Vorrichtung nach Anspruch 1, worin das Indikatormedium eine pH-abhängige Molekülart ist.
3. Vorrichtung nach Anspruch 1, worin das ( Fühlermittel eine aufgeladene Membran umfaßt. j
4. Vorrichtung nach Anspruch 1/ worin die Membran des Fühlermittels mit der Innenfläche jenes Behälters verbunden ist.
5. Vorrichtung nach Anspruch 4, worin die Membran des Fühlermittels wenigstens am Umfang mit der
Innenfläche des Behälters verbunden ist, wodurch weder die Probe noch das Kulturmedium zwischen der Membran und der Behälteroberfläche hindurchlaugen können.
6. Vorrichtung nach Anspruch 1, worin das Fühlermittel von der Probe in jenem Behälter durch eine semipermeable Membran getrennt ist.
7. Vorrichtung nach Anspruch 6, worin die semipermeable Membran aus einer in situ gebildeten Membran besteht..
8. Vorrichtung nach Anspruch 1, worin das Fühlermittel Mizellen von in einer Polymermatrix immobilisiertem Indikatormedium darstellt.
9. Vorrichtung nach Anspruch 8, worin die Mizellen aus Indikatormedium in der Polymermatrix nach dem Verfahren der Emulgierung einer Indikatorlösung in einem ungehärteten Polymer und Aushärtung des Polymeren gebildet wurden.
10. Methode zum Nachweis von Mikroorganismen in einer Probe, umfassend die Schritte der Einführung der Probe in einen sterilen Probenbehälter unter sterilen Bedingungen, wobei der Probenbehälter mindestens einen transparenten Abschnitt in seiner Wand und ein Fühlermittel, das ein bei Exposition mit gasförmigen Produkten der Stoffwechselaktivität eines Organismus eine erkennbare Veränderung anzeigendes und an der Innenfläche der Wand des Behälters im Bereich des transparenten Abschnitts angebrachtes Indikatormedium umfaßt, der Inkubation der Probe im Probenbehälter mit einem Mikrobenwachstumsmedium, wobei der Probenbehälter in einer solchen Position orientiert ist, daß das Fühlermittel völlig in die Probe und das Wachstumsmedium eingetaucht ist, und Verfolgung der Produktion von gasförmigen Produkten der Stoffwechselaktivität eines Organismus von der Außenseite des Behälters her durch seinen transparenten Abschnitt ohne Verletzung der Behälterintegrität durch Erkennen von Veränderungen, die im Indikatormedium im Fühlermittel angezeigt werden und von den von der Probe
03332ö3
und dem Wachstumsmedium zum Fühlermittel gelangenden gasförmigen Produkten stammen.
DE198989200554T 1988-03-15 1989-03-06 Einrichtung und geraet fuer die detektion von mikroorganismen. Pending DE333253T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/168,291 US4945060A (en) 1988-03-15 1988-03-15 Device for detecting microorganisms

Publications (1)

Publication Number Publication Date
DE333253T1 true DE333253T1 (de) 1993-02-25

Family

ID=22610896

Family Applications (2)

Application Number Title Priority Date Filing Date
DE68923720T Expired - Lifetime DE68923720T2 (de) 1988-03-15 1989-03-06 Einrichtung und Gerät für die Detektion von Mikroorganismen.
DE198989200554T Pending DE333253T1 (de) 1988-03-15 1989-03-06 Einrichtung und geraet fuer die detektion von mikroorganismen.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE68923720T Expired - Lifetime DE68923720T2 (de) 1988-03-15 1989-03-06 Einrichtung und Gerät für die Detektion von Mikroorganismen.

Country Status (13)

Country Link
US (1) US4945060A (de)
EP (1) EP0333253B1 (de)
JP (1) JP2862556B2 (de)
KR (1) KR970003150B1 (de)
AT (1) ATE126267T1 (de)
AU (1) AU631533B2 (de)
CA (1) CA1339512C (de)
DE (2) DE68923720T2 (de)
DK (1) DK123889A (de)
ES (1) ES2031807T3 (de)
GR (1) GR3017974T3 (de)
IE (1) IE67634B1 (de)
ZA (1) ZA891788B (de)

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SE439927B (sv) * 1984-02-10 1985-07-08 Sangtec Medical Ab Apparat och forfarande for registrering av bakterieforekomst, speciellt under feltmessiga forhallanden
JPS61186854A (ja) * 1985-02-14 1986-08-20 Fuji Photo Film Co Ltd 超純水中のバクテリア数測定装置
DD253570A1 (de) * 1986-09-09 1988-01-27 Medizin Labortechnik Veb K Ueberzug fuer schlauchsysteme
US4780191A (en) * 1987-06-26 1988-10-25 Massachusetts Institute Of Technology L-glutamine sensor
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DK123889A (da) 1989-09-16
IE67634B1 (en) 1996-04-17
US4945060A (en) 1990-07-31
ES2031807T1 (es) 1993-01-01
IE890746L (en) 1989-09-15
ZA891788B (en) 1990-03-28
ES2031807T3 (es) 1995-12-16
KR970003150B1 (ko) 1997-03-14
AU3128889A (en) 1989-09-21
DE68923720D1 (de) 1995-09-14
EP0333253A2 (de) 1989-09-20
GR3017974T3 (en) 1996-02-29
AU631533B2 (en) 1992-12-03
JP2862556B2 (ja) 1999-03-03
JPH0216965A (ja) 1990-01-19
KR890014749A (ko) 1989-10-25
DK123889D0 (da) 1989-03-14
EP0333253A3 (en) 1990-01-17
DE68923720T2 (de) 1996-02-08
EP0333253B1 (de) 1995-08-09
CA1339512C (en) 1997-10-28
ATE126267T1 (de) 1995-08-15

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