[go: up one dir, main page]

CY1109285T1 - Ανακτηση toy dna - Google Patents

Ανακτηση toy dna

Info

Publication number
CY1109285T1
CY1109285T1 CY20091100852T CY091100852T CY1109285T1 CY 1109285 T1 CY1109285 T1 CY 1109285T1 CY 20091100852 T CY20091100852 T CY 20091100852T CY 091100852 T CY091100852 T CY 091100852T CY 1109285 T1 CY1109285 T1 CY 1109285T1
Authority
CY
Cyprus
Prior art keywords
mrna
cdna
methods
recovery
region
Prior art date
Application number
CY20091100852T
Other languages
English (en)
Inventor
Mingyue He
Alison M Jackson
Original Assignee
Crescendo Biologics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0306305A external-priority patent/GB0306305D0/en
Priority claimed from GB0311351A external-priority patent/GB0311351D0/en
Application filed by Crescendo Biologics Ltd filed Critical Crescendo Biologics Ltd
Publication of CY1109285T1 publication Critical patent/CY1109285T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Control Of Throttle Valves Provided In The Intake System Or In The Exhaust System (AREA)
  • Valve Device For Special Equipments (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Η εφεύρεση προσφέρει κάποια μέθοδο για την ανάκτηση cDNA από mRNA, η οποία περιλαμβάνει αντίστροφη μεταγραφή (RT) του mRNA χρησιμοποιώντας κάποιον εκκινητή RT που περιλαμβάνει κάποια αλληλουχία βασισμένη στην 5' συναινετική περιοχή του mRNA η οποία είναι πανομοιότυπη ή παρόμοια με την 5' συναινετική περιοχή του mRNA και η οποία περιλαμβάνει κάποια αλληλουχία ικανή να υβριδοποιείται ειδικά με την 3' περιοχή του mRNA, ακολουθούμενη από αλυσιδωτή αντίδραση πολυμεράσης (PCR) χρησιμοποιώντας ένα μόνο εκκινητή για την δημιουργία ss cDNA, ds cDNA και την ενίσχυση του cDNA. Παρέχονται επίσης οι εκκινητές που χρησιμοποιούνται στις μεθόδους της εφεύρεσης και τα κιτ για την πραγματοποίηση των μεθόδων της εφεύρεσης. Οι μέθοδοι της εφεύρεσης μπορεί να είναι πλήρως ή μερικώς αυτοματοποιημένες.
CY20091100852T 2003-03-19 2009-08-11 Ανακτηση toy dna CY1109285T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0306305A GB0306305D0 (en) 2003-03-19 2003-03-19 Recovery of DNA
GB0311351A GB0311351D0 (en) 2003-05-16 2003-05-16 Activity-directed in vitro protein cloning and identification
EP04721937A EP1604044B1 (en) 2003-03-19 2004-03-19 Recovery of dna

Publications (1)

Publication Number Publication Date
CY1109285T1 true CY1109285T1 (el) 2014-07-02

Family

ID=33031411

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091100852T CY1109285T1 (el) 2003-03-19 2009-08-11 Ανακτηση toy dna

Country Status (9)

Country Link
US (1) US7572577B2 (el)
EP (1) EP1604044B1 (el)
AT (1) ATE431430T1 (el)
CY (1) CY1109285T1 (el)
DE (1) DE602004021094D1 (el)
DK (1) DK1604044T3 (el)
ES (1) ES2326649T3 (el)
PT (1) PT1604044E (el)
WO (2) WO2004083428A1 (el)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3629022A1 (en) 2008-07-25 2020-04-01 Richard W. Wagner Protein screening methods
WO2018005283A1 (en) * 2016-06-27 2018-01-04 Dana-Farber Cancer Institute, Inc. Methods for measuring rna translation rates
CN110904135B (zh) * 2019-12-30 2021-08-13 苏州珀罗汀生物技术有限公司 蛋白质基础表达体系、合成系统及制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
CA2080305A1 (en) 1991-10-11 1993-04-12 Linda M. Western Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
WO1998054312A1 (en) * 1997-05-28 1998-12-03 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
US6207424B1 (en) * 1999-11-23 2001-03-27 Maxim Biotech, Inc. Self-primed amplification system
US6379932B1 (en) 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA

Also Published As

Publication number Publication date
WO2004083461A9 (en) 2004-12-29
WO2004083428A1 (en) 2004-09-30
US7572577B2 (en) 2009-08-11
PT1604044E (pt) 2009-08-05
ES2326649T3 (es) 2009-10-16
DK1604044T3 (da) 2009-08-31
EP1604044A1 (en) 2005-12-14
DE602004021094D1 (de) 2009-06-25
ATE431430T1 (de) 2009-05-15
EP1604044B1 (en) 2009-05-13
US20060281086A1 (en) 2006-12-14
WO2004083461A1 (en) 2004-09-30

Similar Documents

Publication Publication Date Title
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
WO2003062389A3 (en) UNIVERSAL RT-COUPLED PCR METHOD FOR THE SPECIFIC AMPLIFICATION OF mRNA
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
DE112006002611A5 (de) Rna-abhängige rna-polymerase, verfahren und kits zur amplifikation und / oder markierung von rna
EP1845160A4 (en) NUCLEOTIDE-amplification
DE602004030156D1 (de) Verfahren zur quantitativen bewertung einer umordnung oder einer gezielten genetischen rekombination bei einem individuum sowie verwendungen davon
WO2002044399A3 (en) In vitro transcription method for rna amplification
BR0101493B1 (pt) mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes.
NO20021223L (no) Fremgangsmater og sammensetninger for lineaer, isoterm amplifisering av polynukleotidsekvenser
WO2005091971A3 (en) Compositions for use in identification of alphaviruses
DE60038796D1 (de) Hochspezifische primer, amplifizierungsverfahren und kits
WO2008033442A3 (en) Methods and compositions for performing low background multiplex nucleic acid amplification reactions
ATE437238T1 (de) Vermehrung von ribonukleinsäuren
ATE476523T1 (de) Verfahren zur genomamplifikation
WO2004068112A3 (en) Hairpin primer amplification
WO2002101004A3 (en) Low-temperature cycle extension of dna with high priming specificity
CY1109285T1 (el) Ανακτηση toy dna
ATE539167T1 (de) Verfahren zur amplifikation von rna-sequenzen
DE60331072D1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
WO2006081463A3 (en) Thermococcus zilligii dna polymerases and variants thereof
DE502006008330D1 (de) Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
WO2008045431A3 (en) Rapid reverse transcription of rna
ATE448326T1 (de) Verfahren zur amplifikation genetischer information mittels an mehrere stellen im genom bindende primer
ATE470710T1 (de) Auf nasba basiertes detektionsverfahren zur detektion des tuberkelbazillus
WO2005070027A8 (en) A novel nucleotide mixture for improved nucleic acid amplification performance