ATE431430T1 - Gewinnung von dna - Google Patents
Gewinnung von dnaInfo
- Publication number
- ATE431430T1 ATE431430T1 AT04721937T AT04721937T ATE431430T1 AT E431430 T1 ATE431430 T1 AT E431430T1 AT 04721937 T AT04721937 T AT 04721937T AT 04721937 T AT04721937 T AT 04721937T AT E431430 T1 ATE431430 T1 AT E431430T1
- Authority
- AT
- Austria
- Prior art keywords
- mrna
- cdna
- methods
- region
- obtaining dna
- Prior art date
Links
- 108020004999 messenger RNA Proteins 0.000 abstract 5
- 239000002299 complementary DNA Substances 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 4
- 238000010839 reverse transcription Methods 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 238000011084 recovery Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Control Of Throttle Valves Provided In The Intake System Or In The Exhaust System (AREA)
- Valve Device For Special Equipments (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0306305A GB0306305D0 (en) | 2003-03-19 | 2003-03-19 | Recovery of DNA |
GB0311351A GB0311351D0 (en) | 2003-05-16 | 2003-05-16 | Activity-directed in vitro protein cloning and identification |
PCT/GB2004/001211 WO2004083461A1 (en) | 2003-03-19 | 2004-03-19 | Recovery of dna |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE431430T1 true ATE431430T1 (de) | 2009-05-15 |
Family
ID=33031411
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT04721937T ATE431430T1 (de) | 2003-03-19 | 2004-03-19 | Gewinnung von dna |
Country Status (9)
Country | Link |
---|---|
US (1) | US7572577B2 (de) |
EP (1) | EP1604044B1 (de) |
AT (1) | ATE431430T1 (de) |
CY (1) | CY1109285T1 (de) |
DE (1) | DE602004021094D1 (de) |
DK (1) | DK1604044T3 (de) |
ES (1) | ES2326649T3 (de) |
PT (1) | PT1604044E (de) |
WO (2) | WO2004083461A1 (de) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20110036638A (ko) | 2008-07-25 | 2011-04-07 | 리차드 더블유. 와그너 | 단백질 스크리닝 방법 |
WO2018005283A1 (en) * | 2016-06-27 | 2018-01-04 | Dana-Farber Cancer Institute, Inc. | Methods for measuring rna translation rates |
CN110904135B (zh) * | 2019-12-30 | 2021-08-13 | 苏州珀罗汀生物技术有限公司 | 蛋白质基础表达体系、合成系统及制备方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
ES2112302T3 (es) * | 1991-10-11 | 1998-04-01 | Behringwerke Ag | Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos. |
JP4060892B2 (ja) * | 1997-05-28 | 2008-03-12 | ディサーナ リミテッド | タンパク質のin vitroディスプレイ及び進化のための選択粒子としてのリボソーム複合体 |
US6207424B1 (en) | 1999-11-23 | 2001-03-27 | Maxim Biotech, Inc. | Self-primed amplification system |
US6379932B1 (en) * | 2000-07-17 | 2002-04-30 | Incyte Genomics, Inc. | Single primer PCR amplification of RNA |
-
2004
- 2004-03-19 AT AT04721937T patent/ATE431430T1/de active
- 2004-03-19 DK DK04721937T patent/DK1604044T3/da active
- 2004-03-19 EP EP04721937A patent/EP1604044B1/de not_active Expired - Lifetime
- 2004-03-19 DE DE602004021094T patent/DE602004021094D1/de not_active Expired - Lifetime
- 2004-03-19 PT PT04721937T patent/PT1604044E/pt unknown
- 2004-03-19 WO PCT/GB2004/001211 patent/WO2004083461A1/en active Application Filing
- 2004-03-19 ES ES04721937T patent/ES2326649T3/es not_active Expired - Lifetime
- 2004-03-19 WO PCT/GB2004/001206 patent/WO2004083428A1/en active Application Filing
- 2004-03-19 US US10/549,778 patent/US7572577B2/en not_active Expired - Fee Related
-
2009
- 2009-08-11 CY CY20091100852T patent/CY1109285T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
DE602004021094D1 (de) | 2009-06-25 |
PT1604044E (pt) | 2009-08-05 |
WO2004083461A9 (en) | 2004-12-29 |
US20060281086A1 (en) | 2006-12-14 |
WO2004083428A1 (en) | 2004-09-30 |
CY1109285T1 (el) | 2014-07-02 |
EP1604044B1 (de) | 2009-05-13 |
EP1604044A1 (de) | 2005-12-14 |
WO2004083461A1 (en) | 2004-09-30 |
DK1604044T3 (da) | 2009-08-31 |
US7572577B2 (en) | 2009-08-11 |
ES2326649T3 (es) | 2009-10-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2004092418A3 (en) | Global amplification using a randomly primed composite primer | |
ATE432365T1 (de) | Verfahren, kits und reagenzien zur nukleinsäuresequenzierung | |
WO2003062389A3 (en) | UNIVERSAL RT-COUPLED PCR METHOD FOR THE SPECIFIC AMPLIFICATION OF mRNA | |
DE112006002611A5 (de) | Rna-abhängige rna-polymerase, verfahren und kits zur amplifikation und / oder markierung von rna | |
EP1845160A4 (de) | Nukleotidsequenz-amplifikationsverfahren | |
DE602004030156D1 (de) | Verfahren zur quantitativen bewertung einer umordnung oder einer gezielten genetischen rekombination bei einem individuum sowie verwendungen davon | |
BR0101493B1 (pt) | mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes. | |
SE0202867D0 (sv) | New sequencing method | |
ATE437238T1 (de) | Vermehrung von ribonukleinsäuren | |
ATE476523T1 (de) | Verfahren zur genomamplifikation | |
DE502004004202D1 (de) | Verfahren zum nachweis von cytosin-methylierungen in dna | |
EP1772523A3 (de) | Polymerase-Kettenreaktionsverfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaften | |
MX2021014430A (es) | Método para amplificar arnms y para preparar bibliotecas de arnm de longitud total. | |
CY1109285T1 (el) | Ανακτηση toy dna | |
JP5200302B2 (ja) | ヘリカーゼを使用するrnaターゲットの同定 | |
ATE539167T1 (de) | Verfahren zur amplifikation von rna-sequenzen | |
ATE455866T1 (de) | Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität | |
DE502006008330D1 (de) | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna | |
ATE448326T1 (de) | Verfahren zur amplifikation genetischer information mittels an mehrere stellen im genom bindende primer | |
WO2008045431A3 (en) | Rapid reverse transcription of rna | |
ATE470710T1 (de) | Auf nasba basiertes detektionsverfahren zur detektion des tuberkelbazillus | |
WO2006138716A3 (en) | Thermodynamically defined gene specific primers | |
ATE456669T1 (de) | Verfahren zur quantifizierung von nukleinsäuren | |
DE602005023586D1 (de) | Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'-nichttranslatierten bereichs von hcv | |
DE60318315D1 (de) | Nukleinsaure vervielfaeltigungsverfahren |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification |
Ref document number: 1604044 Country of ref document: EP |