[go: up one dir, main page]

DE60038796D1 - Hochspezifische primer, amplifizierungsverfahren und kits - Google Patents

Hochspezifische primer, amplifizierungsverfahren und kits

Info

Publication number
DE60038796D1
DE60038796D1 DE60038796T DE60038796T DE60038796D1 DE 60038796 D1 DE60038796 D1 DE 60038796D1 DE 60038796 T DE60038796 T DE 60038796T DE 60038796 T DE60038796 T DE 60038796T DE 60038796 D1 DE60038796 D1 DE 60038796D1
Authority
DE
Germany
Prior art keywords
primers
kits
specific primers
highly specific
amplification processes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60038796T
Other languages
English (en)
Inventor
Sanjay Tyagi
Fred R Kramer
Robert Vartikian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Rutgers Health
Original Assignee
University of Medicine and Dentistry of New Jersey
Rutgers State University of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Medicine and Dentistry of New Jersey, Rutgers State University of New Jersey filed Critical University of Medicine and Dentistry of New Jersey
Application granted granted Critical
Publication of DE60038796D1 publication Critical patent/DE60038796D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Dental Preparations (AREA)
  • Paints Or Removers (AREA)
DE60038796T 1999-05-24 2000-05-03 Hochspezifische primer, amplifizierungsverfahren und kits Expired - Lifetime DE60038796D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/317,350 US6277607B1 (en) 1999-05-24 1999-05-24 High specificity primers, amplification methods and kits
PCT/US2000/011979 WO2000071562A1 (en) 1999-05-24 2000-05-03 High specificity primers, amplification methods and kits

Publications (1)

Publication Number Publication Date
DE60038796D1 true DE60038796D1 (de) 2008-06-19

Family

ID=23233265

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60038796T Expired - Lifetime DE60038796D1 (de) 1999-05-24 2000-05-03 Hochspezifische primer, amplifizierungsverfahren und kits

Country Status (8)

Country Link
US (2) US6277607B1 (de)
EP (1) EP1185546B1 (de)
JP (1) JP3843215B2 (de)
AT (1) ATE394410T1 (de)
AU (1) AU769219B2 (de)
CA (1) CA2375027C (de)
DE (1) DE60038796D1 (de)
WO (1) WO2000071562A1 (de)

Families Citing this family (136)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6893868B2 (en) 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US7312302B2 (en) 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
TW593731B (en) * 1998-03-20 2004-06-21 Semitool Inc Apparatus for applying a metal structure to a workpiece
US6565729B2 (en) 1998-03-20 2003-05-20 Semitool, Inc. Method for electrochemically depositing metal on a semiconductor workpiece
US6497801B1 (en) * 1998-07-10 2002-12-24 Semitool Inc Electroplating apparatus with segmented anode array
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US7189318B2 (en) * 1999-04-13 2007-03-13 Semitool, Inc. Tuning electrodes used in a reactor for electrochemically processing a microelectronic workpiece
CN1296524C (zh) 1999-04-13 2007-01-24 塞米用具公司 对工件进行电化学处理的处理容器、反应器和方法
US7351315B2 (en) 2003-12-05 2008-04-01 Semitool, Inc. Chambers, systems, and methods for electrochemically processing microfeature workpieces
US7351314B2 (en) 2003-12-05 2008-04-01 Semitool, Inc. Chambers, systems, and methods for electrochemically processing microfeature workpieces
US6916412B2 (en) 1999-04-13 2005-07-12 Semitool, Inc. Adaptable electrochemical processing chamber
US7160421B2 (en) * 1999-04-13 2007-01-09 Semitool, Inc. Turning electrodes used in a reactor for electrochemically processing a microelectronic workpiece
US6368475B1 (en) * 2000-03-21 2002-04-09 Semitool, Inc. Apparatus for electrochemically processing a microelectronic workpiece
US7264698B2 (en) 1999-04-13 2007-09-04 Semitool, Inc. Apparatus and methods for electrochemical processing of microelectronic workpieces
US7438788B2 (en) 1999-04-13 2008-10-21 Semitool, Inc. Apparatus and methods for electrochemical processing of microelectronic workpieces
US7020537B2 (en) 1999-04-13 2006-03-28 Semitool, Inc. Tuning electrodes used in a reactor for electrochemically processing a microelectronic workpiece
US7585398B2 (en) 1999-04-13 2009-09-08 Semitool, Inc. Chambers, systems, and methods for electrochemically processing microfeature workpieces
NZ516278A (en) 1999-06-22 2004-03-26 Invitrogen Corp Improved primers and methods for the detection and discrimination of nucleic acids
US6692915B1 (en) * 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
ATE317023T1 (de) * 1999-11-08 2006-02-15 Eiken Chemical Methode zum nachweis von variationen oder polymorphismen
HK1052203A1 (zh) * 1999-11-08 2003-09-05 荣研化学株式会社 檢測突變和/或多態性的方法
US6664081B2 (en) * 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
US7205129B1 (en) 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
DK1188841T3 (da) * 2000-03-31 2009-04-20 Sanko Junyaku Kk Probe til fremstilling af en polymerprobe, fremgangsmåde til fremstilling af en polymerprobe samt anvendelse af polymerproben
ES2464132T3 (es) * 2000-04-07 2014-05-30 Eiken Kagaku Kabushiki Kaisha Procedimiento de amplificación de ácido nucleico utilizando como molde ácido nucleico bicatenario
US7659054B1 (en) * 2000-05-23 2010-02-09 Nuvelo, Inc. Methods for genetic analysis of DNA to detect sequence variances
US6531283B1 (en) 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6380377B1 (en) 2000-07-14 2002-04-30 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP1337541B1 (de) 2000-10-06 2007-03-07 The Trustees of Columbia University in the City of New York Massives Parallelverfahren zur Dekodierung von DNA und RNA
CN1348096A (zh) * 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
US6861222B2 (en) * 2000-11-09 2005-03-01 Yale University Nucleic acid detection using structured probes
AU2002230734A1 (en) 2000-12-11 2002-06-24 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification
GB0101397D0 (en) * 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US20020102557A1 (en) * 2001-01-31 2002-08-01 Gentile-Davey Maria C. Self -complementary molecular probe
US20020142336A1 (en) * 2001-02-02 2002-10-03 Genome Therapeutics Corporation Methods for determining a nucleotide at a specific location within a nucleic acid molecule
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
JP2004532026A (ja) * 2001-03-30 2004-10-21 アメルシャム・バイオサイエンシーズ・アクチボラグ Ｐ４５０単一ヌクレオチド多型バイオチップ分析
US7067849B2 (en) * 2001-07-17 2006-06-27 Lg Electronics Inc. Diode having high brightness and method thereof
US7090751B2 (en) 2001-08-31 2006-08-15 Semitool, Inc. Apparatus and methods for electrochemical processing of microelectronic workpieces
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US7393636B2 (en) * 2001-11-08 2008-07-01 Sanko Junyaku Co., Ltd. Method for forming self-assembly substance using oligonucleotide synthesized by gene amplification reaction, self-assembly substance and method for detecting gene
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US7291459B2 (en) * 2002-12-10 2007-11-06 University Of Alabama At Huntsville Nucleic acid detector and method of detecting targets within a sample
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
AU2003299867A1 (en) * 2002-12-27 2004-07-29 University Of Medicine And Dentistry Of New Jersey Method for single nucleotide polymorphism detection
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
WO2004061127A2 (en) * 2003-01-02 2004-07-22 University Of Rochester Hybridization-based biosensor containing hairpin probes and use thereof
US20040259116A1 (en) * 2003-01-28 2004-12-23 Gorilla Genomics, Inc. Hairpin primer amplification
JP4608483B2 (ja) * 2003-02-18 2011-01-12 アプライド バイオシステムズ， エルエルシー ポリヌクレオチドの複合的分析のための組成物および方法
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
AU2003229256A1 (en) * 2003-05-09 2004-11-26 Capital Biochip Company, Ltd. Methods and compositions for optimizing multiplex pcr primers
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
DE10338308B4 (de) * 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
CA2551842A1 (en) * 2004-01-02 2005-11-10 University Of Rochester Method of identifying hairpin dna probes by partial fold analysis
US7361469B2 (en) * 2004-08-13 2008-04-22 Stratagene California Dual labeled fluorescent probes
CN101076607B (zh) 2004-10-18 2013-04-24 布兰迪斯大学 用于核酸扩增的引物、探针和方法
US20060194222A1 (en) * 2004-10-20 2006-08-31 Stratagene California Triplex probe compositions and methods for polynucleotide detection
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US20070213293A1 (en) * 2005-04-08 2007-09-13 Nastech Pharmaceutical Company Inc. Rnai therapeutic for respiratory virus infection
EP1874802A2 (de) * 2005-04-08 2008-01-09 Nastech Pharmaceutical Company Inc. Rnai-therapie für virale atemwegsinfektion
WO2006113770A1 (en) 2005-04-15 2006-10-26 Epigenomics Ag A method for providing dna fragments derived from a remote sample
JP2008539730A (ja) * 2005-05-02 2008-11-20 ストラタジーン カリフォルニア オリゴヌクレオチドのプローブ／プライマー組成物、及びポリヌクレオチドの検出方法
EP1910397A4 (de) * 2005-07-15 2009-07-01 Applera Corp Entwurf einer heissstart-rückübertragung durch primer
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
WO2007046158A1 (ja) * 2005-10-20 2007-04-26 Hitachi, Ltd. 核酸分析方法
EP1951905B1 (de) 2005-11-10 2012-04-11 Affymetrix, Inc. Nachweis von nukleinsäuren über amplifikation von stellvertreternukleinsäuren
US20070243534A1 (en) * 2006-04-12 2007-10-18 Michael Seul Probe density considerations and elongation of self-complementary looped probes where probes are attached to a solid phase
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
DK2017356T3 (da) * 2006-06-06 2012-01-30 Gen Probe Inc Mærkede oligonukleotider og anvendelse deraf i fremgangsmåder til amplifikation af nukleinsyrer
AU2007275762B2 (en) 2006-07-17 2012-02-23 Brandeis University Specialized oligonucleotides and their use in nucleic acid amplification and detection
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8198027B2 (en) * 2006-12-21 2012-06-12 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP2152902A2 (de) 2007-06-08 2010-02-17 Epigenomics AG Verfahren zur methylierungsanalyse
US7811764B2 (en) * 2007-08-14 2010-10-12 University Of Rochester Hybridization-based biosensor containing hairpin probes and use thereof
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
EP2725107B1 (de) 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA-Sequenzierung mit nichtfluoreszierenden, reversiblen Nukleotidterminatoren und spaltbaren, fluoreszierenden ddNTPs und Nukleinsäuren die Inosine mit reversiblen Terminatorgruppe
US8957002B2 (en) 2007-11-05 2015-02-17 University Of Rochester DNA microarray having hairpin probes tethered to nanostructured metal surface
US20090197254A1 (en) * 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
KR100987352B1 (ko) * 2008-04-15 2010-10-12 주식회사 인트론바이오테크놀로지 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법
JP5757885B2 (ja) 2009-03-12 2015-08-05 ブランディーズ・ユニバーシティ Ｐｃｒ用の試薬および方法
JP5818018B2 (ja) * 2009-03-26 2015-11-18 厦門艾徳生物医薬科技有限公司 核酸増幅を用いる環状プライマー及びその応用
US8512955B2 (en) 2009-07-01 2013-08-20 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
ES2386514T3 (es) * 2009-07-10 2012-08-22 Biotrin International Limited Método isotérmico rápido, altamente sensible, para la detección de mutaciones puntuales y SNP
US8815515B1 (en) 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
US9176147B2 (en) 2009-10-29 2015-11-03 Wisconsin Alumni Research Foundation Detection of B-cell activating factor as a biomarker for antibody mediated rejection in transplant recipients
WO2011078441A1 (en) * 2009-12-21 2011-06-30 Seegene, Inc. Tsg primer target detection
EP2534263B1 (de) * 2010-02-09 2020-08-05 Unitaq Bio Verfahren und zusammensetzungen zur universellen erkennung von nukleinsäuren
CN103562405B (zh) 2010-11-10 2018-01-09 布兰代斯大学 用于检测和鉴定分枝杆菌属的组合物、方法和试剂盒
WO2012075231A1 (en) 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
AU2012242510B2 (en) 2011-04-15 2015-05-14 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
WO2013181553A2 (en) * 2012-06-01 2013-12-05 Akron Biotech Detection of mycoplasma in cell cultures and cell culture derived biologicals
CA2900259C (en) * 2013-02-07 2020-12-29 Rutgers, The State University Of New Jersey Highly selective nucleic acid amplification primers
US20140274779A1 (en) * 2013-03-15 2014-09-18 Abbott Molecular Inc. Multiplex allele detection
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
JP6412954B2 (ja) * 2014-04-29 2018-10-24 イルミナ インコーポレイテッド 鋳型切換え及びタグメンテーションを用いる単一細胞の遺伝子発現の多重分析
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
GB201416459D0 (en) * 2014-09-17 2014-10-29 Atlas Genetics Ltd Detection method
LT3957753T (lt) 2014-10-10 2025-01-10 Rutgers, The State University Of New Jersey Polimerazės grandininės reakcijos pradmenys ir zondai, skirti mycobacterium tuberculosis
KR101742681B1 (ko) 2015-01-30 2017-06-01 에스디 바이오센서 주식회사 상보적 염기서열 내지는 미스-매치된 염기를 포함하는 상보적인 염기서열과 연결된 pcr 프라이머 및 이를 이용한 핵산 증폭 방법
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
JP6681804B2 (ja) * 2016-03-30 2020-04-15 大研医器株式会社 変異プライマーの設計方法
CN106282353B (zh) * 2016-08-26 2019-12-10 上海翼和应用生物技术有限公司 一种利用发夹引物进行多重pcr的方法
AU2017366813B2 (en) * 2016-11-30 2023-04-20 Exosome Diagnostics, Inc. Methods and compositions to detect mutations in plasma using exosomal RNA and cell free DNA from non-small cell lung cancer patients
CN108517354A (zh) * 2017-02-28 2018-09-11 上海茂槿生物技术有限公司 用于多重pcr的引物组合物
JP7687957B2 (ja) 2019-03-14 2025-06-03 インシリクサ， インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム
CN109988758A (zh) * 2019-04-16 2019-07-09 上海快灵生物科技有限公司 一种参与聚合延伸的寡核苷酸链探针及其核酸扩增试剂盒
KR20220069111A (ko) 2019-10-02 2022-05-26 러트거즈,더스테이트유니버시티오브뉴저지 희귀 서열 변이체를 검출하기 위한 검정 방법 및 키트
CN111826421B (zh) * 2020-07-09 2021-09-21 广州迈景基因医学科技有限公司 一种pcr随机引物和使用其构建靶向测序文库的方法
CN116179655A (zh) * 2021-11-29 2023-05-30 北京迅识科技有限公司 一种实时荧光定量检测探针
CN113862339B (zh) * 2021-12-01 2022-03-22 广州滴纳生物科技有限公司 核酸组合产品、检测试剂盒和扩增靶核酸的方法
WO2024170770A1 (en) 2023-02-16 2024-08-22 Asecud Sa High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule
EP4488384A1 (de) * 2023-07-05 2025-01-08 Infiniplex Ltd. Primer zur selektiven amplifikation seltener zielsequenzen

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5503979A (en) 1984-05-25 1996-04-02 The Trustees Of Columbia University In The City Of New York Method of using replicatable hybridzable recombinant RNA probes
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
JP3898228B2 (ja) 1996-04-12 2007-03-28 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク 検出プローブ、キット及びアッセイ
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6037130A (en) 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits

Also Published As

Publication number Publication date
ATE394410T1 (de) 2008-05-15
EP1185546B1 (de) 2008-05-07
JP3843215B2 (ja) 2006-11-08
CA2375027C (en) 2010-07-27
US6365729B1 (en) 2002-04-02
EP1185546A1 (de) 2002-03-13
EP1185546A4 (de) 2002-08-21
AU4693900A (en) 2000-12-12
AU769219B2 (en) 2004-01-22
CA2375027A1 (en) 2000-11-30
WO2000071562A1 (en) 2000-11-30
US6277607B1 (en) 2001-08-21
JP2003500038A (ja) 2003-01-07

Similar Documents

Publication Publication Date Title
ATE394410T1 (de) Hochspezifische primer, amplifizierungsverfahren und kits
BR0014182A (pt) Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas
EP0774518A3 (de) Nukleinsäure-Sonden complementär zu Nukleinsäure von humane Papillomavirus, entsprechende Verfahren und Kits
WO2002052031A3 (en) Nucleic acid amplification
WO2002018641A3 (en) Detection of cyp3a4 and cyp2c9 polymorphisms
JP2003524416A5 (de)
JP7012957B2 (ja) Ｒｎａ配列の簡便検出法
ATE280177T1 (de) Modifizierte primer
EP1845160A4 (de) Nukleotidsequenz-amplifikationsverfahren
DK1419255T3 (da) En ny gruppe af alfa-amylaser såvel som en fremgangsmåde til at identificere og udvinde nye alfa-amylaser.
WO2009135093A3 (en) Rnase-h-based assays utilizing modified rna monomers
WO2006026388A3 (en) Single-primer nucleic acid amplification methods
AU2022295A (en) Nucleic acid amplification with DNA-dependant RNA polymerase activity of RNA replicases
EP2366803A3 (de) Zusammensetzungen und Verfahren zum Nacheis des West Nil Viruses.
EP1212449A4 (de) Verfahren zur amplifikation von signal-flankierenden sequenzen aus unbekannten, genomischen daten
RU98100921A (ru) Праймеры для обнаружения вич-1
WO1996006949A3 (en) Nucleic acid hybridization assay probes targeted to mycoplasma pneumoniae nucleic acid
ATE346169T1 (de) Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen
DK1026261T3 (da) Forögelse af specificiteten af nukleinsyreamplifikation med bærernukleinsyre
PT1171634E (pt) Pares de iniciadores de amplificacao e de sequenciacao e sua utilizacao
JP2006521824A5 (de)
BR0316224A (pt) Sequência de dna da região genÈmica tcd de photorhabdus luminescens
EP1193313A4 (de) Verfahren zur subtypenbestimmung von hiv-1
DE602004021094D1 (de) Gewinnung von dna
WO2003006065A3 (de) Pna-konjugat zur therapie von mit hiv in zusammenhang stehenden erkrankungen

Legal Events

Date Code Title Description
8364 No opposition during term of opposition