CO4520252A1 - Hexosa oxidasa recombinante - Google Patents
Hexosa oxidasa recombinanteInfo
- Publication number
- CO4520252A1 CO4520252A1 CO96028893A CO96028893A CO4520252A1 CO 4520252 A1 CO4520252 A1 CO 4520252A1 CO 96028893 A CO96028893 A CO 96028893A CO 96028893 A CO96028893 A CO 96028893A CO 4520252 A1 CO4520252 A1 CO 4520252A1
- Authority
- CO
- Colombia
- Prior art keywords
- tyr
- gly
- asp
- glu
- polypeptide
- Prior art date
Links
- 108010018734 hexose oxidase Proteins 0.000 title abstract 5
- 229920001184 polypeptide Polymers 0.000 abstract 9
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 9
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 9
- 108020004414 DNA Proteins 0.000 abstract 5
- 239000012634 fragment Substances 0.000 abstract 5
- 230000000694 effects Effects 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 4
- 230000000813 microbial effect Effects 0.000 abstract 4
- 235000013305 food Nutrition 0.000 abstract 3
- 102000053602 DNA Human genes 0.000 abstract 2
- 108020004511 Recombinant DNA Proteins 0.000 abstract 2
- 150000001413 amino acids Chemical group 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000004459 forage Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B2/00—Preservation of foods or foodstuffs, in general
- A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
- A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
- A23B2/729—Organic compounds; Microorganisms; Enzymes
- A23B2/783—Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/24—Synthetic spices, flavouring agents or condiments prepared by fermentation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/86—Products or compounds obtained by genetic engineering
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Nutrition Science (AREA)
- Animal Husbandry (AREA)
- Biomedical Technology (AREA)
- Birds (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Edible Seaweed (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Un método para producir un polipéptido que tiene actividadhexosa oxidasa, que comprende aislar o sintetizar un fragmento de DNA que codifica el polipéptido, introducir dicho fragmento de DNA en un organismo huésped apropiado en donde el fragmento de DNA es combinado con una señal de expresión apropiada para el fragmento de DNA, cultivar el organismo huésped bajo condiciones que llevan a la expresión del polipéptido con actividad hexosa oxidasa y recuperar el polipéptido del medio de cultivo o del organismo huésped.Un polipéptido en forma aislada que tiene actividad hexosa oxidasa, que comprende por lo menos una secuencia de aminoácidos seleccionada entre el grupo compuesto por:(i) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro(ii) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser- Gly-Tyr-Asp-X-X-X-Gly-Tyr-X-Val-Ser-Ser,(iii) Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser-Asn-Leu-X-Phe,(iv) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His-Thr,(v) Tyr-Tyr-Phe-Lys,(vi) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly-Thr-X-Asp,(vii) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp,(viii) X-Ile-Arg-Asp-Phe-Tyr-Glu-Glu-Met,en donde X representa un aminoácido seleccionado entre el grupo compuesto por Ala, Arg, Asn, Asp, Asx, Cys, Gln, Glu, Glx, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr y Val,y muteínas y variantes de los mismos.Una molécula de DNA recombinante que comprende un fragmento de DNA que codifica un polipéptido que tiene actividad hexosa oxidasa.Una célula microbiana que comprende la molécula de DNA recombinante de acuerdo con la reivindicación 26. Un método para preparar un producto alimenticio caracterizado porque se usa un polipéptido de acuerdo con la reivindicación 9 o una célula microbiana de acuerdo con la reivindicación 29.Un método para preparar un forraje para animales, caracterizado porque se usa el polipéptido de acuerdo con la reivindicación 9 o una célula microbiana de acuerdo con la reivindicación 29.Un método para reducir el contenido de azúcar de un producto alimenticio que comprende agregar a dicho producto una cantidad del polipéptido de acuerdo con la reivindicación 9 o una célula microbiana de acuerdo con la reinvindicación 29 que es suficiente para eliminar por lo menos parte del azúcar inicialmente presente en dicho producto alimenticio.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US47691095A | 1995-06-07 | 1995-06-07 |
Publications (1)
Publication Number | Publication Date |
---|---|
CO4520252A1 true CO4520252A1 (es) | 1997-10-15 |
Family
ID=23893752
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CO96028893A CO4520252A1 (es) | 1995-06-07 | 1996-06-04 | Hexosa oxidasa recombinante |
Country Status (21)
Country | Link |
---|---|
US (4) | US6251626B1 (es) |
EP (2) | EP1020523A3 (es) |
JP (1) | JP3917183B2 (es) |
CN (1) | CN1266275C (es) |
AR (2) | AR002301A1 (es) |
AT (1) | ATE223489T1 (es) |
AU (1) | AU705562B2 (es) |
BR (1) | BRPI9609230B1 (es) |
CA (1) | CA2224143C (es) |
CO (1) | CO4520252A1 (es) |
DE (1) | DE69623473T3 (es) |
DK (1) | DK0832245T4 (es) |
ES (1) | ES2182986T5 (es) |
MY (1) | MY116524A (es) |
NO (1) | NO324271B1 (es) |
NZ (1) | NZ310420A (es) |
PH (1) | PH11996053280B1 (es) |
PL (1) | PL187218B1 (es) |
TW (1) | TW438887B (es) |
WO (1) | WO1996040935A1 (es) |
ZA (1) | ZA964616B (es) |
Families Citing this family (55)
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JP3917183B2 (ja) | 1995-06-07 | 2007-05-23 | ダニスコ エー/エス | 組換えヘキソースオキシダーゼ、該酵素を産生する方法、および該酵素の利用 |
DK0833563T4 (da) | 1995-06-07 | 2008-06-16 | Danisco | En metode for at forberede egenskaberne for meldej |
GB9913050D0 (en) * | 1999-06-04 | 1999-08-04 | Danisco | Anti-fouling composition |
US20050287250A1 (en) * | 1995-06-07 | 2005-12-29 | Danisco A/S | Method |
US8178090B2 (en) | 1995-06-07 | 2012-05-15 | Danisco A/S | Recombinant hexose oxidase |
US7745599B1 (en) | 1995-06-07 | 2010-06-29 | Danisco A/S | Hexose oxidase-encoding DNAs and methods of use thereof |
US6936289B2 (en) * | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
ES2227730T3 (es) * | 1996-09-04 | 2005-04-01 | Syngenta Mogen B.V. | Proteinas antifungicas, adn que codifica para las mismas y hospedadores que lo incorporan. |
ATE220502T1 (de) | 1997-04-09 | 2002-08-15 | Danisco | Verbessertes verfahren zur herstellung von mehlteigwaren sowie produkte hergestellt aus solchen teigwaren unter verwendung von glyzerinoxidase |
ES2241189T3 (es) * | 1997-12-22 | 2005-10-16 | Novozymes A/S | Carbohidrato oxidasa y su uso para la coccion. |
DK1098988T4 (da) | 1998-07-21 | 2007-09-03 | Danisco | Födevare |
EP1008651A3 (en) * | 1998-12-09 | 2000-06-21 | Bioteknologisk Institut | Modified DNA sequence coding for hexose oxidase and use hereof |
GB9927801D0 (en) * | 1999-11-24 | 2000-01-26 | Danisco | Method |
US7455990B2 (en) * | 1999-11-24 | 2008-11-25 | Danisco A/S | Method of extracting recombinant hexose oxidase |
MXPA03004353A (es) * | 2000-11-17 | 2003-08-19 | Danisco | Procedimiento para la prevencion o reduccion de reaccion de maillard en un producto alimenticio. |
GB0028119D0 (en) | 2000-11-17 | 2001-01-03 | Danisco | Method |
US8163317B2 (en) | 2000-11-17 | 2012-04-24 | Danisco A/S | Method |
WO2002061068A2 (en) * | 2001-01-31 | 2002-08-08 | Novozymes A/S | Oxidase free of catalase side activities |
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US7550647B2 (en) | 2001-09-14 | 2009-06-23 | Advanced Bionutrition | Transfected shrimp as production systems for therapeutic proteins |
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US7955814B2 (en) | 2003-01-17 | 2011-06-07 | Danisco A/S | Method |
US20040241283A1 (en) * | 2003-05-28 | 2004-12-02 | Domingues David J. | Method of preventing discoloration of dough, dough compositions, and dough products |
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CN102307956B (zh) | 2009-02-06 | 2014-12-24 | 汉伯公司 | 基于酶的自抛光涂料组合物 |
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CN110790823B (zh) * | 2019-12-04 | 2021-02-09 | 江南大学 | 一种解淀粉芽孢杆菌产抑菌活性物质的方法 |
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EP1108360A1 (en) | 1999-12-03 | 2001-06-20 | Danisco A/S | Method of improving dough and bread quality |
WO2002000852A2 (en) | 2000-06-26 | 2002-01-03 | Novozymes A/S | Lipolytic enzyme |
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1996
- 1996-06-04 JP JP50009097A patent/JP3917183B2/ja not_active Expired - Lifetime
- 1996-06-04 MY MYPI96002159A patent/MY116524A/en unknown
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- 1996-06-04 CO CO96028893A patent/CO4520252A1/es unknown
- 1996-06-04 DE DE69623473T patent/DE69623473T3/de not_active Expired - Lifetime
- 1996-06-04 AU AU61215/96A patent/AU705562B2/en not_active Expired
- 1996-06-04 AT AT96918616T patent/ATE223489T1/de active
- 1996-06-04 US US08/669,304 patent/US6251626B1/en not_active Expired - Lifetime
- 1996-06-04 PH PH11996053280A patent/PH11996053280B1/en unknown
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- 1996-06-04 WO PCT/DK1996/000238 patent/WO1996040935A1/en active IP Right Grant
- 1996-06-04 EP EP00101681A patent/EP1020523A3/en not_active Withdrawn
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