CN119453279A

CN119453279A - Biological bacteriostat

Info

Publication number: CN119453279A
Application number: CN202411571642.7A
Authority: CN
Inventors: 韦庆文; 陈丽丽; 孟家庆; 潘长沛; 农桂高; 黄荣武
Original assignee: Guangxi Baishengyuan Agricultural Technology Co ltd
Current assignee: Guangxi Baishengyuan Agricultural Technology Co ltd
Priority date: 2024-11-06
Filing date: 2024-11-06
Publication date: 2025-02-18

Abstract

The invention discloses a biological bacteriostat, which belongs to the technical field of biological bacteriostats and comprises the following components of gaseous available chlorine, an oxalis extract, sericin and trehalose, wherein the preparation method not only can sterilize agricultural products, but also can keep fresh the agricultural products and improve the taste and nutrition of the agricultural products, and meanwhile, the biological bacteriostat is free of toxic and harmful effects, and the cleaning residues can not cause damage to human health after being eaten, and meanwhile, the preparation processes of the gaseous available chlorine, the oxalis extract and the sericin supplement each other, namely, the product of the gaseous available chlorine can be used for preparing the biological bacteriostat and the oxalis extract, the heat generation of the oxalis extract can be used for preparing the sericin, the expenditure of the preparation cost can be reduced to the greatest extent, the preparation yield is improved, and the biological bacteriostat has wide market competitiveness.

Description

Biological bacteriostat

Technical Field

The invention belongs to the technical field of biological bacteriostats, and particularly relates to a biological bacteriostats.

Background

After picking or harvesting, if the agricultural products cannot be properly preserved, the agricultural products are easy to be polluted by microorganisms, so that the agricultural products are rotten and deteriorated, the taste and the nutritive value are greatly reduced, harmful substances are possibly generated, and the agricultural products are threatening to human health.

The existing agricultural product preservation method generally comprises spraying chemicals such as preservative to inhibit the activities of microorganisms and enzymes, and the method has the following problems that the chemicals can not be completely removed in the agricultural product cleaning stage and can remain on the surface of the agricultural product, and the human health can be influenced after eating the agricultural product.

In view of this, a biological bacteriostat is designed to solve the above-mentioned problems.

Disclosure of Invention

To solve the problems set forth in the background art. The biological bacteriostat provided by the invention has the characteristics of sterilizing agricultural products, keeping fresh of the agricultural products, improving the taste and nutrition of the agricultural products, having no toxic or harmful effect, avoiding damaging human health after cleaning residues are eaten, reducing the expenditure of preparation cost to the greatest extent, improving the preparation yield and having wide market competitiveness.

In order to achieve the aim, the invention provides the following technical scheme that the biological bacteriostat comprises the following components:

gaseous available chlorine, oxalis extract, sericin and trehalose;

The gaseous effective chlorine is prepared by injecting saturated saline water into an electrolytic tank to perform electrolytic reaction to generate sodium hydroxide solution, chlorine and hydrogen, and separating and purifying the sodium hydroxide solution, the chlorine and the hydrogen, wherein the chlorine is the gaseous effective chlorine;

The method comprises the steps of reacting carbon monoxide with separated and purified hydrogen to generate methanol, picking fresh oxalis, cleaning, drying, crushing, placing crushed oxalis slag and generated methanol in a solvent container, immersing the oxalis slag with the methanol, heating the solvent container, heating the oxalis slag and the methanol, evaporating the methanol to form vapor, rising the vapor to a condensing tube, cooling and refluxing to the solvent container until the heating time is up, stopping heating, filtering the extracting solution in the solvent container to obtain a methanol extracting solution of quercetin, and separating and purifying the quercetin by using DM-130 adsorption resin to obtain the quercetin, namely the oxalis extract;

Cutting silk cocoons with high quality into small blocks by scissors, washing with deionized water, removing surface impurities, mixing with a separated and purified sodium hydroxide solution, preheating by utilizing waste heat of a condenser pipe, then reheating by a heater, stirring and mixing, waiting for the silk cocoons to dissolve in the sodium hydroxide solution until the silk cocoons are completely dissolved, filtering the mixture to remove insoluble impurities, centrifuging to separate silk gum solution, and freeze-drying to remove water of the silk gum solution;

The trehalose is prepared by inoculating lactobacillus to a slant culture medium for activation culture, inoculating to a shake flask culture medium for shake flask culture to enable the strain to reach logarithmic phase, inoculating to a fermentation tank for fermentation, centrifuging and purifying.

The preparation method of the biological bacteriostat comprises the following steps:

S1, introducing gaseous available chlorine into a separated and purified sodium hydroxide solution or purified water to adjust the pH value until the pH value reaches 8.5-9.5;

S2, placing the oxalis extract, sericin and trehalose which have the volume of 1:1 with the gaseous effective chlorine into the solution, and stirring and mixing until the solution is uniformly mixed to prepare the biological bacteriostat.

Further, in the step S1, the specific steps of gaseous available chlorine include:

s101, designing and building an electrolytic tank, wherein the electrolytic tank has the functions of adjusting current, voltage and temperature parameters, and an anode and a cathode are arranged in the electrolytic tank and are connected with an external power supply through wires;

S102, injecting saturated saline water into an electrolytic tank, switching on a power supply, enabling current to flow between an anode and a cathode to trigger electrolytic reaction, enabling oxidation reaction to occur on the anode, enabling reduction reaction to occur on the cathode, and generating sodium hydroxide solution, chlorine and hydrogen, wherein the chlorine is gaseous effective chlorine.

Further, in the step S1, the sodium hydroxide solution is the sodium hydroxide solution separated and purified in the step S102.

Further, in the step S2, the specific steps of the oxalis extract include:

S201, building a reaction system structure, wherein the reaction system structure comprises a solvent container A, a heater A, a condensing pipe and a cooler, the solvent container A is arranged in the heater A, the condenser is arranged above the solvent container A and fully covers an opening of the solvent container A, the cooler covers the condensing pipe, and the condensing pipe is arranged above the solvent container A;

s202, picking fresh oxalis, cleaning, drying and placing in a crushing container for crushing;

s203, reacting carbon monoxide with the hydrogen separated and purified in the step S102 to generate methanol;

s204, placing the crushed oxalis residues and methanol in a solvent container A to enable the methanol to submerge the oxalis residues;

S205, heating by a heater A, evaporating the oxalis slag and the methanol to form vapor in the heating process, rising the vapor to a condensing pipe, cooling the vapor into liquid in the condensing pipe under the action of a cold source provided by a cooler, and refluxing the liquid to a solvent container A;

S206, stopping heating when the set reaction time is reached, and placing the extracting solution in the solvent container A in a filtering container to filter out residues to obtain a methanol extracting solution of quercetin;

S207, separating and purifying quercetin by DM-130 adsorption resin to obtain herba Oxalidis Corniculatae extract.

Further, in the step S2, the specific steps of sericin include:

S208, constructing a reaction system structure, wherein the reaction system structure comprises a solvent container B and a heater B, a condensing pipe in the step S201 is wound on the outer wall of the solvent container B, a cooler is arranged at the tail end of the condensing pipe, and the solvent container B is arranged in the heater B;

S209, preparing multiplied cocoons;

s210, cutting cocoons into small pieces through scissors;

s211, cleaning small cocoons with deionized water to remove surface impurities;

S212, placing the cleaned small cocoons in a solvent container B, adding the sodium hydroxide solution separated and purified in the step S102, preheating by the residual heat of steam in a wound condensing tube, heating by a heater B, stirring and mixing in the heating process, and waiting for the cocoons to be dissolved in the sodium hydroxide solution;

S213, after complete dissolution, placing the mixture in a filter container to filter out undissolved impurities;

s214, placing the filtrate into a centrifugal processor for centrifugal treatment, and separating out sericin solution;

And S215, placing the sericin solution in a freeze dryer for freeze drying, and removing the moisture in the sericin solution to obtain sericin.

Further, in the step S2, the specific steps of trehalose include:

s216, inoculating lactobacillus to a slant culture medium for activation culture;

S217, inoculating the activated strain into a shake flask culture medium from a slant culture medium, and culturing in a shake flask to enable the strain to reach a logarithmic phase;

S218, inoculating bacterial liquid obtained by shake flask culture into a fermentation tank for fermentation;

S219, after fermentation is finished, placing the fermentation liquor into a centrifugal machine for centrifugal treatment to obtain fermentation liquor;

s220, pumping the fermentation liquor into an ion exchange column, exchanging trehalose in the fermentation liquor with ions on ion exchange resin in the ion exchange column, eluting the ion exchange column through eluent, eluting trehalose from the ion exchange resin, and collecting eluent, namely trehalose solution.

Compared with the prior art, the invention has the beneficial effects that:

The invention is prepared from gaseous effective chlorine, an oxalis extract, sericin and trehalose, wherein the gaseous effective chlorine has a disinfection effect, the oxalis extract has an antioxidation effect, the sericin has an antioxidation effect, a hydration film can be formed after spraying, the effects of moisturizing, preserving and forming a natural barrier to inhibit diseases and insect pests are achieved, the trehalose prepared by microorganisms has the effects of preserving water and improving nutrition, the combination of the four can sterilize agricultural products, preserve the agricultural products and improve the mouthfeel and nutrition of the agricultural products, the toxic effect is avoided, the cleaning residues are not harmful to human health after being eaten, and meanwhile, the preparation processes of the gaseous effective chlorine, the oxalis extract and the sericin supplement each other, namely the product of the gaseous effective chlorine can be used for preparing biological bacteriostat and preparing the oxalis extract, the heat production of the oxalis used for preparing the sericin, the preparation cost can be reduced to the maximum extent, the preparation yield is improved, and the wide market competitiveness is achieved.

Drawings

FIG. 1 is a flow chart of a preparation method of the biological bacteriostat.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

A biological bacteriostat, comprising the following components:

gaseous available chlorine, oxalis extract, sericin and trehalose;

Referring to fig. 1, a preparation method of the biological bacteriostat comprises the following steps:

In the embodiment, the addition ratio of the gaseous available chlorine and the sodium hydroxide solution or the purified water is that the PH value of the mixed solution is adjusted to 8.5-9.5;

Specifically, in step S1, the specific steps of gaseous available chlorine include:

In this embodiment, the chemical reaction equation is as follows:

2NaCl+2H₂O＝2NaOH+Cl₂↑+H₂↑

The addition ratio of NaCl and H ₂ O in the saturated saline water can meet the completion of the chemical reaction equation;

The gaseous available chlorine can rapidly inhibit various bacteria such as escherichia coli, staphylococcus aureus, candida albicans and the like, and bacterial silkworm pathogens such as silkworm nuclear polyhedrosis virus, cytoplasmic polyhedrosis virus, concentrated nuclear disease virus, malacia virus and the like.

Specifically, in step S1, the sodium hydroxide solution is the sodium hydroxide solution separated and purified in step S102.

Specifically, in step S2, the specific steps of the oxalis extract include:

In this embodiment, the chemical reaction equation is as follows:

CO↑+2H₂↑＝CH₃OH

In this embodiment, the addition ratio of CO and H ₂ may be satisfied by the completion of the chemical reaction equation;

Quercetin has strong antioxidant and antibacterial effects, and can prolong storage period, reduce weight loss and pathological change of agricultural products during storage, slow down the decrease of soluble solid, total acid and total sugar content, protect VC, maintain CAT and SOD activity, reduce POD activity, and delay fruit tissue aging.

Specifically, in step S2, the specific steps of sericin include:

S209, preparing multiplied cocoons;

s210, cutting cocoons into small pieces through scissors;

s211, cleaning small cocoons with deionized water to remove surface impurities;

The sericin has strong oxidation and disproportionation resistance, can prevent the attack of active oxygen generated in the metabolic process, effectively maintains the integrity of the film structure, has the functions of absorbing water and preserving moisture, can form a layer of hydration film when the aqueous solution of the sericin is sprayed on the surface of agricultural products, is like a natural barrier, can effectively inhibit the occurrence of plant diseases, and has the function of preserving freshness.

Specifically, in step S2, the specific steps of trehalose include:

In the embodiment, the shake flask culture temperature is 30 ℃, the shake flask culture rotating speed is 140r/min, and the shake flask culture time is 20h;

in this example, the inoculation amount of the bacterial liquid is 10%;

The hydroxyl groups in the molecule of the trehalose are all hydrophilic ethane, so that the moisture of the aqueous solution can be effectively locked, the water retention effect is realized, meanwhile, the trehalose is a nutritional substance which is easily absorbed by the small intestine to become energy, and the trehalose is nontoxic and can provide the nutritional substance for human bodies after residues.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A biological antibacterial agent, characterized in that it comprises the following components:

Gaseous available chlorine, Oxalis extract, sericin and trehalose;

Gaseous available chlorine is obtained by injecting saturated salt water into an electrolytic cell for electrolysis to generate sodium hydroxide solution, chlorine gas and hydrogen gas. The sodium hydroxide solution, chlorine gas and hydrogen gas are separated and purified to obtain chlorine gas, which is gaseous available chlorine.

The Oxalis extract is prepared by reacting carbon monoxide with separated and purified hydrogen to generate methanol, fresh Oxalis is picked, washed, dried, and crushed, the crushed Oxalis residue and the generated methanol are placed in a solvent container, the Oxalis residue is immersed in methanol, the solvent container is heated, the Oxalis residue and methanol are heated, the methanol evaporates to form steam, the steam rises to a condenser, cools and refluxes into the solvent container until the heating time is up, the heating is stopped, the extract in the solvent container is filtered, and a methanol extract of quercetin is obtained, and the quercetin is separated and purified by adsorption with DM-130 adsorption resin to obtain the quercetin, that is, the Oxalis extract;

Sericin is prepared by cutting high-quality silkworm cocoons into small pieces with scissors, washing with deionized water to remove surface impurities, mixing with separated and purified sodium hydroxide solution, preheating with waste heat from a condenser tube and then reheating with a heater, stirring and mixing, waiting for the silkworm cocoons to dissolve in the sodium hydroxide solution until they are completely dissolved, filtering the mixture to remove insoluble impurities, centrifuging to separate the sericin solution, and freeze-drying to remove moisture from the sericin solution;

Trehalose is prepared by inoculating lactobacillus onto a slant medium for activation culture, then inoculating it into a shake flask medium for shake flask culture to allow the strain to reach a logarithmic growth phase, then inoculating it into a fermenter for fermentation, and then centrifuging and purifying it.

2. The method for preparing a biological antibacterial agent according to claim 1, characterized in that it comprises the following steps:

S1: Pass gaseous available chlorine into separated and purified sodium hydroxide solution or pure water to adjust the pH value until the pH value reaches 8.5-9.5;

S2: Place Oxalis sorrel extract, sericin and trehalose in a volume ratio of 1:1 with gaseous available chlorine into the above solution, stir and mix until the mixture is uniform, and prepare a biological antibacterial agent.

3. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: in step S1, the specific steps of gaseous available chlorine include:

S101: Design and build an electrolytic cell with adjustable current, voltage and temperature parameters. Install anodes and cathodes in the electrolytic cell, and connect the anodes and cathodes to an external power source through wires;

S102: Inject saturated salt water into the electrolytic cell and turn on the power supply. The current flows between the anode and the cathode, triggering an electrolytic reaction. An oxidation reaction occurs at the anode and a reduction reaction occurs at the cathode, generating sodium hydroxide solution, chlorine gas and hydrogen gas. The chlorine gas is the gaseous available chlorine.

4. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: in the step S1, the sodium hydroxide solution is the sodium hydroxide solution separated and purified in step S102.

5. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: in the step S2, the specific steps of extracting Oxalis sorrel include:

S201: constructing a reaction system structure, including a solvent container A, a heater A, a condenser and a cooler, wherein the solvent container A is placed in the heater A, the condenser is placed above the solvent container A and fully covers the opening of the solvent container A, the cooler covers the condenser, and the condenser is located above the solvent container A;

S202: picking fresh Oxalis, washing, drying, and placing in a crushing container for crushing;

S203: reacting carbon monoxide with the hydrogen separated and purified in step S102 to generate methanol;

S204: placing the crushed Oxalis residue and methanol in a solvent container A, so that the methanol submerges the Oxalis residue;

S205: Heater A heats the Oxalis residue and methanol. During the heating process, methanol evaporates to form steam. The steam rises to the condenser. Under the action of the cooler providing a cold source, the steam is cooled into liquid in the condenser and flows back into the solvent container A.

S206: When the set reaction time is reached, the heating is stopped, and the extract in the solvent container A is placed in a filter container to filter out the residue to obtain a methanol extract of quercetin;

S207: quercetin, i.e., Oxalis sorrel extract, is separated and purified by adsorption using DM-130 adsorption resin.

6. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: in step S2, the specific steps of sericin protein include:

S208: constructing a reaction system structure, including a solvent container B and a heater B, wherein the condenser in step S201 is wound around the outer wall of the solvent container B, the cooler is placed at the end of the condenser, and the solvent container B is placed in the heater B;

S209: Prepare the finest silkworm cocoons;

S210: Cut the cocoons into small pieces using scissors;

S211: Clean the small pieces of silk cocoons with deionized water to remove surface impurities;

S212: placing the cleaned small pieces of silk cocoons in a solvent container B, adding the sodium hydroxide solution separated and purified in step S102, first preheating them with the residual heat of the steam in the wound condenser tube, and then heating them with the heater B, stirring and mixing during the heating process, and waiting for the silk cocoons to dissolve in the sodium hydroxide solution;

S213: After being completely dissolved, the mixture is placed in a filtering container to filter out insoluble impurities;

S214: placing the filtrate in a centrifugal processor for centrifugal treatment to separate the sericin solution;

S215: placing the sericin solution in a freeze dryer for freeze drying to remove moisture in the sericin solution and obtain sericin protein.

7. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: in step S2, the specific steps of trehalose include:

S216: inoculating lactobacillus onto the slant culture medium for activation culture;

S217: inoculating the activated strain from the slant culture medium into the shake flask culture medium, and culturing the strain in the shake flask until the strain reaches the logarithmic growth phase;

S218: inoculating the bacterial liquid obtained by the shake flask culture into a fermentation tank for fermentation;

S219: After the fermentation is completed, the fermentation liquid is placed in a centrifuge for centrifugation to obtain a fermentation liquid;

S220: pumping the fermentation liquid into the ion exchange column, the trehalose in the fermentation liquid is exchanged with the ions on the ion exchange resin in the ion exchange column, the ion exchange column is eluted with the eluent, the trehalose is eluted from the ion exchange resin, and the eluent, i.e., the trehalose solution, is collected.