CN119453281A - Biological agent with disinfection and fresh-keeping functions on mulberry leaves and preparation method thereof - Google Patents
Biological agent with disinfection and fresh-keeping functions on mulberry leaves and preparation method thereof Download PDFInfo
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- CN119453281A CN119453281A CN202411571616.4A CN202411571616A CN119453281A CN 119453281 A CN119453281 A CN 119453281A CN 202411571616 A CN202411571616 A CN 202411571616A CN 119453281 A CN119453281 A CN 119453281A
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- 235000008708 Morus alba Nutrition 0.000 title claims abstract description 44
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- 238000004659 sterilization and disinfection Methods 0.000 title abstract description 3
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims abstract description 98
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Abstract
本发明公开了一种对桑叶具有消毒及保鲜的生物制剂，属于生物制剂技术领域，包括以下组分：食品级二氧化氯、丝胶蛋白、酢浆草提取液和低聚果糖；本发明还公开了一种对桑叶具有消毒及保鲜的生物制剂的制备方法；本发明食品级二氧化氯具有消毒同时抑制多种细菌的作用，丝胶蛋白具有抗氧化性，同时在喷洒后可以在桑叶表面形成水化膜，起到保湿作用的同时起到抑制病虫害发生的作用，具有保鲜作用，酢浆草提取液具有保鲜作用，低聚果糖具有提升桑叶营养的作用，四者相结合，不仅能够对桑叶进行杀菌，而且能够对桑叶进行保鲜，提高桑叶的口感和营养，增加蚕的进食量，提高蚕的长速的同时提高蚕的疾病抵抗力。The invention discloses a biological preparation for disinfecting and preserving mulberry leaves, belongs to the technical field of biological preparations, and comprises the following components: food-grade chlorine dioxide, sericin, oxalis extract and oligofructose; the invention also discloses a preparation method of the biological preparation for disinfecting and preserving mulberry leaves; the food-grade chlorine dioxide of the invention has the functions of disinfection and inhibition of multiple bacteria, the sericin has antioxidant properties, and can form a hydration film on the surface of the mulberry leaves after spraying, which has the functions of moisturizing and inhibiting the occurrence of pests and diseases, and has the function of preserving; the oxalis extract has the function of preserving; the oligofructose has the function of improving the nutrition of the mulberry leaves, and the combination of the four can not only sterilize the mulberry leaves, but also preserve the mulberry leaves, improve the taste and nutrition of the mulberry leaves, increase the food intake of silkworms, and improve the growth rate of silkworms while improving the disease resistance of silkworms.
Description
Technical Field
The invention belongs to the technical field of biological agents, and in particular relates to a biological agent for sterilizing and preserving mulberry leaves and a preparation method thereof.
Background
Mulberry leaves are leaves of commercial mulberry, which are important raw materials for silkworm production, and yield and quality are directly related to the quality of cocoons and raw silks.
The traditional soil fertilization mode is often limited by factors such as climate and soil conditions, so that the annual nutrition requirement of the mulberry is difficult to meet, and the yield and quality of the mulberry are affected.
The Chinese patent application No. 201410298994.X discloses a preparation method of a preservative for oxalis extract, which is obtained by uniformly stirring starch acrylic acid copolymer hydrogel and oxalis extract with a volume ratio of 1:1, wherein the starch acrylic acid copolymer hydrogel is prepared by putting starch into a radiation field for pre-irradiation with a dose of 12 kilocuries, putting starch and acrylic acid with a mass ratio of 3:1 into a glass beaker according to the sequence of acrylic acid-starch, carrying out polymerization reaction at a certain stirring speed, adding a proper amount of sodium hydroxide solution into the beaker after the reaction is finished, uniformly stirring with a glass rod to neutralize the acidity of the product, picking fresh oxalis complete, cleaning, drying, pulverizing into powder, soaking the oxalis powder with a mass ratio of 1:10 in distilled water for 12 hours, decocting for 2 times at 80 ℃ each time for 1.5 hours, filtering while the filtrate is hot, and cooling to normal temperature.
The technology has the following problems that the biological agent can only keep the fresh of the mulberry leaves, and the fresh mulberry leaves can be affected by diseases and insect pests in the Sang Shezai growing process, so that the fresh mulberry leaves are more easily affected by the diseases and insect pests, and the service performance of the fresh mulberry leaves is reduced.
In view of this, a biological agent for sterilizing and preserving mulberry leaves and a preparation method thereof are designed to solve the problem that the biological agent is only preserved and not sterilized.
Disclosure of Invention
To solve the problems set forth in the background art. The invention provides a biological agent for sterilizing and preserving mulberry leaves and a preparation method thereof, which have the characteristics of sterilizing the mulberry leaves, preserving the mulberry leaves, improving the taste and nutrition of the mulberry leaves, increasing the feeding quantity of silkworms, improving the disease resistance of the silkworms while improving the growth speed of the silkworms.
The invention also aims to provide a preparation method of the biological agent for sterilizing and preserving mulberry leaves.
In order to achieve the aim, the invention provides the following technical scheme that the biological agent for sterilizing and preserving mulberry leaves comprises the following components:
food grade chlorine dioxide, sericin, oxalis extract and fructo-oligosaccharides;
The food-grade chlorine dioxide is prepared by preparing electrolyte from sea salt and water, injecting the electrolyte into an electrolytic tank, carrying out electrolytic reaction to generate sodium hydroxide solution, chlorine and hydrogen, separating and purifying the sodium hydroxide solution, the chlorine and the hydrogen, reacting the chlorine with sodium chlorite solution to generate food-grade chlorine dioxide gas and sodium chloride solution, mixing the food-grade chlorine dioxide gas with water, and adjusting the PH value to 8.5-9.5 through sodium hydroxide solution or dilution;
The generated sodium hydroxide solution and the food-grade chlorine dioxide solution react with the hydrogen peroxide solution to generate sodium chlorite solution, oxygen and water, the generated sodium chlorite solution supplements the sodium chlorite solution reacted with chlorine, the generated hydrogen and oxygen react with ethyl anthraquinone to generate hydrogen peroxide solution, the generated hydrogen peroxide solution supplements the hydrogen peroxide solution reacted with the sodium hydroxide solution and the food-grade chlorine dioxide solution, the generated sodium chloride solution can supplement sodium chloride of sea salt part in the electrolyte, and the generated water can supplement water in the electrolyte;
Cutting silk fibroin into small pieces by scissors, cleaning with deionized water, removing surface impurities, mixing with sodium hydroxide solution under stirring, standing for silk cocoon dissolution, filtering the mixture to remove insoluble impurities, centrifuging to separate silk gelatin solution, and lyophilizing to remove water content of silk gelatin solution;
The extractive solution is prepared by cleaning and oven drying fresh herba Oxalidis Corniculatae, pulverizing into powder, soaking in distilled water, decocting twice, filtering while hot, mixing the filtrates, and cooling to room temperature;
the fructo-oligosaccharide is prepared by mixing sucrose with water to generate a sucrose solution, inoculating Aspergillus niger into the sucrose solution for fermentation, catalyzing sucrose by FTS enzyme generated in the fermentation process to generate fructo-oligosaccharide, filtering to separate fructo-oligosaccharide, adsorbing pigment of fermentation liquor adhered to the fructo-oligosaccharide by an activated carbon column, adsorbing salt of the fermentation liquor adhered to the fructo-oligosaccharide by an ion exchange resin, and removing water adhered to the fructo-oligosaccharide by freeze drying.
The preparation method of the biological agent for sterilizing and preserving mulberry leaves comprises the following steps:
Mixing food-grade chlorine dioxide, sericin, oxalis extract and fructo-oligosaccharide in a volume ratio of 1:1:1:1, stirring until the components are uniformly mixed, and obtaining the biological preparation for sterilizing and preserving mulberry leaves.
Further, the preparation steps of the food-grade chlorine dioxide comprise:
s101, designing and building an electrolytic tank, wherein the electrolytic tank has the functions of adjusting current, voltage and temperature parameters, and an anode and a cathode are arranged in the electrolytic tank and are connected with an external power supply through wires;
S102, mixing sea salt and purified water according to a proportion to form an electrolyte solution, and injecting the electrolyte solution into an electrolytic tank;
s103, switching on a power supply;
S104, current flows between the anode and the cathode to trigger electrolytic reaction, oxidation reaction occurs on the anode, reduction reaction occurs on the cathode to generate sodium hydroxide solution, chlorine and hydrogen;
s105, separating and purifying sodium hydroxide solution, chlorine and hydrogen generated by electrolysis;
S106, reacting the purified chlorine gas with sodium chlorite solution to generate food-grade chlorine dioxide gas and sodium chloride solution;
s107, separating and purifying the food-grade chlorine dioxide gas and sodium chloride solution generated by the reaction, and supplementing sea salt components in the step S102 by the purified sodium chloride solution;
s108, introducing purified food-grade chlorine dioxide gas into water, mixing with the water, and detecting the PH value;
And S109, introducing purified sodium hydroxide solution or water into the reactor to adjust the pH value to 8.5-9.5 according to the detected pH value.
Further, the step of preparing the food-grade chlorine dioxide further comprises:
s110, reacting the sodium hydroxide solution purified in the step S105 and the food-grade chlorine dioxide gas purified in the step S107 with hydrogen peroxide solution under the condition of surplus to generate sodium chlorite solution, oxygen and water;
And S111, separating and purifying the sodium chlorite solution, oxygen and water generated by the reaction, wherein the purified sodium chlorite solution supplements the sodium chlorite solution in the step S106, and the purified water supplements the purified water in the step S102.
Further, the step of preparing the food-grade chlorine dioxide further comprises:
s112, reacting the hydrogen purified in the step S105 and the oxygen purified in the step S111 with ethyl anthraquinone to generate hydrogen peroxide solution;
And S113, separating and purifying the hydrogen peroxide solution generated by the reaction, and supplementing the hydrogen peroxide solution in the step S110 with the purified hydrogen peroxide solution.
Further, the preparation method of the sericin comprises the following steps:
s201, preparing multiplied cocoons;
s202, cutting cocoons into small pieces through scissors;
s203, cleaning small cocoons with deionized water to remove surface impurities;
S204, placing the cleaned small cocoons in a heating container, adding sodium hydroxide solution, heating and stirring to dissolve the cocoons in the sodium hydroxide solution, and filtering to remove insoluble impurities;
s205, centrifuging the filtered mixture to separate sericin solution;
S206, freeze-drying the sericin solution to remove part of water.
Further, the preparation method of the oxalis extract comprises the following steps:
s301, picking fresh whole herb of oxalis;
s302, cleaning and drying picked fresh whole herb of oxalis, and removing surface impurities;
s303, placing the dried oxalis mother grass in a crusher for crushing, and pulverizing into powder;
s304, soaking and steaming the oxalis powder in distilled water twice, filtering out filtrate, combining the filtrates, and cooling to room temperature.
Further, the preparation method of the fructooligosaccharide comprises the following steps:
S401, placing sucrose in water and mixing with the water to generate a sucrose solution;
S402, inoculating Aspergillus niger into a sucrose solution for fermentation, wherein FTS enzyme generated in the fermentation process catalyzes sucrose to perform a transfer reaction, so that fructo-oligosaccharide is generated;
s403, filtering the produced fructo-oligosaccharide to separate the fructo-oligosaccharide;
S404, adsorbing pigment of fermentation liquor adhered on fructo-oligosaccharide through an activated carbon column;
S405, adsorbing the salt of the fermentation broth adhered to the fructo-oligosaccharide by ion exchange resin;
S406, removing the adhered moisture on the fructo-oligosaccharide by freeze drying.
Compared with the prior art, the invention has the beneficial effects that:
1. The invention is prepared from food-grade chlorine dioxide, sericin, an oxalis extract and fructo-oligosaccharide, wherein the food-grade chlorine dioxide has the effects of sterilizing and inhibiting various bacteria, the sericin has the oxidation resistance, a hydration film can be formed on the surface of mulberry leaves after spraying, the moisturizing effect is achieved, the insect diseases are inhibited, the fresh-keeping effect is achieved, the oxalis extract has the fresh-keeping effect, the fructo-oligosaccharide has the effect of improving the nutrition of the mulberry leaves, and the combination of the four has the effects of sterilizing the mulberry leaves, keeping fresh the mulberry leaves, improving the taste and nutrition of the mulberry leaves, increasing the feeding amount of silkworms, and improving the disease resistance of the silkworms while improving the long speed of the silkworms.
2. The substances generated in the reaction process of the reaction substances added in the food-grade chlorine dioxide preparation process can realize integral recycling, so that the cost expenditure of manufacturers on the reaction substances can be reduced, and the benefit effect of the manufacturers is improved.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides the following technical scheme that the biological agent for sterilizing and preserving mulberry leaves comprises the following components:
food grade chlorine dioxide, sericin, oxalis extract and fructo-oligosaccharides;
The food-grade chlorine dioxide is prepared by preparing electrolyte from sea salt and water, injecting the electrolyte into an electrolytic tank, carrying out electrolytic reaction to generate sodium hydroxide solution, chlorine and hydrogen, separating and purifying the sodium hydroxide solution, the chlorine and the hydrogen, reacting the chlorine with sodium chlorite solution to generate food-grade chlorine dioxide gas and sodium chloride solution, mixing the food-grade chlorine dioxide gas with water, and adjusting the PH value to 8.5-9.5 through sodium hydroxide solution or dilution;
The generated sodium hydroxide solution and the food-grade chlorine dioxide solution react with the hydrogen peroxide solution to generate sodium chlorite solution, oxygen and water, the generated sodium chlorite solution supplements the sodium chlorite solution reacted with chlorine, the generated hydrogen and oxygen react with ethyl anthraquinone to generate hydrogen peroxide solution, the generated hydrogen peroxide solution supplements the hydrogen peroxide solution reacted with the sodium hydroxide solution and the food-grade chlorine dioxide solution, the generated sodium chloride solution can supplement sodium chloride of sea salt part in the electrolyte, and the generated water can supplement water in the electrolyte;
Cutting silk fibroin into small pieces by scissors, cleaning with deionized water, removing surface impurities, mixing with sodium hydroxide solution under stirring, standing for silk cocoon dissolution, filtering the mixture to remove insoluble impurities, centrifuging to separate silk gelatin solution, and lyophilizing to remove water content of silk gelatin solution;
The extractive solution is prepared by cleaning and oven drying fresh herba Oxalidis Corniculatae, pulverizing into powder, soaking in distilled water, decocting twice, filtering while hot, mixing the filtrates, and cooling to room temperature;
the fructo-oligosaccharide is prepared by mixing sucrose with water to generate a sucrose solution, inoculating Aspergillus niger into the sucrose solution for fermentation, catalyzing sucrose by FTS enzyme generated in the fermentation process to generate fructo-oligosaccharide, filtering to separate fructo-oligosaccharide, adsorbing pigment of fermentation liquor adhered to the fructo-oligosaccharide by an activated carbon column, adsorbing salt of the fermentation liquor adhered to the fructo-oligosaccharide by an ion exchange resin, and removing water adhered to the fructo-oligosaccharide by freeze drying.
A preparation method of a biological agent for sterilizing and preserving mulberry leaves comprises the following steps:
Mixing food-grade chlorine dioxide, sericin, oxalis extract and fructo-oligosaccharide in a volume ratio of 1:1:1:1, stirring until the components are uniformly mixed, and obtaining the biological preparation for sterilizing and preserving mulberry leaves.
Specifically, the preparation steps of the food-grade chlorine dioxide comprise:
s101, designing and building an electrolytic tank, wherein the electrolytic tank has the functions of adjusting current, voltage and temperature parameters, and an anode and a cathode are arranged in the electrolytic tank and are connected with an external power supply through wires;
S102, mixing sea salt and purified water according to a proportion to form an electrolyte solution, and injecting the electrolyte solution into an electrolytic tank;
In the embodiment, the adding mass ratio of sea salt to purified water is 1:10;
s103, switching on a power supply;
S104, current flows between the anode and the cathode to trigger electrolytic reaction, oxidation reaction occurs on the anode, reduction reaction occurs on the cathode to generate sodium hydroxide solution, chlorine and hydrogen;
In this embodiment, the chemical reaction equation is as follows:
2NaCl2+2H2O＝2NaOH+Cl2↑+H2↑
s105, separating and purifying sodium hydroxide solution, chlorine and hydrogen generated by electrolysis;
S106, reacting the purified chlorine gas with sodium chlorite solution to generate food-grade chlorine dioxide gas and sodium chloride solution;
In this embodiment, the chemical reaction equation is as follows:
Cl2+2NaClO2＝2ClO2↑+2NaCl
the addition ratio of the chlorine to the sodium chlorite solution needs to meet the completion of the chemical reaction;
s107, separating and purifying the food-grade chlorine dioxide gas and sodium chloride solution generated by the reaction, and supplementing sea salt components in the step S102 by the purified sodium chloride solution;
s108, introducing purified food-grade chlorine dioxide gas into water, mixing with the water, and detecting the PH value;
in the embodiment, the addition ratio of the food grade chlorine dioxide gas to the water is 1:1000;
And S109, introducing purified sodium hydroxide solution or water into the reactor to adjust the pH value to 8.5-9.5 according to the detected pH value.
Specifically, the preparation steps of the food-grade chlorine dioxide further comprise:
s110, reacting the sodium hydroxide solution purified in the step S105 and the food-grade chlorine dioxide gas purified in the step S107 with hydrogen peroxide solution under the condition of surplus to generate sodium chlorite solution, oxygen and water;
In this embodiment, the chemical reaction equation is as follows:
2ClO2+H2O2+2NaOH＝2NaClO2+O2↑+2H2O
The addition ratio of the sodium hydroxide solution, the food-grade chlorine dioxide gas and the hydrogen peroxide solution needs to meet the completion of the chemical reaction;
And S111, separating and purifying the sodium chlorite solution, oxygen and water generated by the reaction, wherein the purified sodium chlorite solution supplements the sodium chlorite solution in the step S106, and the purified water supplements the purified water in the step S102.
Specifically, the preparation steps of the food-grade chlorine dioxide further comprise:
s112, reacting the hydrogen purified in the step S105 and the oxygen purified in the step S111 with ethyl anthraquinone to generate hydrogen peroxide solution;
In this embodiment, the chemical reaction equation is as follows:
In this embodiment, the addition ratio of hydrogen, oxygen and ethyl anthraquinone needs to satisfy the completion of the chemical reaction;
And S113, separating and purifying the hydrogen peroxide solution generated by the reaction, and supplementing the hydrogen peroxide solution in the step S110 with the purified hydrogen peroxide solution.
Specifically, the preparation method of sericin comprises the following steps:
s201, preparing multiplied cocoons;
s202, cutting cocoons into small pieces through scissors;
s203, cleaning small cocoons with deionized water to remove surface impurities;
S204, placing the cleaned small cocoons in a heating container, adding sodium hydroxide solution, heating and stirring to dissolve the cocoons in the sodium hydroxide solution, and filtering to remove insoluble impurities;
in the embodiment, the adding proportion of the silkworm cocoons and the sodium hydroxide solution is that the sodium hydroxide solution is used for immersing the silkworm cocoons;
The heating temperature is 80-90 ℃;
s205, centrifuging the filtered mixture to separate sericin solution;
S206, freeze-drying the sericin solution to remove part of water.
Specifically, the preparation method of the oxalis extract comprises the following steps:
s301, picking fresh whole herb of oxalis;
s302, cleaning and drying picked fresh whole herb of oxalis, and removing surface impurities;
s303, placing the dried oxalis mother grass in a crusher for crushing, and pulverizing into powder;
s304, soaking and steaming the oxalis powder in distilled water twice, filtering out filtrate, combining the filtrates, and cooling to room temperature;
In the embodiment, the addition ratio of the oxalis powder to distilled water is 1:10;
soaking time is 12 hours, cooking temperature is 80 ℃, and cooking time is 1.5 hours;
Specifically, the preparation method of the fructo-oligosaccharide comprises the following steps:
S401, placing sucrose in water and mixing with the water to generate a sucrose solution;
in the embodiment, the adding ratio of sucrose to water is 1:10;
S402, inoculating Aspergillus niger into a sucrose solution for fermentation, wherein FTS enzyme generated in the fermentation process catalyzes sucrose to perform a transfer reaction, so that fructo-oligosaccharide is generated;
s403, filtering the produced fructo-oligosaccharide to separate the fructo-oligosaccharide;
S404, adsorbing pigment of fermentation liquor adhered on fructo-oligosaccharide through an activated carbon column;
S405, adsorbing the salt of the fermentation broth adhered to the fructo-oligosaccharide by ion exchange resin;
S406, removing the adhered moisture on the fructo-oligosaccharide by freeze drying.
The substances participating in the reaction are recycled continuously under the surplus condition, and if the reaction substances are not sufficiently recycled in the process, the substances can be directly supplemented from the outside.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
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