CN107647385A - A kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets - Google Patents
A kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets Download PDFInfo
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- CN107647385A CN107647385A CN201710643592.2A CN201710643592A CN107647385A CN 107647385 A CN107647385 A CN 107647385A CN 201710643592 A CN201710643592 A CN 201710643592A CN 107647385 A CN107647385 A CN 107647385A
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- fishbone dust
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
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Abstract
The invention discloses a kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets, including rough fishbone dust, fishbone dust fermentation, the homogenate of stepwise discretization deep-sea fish, isoelectric precipitation, second ultrafiltration, freeze-drying and tabletting.Have the beneficial effect that：The composition of marine protein matter amino acid or the sequence of amino acid are all very different with terrestrial life albumen, and many different active peptide segments are can release with marine protein enzyme enzymolysis marine product.Chewable tablets free calcium, vitamin and the small protein content being prepared are high, and also containing needed by human body elements such as phosphorus, magnesium, iron, zinc.Human body is good to its calcium absorption effect, and long-term use of calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt content, cortex of bone area, femur energy absorption etc. are in significantly increase trend.
Description
Technical field
The present invention relates to health products preparation field, the preparation side of specifically a kind of fishbone dust and protein peptide mixture chewable tablets
Method.
Background technology
China is aquatic products big producer, and the yield of annual China fresh-water fishes and ocean fish is in the trend that grows steadily.In recent years,
China's fish processing industry is quickly grown, and existing oneself forms the classes such as refrigerated products, salted fish product, feeding fish meal, minced fillet and its product
The product of type.Surimi product has the characteristics that high protein, low fat, numerous in variety, storage endurance are liked by consumers in general deeply because of it
Love, turn into the Main Means of fish processing, but its flesh collecting rate typically only 25 ~ 30%.Adopt and contain what is enriched in the remaining fish body of meat
The mineral matters such as calcium, phosphorus, it is a kind of good natural calcium source.
Naturally occurring Marine Bioactive Peptides due in organism content it is low, and extract difficult, it is difficult to realize a large amount of
Needed for production supply, chemical artificial synthetic polypeptide is costly.So sight is more invested exploitation proteolysis production by people
Come in this approach of thing acquisition active peptides.Because the environment and terrestrial life of marine organisms existence are entirely different, such as high pressure, low
The extreme environment such as temperature, high temperature and high salt, in order to adapt to these extreme marine biological environments, marine protein matter no matter amino
The composition or the sequence of amino acid of acid are all very different with terrestrial life albumen, meanwhile, marine protein resource without
By being all far longer than land protein resource in type and quantity, and do not developed well.Miscellaneous ocean egg
In casamino acid sequence, potential many amino acid sequences with bioactivity, with special protease hydrolytic, just discharge
Active peptide fragment.Marine protein resource is the important of the important albumen group food of the 21 century mankind and bioactive substance
Source.The current marine protein total resources in China comes out at the top in countries in the world, but the protide aquatic products that China is current
In product, in addition to a part is directly edible, major part is made food by simple process technology and enters market, and process technology falls
Afterwards, product structure is single, and value-added content of product is low so that the competitiveness of product in the international market is poor.Therefore, China is badly in need of
Marine protein resource is optimized and utilized and high-valued processing.
The content of the invention
It is an object of the invention to provide a kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets, and human body is to system
The calcium absorption effect of standby obtained chewable tablets is good, long-term use of calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt
Content, cortex of bone area, femur energy absorption etc. are in significantly increase trend.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：A kind of fishbone dust and protein peptides are mixed
The preparation method of compound chewable tablets, including rough fishbone dust, fishbone dust fermentation, the homogenate of stepwise discretization deep-sea fish, isoelectric precipitation,
Second ultrafiltration, freeze-drying and tabletting.Fishbone dust fermentation step is：Fishbone dust mixes with pure water, glucose and potato extract solution
Close and lactobacillus-fermented culture medium is made, high-temperature sterilization, inoculating lactic acid bacterium after cooling, ferment 3 ~ 7d under the conditions of 28 ~ 32 DEG C, concentration
Drying and grinding obtains fishbone dust of fermenting.Fishbone dust is 14 ~ 25 with pure water, glucose and potato extract solution mass ratio:60~75:2
~4:3~6；Lactic acid bacteria is plant embryo lactobacillus.Lactic acid, acetic acid, pyruvic acid, butanedioic acid, fumaric acid etc. are produced in fermentation process
The metabolin such as organic acid and free amino acid, polysaccharide, enzyme, the calcium in fish-bone is converted into free state from reference state.In zymotic fluid
Free calcium ions content is far above milk, and also high containing the needed by human body elements such as phosphorus, magnesium, iron, zinc, nutritive value.
Rough fishbone dust step is：30 ~ 40wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 25 ~ 35min, fish
The volume ratio of bone and sodium carbonate liquor is 1:2.5 ~ 4.5, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure
0.14 ~ 0.18MPa of power, 4 ~ 7min of boiling, drying and crushing obtain fishbone dust.
Stepwise discretization deep-sea fish refining step is that 20 ~ 30 times of volume pure water homogenate are added in deep-sea fish minced fillet, adds 5
The undaria pinnitafida protease of ~ 8wt ‰ and the bioactive peptides of 0.2 ~ 0.7wt ‰, in 25 ~ 35 DEG C, under the conditions of pH7.0 ~ 9.0 digest 30 ~
40min, regulation pH are 6.0 ~ 7.0, add 1 ~ 3wt ﹪ flavor proteases, digest 15 ~ 25min in 52 ~ 60 DEG C, obtain enzymolysis liquid；
The amino acid sequence of the bioactive peptide is SCASRCKVSFRCAVSFCICKCFRC.Above-mentioned bioactive peptide can pass through Van der Waals force
Change the tertiary structure of undaria pinnitafida protease with electrostatic interaction, the enzymolysis vigor of undaria pinnitafida protease is improved significantly.
The composition of marine protein matter amino acid or the sequence of amino acid are all very different with terrestrial life albumen, use
Marine protein enzyme enzymolysis marine product can release many different active peptide segments.Undaria pinnitafida protease is first used, on this basis
Continue to digest using flavor protease, can obtain protein peptides, above-mentioned protein peptides can promote body in the case where being irradiated without ultraviolet
The synthesis of interior vitamin D.Do not have at present and obtain the report of above-mentioned protein peptides using other protease hydrolyzeds.Using marine protein enzyme
Digest, have significant creative with land proteinase synergy.
For isoelectric precipitation step for enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.5 ~ 4.8, and centrifuging and taking is sunk after standing
Form sediment.Under above-mentioned pH environment, protein peptides are in electroneutral, are slightly soluble in water, most of to be separated with precipitation form from the aqueous solution.
Second ultrafiltration and nano-filtration step are first to use 30 ~ 40kD of retention relative molecular mass film ultrafiltration, then using retention
4 ~ 8kD of relative molecular mass film ultrafiltration, filtered solution is finally subjected to nanofiltration desalination.In order to prevent Pore Blocking or membrane damage,
Ultrafiltration is first carried out using big porous ultrafiltration membrane, then using small porous ultrafiltration membrane ultrafiltration, effectively removes the impurity that molecular weight is more than 4 ~ 8kD.
Nanofiltration can be more thoroughly remove solution in inorganic salts and molecular weight be less than 200Da impurity, protein peptides are further carried out pure
Change.
It is freeze-dried in step and adds 20 ~ 35wt% polyethylene Topiramate Los oxazolidinone as lyophilisation additives, is freeze-dried and obtains egg
White Gly-His-Lys.Protein peptides need to be maintained at the stabilization of property during storage containing suitable residual moisture, and excessive drying will
Make protein molecule surface hydrogen bond and polar group exposure and be denatured.In freezing dry process, polyethylene Topiramate Los oxazolidinone is added,
Can the albumen stretching, extension of protected protein peptide, quaternary structure, so as to being protected to albumen peptide activity.Above-mentioned protein peptides can be without ultraviolet
In the case that line irradiates, promote the synthesis of internal vitamin D, and then improve human body to absorption rate calcareous in fishbone dust,
But the specific mechanism of action is still not clear.
Tableting step be by 30 ~ 50 parts of fishbone dusts of fermenting, 16 ~ 27 parts of protein peptide powders, 0.03 ~ 0.09 part of magnesium stearate and
0.5 ~ 1.6 part of silica mixes, compressing in feeding tablet press machine, obtains fishbone dust and protein peptide mixture chewable tablets.On
It is high to state chewable tablets free calcium, vitamin and small protein content, and also containing the needed by human body such as phosphorus, magnesium, iron, zinc member
Element.Human body is good to its calcium absorption effect, long-term use of calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt content,
Cortex of bone area, femur energy absorption etc. are in significantly increase trend.
Compared with prior art, the advantage of the invention is that：
1. the composition of marine protein matter amino acid or the sequence of amino acid all have very big with terrestrial life albumen
Difference, many different active peptide segments are can release with marine protein enzyme enzymolysis marine product.Undaria pinnitafida protease is first used, herein
On the basis of continue to digest using flavor protease, can obtain protein peptides, above-mentioned protein peptides can in the case where being irradiated without ultraviolet,
Promote the synthesis of internal vitamin D.
2. amino acid sequence is added when undaria pinnitafida protease hydrolyzed deep-sea fish is homogenized is
SCASRCKVSFRCAVSFCICKCFRC bioactive peptide, above-mentioned bioactive peptide can be changed by Van der Waals force and electrostatic interaction
The tertiary structure of undaria pinnitafida protease, the enzymolysis vigor of undaria pinnitafida protease is set to be improved significantly.
3. the chewable tablets free calcium being prepared, vitamin and small protein content are high, and also containing phosphorus, magnesium,
The needed by human body element such as iron, zinc.Human body is good to its calcium absorption effect, and long-term use of calcium apparent absorptivity, calcium Retention, bone are close
Degree, bone mineral salt content, cortex of bone area, femur energy absorption etc. are in significantly increase trend.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets, including rough fishbone dust, fishbone dust fermentation, substep enzyme
Solve deep-sea fish homogenate, isoelectric precipitation, second ultrafiltration, freeze-drying and tabletting.Fishbone dust fermentation step is：Fishbone dust with it is pure
Water, glucose and potato extract solution are mixed to prepare lactobacillus-fermented culture medium, high-temperature sterilization, inoculating lactic acid bacterium after cooling, in
Ferment 3 ~ 7d under the conditions of 28 ~ 32 DEG C, is concentrated and dried grinding and obtains fishbone dust of fermenting.Fishbone dust and pure water, glucose and potato
Extract solution mass ratio is 14 ~ 25:60~75:2~4:3~6；Lactic acid bacteria is plant embryo lactobacillus.In fermentation process produce lactic acid,
The metabolin such as the organic acids such as acetic acid, pyruvic acid, butanedioic acid, fumaric acid and free amino acid, polysaccharide, enzyme, by the calcium in fish-bone from
Reference state is converted into free state.The calcium ion content that dissociates in zymotic fluid is far above milk, and also containing phosphorus, magnesium, iron, zinc et al.
Element needed for body, nutritive value are high.
Rough fishbone dust step is：30 ~ 40wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 25 ~ 35min, fish
The volume ratio of bone and sodium carbonate liquor is 1:2.5 ~ 4.5, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure
0.14 ~ 0.18MPa of power, 4 ~ 7min of boiling, drying and crushing obtain fishbone dust.
Stepwise discretization deep-sea fish refining step is that 20 ~ 30 times of volume pure water homogenate are added in deep-sea fish minced fillet, adds 5
The undaria pinnitafida protease of ~ 8wt ‰ and the bioactive peptides of 0.2 ~ 0.7wt ‰, in 25 ~ 35 DEG C, under the conditions of pH7.0 ~ 9.0 digest 30 ~
40min, regulation pH are 6.0 ~ 7.0, add 1 ~ 3wt ﹪ flavor proteases, digest 15 ~ 25min in 52 ~ 60 DEG C, obtain enzymolysis liquid；
The amino acid sequence of bioactive peptide is SCASRCKVSFRCAVSFCICKCFRC.Marine protein matter no matter the composition of amino acid
Or the sequence of amino acid is all very different with terrestrial life albumen, can release perhaps with marine protein enzyme enzymolysis marine product
More different active peptide segments.Undaria pinnitafida protease is first used, continues to digest using flavor protease on this basis, can obtain egg
White peptide, above-mentioned protein peptides in the case where being irradiated without ultraviolet, can promote the synthesis of internal vitamin D.Do not have using it at present
His protease hydrolyzed obtains the report of above-mentioned protein peptides.Digested, had notable using marine protein enzyme and land proteinase synergy
Creativeness.
For isoelectric precipitation step for enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.5 ~ 4.8, and centrifuging and taking is sunk after standing
Form sediment.Under above-mentioned pH environment, protein peptides are in electroneutral, are slightly soluble in water, most of to be separated with precipitation form from the aqueous solution.
Above-mentioned bioactive peptide can change the tertiary structure of undaria pinnitafida protease by Van der Waals force and electrostatic interaction, make undaria pinnitafida albumen
The enzymolysis vigor of enzyme is improved significantly.
Second ultrafiltration and nano-filtration step are first to use 30 ~ 40kD of retention relative molecular mass film ultrafiltration, then using retention
4 ~ 8kD of relative molecular mass film ultrafiltration, filtered solution is finally subjected to nanofiltration desalination.In order to prevent Pore Blocking or membrane damage,
Ultrafiltration is first carried out using big porous ultrafiltration membrane, then using small porous ultrafiltration membrane ultrafiltration, effectively removes the impurity that molecular weight is more than 4 ~ 8kD.
Nanofiltration can be more thoroughly remove solution in inorganic salts and molecular weight be less than 200Da impurity, protein peptides are further carried out pure
Change.
It is freeze-dried in step and adds 20 ~ 35wt% polyethylene Topiramate Los oxazolidinone as lyophilisation additives, is freeze-dried and obtains egg
White Gly-His-Lys.Protein peptides need to be maintained at the stabilization of property during storage containing suitable residual moisture, and excessive drying will
Make protein molecule surface hydrogen bond and polar group exposure and be denatured.In freezing dry process, polyethylene Topiramate Los oxazolidinone is added,
Can the albumen stretching, extension of protected protein peptide, quaternary structure, so as to being protected to albumen peptide activity.Above-mentioned protein peptides can be without ultraviolet
In the case that line irradiates, promote the synthesis of internal vitamin D, and then improve human body to absorption rate calcareous in fishbone dust,
But the specific mechanism of action is still not clear.
Tableting step be by 30 ~ 50 parts of fishbone dusts of fermenting, 16 ~ 27 parts of protein peptide powders, 0.03 ~ 0.09 part of magnesium stearate and
0.5 ~ 1.6 part of silica mixes, compressing in feeding tablet press machine, obtains fishbone dust and protein peptide mixture chewable tablets.On
It is high to state chewable tablets free calcium, vitamin and small protein content, and also containing the needed by human body such as phosphorus, magnesium, iron, zinc member
Element.Human body is good to its calcium absorption effect, long-term use of calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt content,
Cortex of bone area, femur energy absorption etc. are in significantly increase trend.
Embodiment 2：
A kind of fishbone dust and the most preferably preparation method of protein peptide mixture chewable tablets, comprise the following steps：
1）Rough fishbone dust：34wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 32min, fish-bone and sodium carbonate liquor
Volume ratio be 1:3.6, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure 0.15MPa, boiling
5min, drying and crushing obtain fishbone dust；
2）Fishbone dust is fermented：Fishbone dust is mixed to prepare lactobacillus-fermented culture medium with pure water, glucose and potato extract solution, high
Temperature sterilizing, inoculating lactic acid bacterium after cooling, ferment 6d under the conditions of 30 DEG C, is concentrated and dried grinding and obtains fishbone dust of fermenting.Fishbone dust
It is 20 with pure water, glucose and potato extract solution mass ratio:65:3: 6；Lactic acid bacteria is plant embryo lactobacillus；
3）Stepwise discretization deep-sea fish is homogenized：24 times of volume pure water homogenate are added in deep-sea fish minced fillet, add the undaria pinnitafidas of 7wt ‰
Protease and the bioactive peptides of 0.6wt ‰, in 28 DEG C, 35min is digested under the conditions of pH8.0, regulation pH is 6.6, adds 2wt ﹪ flavors
Protease, 19min is digested in 55 DEG C, obtains enzymolysis liquid；The amino acid sequence of bioactive peptide is
SCASRCKVSFRCAVSFCICKCFRC；
4）Isoelectric precipitation：Enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.6, and centrifuging and taking precipitates after standing；
5）Second ultrafiltration and nanofiltration：First using retention relative molecular mass 35kD film ultrafiltration, then using retention average molecular matter
6kD film ultrafiltration is measured, filtered solution is finally subjected to nanofiltration desalination；
6）Freeze-drying：32wt% polyethylene Topiramate Los oxazolidinone is added in filtered solution after nanofiltration desalination as lyophilisation additives, it is cold
Jelly is dried to obtain protein peptide powder；
7）Tableting step is to mix 40 parts of ferment fishbone dust, 25 parts of protein peptide powders, 0.07 part of magnesium stearate and 1.1 parts of silica
Close, it is compressing in feeding tablet press machine, obtain fishbone dust and protein peptide mixture chewable tablets.Above-mentioned chewable tablets free calcium, dimension life
Element and small protein content are high, and also containing needed by human body elements such as phosphorus, magnesium, iron, zinc.Human body is imitated to its calcium absorption
Fruit is good, and long-term use of calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt content, cortex of bone area, femur energy are inhaled
Receipts etc. are in significantly increase trend.
Embodiment 3：
A kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets, comprise the following steps：
1）Rough fishbone dust：30wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 30min, fish-bone and sodium carbonate liquor
Volume ratio be 1:3.5, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure 0.13MPa, boiling
3min, drying and crushing obtain fishbone dust；
2）Fishbone dust is fermented：Fishbone dust is mixed to prepare lactobacillus-fermented culture medium with pure water, glucose and potato extract solution, high
Temperature sterilizing, inoculating lactic acid bacterium after cooling, ferment 7d under the conditions of 301 DEG C, is concentrated and dried grinding and obtains fishbone dust of fermenting.Fishbone dust
It is 22 with pure water, glucose and potato extract solution mass ratio:70:3: 6；Lactic acid bacteria is plant embryo lactobacillus；
3）Stepwise discretization deep-sea fish is homogenized：22 times of volume pure water homogenate are added in deep-sea fish minced fillet, add the undaria pinnitafidas of 7wt ‰
Protease and the bioactive peptides of 0.6wt ‰, in 28 DEG C, 35min is digested under the conditions of pH8.0, regulation pH is 6.6, adds 2wt ﹪ flavors
Protease, 19min is digested in 55 DEG C, obtains enzymolysis liquid；The amino acid sequence of bioactive peptide is
SCASRCKVSFRCAVSFCICKCFRC；
4）Isoelectric precipitation：Enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.6, and centrifuging and taking precipitates after standing；
5）Second ultrafiltration and nanofiltration：First using retention relative molecular mass 35kD film ultrafiltration, then using retention average molecular matter
6kD film ultrafiltration is measured, filtered solution is finally subjected to nanofiltration desalination；
6）Freeze-drying：32wt% polyethylene Topiramate Los oxazolidinone is added in filtered solution after nanofiltration desalination as lyophilisation additives, it is cold
Jelly is dried to obtain protein peptide powder；
7）Tableting step is to mix 40 parts of ferment fishbone dust, 25 parts of protein peptide powders, 0.07 part of magnesium stearate and 1.1 parts of silica
Close, it is compressing in feeding tablet press machine, obtain fishbone dust and protein peptide mixture chewable tablets.Above-mentioned chewable tablets free calcium, dimension life
Element and small protein content are high, and also containing needed by human body elements such as phosphorus, magnesium, iron, zinc.Human body is imitated to its calcium absorption
Fruit is good, and long-term use of calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt content, cortex of bone area, femur energy are inhaled
Receipts etc. are in significantly increase trend.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Pujiang County is safe such as food science and technology Co., Ltd
<120>A kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 24
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Val Ser Phe Arg Cys Ala Val Ser Phe
1 5 10 15
Cys Ile Cys Lys Cys Phe Arg Cys
20
Claims (8)
1. a kind of fishbone dust and the preparation method of protein peptide mixture chewable tablets, including rough fishbone dust, fishbone dust fermentation, substep
Digest deep-sea fish homogenate, isoelectric precipitation, second ultrafiltration, freeze-drying and tabletting, it is characterised in that：Described fishbone dust fermentation
Step is：Fishbone dust is mixed to prepare lactobacillus-fermented culture medium with pure water, glucose and potato extract solution, and high-temperature sterilization is cold
But inoculating lactic acid bacterium after, ferment 3 ~ 7d under the conditions of 28 ~ 32 DEG C, is concentrated and dried grinding and obtains fishbone dust of fermenting.
2. a kind of fishbone dust according to claim 1 and the preparation method of protein peptide mixture chewable tablets, it is characterised in that：
Described fishbone dust is 14 ~ 25 with pure water, glucose and potato extract solution mass ratio:60~75:2~4:3~6；The lactic acid bacteria
For plant embryo lactobacillus.
3. a kind of fishbone dust according to claim 1 and the preparation method of protein peptide mixture chewable tablets, it is characterised in that：
Described rough fishbone dust step is：30 ~ 40wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 25 ~ 35min, fish-bone
Volume ratio with sodium carbonate liquor is 1:2.5 ~ 4.5, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure
0.14 ~ 0.18MPa, 4 ~ 7min of boiling, drying and crushing obtain fishbone dust.
4. a kind of fishbone dust according to claim 1 and the preparation method of protein peptide mixture chewable tablets, it is characterised in that：
Described stepwise discretization deep-sea fish refining step is that the homogenate of 20 ~ 30 times of volume pure water is added in deep-sea fish minced fillet, add 5 ~
The undaria pinnitafida protease of 8wt ‰ and the bioactive peptides of 0.2 ~ 0.7wt ‰, in 25 ~ 35 DEG C, 30 ~ 40min is digested under the conditions of pH7.0 ~ 9.0,
It is 6.0 ~ 7.0 to adjust pH, adds 1 ~ 3wt ﹪ flavor proteases, digests 15 ~ 25min in 52 ~ 60 DEG C, obtains enzymolysis liquid；The work
The amino acid sequence of property small peptide is SCASRCKVSFRCAVSFCICKCFRC.
5. a kind of fishbone dust according to claim 1 and the preparation method of protein peptide mixture chewable tablets, it is characterised in that：
For described isoelectric precipitation step for enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.5 ~ 4.8, and centrifuging and taking precipitates after standing.
6. a kind of fishbone dust according to claim 1 and the preparation method of protein peptide mixture chewable tablets, it is characterised in that：
Described second ultrafiltration and nano-filtration step are first to use 30 ~ 40kD of retention relative molecular mass film ultrafiltration, then using retention phase
Film ultrafiltration to 4 ~ 8kD of molecular mass, filtered solution is finally subjected to nanofiltration desalination.
7. a kind of fishbone dust according to claim 1 and the preparation method of protein peptide mixture chewable tablets, it is characterised in that：
20 ~ 35wt% polyethylene Topiramate Los oxazolidinone is added in described freeze-drying step and obtains albumen as lyophilisation additives, freeze-drying
Gly-His-Lys.
8. a kind of fishbone dust according to claim 1 and the preparation method of protein peptide mixture chewable tablets, it is characterised in that：
Described tableting step be by 30 ~ 50 parts of ferment fishbone dusts, 16 ~ 27 parts of protein peptide powders, 0.03 ~ 0.09 part of magnesium stearate and 0.5 ~
1.6 parts of silica mix, compressing in feeding tablet press machine, obtain fishbone dust and protein peptide mixture chewable tablets.
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| CN111820281A (en) * | 2020-07-27 | 2020-10-27 | 上海交通大学 | Sustained-release nutritional VC lutein peptide chewable tablet and preparation method thereof |
| CN111990651A (en) * | 2020-08-25 | 2020-11-27 | 大连工业大学 | A kind of fish meal lozenge containing polysaccharide and preparation method thereof |
| CN112205592A (en) * | 2020-10-29 | 2021-01-12 | 山东承锦海洋生物科技有限公司 | Preparation method of sturgeon freeze-dried powder and sturgeon freeze-dried powder |
| CN112293728A (en) * | 2020-07-13 | 2021-02-02 | 丽江美之源食品有限公司 | Fructus phyllanthi probiotic buccal tablet |
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| CN106616528A (en) * | 2016-11-30 | 2017-05-10 | 山东好当家海洋发展股份有限公司 | Preparation method for fermentation type fish bone powder chewable tablets |
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| CN102406176A (en) * | 2011-10-08 | 2012-04-11 | 山东荣信水产食品集团股份有限公司 | Fishbone small-molecule polypeptide chelated calcium and preparation method thereof |
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| CN112293728A (en) * | 2020-07-13 | 2021-02-02 | 丽江美之源食品有限公司 | Fructus phyllanthi probiotic buccal tablet |
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Application publication date: 20180202 |