CN117898969A

CN117898969A - Ceramide composition with effects of repairing and thickening skin barrier and preparation method thereof

Info

Publication number: CN117898969A
Application number: CN202410070944.XA
Authority: CN
Inventors: 郑宇强; 王婷婷
Original assignee: Ningbo Botong Weida Biomedical Co ltd
Current assignee: Ningbo Botong Weida Biomedical Co ltd
Priority date: 2024-01-17
Filing date: 2024-01-17
Publication date: 2024-04-19

Abstract

The invention belongs to the field of cosmetics, and in particular relates to a ceramide composition with the functions of repairing and thickening skin barriers and a preparation method thereof. The ceramide composition comprises a phase A, a phase B and a phase C; wherein: phase a comprises glycerol, hydrogenated lecithin, and purified water; phase B includes caprylic/capric triglyceride, octyldodecanol, behenyl alcohol, ceramide NP, ceramide NS/ceramide NG, and ceramide AP; the phase C comprises squalane, butter fruit, polyglycerol-3 methyl glucose distearate, bisabolol, herba Portulacae extract, sodium hyaluronate, beta-dextran, and butanediol. The ceramide composition supplements the stratum corneum from multiple dimensions, and free fatty acid and shea butter and squalane which are subjected to cholesterol decomposition can cooperate with ceramide to form a human-like simulated stratum corneum, and remodel the stratum corneum accordingly; and the ceramide composition has the property of repairing skin barrier and thickening sebum membrane.

Description

Ceramide composition with effects of repairing and thickening skin barrier and preparation method thereof

Technical Field

The invention belongs to the field of cosmetics, and in particular relates to a ceramide composition with the functions of repairing and thickening skin barriers and a preparation method thereof.

Background

In modern life, with the increasing serious atmospheric pollution, the skin is used as the first line of defense of the body, and is subjected to continuous external invasion, excessive water loss is easily caused after the skin is damaged, so that the invasion of disease microorganisms is caused, and the molecular transport channels are disturbed, the aging is accelerated, and finally the skin is dry, dull and flawed. While the skin barrier is mainly derived from the outermost layer of the epidermis, the stratum corneum, the "brick wall structure" consisting of keratinocytes and intercellular lipids is the structural basis of the skin barrier currently accepted by the skin care community. Wherein the ceramide is one of the most core active substances in intercellular lipids, and accounts for more than 40 percent. Ceramide is composed of long-chain sphingosine base and fatty acid, and is involved in the processes of signal transduction, regulation of skin immunity, cell cycle, differentiation, apoptosis and the like.

At present, the common maintenance effect of ceramide maintenance products is low, and the main reasons are as follows:

1. the addition concentration is low, and the general addition is trace addition (the addition amount of the cosmetic raw material is less than or equal to 0.1 percent and is trace addition);

2. the additive types are single, and the synergistic effect is not generated. Intercellular lipids, besides 40% of ceramide, 30% of free fatty acid, 25% of cholesterol, etc., cannot be expected by increasing ceramide alone, and TWEL repair efficacy.

Therefore, the technical scheme of the invention is provided based on the above.

Disclosure of Invention

In order to solve the problems of the prior art, the present invention provides a ceramide composition with repairing and thickening skin barrier, which comprises a phase A, a phase B and a phase C; wherein:

the phase A comprises the following raw materials in parts by weight: 1 to 10 parts of glycerin, 0.5 to 5 parts of hydrogenated lecithin and 45 to 75 parts of purified water;

the phase B comprises the following raw materials in parts by weight: 0.1 to 10 parts of caprylic/capric triglyceride, 0.01 to 2 parts of octyl dodecanol, 0.01 to 2 parts of behenyl alcohol, 0.5 to 5 parts of ceramide NP, 0.01 to 2 parts of ceramide NS/ceramide NG and 0.01 to 2 parts of ceramide AP;

The phase C comprises the following raw materials in parts by weight: 1 to 5 parts of squalane, 1 to 5 parts of butter fruit tree fruit fat, 1 to 5 parts of polyglycerol-3 methyl glucose distearate, 0.5 to 3 parts of bisabolol, 0.5 to 3 parts of purslane extract, 0.001 to 0.1 part of sodium hyaluronate, 0.5 to 5 parts of beta-glucan and 1 to 10 parts of butanediol.

Preferably, the phase A comprises the following raw materials in parts by weight: 5.5 parts of glycerol, 2.5 parts of hydrogenated lecithin and 60 parts of purified water.

Preferably, the phase B comprises the following raw materials in parts by weight: 5 parts of caprylic/capric triglyceride, 1 part of octyl dodecanol, 1 part of behenyl alcohol, 2.5 parts of ceramide NP, 1 part of ceramide NS/ceramide NG and 1 part of ceramide AP.

Preferably, the C phase comprises the following raw materials in parts by weight: 3 parts of squalane, 3 parts of butter fruit tree fruit fat, 3 parts of polyglycerol-3-methyl glucose distearate, 1.5 parts of bisabolol, 1.5 parts of purslane extract, 0.05 part of sodium hyaluronate, 2.5 parts of beta-glucan and 5.5 parts of butanediol.

Based on the same technical concept, still another aspect of the present invention is to provide a preparation method of a ceramide composition having a repairing, thickening skin barrier, the preparation method comprising the steps of:

(1) Mixing glycerol, hydrogenated lecithin and purified water, and uniformly stirring to obtain a phase A;

(2) Mixing caprylic/capric triglyceride, octyl dodecanol, behenyl alcohol, ceramide NP, ceramide NS/ceramide NG and ceramide AP, and uniformly stirring to obtain a phase B;

(3) Mixing the phase A and the phase B again to obtain a mixed phase;

(4) Adding squalane, shea butter, polyglycerol-3 methyl glucose distearate, bisabolol, herba Portulacae extract, sodium hyaluronate, beta-glucan and butanediol into the mixed phase, stirring, and aging to obtain the ceramide composition with repairing and skin thickening effects.

Preferably, in the step (1), glycerin, hydrogenated lecithin and purified water are mixed, heated to 60-65 ℃ and kept for 0.5-1 h until being completely dissolved, and then stirred uniformly to obtain phase A.

Preferably, in the step (2), caprylic/capric triglyceride, octyldodecanol, behenyl alcohol, ceramide NP, ceramide NS/ceramide NG and ceramide AP are mixed, heated to 75-85 ℃, kept for 0.5-1 h until being completely dissolved, and then stirred uniformly to obtain phase B.

Preferably, in the step (4), under the stirring condition, controlling the temperature of a system to be 40-45 ℃, adding squalane, butter tree fruit fat, polyglycerol-3 methyl glucose distearate, bisabolol, purslane extract, sodium hyaluronate, beta-glucan and butanediol into the mixed phase, stirring for 0.5-1 h, and then aging for 15-20 h at room temperature to obtain the ceramide composition with the skin repairing and thickening barrier.

The beneficial effects of the invention are as follows:

the ceramide composition with the functions of repairing and thickening skin barriers, which is required for supplementing the stratum corneum from multiple dimensions, has free fatty acid and Shea butter and squalane which are subjected to cholesterol decomposition, can cooperate with ceramide to form a human-like simulated stratum corneum, and remodels the stratum corneum; in addition, the high-concentration ceramide added by the invention has stability in a system, is not easy to separate out, can be obviously reserved in a stratum corneum, has a repairing effect, and simultaneously eliminates the sticky and thick skin feel of the traditional ceramide composition; finally, experiments show that the ceramide composition has the performance of repairing skin barrier and thickening sebum membrane.

The preparation method disclosed by the invention is convenient to operate, does not need complex equipment, is environment-friendly, and has extremely high popularization value.

Drawings

In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a graph showing a comparison of the biomimetic transdermal effects of simple ceramide oil and the composition (liposome) obtained in example 1 of the present invention.

FIG. 2 is a graph showing the results of ceramide content in skin.

Fig. 3 is a graph of skin TEWL mean change data.

Fig. 4 is a graph of skin heme content variation data.

Fig. 5 is a graph of red area ratio change data.

FIG. 6 is a partial red plot of VISIA for subject No. 13.

Fig. 7 is a partial red plot of VISIA for subject No. 16.

Fig. 8 is a graph of skin horny layer moisture content variation data.

Fig. 9 is a graph of skin layer thickness variation data.

Fig. 10 is a chart of variation in epidermal layer thickness under a sonogram for subject No. 18.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, based on the examples herein, which are within the scope of the invention as defined by the claims, will be within the scope of the invention as defined by the claims.

Example 1

The present example provides a method of preparing a ceramide composition having a repaired, thickened skin barrier, the method comprising the steps of:

(1) Mixing 10g of glycerol, 5g of hydrogenated lecithin and 450g of purified water, stirring and heating to 60 ℃, keeping for 0.5h until the mixture is completely dissolved, and uniformly stirring to obtain a phase A;

(2) Mixing 1g of caprylic/capric triglyceride, 0.1g of octyl dodecanol, 0.1g of behenyl alcohol, 5g of ceramide NP, 0.1g of ceramide NS/ceramide NG and 0.1g of ceramide AP, heating to 75 ℃, keeping for 0.5h till complete dissolution, and stirring uniformly to obtain a phase B;

(3) Mixing the phase A and the phase B again to obtain a mixed phase;

(4) Under the stirring condition, controlling the temperature of a system to be 40 ℃, adding 10g of squalane, 10g of shea butter, 10g of polyglycerol-3 methyl glucose distearate, 5g of bisabolol, 5g of purslane extract, 0.01g of sodium hyaluronate, 5g of beta-glucan and 10g of butanediol into the mixed phase, stirring for 0.5h, and then aging for 15h at room temperature to obtain the ceramide composition with the skin repairing and thickening barrier.

Example 2

(1) Mixing 100g of glycerol, 50g of hydrogenated lecithin and 750g of purified water, stirring and heating to 65 ℃, keeping for 1h until the mixture is completely dissolved, and uniformly stirring to obtain a phase A;

(2) Mixing 100g of caprylic/capric triglyceride, 20g of octyl dodecanol, 20g of behenyl alcohol, 50g of ceramide NP, 20g of ceramide NS/ceramide NG and 20g of ceramide AP, heating to 85 ℃, keeping for 1h until the mixture is completely dissolved, and uniformly stirring to obtain a phase B;

(3) Mixing the phase A and the phase B again to obtain a mixed phase;

(4) Under the stirring condition, controlling the temperature of a system to be 45 ℃, adding 50g of squalane, 50g of shea butter, 50g of polyglycerol-3 methyl glucose distearate, 30g of bisabolol, 30g of purslane extract, 1g of sodium hyaluronate, 50g of beta-glucan and 100g of butanediol into the mixed phase, stirring for 1h, and then aging for 20h at room temperature to obtain the ceramide composition with the skin repairing and thickening barrier.

Example 3

(1) 55g of glycerol, 25g of hydrogenated lecithin and 600g of purified water are mixed, stirred and heated to 62 ℃, kept for 0.7h until the mixture is completely dissolved, and then stirred uniformly to obtain a phase A;

(2) Mixing 50g of caprylic/capric triglyceride, 10g of octyl dodecanol, 10g of behenyl alcohol, 25g of ceramide NP, 10g of ceramide NS/ceramide NG and 10g of ceramide AP, heating to 80 ℃, keeping for 0.7h until the mixture is completely dissolved, and uniformly stirring to obtain a phase B;

(3) Mixing the phase A and the phase B again to obtain a mixed phase;

(4) Under the stirring condition, controlling the temperature of a system to be 42 ℃, adding 30g of squalane, 30g of shea butter, 30g of polyglycerol-3 methyl glucose distearate, 15g of bisabolol, 15g of purslane extract, 0.5g of sodium hyaluronate, 25g of beta-glucan and 55g of butanediol into the mixed phase, stirring for 0.7h, and then aging for 17h at room temperature to obtain the ceramide composition with the skin repairing and thickening barrier.

Comparative verification example

First, simulate the biological transdermal test

The ceramide has the characteristics of high melting point, water insolubility, difficult emulsification and the like, and cannot be added in high concentration in the prior art, so that the transdermal effect of the traditional ceramide product is poor. The invention can obtain several combinations of hydrogenated lecithin, caprylic/capric triglyceride, octyl dodecanol, behenyl alcohol, ceramide NP, ceramide NS/ceramide NG and ceramide AP, can complete liposome and achieve transdermal effect, and has better transdermal effect compared with simple ceramide oil.

FIG. 1 is a graph showing a comparison of the biomimetic transdermal effects of simple ceramide oil and the composition (liposome) obtained in example 1 of the present invention. From the figure, it can be seen that the transdermal effect of the encapsulated liposome combination is significantly better than that of the uncoated ceramide.

The ceramide content in the skin is detected by an instrument, and the result is shown in figure 2, and the encapsulated ceramide liposome composition can be obviously remained in the stratum corneum, so that the repairing effect is achieved. Meanwhile, the skin layer cannot be reached through the stratum corneum, so that the safety is ensured.

(II) skin Barrier and sebum film thickening experiments

The present invention originally detects that the skin barrier (stratum corneum) to be repaired and broken needs different types of ceramide combinations, and the present invention carries out composition formulation by simulating the types and the content of natural ceramide in the skin. In addition, intercellular lipids of keratinocytes, besides ceramides, also fatty acids and cholesterol, are supplemented with closed "mortar" from natural sources, shea butter and squalane. According to the invention, through different repair experiments, the properties of the ceramide composition for repairing skin barriers and thickening sebum membranes are verified.

(1) Test sample

Test article: a packaged ceramide composition (example 1);

Control: blank control, front-to-back control;

The using method comprises the following steps: after the face is cleaned, a proper amount of the product is smeared on the face and the neck, and the product is tapped until the product is absorbed;

test part: forehead and cheek.

(2) Purpose of testing

At least 30 subjects meeting the standard are recruited, and the soothing, repairing and moisturizing effects of the products are judged by an instrument testing method by using the test products.

(3) The test method is based on

The method comprises the following steps: an instrumental analysis;

According to the following: cosmetic repair efficacy test method, cosmetic relief efficacy test method, cosmetic moisturizing efficacy test method.

(4) Test instrument

The facial Image analysis system VISIA-CR, image analysis software Image-Pro Plus7.0 IPP, transepidermal water loss test probe TEWAMETER TM Hex, melanin and heme detection probe Mexameter MX, stratum corneum moisture content test probe Corneometer CM825, and skin ultrasound tester UC22.

(5) Test index (5.1) efficacy tests are shown in table 1 (all test sites were faces).

TABLE 1

(6) Data statistics

The measured values of each test area are counted, including the number, the mean value, the standard deviation, the standard error, the minimum value, the median, the maximum value and the like. If the test data are normally distributed, adopting a pairing t-test method to carry out statistical analysis; if the test data are in non-normal distribution, adopting a rank sum test method to carry out statistical analysis.

The statistical methods all use a two-tailed test with a test level α=0.05.

Data statistics were analyzed statistically using SPSS25.0 software. When significant differences in results occur, p <0.05 and p <0.01 are noted, respectively. * p <0.05 represents a significant difference, p <0.01 represents a very significant difference. If the index values of the test areas have significant differences, the product has the effects of relieving, repairing and moisturizing.

(7) The test flow is shown in Table 2

TABLE 2

(8) Test results

(8.1) Analysis of the TEWL value for the transepidermal Water loss of skin

The larger the TEWL value measured by TEWAMETER TM Hex, the more transepidermal water loss per unit time, per unit cross-sectional area, and vice versa. The trend of reduced TEWL values thus represents a restoration process of transepidermal water loss from barrier damaged skin. By comparing the variation of the average value of the skin TEWL before and after use, the effect of the product to reduce skin moisture loss can be reflected.

I.e. the more the value of the TEWL mean at the different return visit time points drops compared to the pre-use baseline value (DO), the better the repair effect of the product is suggested.

TEWL value change rate = (post-product TEWL value used-pre-product TEWL value used)/pre-product TEWL value used x 100%.

The test results are shown in table 3, and the corresponding data graph is shown in fig. 3.

TABLE 3 analysis of the variance of the mean values of the skin TEWL (g/(h.m ²), x+ -s, n=32)

Group of	Mean ± standard deviation	P-value (compared with D0)
			D0	22.62±4.59	/
D14	19.56±2.77	＜0.01
			D28	16.94±3.01	＜0.01

Note that: n represents the number of people, and the same applies below.

As can be seen from table 3 and fig. 3: the skin TEWL mean showed a decreasing trend after product use compared to the pre-use baseline value (D0), 13.51% and 25.13% decrease after 14 and 28 days of use (D14 and D28), respectively, with a very significant difference (p < 0.01) compared to the baseline value, and the skin TEWL mean was below the baseline value after 14 and 28 days of product use.

(8.2) Analysis of skin heme (EI) values

The heme EI value characterizes the heme content of skin, and the lower the value measured by Mexameter MX 18, the lower the skin heme content at that location. The soothing effect of the product can be reflected by comparing the skin heme value changes before and after use.

That is, the more the value of heme value at different return visit time points is reduced compared with the baseline value (D0) before use, the better the improvement effect of skin redness is, and the better the redness relieving effect of the product is indicated.

Heme value change rate= (post-product heme value used-pre-product heme value used)/pre-product heme value used x 100%.

The test results are shown in table 4, and the corresponding data graph is shown in fig. 4.

Table 4 cutaneous heme differential analysis (x±s, n=32)

Group of	Mean ± standard deviation	P-value (compared with D0)
			D0	319.43±56.42	/
D14	280.35±48.46	＜0.01
			D28	240.98±43.28	＜0.01

As can be seen from table 4 and fig. 4: the skin heme EI values showed a decreasing trend after the product was used compared to the pre-use baseline value (D0), 12.23% and 24.56% decrease after 14 and 28 days (D14 and D28), respectively, with very significant differences (p < 0.01) compared to the baseline value, and skin heme EI values were below the baseline value after 14 and 28 days of the product was used.

(8.3) Analysis of skin red area

The red region Image shot by the VISIA-CR is subjected to color spot analysis in an Image Pro Plus software skin comprehensive analysis module, so that the change of the area ratio of the red region of the skin in AOI selected areas (radius is 3.5 cm) at different time points can be measured. The soothing effect of the product can be reflected by comparing the change of the area ratio of the red areas of the skin before and after use.

That is, the more the red area ratio decreases at different return visit time points compared with the baseline value (D0) before use, the smaller the skin redness area, which indicates that the better the redness relieving effect of the product is.

Rate of change in red area ratio = (red area ratio after use of product-red area ratio before use of product)/red area ratio before use of product x 100%.

The test results are shown in table 5, and the corresponding data graph is shown in fig. 5.

Table 5 analysis of the area-to-red area of skin differential (%), x±s, n=32

Group of	Mean ± standard deviation	P-value (compared with D0)
			D0	50.10±12.99	/
D14	35.19±12.82	＜0.01
			D28	35.91±10.60	＜0.01

As can be seen from table 5 and fig. 5: the red area ratio in the specific area of the face showed a decreasing trend after using the product compared with the baseline value before using (D0), 29.77% and 28.34% decrease after using the product for 14 days and 28 days (D14 and D28), respectively, and the red area ratio in the specific area of the face was lower than the baseline value after using the product for 14 days and 28 days with a very significant difference (p < 0.01) compared with the baseline value.

In order to more intuitively express the effect, the present invention selects a partial red region map of the VISIA of two subjects (subject 13 and subject 16), as shown in fig. 6 and 7, which also show: the area of the red area of the skin was below the baseline value at both 14 and 28 days of use.

(8.4) Analysis of moisture content of skin stratum corneum

The higher the moisture content value of the skin stratum corneum, as measured by Corneometer CM825, the higher the moisture content of the skin stratum corneum. By comparing the change in the moisture content of the stratum corneum in a specific area before and after use, the effect of the product on the moisture content of the stratum corneum can be reflected.

That is, the more the moisture content increases at different return visit time points compared with the baseline value (D0) before use, the better the improvement effect of the moisture content of the inner surface of the test area, which indicates that the better the effect of the product for increasing the moisture content of the skin cuticle.

Skin moisture content change rate= (moisture content after use of product-moisture content before use of product)/moisture content before use of product x 100%.

The test results are shown in table 6, and the corresponding data graph is shown in fig. 8.

Table 6 analysis of moisture content differences in skin stratum corneum (c.u., x±s, n=32)

Group of	Mean ± standard deviation	P-value (compared with D0)
			D0	40.90±7.14	/
D14	50.65±8.66	＜0.01
			D28	59.88±7.59	＜0.01

As can be seen from table 6 and fig. 8: the skin stratum corneum moisture content showed an upward trend after the use of the product compared to the baseline value before the use (D0), up by 23.83% and 46.39% after the use of the product for 14 days and 28 days (D14 and D28), respectively, and had a very significant difference (p < 0.01) compared to the baseline value, and the skin stratum corneum moisture content was higher than the baseline value after the use of the product for 14 days and 28 days.

(8.5) Analysis of epidermal layer thickness

The cheek skin ultrasonic image scanned by the UC22 skin ultrasonic tester is analyzed by software, and the thickness change of the skin epidermis layer at different time points can be measured. By comparing the thickness change of the epidermis before and after use, the effect of the product on the epidermis of the skin can be reflected.

That is, the more the value of the skin layer thickness at different return visit time points is increased compared with the baseline value (D0) before use, the larger the skin layer thickness is, which suggests that the better the effect of the product on enhancing the skin layer thickness and improving the skin barrier is.

Skin thickness change rate = (skin thickness after product use-skin thickness before product use)/skin thickness before product use x 100%.

The test results are shown in table 7, and the corresponding data graph is shown in fig. 9.

Table 7 analysis of the thickness differences of the epidermis layers (μm., x±s, n=32)

As can be seen from table 7 and fig. 9: the facial skin layer thickness showed an upward trend after 28 days of use of the product compared to the pre-use baseline value (D0), 13.84% after 14 days of use (D14), with no significant difference (p > 0.05) compared to the baseline value; 51.99% rise after 28 days of use (D28), with a very significant difference (p < 0.01) from the baseline value, and skin layer thickness above the baseline value after 28 days of use of the product.

In addition, in order to more intuitively express the effect, the invention selects an ultrasonic scan of a subject (code number is subject 18), as shown in fig. 10, which shows: the thickness of the epidermis layer after 28 days of use of the product is significantly higher than the baseline value.

The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A ceramide composition having a repaired, thickened skin barrier, comprising a phase a, a phase B, and a phase C; wherein:

2. The ceramide composition with repair and thickening of skin barrier according to claim 1, wherein the a phase comprises the following raw materials in parts by weight: 5.5 parts of glycerol, 2.5 parts of hydrogenated lecithin and 60 parts of purified water.

3. The ceramide composition with repair and thickening of skin barrier according to claim 1, wherein the B phase comprises the following raw materials in parts by weight: 5 parts of caprylic/capric triglyceride, 1 part of octyl dodecanol, 1 part of behenyl alcohol, 2.5 parts of ceramide NP, 1 part of ceramide NS/ceramide NG and 1 part of ceramide AP.

4. The ceramide composition with repair and thickening of skin barrier of claim 1, wherein the C phase comprises the following raw materials in parts by weight: 3 parts of squalane, 3 parts of butter fruit tree fruit fat, 3 parts of polyglycerol-3-methyl glucose distearate, 1.5 parts of bisabolol, 1.5 parts of purslane extract, 0.05 part of sodium hyaluronate, 2.5 parts of beta-glucan and 5.5 parts of butanediol.

5. A method for preparing a ceramide composition having a repair and thickening skin barrier according to any one of claims 1 to 4, characterized in that the preparation method comprises the steps of:

(3) Mixing the phase A and the phase B again to obtain a mixed phase;

6. The preparation method according to claim 5, wherein in the step (1), glycerin, hydrogenated lecithin and purified water are mixed, heated to 60 to 65 ℃ and kept for 0.5 to 1 hour until completely dissolved, and then stirred uniformly to obtain phase A.

7. The preparation method according to claim 5, wherein in the step (2), caprylic/capric triglyceride, octyldodecanol, behenyl alcohol, ceramide NP, ceramide NS/ceramide NG and ceramide AP are mixed, heated to 75-85 ℃, kept for 0.5-1 h until being completely dissolved, and then stirred uniformly to obtain phase B.

8. The preparation method according to claim 5, wherein in the step (4), squalane, shea butter, polyglycerol-3 methyl glucose distearate, bisabolol, purslane extract, sodium hyaluronate, beta-glucan and butanediol are added into the mixed phase under the condition of stirring, the temperature of the system is controlled to be 40-45 ℃, the mixture is stirred for 0.5-1 h, and then the mixture is aged for 15-20 h at room temperature, so that the ceramide composition with the skin barrier repairing and thickening functions is obtained.