CN116407476A - Composition for removing dandruff, relieving itching and soothing, and preparation method and application thereof - Google Patents

Abstract

The invention provides a composition for removing dandruff, relieving itching and soothing, a preparation method and application thereof. The composition for removing dandruff, relieving itching and relieving can be added into any shampoo and skin care product for use, and the extract provided by the invention is matched with reasonable proportion, and has high content of active ingredients and excellent effects of removing dandruff, relieving itching and relieving. The three plants are extracted and fermented, so that the antibacterial and anti-inflammatory effects of the three components are exerted to the greatest extent, the obtained composition has remarkable antibacterial effect, is convenient to popularize and apply to shampoos in a large range, has a very good anti-dandruff and antipruritic effect, is warm and comfortable in action, and has an excellent soothing effect on skin.

Description

Antidandruff, antipruritic, soothing composition and its preparation method and application

technical field

The invention belongs to the technical field of A61K, and in particular relates to a soothing composition; an anti-dandruff, antipruritic, soothing composition and application thereof.

Background technique

According to the incomplete statistics of the World Health Organization, the incidence rate of dandruff is above 65%. According to the relevant data survey in my country, the incidence rate of teenagers is above 70%. The normal work and life were seriously affected by the illness. The mainstream of existing hair shampoos is chemical products, and chemical raw materials produce a certain stimulating effect on human head skin, which aggravates the occurrence of scalp pruritus and dandruff on the other hand. External medicine for dandruff is a kind of medicine that has developed rapidly in the market in recent years. According to statistics, the total sales of external medicine for dandruff in 2010 was about 18.5 billion. There is an urgent market demand for dandruff removal products.

At present, the anti-dandruff and anti-pruritic agents used in anti-dandruff washing and care products in the domestic market are basically synthetic chemical substances such as ZPT (Zinc Omatin), Campanolin and the like. Although these substances have certain anti-dandruff and anti-itch effects, the anti-dandruff effect is not ideal, and there is a certain irritation. Long-term use may affect the scalp and cause other hair problems. Therefore, there is a need for a shampoo composition that has anti-dandruff and antipruritic effects and is mild to the scalp and hair.

Contents of the invention

The purpose of the present invention is to provide a composition with plant extract as the core anti-dandruff component, which has the effects of anti-dandruff, antipruritic and soothing.

In order to achieve the above object, the present invention provides the following technical solutions:

The first aspect of the present invention provides an anti-dandruff, antipruritic and soothing composition, the composition includes Leaf Extract of Phyllostachys miltiorrhiza, Extract of Andrographis paniculata Leaf and Bark Bark Extract of Phellodendron The mass ratio of the leaf extract and the bark extract of Phellodendron bark is 1:(1-6):(1-20).

Preferably, the composition includes Leaf Extract of Phyllostachys miltiorrhiza, Leaf Extract of Andrographis paniculata and Bark Bark Extract of Phellodendron bark, and the mass ratio of the leaf extract of Phyllostachys miltiorrhiza, extract of leaves of Andrographis paniculata and bark of Phellodendron bark is 1:( 1～3): (2～5).

Preferably, the mass ratio of the leaf extract of Phyllostachys miltiorrhiza, the extract of leaves of Andrographis paniculata and the bark of Phellodendron bark is 1:1.5:3.6.

According to the present invention, by compounding the three substances of the leaf extract of the phlox, the leaf extract of the Andrographis paniculata and the bark extract of the bark bark, the composition obtained has the effect of anti-dandruff and antipruritic and is mild to the scalp and hair, and is suitable for adding to hair shampoo In water, the anti-dandruff and anti-itch effect is obvious.

The third aspect of the present invention provides the preparation method of the anti-dandruff, antipruritic and soothing composition, which is characterized in that it comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grind the dried leaves of Pachyphyllum edulis, heat and extract with a mixed alcohol composed of methanol and ethanol, the volume ratio of methanol and ethanol is 2-5:1, to obtain the extract, and concentrate at normal pressure , drying to obtain the leaf extract of Bamboo basilica;

Preparation of Andrographis paniculata leaf extract: Grind andrographis paniculata leaves, soak in distilled water for 30-60 minutes, preheat at 40-50°C for 1-2 hours, then heat to boil and keep for 2-3 hours Afterwards, the extract was obtained, concentrated and dried to obtain the Andrographis paniculata leaf extract;

Preparation of Phellodendron bark extract: Grind the bark of Phellodendron bark, add distilled water A and stir evenly, soak for 1-2 hours, then heat to boiling, keep for 60-90 minutes, filter to obtain medicinal residues and initial extract; add medicinal residues to Distilled water B, heated to boiling, kept for 20-30 minutes, filtered to obtain the re-extracted liquid; combined the initial extracted liquid and the re-extracted liquid, concentrated and dried to obtain the bark extract of Phellodendron bark;

(2) fermentation

Mix the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark extract of Phellodendron bark to obtain a mixture, add the carbon source and the mixture to sterile water, use PBS buffer solution to adjust the pH to 6.9-7.1, and sterilize with ultraviolet light to obtain a fermentation medium ; Inoculate Bacillus subtilis and Acetobacter pasteurianus into Gaulian culture medium for 12-18 hours respectively, and culture them into strain seed liquid respectively; Ferment and cultivate for 35-45 hours, filter, and freeze-dry the fermented liquid to obtain an antidandruff, antipruritic and soothing composition.

Preferably, the weight ratio of the light bamboo leaf powder to the mixed alcohol is 1:8-15, and the extraction time is 30-60 minutes.

Preferably, the leaves of the bamboo leaves are ground and passed through a 300-400 mesh sieve.

Preferably, the leaf extract of Phyllostachys edulis is extracted with a Soxhlet extractor.

Preferably, the volume ratio of the methanol to ethanol is 3:1, the weight ratio of the light bamboo leaf powder to the mixed alcohol is 1:10, and the extraction time is 40 minutes.

In the present invention, the extract obtained by using complex alcohols (methanol and ethanol) to extract the leaf extract of Pachyphyllum edulis has excellent effects, and when the volume ratio of methanol and ethanol is 3:1, the most active ingredients for anti-dandruff and anti-itching can be obtained, and the effect is the best. The flavonoids in the leaves of the light bamboo leaves have an antibacterial effect, and the extraction method of the present invention can obtain the most effective antibacterial components. The inventors found that the amount of alcohol added also affects the extraction effect.

Andrographis paniculata has the effects of clearing away heat and detoxification, reducing swelling and relieving pain. In recent years, Andrographis paniculata has received more and more applications and attention due to its extensive resources and definite curative effect. However, due to its unstable internal quality and insufficient extraction process, it affects the development and application of its products to a certain extent. The present invention uses the leaves of Andrographis paniculata for extraction. The main components in Andrographis paniculata are diterpene lactones, and the secondary components are flavonoids, which have antibacterial effects. At the same time, the extraction method of the present invention has many active ingredients. The antibacterial effect of the ester compound is related to the position of the double bond of the andrographolide compound, and the extract of the present invention contains the andrographolide compound with a double bond in the ring, which has a strong antibacterial effect, and it can be combined with other extracts. The antibacterial ingredients work together to play an antibacterial effect, greatly improving the antibacterial effect.

Patent CN 115089516 A discloses a compound plant extract with anti-dandruff and antipruritic effects and its preparation method, and discloses the preparation method of Phellodendron bark extract, wherein the flavonoids and alkaloids have antibacterial and anti-inflammatory effects, The inventors found that the effect of this method in the composition of the present invention is not ideal, and the patent patent CN 115089516A re-extracts the initial extraction liquid may lose part of the effective antibacterial components. The present invention obtains the More active ingredients. In addition, the extraction conditions of the present invention also affect the extraction effect.

Preferably, the volume ratio of the leaves of Andrographis paniculata and distilled water is 1:10-12; the volume ratio of the bark of Phellodendron amurense and distilled water A is 1:15-20; the volume ratio of the medicinal residues and distilled water B is 1: 10-12.

In order to improve the effect of extract fermentation, the carbon source is selected from one or more of glucose, fructose, mannose, galactose; the mass ratio of the carbon source, mixture and sterile water is (10-20) : (10-15): 75; the aerobic culture of the Acetobacter pasteurianus, the temperature is 35～38 ℃, the oxygen content is 18～22%, and the humidity is 70～75%; the anaerobic culture of the Bacillus subtilis, The temperature is 35-38°C, and the humidity is 70-75%. The inoculation amount of Acetobacter pasteurii is 2-5%. The inoculum amount of Bacillus subtilis is 2-5%.

Preferably, the leaves of Andrographis paniculata and bark of Phellodendron bark are ground and passed through a 300-400 mesh sieve.

The present invention ferments the leaf extracts of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark of Phellodendron bark, and the extract of leaves of Phyllostachys miltiorrhiza, the extract of leaves of Andrographis paniculata and the bark of Phellodendron amurense. Precipitation occurs, which affects the appearance and use effect of the product. The polysaccharides contained are easy to combine with flavonoids and exist in the form of glycosides, which will reduce the antibacterial effect of flavonoids. Therefore, the inventors carried out fermentation treatment to improve the antibacterial properties of the components. sex.

The fourth aspect of the present invention provides the application of the anti-dandruff, anti-itch and soothing composition in the preparation of shampoo.

Preferably, the added amount of the anti-dandruff, anti-itch and soothing composition is 0.1wt%-10wt%.

Compared with prior art, advantage and beneficial effect of the present invention are:

The invention provides a soothing composition, an anti-dandruff, antipruritic, soothing composition and application thereof. The composition includes extracts of bamboo leaves, extracts of andrographis paniculata leaves and bark extracts of Phellodendron bark. The anti-dandruff, antipruritic and soothing composition provided by the present invention can be added to any basic shampoo or skin care product for use, and the extract of the present invention is used in a reasonable proportion and obtained through a special preparation method The content of active ingredients is high, and it has excellent anti-dandruff, anti-itching and soothing effects. The present invention maximizes the antibacterial and anti-inflammatory effects of the three ingredients by extracting and fermenting the three kinds of plants, and the obtained composition has remarkable antibacterial effect, which is convenient for wide-scale popularization and application in shampoo, and has very good The anti-dandruff and anti-itch effect is mild and comfortable, and has an excellent soothing effect on the skin. The anti-dandruff, anti-itch and soothing composition of the present invention can realize anti-dandruff from the source without recurrence, cleans the scalp, and maintains the micro-ecological balance of the scalp. The minimalist formula can be applied to sensitive scalps, keeping users away from scalp itching, keeping the scalp clean, gentle and soothing, and keeping the hair soft and shiny, which is of great significance to the development of anti-dandruff shampoos.

Description of drawings

Fig. 1 is a diagram of the effect of adding the composition of Example 1 to shampoo, wherein the front diagram is the effect before use, and the rear diagram is the effect after use.

Fig. 2 is a diagram of the effect of adding the composition of Example 1 to the face cream, wherein the front diagram is the effect before use, and the rear diagram is the effect after use.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

Example 1

This example provides an anti-dandruff, antipruritic and soothing composition, which includes extracts of Bambusa edulis leaves, extracts of Andrographis paniculata and bark of Phellodendron bark, in a mass ratio of 1:1.5:3.6.

The preparation method of described a kind of anti-dandruff, antipruritic, soothing composition, comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grind the dried leaves of Pachyphyllum edulis, heat and extract with a mixed alcohol composed of methanol and ethanol, the volume ratio of methanol and ethanol is 3:1, obtain the extract, concentrate at normal pressure, and dry dry to obtain the leaf extract of Pachyphyllum edulis; the weight ratio of the leaf powder of Pachyphyllum edulis and mixed alcohol is 1:10, and the extraction time is 40 minutes.

Preparation of Andrographis paniculata leaf extract: Grind Andrographis paniculata leaves, soak in distilled water for 40 minutes, preheat for 1.5 hours at 45°C, then heat to boiling and keep for 2.5 hours to obtain the extract, concentrate, drying to obtain Andrographis paniculata leaf extract; the volume ratio of the Andrographis paniculata leaves and distilled water is 1:11;

Preparation of Phellodendron bark extract: Grind the bark of Phellodendron bark, add distilled water and stir evenly, soak for 1.5h, then heat to boiling, keep for 80min, filter to obtain medicinal residue and initial extract; add medicinal residue to distilled water, heat to Boiling, kept for 25min, filtered to obtain the re-extracted liquid; the initial extracted liquid and the re-extracted liquid were combined, concentrated and dried to obtain the bark extract of Phellodendron bark; the volume ratio of bark of Phellodendron bark and distilled water: 1:17; The volume ratio of medicinal residues and distilled water: 1:11.

(2) fermentation

Mixing the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark of Phellodendron bark extract to obtain a mixture, the carbon source and the mixture are added to sterile water, the carbon source is glucose and fructose with a weight ratio of 8:2, and the carbon source The mass ratio of the source, the mixture and sterile water is 15:13:75, the pH is adjusted to 6.9 with PBS buffer solution, and ultraviolet sterilized to obtain the fermentation medium. Inoculate Bacillus subtilis and Acetobacter pasteurianus respectively into the Goosei medium, cultivate Acetobacter pasteurii aerobically at 37°C, oxygen content 20%, humidity 75%, Bacillus subtilis anaerobic culture, temperature At 37°C, in an anaerobic environment, at a humidity of 75%, and for 14 hours, the cultures were respectively cultured into bacterial seed liquids with a bacterial content of 120cfu/mL. Inoculate the strain seed liquid into the fermentation medium, the inoculum amount of Acetobacter pasteurianus is 3%; Dandruff, antipruritic, soothing composition.

Example 2

This example provides an anti-dandruff, antipruritic and soothing composition, which includes extracts of Bambusa edulis leaves, extracts of Andrographis paniculata and bark of Phellodendron bark, in a mass ratio of 1:1.5:3.6.

The preparation method of described a kind of anti-dandruff, antipruritic, soothing composition, comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grinding the leaves of Pachyphyllum edulis, soaking in distilled water for 40 minutes, preheating at 45°C for 1.5 hours, then heating to boiling and maintaining for 2.5 hours to obtain the extract, Concentrating and drying to obtain the leaf extract of Pachyphyllum edulis; the volume ratio of the leaves and distilled water is 1:11;

Preparation of Andrographis paniculata leaf extract: Grind Andrographis paniculata leaves, soak in distilled water for 40 minutes, preheat for 1.5 hours at 45°C, then heat to boiling and keep for 2.5 hours to obtain the extract, concentrate, drying to obtain Andrographis paniculata leaf extract; the volume ratio of the Andrographis paniculata leaves and distilled water is 1:11;

Preparation of Phellodendron bark extract: Grind the bark of Phellodendron bark, add distilled water and stir evenly, soak for 1.5h, then heat to boiling, keep for 80min, filter to obtain medicinal residue and initial extract; add medicinal residue to distilled water, heat to Boiling, kept for 25min, filtered to obtain the re-extracted liquid; the initial extracted liquid and the re-extracted liquid were combined, concentrated and dried to obtain the bark extract of Phellodendron bark; the volume ratio of bark of Phellodendron bark and distilled water: 1:17; The volume ratio of medicinal residues and distilled water: 1:11.

(2) fermentation

Mixing the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark of Phellodendron bark extract to obtain a mixture, the carbon source and the mixture are added to sterile water, the carbon source is glucose and fructose with a weight ratio of 8:2, and the carbon source The mass ratio of the source, the mixture and sterile water is 15:13:75, the pH is adjusted to 6.9 with PBS buffer solution, and ultraviolet sterilized to obtain the fermentation medium. Inoculate Bacillus subtilis and Acetobacter pasteurianus respectively into the Goosei medium, cultivate Acetobacter pasteurii aerobically at 37°C, oxygen content 20%, humidity 75%, Bacillus subtilis anaerobic culture, temperature At 37°C, in an anaerobic environment, at a humidity of 75%, and for 14 hours, the cultures were respectively cultured into bacterial seed liquids with a bacterial content of 120cfu/mL. Inoculate the strain seed liquid into the fermentation medium, the inoculum amount of Acetobacter pasteurianus is 3%; Dandruff, antipruritic, soothing composition.

Example 3

This example provides an anti-dandruff, antipruritic and soothing composition, which includes extracts of Bambusa edulis leaves, extracts of Andrographis paniculata and bark of Phellodendron bark, in a mass ratio of 1:1.5:3.6. .

The preparation method of described a kind of anti-dandruff, antipruritic, soothing composition, comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grinding the dried leaves of Pachyphyllum edulis, heating and extracting with ethanol to obtain the extract, concentrating and drying under normal pressure to obtain the leaf extract of Pachyphyllum edulis; The weight ratio is 1:10, and the extraction time is 40 minutes.

Preparation of Andrographis paniculata leaf extract: Grind Andrographis paniculata leaves, soak in distilled water for 40 minutes, preheat for 1.5 hours at 45°C, then heat to boiling and keep for 2.5 hours to obtain the extract, concentrate, drying to obtain Andrographis paniculata leaf extract; the volume ratio of the Andrographis paniculata leaves and distilled water is 1:11;

Preparation of Phellodendron bark extract: Grind the bark of Phellodendron bark, add distilled water and stir evenly, soak for 1.5h, then heat to boiling, keep for 80min, filter to obtain medicinal residue and initial extract; add medicinal residue to distilled water, heat to Boiling, kept for 25min, filtered to obtain the re-extracted liquid; the initial extracted liquid and the re-extracted liquid were combined, concentrated and dried to obtain the bark extract of Phellodendron bark; the volume ratio of bark of Phellodendron bark and distilled water: 1:17; The volume ratio of medicinal residues and distilled water: 1:11.

(2) fermentation

Mixing the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark of Phellodendron bark extract to obtain a mixture, the carbon source and the mixture are added to sterile water, the carbon source is glucose and fructose with a weight ratio of 8:2, and the carbon source The mass ratio of the source, the mixture and sterile water is 15:13:75, the pH is adjusted to 6.9 with PBS buffer solution, and ultraviolet sterilized to obtain the fermentation medium. Inoculate Bacillus subtilis and Acetobacter pasteurianus respectively into the Goosei medium, cultivate Acetobacter pasteurii aerobically at 37°C, oxygen content 20%, humidity 75%, Bacillus subtilis anaerobic culture, temperature At 37°C, in an anaerobic environment, at a humidity of 75%, and for 14 hours, the cultures were respectively cultured into bacterial seed liquids with a bacterial content of 120cfu/mL. Inoculate the strain seed liquid into the fermentation medium, the inoculum amount of Acetobacter pasteurianus is 3%; Dandruff, antipruritic, soothing composition.

Example 4

This example provides an anti-dandruff, antipruritic and soothing composition, which includes the leaf extract of Phyllostachys edulis, the extract of Andrographis paniculata and the bark extract of Phellodendron bark, with a mass ratio of 3:10:1.

The preparation method of described a kind of anti-dandruff, antipruritic, soothing composition, comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grind the dried leaves of Pachyphyllum edulis, heat and extract with a mixed alcohol composed of methanol and ethanol, the volume ratio of methanol and ethanol is 3:1, obtain the extract, concentrate at normal pressure, and dry dry to obtain the leaf extract of Pachyphyllum edulis; the weight ratio of the leaf powder of Pachyphyllum edulis and mixed alcohol is 1:10, and the extraction time is 40 minutes.

Preparation of Andrographis paniculata leaf extract: Grind Andrographis paniculata leaves, soak in distilled water for 40 minutes, preheat for 1.5 hours at 45°C, then heat to boiling and keep for 2.5 hours to obtain the extract, concentrate, drying to obtain Andrographis paniculata leaf extract; the volume ratio of the Andrographis paniculata leaves and distilled water is 1:11;

Preparation of Phellodendron bark extract: Grind the bark of Phellodendron bark, add distilled water and stir evenly, soak for 1.5h, then heat to boiling, keep for 80min, filter to obtain medicinal residue and initial extract; add medicinal residue to distilled water, heat to Boiling, kept for 25min, filtered to obtain the re-extracted liquid; the initial extracted liquid and the re-extracted liquid were combined, concentrated and dried to obtain the bark extract of Phellodendron bark; the volume ratio of bark of Phellodendron bark and distilled water: 1:17; The volume ratio of medicinal residues and distilled water: 1:11.

(2) fermentation

Mixing the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark of Phellodendron bark extract to obtain a mixture, the carbon source and the mixture are added to sterile water, the carbon source is glucose and fructose with a weight ratio of 8:2, and the carbon source The mass ratio of the source, the mixture and sterile water is 15:13:75, the pH is adjusted to 6.9 with PBS buffer solution, and ultraviolet sterilized to obtain the fermentation medium. Inoculate Bacillus subtilis and Acetobacter pasteurianus respectively into the Goosei medium, cultivate Acetobacter pasteurii aerobically at 37°C, oxygen content 20%, humidity 75%, Bacillus subtilis anaerobic culture, temperature At 37°C, in an anaerobic environment, at a humidity of 75%, and for 14 hours, the cultures were respectively cultured into bacterial seed liquids with a bacterial content of 120cfu/mL. Inoculate the strain seed liquid into the fermentation medium, the inoculum amount of Acetobacter pasteurianus is 3%; Dandruff, antipruritic, soothing composition.

Example 5

This example provides an anti-dandruff, antipruritic and soothing composition, which includes extracts of Bambusa edulis leaves, extracts of Andrographis paniculata and bark of Phellodendron bark, in a mass ratio of 1:1.5:3.6. .

The preparation method of described a kind of anti-dandruff, antipruritic, soothing composition, comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grind the dried leaves of Pachyphyllum edulis, heat and extract with a mixed alcohol composed of methanol and ethanol, the volume ratio of methanol and ethanol is 3:1, obtain the extract, concentrate at normal pressure, and dry dry to obtain the leaf extract of Pachyphyllum edulis; the weight ratio of the leaf powder of Pachyphyllum edulis and mixed alcohol is 1:10, and the extraction time is 40 minutes.

Preparation of Andrographis paniculata leaf extract: Grind Andrographis paniculata leaves, soak in distilled water for 40 minutes, preheat for 1.5 hours at 45°C, then heat to boiling and keep for 2.5 hours to obtain the extract, concentrate, drying to obtain Andrographis paniculata leaf extract; the volume ratio of the Andrographis paniculata leaves and distilled water is 1:11;

Preparation of Phellodendron bark extract: Grind the bark of Phellodendron bark, add distilled water and stir evenly, soak for 1.5h, then heat to boiling, keep for 80min, filter to obtain medicinal residue and initial extract; add medicinal residue to distilled water, heat to Boiling, kept for 25min, filtered to obtain the re-extracted liquid; the initial extracted liquid and the re-extracted liquid were combined, concentrated and dried to obtain the bark extract of Phellodendron bark; the volume ratio of bark of Phellodendron bark and distilled water: 1:17; The volume ratio of medicinal residues and distilled water: 1:11.

(2) Mixing the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark extract of Phellodendron bark to obtain an anti-dandruff, antipruritic and soothing composition.

Example 6

This example provides an anti-dandruff, antipruritic and soothing composition, which includes extracts of Bambusa edulis leaves, extracts of Andrographis paniculata and bark of Phellodendron bark, in a mass ratio of 1:1.5:3.6. .

The preparation method of described a kind of anti-dandruff, antipruritic, soothing composition, comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grind the dried leaves of Pachyphyllum edulis, heat and extract with a mixed alcohol composed of methanol and ethanol, the volume ratio of methanol and ethanol is 3:1, obtain the extract, concentrate at normal pressure, and dry dry to obtain the leaf extract of Pachyphyllum edulis; the weight ratio of the leaf powder of Pachyphyllum edulis and mixed alcohol is 1:10, and the extraction time is 40 minutes.

Preparation of Andrographis paniculata leaf extract: Grind Andrographis paniculata leaves, soak in distilled water for 40 minutes, preheat for 1.5 hours at 45°C, then heat to boiling and keep for 2.5 hours to obtain the extract, concentrate, drying to obtain Andrographis paniculata leaf extract; the volume ratio of the Andrographis paniculata leaves and distilled water is 1:11;

Preparation of Phellodendron bark extract: After cutting and washing the bark of Phellodendron bark, add water for the first decoction (the specific operation is: add 10 times the amount of water of raw materials, soak for 1 hour, heat to boiling and decoct for 3 hours, use 100 mesh Filter out the initial extract of Phellodendron bark (2) and then extract: add water to the initial extract and decoct for the second time (the specific operation is: add 8 times the water of the initial extract, heat to boiling and decoct for 2 hours, respectively A 100-mesh sieve was used to filter out the re-extracted bark of Phellodendron bark; the re-extracted liquid was left standing for 2 hours, concentrated and dried to obtain the bark of Phellodendron bark extract.

(2) fermentation

Mixing the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark of Phellodendron bark extract to obtain a mixture, the carbon source and the mixture are added to sterile water, the carbon source is glucose and fructose with a weight ratio of 8:2, and the carbon source The mass ratio of the source, the mixture and sterile water is 15:13:75, the pH is adjusted to 6.9 with PBS buffer solution, and ultraviolet sterilized to obtain the fermentation medium. Inoculate Bacillus subtilis and Acetobacter pasteurianus respectively into the Goosei medium, cultivate Acetobacter pasteurii aerobically at 37°C, oxygen content 20%, humidity 75%, Bacillus subtilis anaerobic culture, temperature At 37°C, in an anaerobic environment, at a humidity of 75%, and for 14 hours, the cultures were respectively cultured into bacterial seed liquids with a bacterial content of 120cfu/mL. Inoculate the strain seed liquid into the fermentation medium, the inoculum amount of Acetobacter pasteurianus is 3%; Dandruff, antipruritic, soothing composition.

Example 7

This embodiment provides an anti-dandruff, antipruritic and soothing composition, the composition includes the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark extract of Phellodendron bark, and the mass ratio is 1:1.5:3.6 .

The preparation method of described a kind of anti-dandruff, antipruritic, soothing composition, comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grind the dried leaves of Pachyphyllum edulis, heat and extract with a mixed alcohol composed of methanol and ethanol, the volume ratio of methanol and ethanol is 3:1, obtain the extract, concentrate at normal pressure, and dry dry to obtain the leaf extract of Pachyphyllum edulis; the weight ratio of the leaf powder of Pachyphyllum edulis and mixed alcohol is 1:10, and the extraction time is 40 minutes.

Preparation of Andrographis paniculata root extract: Grind andrographis paniculata root, soak in distilled water for 40 minutes, preheat for 1.5 hours at 45°C, then heat to boiling and keep for 2.5 hours to obtain the extract, concentrate, Drying to obtain Andrographis paniculata root extract; the volume ratio of the Andrographis paniculata leaves and distilled water is 1:11;

Preparation of Phellodendron bark extract: Grind the bark of Phellodendron bark, add distilled water and stir evenly, soak for 1.5h, then heat to boiling, keep for 80min, filter to obtain medicinal residue and initial extract; add medicinal residue to distilled water, heat to Boiling, kept for 25min, filtered to obtain the re-extracted liquid; the initial extracted liquid and the re-extracted liquid were combined, concentrated and dried to obtain the bark extract of Phellodendron bark; the volume ratio of bark of Phellodendron bark and distilled water: 1:17; The volume ratio of medicinal residues and distilled water: 1:11.

(2) fermentation

Mixing the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark of Phellodendron bark extract to obtain a mixture, the carbon source and the mixture are added to sterile water, the carbon source is glucose and fructose with a weight ratio of 8:2, and the carbon source The mass ratio of the source, the mixture and sterile water is 15:13:75, the pH is adjusted to 6.9 with PBS buffer solution, and ultraviolet sterilized to obtain the fermentation medium. Inoculate Bacillus subtilis and Acetobacter pasteurianus respectively into the Goosei medium, cultivate Acetobacter pasteurii aerobically at 37°C, oxygen content 20%, humidity 75%, Bacillus subtilis anaerobic culture, temperature At 37°C, in an anaerobic environment, at a humidity of 75%, and for 14 hours, the cultures were respectively cultured into bacterial seed liquids with a bacterial content of 120cfu/mL. Inoculate the strain seed liquid into the fermentation medium, the inoculum amount of Acetobacter pasteurianus is 3%; Dandruff, antipruritic, soothing composition.

Example 8

This example provides an anti-dandruff, antipruritic and soothing composition, which includes extracts of Bambusa edulis leaves, extracts of Andrographis paniculata and bark of Phellodendron bark, in a mass ratio of 1:1.5:3.6.

The preparation method of described a kind of anti-dandruff, antipruritic, soothing composition, comprises the following steps:

(1) extraction

Preparation of the leaf extract of Pachyphyllum edulis: Grind the dried leaves of Pachyphyllum edulis, heat and extract with a mixed alcohol composed of methanol and ethanol, the volume ratio of methanol and ethanol is 3:1, obtain the extract, concentrate at normal pressure, and dry dry to obtain the leaf extract of Pachyphyllum edulis; the weight ratio of the leaf powder of Pachyphyllum edulis and mixed alcohol is 1:10, and the extraction time is 40 minutes.

Preparation of Andrographis paniculata leaf extract: Grind Andrographis paniculata leaves, soak in distilled water for 40 minutes, preheat for 1.5 hours at 45°C, then heat to boiling and keep for 2.5 hours to obtain the extract, concentrate, drying to obtain Andrographis paniculata leaf extract; the volume ratio of said Andrographis paniculata leaves and distilled water is 1:15;

Preparation of Phellodendron bark extract: Grind the bark of Phellodendron bark, add distilled water and stir evenly, soak for 1.5h, then heat to boiling, keep for 80min, filter to obtain medicinal residue and initial extract; add medicinal residue to distilled water, heat to Boiling, kept for 25min, filtered to obtain the re-extracted solution; the initial extracted solution and the re-extracted solution were combined, concentrated and dried to obtain the bark extract of Phellodendron bark; the volume ratio of bark of Phellodendron amurense and distilled water: 1:11; The volume ratio of dregs and distilled water: 1:15.

(2) fermentation

Mixing the leaf extract of Phyllostachys miltiorrhiza, the leaf extract of Andrographis paniculata and the bark of Phellodendron bark extract to obtain a mixture, the carbon source and the mixture are added to sterile water, the carbon source is glucose and fructose with a weight ratio of 8:2, and the carbon source The mass ratio of the source, the mixture and sterile water is 15:13:75, the pH is adjusted to 6.9 with PBS buffer solution, and ultraviolet sterilized to obtain the fermentation medium. Inoculate Bacillus subtilis and Acetobacter pasteurianus respectively into the Goosei medium, cultivate Acetobacter pasteurii aerobically at 37°C, oxygen content 20%, humidity 75%, Bacillus subtilis anaerobic culture, temperature At 37°C, in an anaerobic environment, at a humidity of 75%, and for 14 hours, the cultures were respectively cultured into bacterial seed liquids with a bacterial content of 120cfu/mL. Inoculate the strain seed liquid into the fermentation medium, the inoculum amount of Acetobacter pasteurianus is 3%; Dandruff, antipruritic, soothing composition.

Performance Testing:

1. Antibacterial rate

Most studies have shown that microbiological factors and local immune responses play a major role in the pathogenesis of dandruff. It is generally believed that the overgrowth of microorganisms mainly Malassezia furfur is one of the important factors.

The antibacterial test was carried out with reference to the light industry standard of the People's Republic of China QB/T 2738-2012 "Evaluation method for antibacterial and antibacterial effects of daily chemical products" in 7.3 Antibacterial effect test method of antibacterial daily chemical products (suspension quantitative method) test. Through the antibacterial test, it was judged that the tested sample was against Malassezia furfur. A bacteriostatic rate greater than 50% means that the product has antibacterial effect, that is, anti-dandruff effect, and the higher the bacteriostatic rate, the better the effect.

(1), materials and methods

equipment

Autoclave: Shanghai Lichen Bangxi Instrument Technology Co., Ltd., DGL-100B

Biological safety cabinet: Zhejiang Fuxia Medical Technology Co., Ltd., BSC-1000ⅡA2

Incubator: Shanghai Jinghong Experimental Equipment Co., Ltd., MJPS-250

Reagents and Materials

Strain: Malassezia furfur NBCC337308

Medium: Pityrosporum culture medium base Shandong Tuopu Biological Engineering Co., Ltd. Tween 40: Shandong Tuopu Biological Engineering Co., Ltd.

Glyceryl monooleate: Shandong Tuopu Bioengineering Co., Ltd.

Laboratory water: meet the quality requirements of GB 4789.28-2013 inspection medium and reagents

(2) Experimental steps

1) Sample action concentration 0.1%;

2) Add 0.1mL of Malassezia furfur suspension and mix thoroughly. After 20 minutes from the start of timing, dilute appropriately. Take 2-3 of the dilutions, put 1mL in two parallel plates, and then add the corresponding culture Mix the base and incubate at 30°C for 4 days. The above experiment was repeated three times, and the average number was taken, and a blank control was set at the same time;

3) Count the colonies after culturing at 30°C for 4 days and calculate the bacteriostatic rate. The results are shown in Table 1.

In the formula: Ⅰ—the average number of colonies in the control group; Ⅱ—the average number of colonies in the test group; the results are kept to two decimal places.

Table 1 Bacterial inhibition rate

2. Use test

The product of embodiment 1-embodiment 8 is joined in the mixture of water, surfactant according to mass 6.1%, wherein the content of water is 40%, surfactant is sodium lauryl sulfate, and content is 59%, mixing and stirring Get the shampoo evenly.

Randomly select 160 people with healthy scalp from 30 to 35 years old, and randomly divide them into 8 groups on average. Each group tests a product of the embodiment, uses it once every two days in the evening, and uses it for 3 minutes each time, and the water temperature is 42°C; after using it continuously for 3 times, the The feelings after use are counted, and the statistical results are shown in Table 2.

Questionnaire:

1. Before using the product, I feel that it has the effect of relieving itching

○5 points indicate complete agreement.

○4 points indicate that I agree

○3 points means neither agree nor agree

○2 points indicate that they disagree

○1 point means completely disagree

2. Before using the product, I feel that it has a soothing effect on the scalp

○ 5 points means fully agree

○4 points indicate that I agree

○3 points means neither agree nor agree (i.e. general)

○2 points indicate that they disagree

○1 point means completely disagree

Table 2 Use test statistics results

3. The product of Example 1 is added to the mixture of water and surfactant according to the mass fraction of 6.1%, wherein the content of water is 40%, the surfactant is sodium lauryl sulfate, and the content is 53.9%, mix and stir evenly Get shampoo. Select people with long-term dandruff problems to use for 2 weeks, the effect is shown in Figure 1. After 2 weeks of use, no dandruff was found on the scalp, indicating that the anti-dandruff composition of the present invention has a very good anti-dandruff effect.

4. Soothing test

Hyaluronidase activity experiment: Related literature shows that hyaluronic acid exists widely in skin and other tissues, and the relationship between hyaluronic acid and skin is very close. Macromolecular hyaluronic acid can inhibit inflammatory Discomfort can provide relief. Macromolecular hyaluronic acid will be degraded under the action of hyaluronidase, resulting in a decrease in the content of macromolecular hyaluronic acid. Decomposition produces the degradation product N-acetylglucosamine. Determination of N-acetylglucosamine in the reaction system can indirectly reflect the activity of hyaluronidase. In vitro hyaluronidase activity inhibition test was used to evaluate the anti-allergic activity of the sample, and the inhibition of hyaluronidase activity can reduce the degradation of hyaluronic acid and reduce skin sensitivity.

Detection method: mix the compositions of Examples 1-8 with the hyaluronidase solution respectively, the concentration of the composition in the solution is 6.1w%, shake and mix and incubate at 37° C. for 10 minutes. Add hyaluronate solution and incubate at 37°C for 45min. Add BSA solution and continue to incubate for 10 min, detect OD _600nm value, and calculate hyaluronidase inhibition rate. See Table 1.

Table 3 hyaluronidase activity inhibition rate

group Inhibition rate% Example 1 100 Example 2 86 Example 3 82 Example 4 79 Example 5 73 Example 6 81 Example 7 77 Example 8 83

It can be seen from the examples that the soothing effect of the composition of the present invention is better.

5. Application in the field of skin care products.

The composition obtained in Examples 1-8 was added to the face cream according to the addition amount of 5wt%, and the efficacy test was carried out.

Among them, the formula of the final face cream is as follows:

Water 65.15%, Compositions of Examples 1-8 5%, Caprylmethicone 3%, Isononyl Isononanoate 3%, Glycerin 2%, 1,2-Hexanediol 2%, Beet Alkali 0.8%, proline 0.1%, glyceryl stearate 1.05%, polydimethylsiloxane 1.35%, mannose 0.019%, cetearyl alcohol 1%, water balance.

Wherein, the soothing effect of embodiment 1 is the best, specifically see Fig. 2.

The above description is a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, these improvements and modifications It should also be regarded as the protection scope of the present invention.

Claims (10)

1. An anti-dandruff, antipruritic and soothing composition, which is characterized by comprising lophatherum gracile extract, andrographis paniculata extract and cortex phellodendri extract, wherein the mass ratio of the lophatherum gracile extract to the andrographis paniculata extract to the cortex phellodendri extract is 1: (1-6): (1-20).

2. The anti-dandruff, antipruritic, and soothing composition of claim 2 wherein the mass ratio of lophatherum gracile extract, andrographis paniculata extract, and phellodendron bark extract is 1: (1-3): (2-5).

3. The anti-dandruff, antipruritic, and soothing composition of claim 2 wherein the mass ratio of lophatherum gracile extract, andrographis paniculata extract, and phellodendron bark extract is 1:1.5:3.6.

4. a process for the preparation of an anti-dandruff, antipruritic, soothing composition as claimed in any of claims 1-3, comprising the steps of:

(1) Extraction of

Preparing lophatherum gracile extract: grinding the dried lophatherum gracile, and heating and extracting by using mixed alcohol consisting of methanol and ethanol, wherein the volume ratio of the methanol to the ethanol is 2-5: 1, obtaining an extracting solution, concentrating at normal pressure, and drying to obtain a lophatherum gracile extract;

preparation of Andrographis paniculata leaf extract: grinding herba Andrographitis leaves, soaking in distilled water for 30-60 min, preheating at 40-50deg.C for 1-2 hr, heating to boil and maintaining for 2-3 hr to obtain extractive solution, concentrating, and drying to obtain herba Andrographitis leaf extract;

preparation of cortex Phellodendri bark extract: grinding cortex phellodendri, adding distilled water A, stirring, soaking for 1-2 h, heating to boil, maintaining for 60-90 min, and filtering to obtain residue and primary extract; adding distilled water B into the residues, heating to boil, maintaining for 20-30 min, and filtering to obtain re-extract; mixing the primary extract and the secondary extract, concentrating, and oven drying to obtain cortex Phellodendri bark extract;

(2) Fermentation

Mixing herba Lophatheri extract, herba Andrographitis extract and cortex Phellodendri extract to obtain a mixture, adding carbon source and the mixture into sterile water, adjusting pH to 6.9-7.1 with PBS buffer solution, and sterilizing with ultraviolet to obtain fermentation medium; inoculating bacillus subtilis and Acetobacter pasteurii into a Gao's culture medium respectively, and culturing for 12-18 h to obtain strain seed liquid respectively; inoculating the strain seed liquid into a fermentation culture medium, fermenting and culturing for 35-45 h at 35-38 ℃, filtering, and freeze-drying the fermentation liquid to obtain the composition for removing dandruff, relieving itching and relieving.

5. The preparation method according to claim 4, wherein the weight ratio of the lophatherum gracile to the mixed alcohol is 1: 8-15, and the extraction time is 30-60 minutes.

6. The method according to claim 5, wherein the volume ratio of methanol to ethanol is 3:1, the weight ratio of the lophatherum gracile to the mixed alcohol is 1:10, the extraction time was 40 minutes.

7. The preparation method according to claim 4, wherein the volume ratio of the andrographis paniculata leaves to the distilled water is 1:10 to 12; the volume ratio of the cortex phellodendri to distilled water A is 1: 15-20; the volume ratio of the dregs to distilled water B is 1:10 to 12.

8. The method according to claim 4, wherein the carbon source is one or more selected from glucose, fructose, mannose, galactose; the mass ratio of the carbon source to the mixture to the sterile water is (10-20) to (10-15) to 75; the acetobacter pasteurii is aerobically cultured at a temperature of between 35 and 38 ℃, with an oxygen content of between 18 and 22 percent and a humidity of between 70 and 75 percent; the bacillus subtilis is cultivated in an anaerobic mode, the temperature is 35-38 ℃, and the humidity is 70-75%; the inoculation amount of the acetobacter pasteurii is 2-5%; the inoculation amount of the bacillus subtilis is 2-5%.

9. Use of an antidandruff, antipruritic, soothing composition according to any of claims 1 to 3 in shampoos, skin care products.

10. The use according to claim 9, wherein the antidandruff, antipruritic, soothing composition is added in an amount of 0.1% to 10% by weight.

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