CN114591380B - Method for extracting adenosine cyclophosphate - Google Patents
Method for extracting adenosine cyclophosphate Download PDFInfo
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- CN114591380B CN114591380B CN202210286621.5A CN202210286621A CN114591380B CN 114591380 B CN114591380 B CN 114591380B CN 202210286621 A CN202210286621 A CN 202210286621A CN 114591380 B CN114591380 B CN 114591380B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
- C07H19/213—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids containing cyclic phosphate
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Abstract
The application discloses a method for extracting adenosine cyclophosphate, which belongs to the field of adenosine cyclophosphate extraction, and adopts the scheme that the method comprises the steps of selecting Chinese dates, soaking the Chinese dates in hot water, drying, preparing jujube paste and extracting the adenosine cyclophosphate, so that the adenosine cyclophosphate can be extracted from the Chinese dates rich in the adenosine cyclophosphate, the use of chemical agents is reduced in the production process, the final product color of the adenosine cyclophosphate is not easily influenced, the Chinese dates are boiled by utilizing a special container in the treatment process of the Chinese dates, low-temperature distilled water is conveyed into the container main body of the Chinese dates through a connecting through hole, the water adding work is automatically finished, the temperature difference of cold water to be gradually increased in the rising process of the cold water is greatly reduced, the damage to the Chinese dates is reduced, the boiling effect is increased, the influence of metering difference caused by manual water adding on the Chinese dates is reduced, and the subsequent extraction effect is increased.
Description
Technical Field
The application relates to the field of adenosine cyclophosphate extraction, in particular to a method for extracting adenosine cyclophosphate.
Background
Ferulic acid (sodium ferulate) has effects of resisting platelet aggregation, inhibiting platelet 5-hydroxytryptamine release, inhibiting thromboxane a2 (txa 2) generation, enhancing prostaglandin activity, relieving pain, and relieving vasospasm. Is a basic raw material for producing medicines for treating cardiovascular and cerebrovascular diseases, leucopenia and other symptoms. Such as Xinxuekang, limai capsule, taitai oral liquid, etc., which can play the roles of building body and protecting skin in human body at the same time, and the research shows that: ferulic Acid (FA) can regulate PDE expression and activity, thereby affecting various metabolism of organisms, phosphodiesterase (PDE) plays a great role in learning and memory processes, and corresponding inhibitors can improve learning and memory disorders caused by degenerative diseases of the central nervous system by regulating the content of intracellular cyclic adenosine monophosphate (cyclic adenosine monophosphate, camp), namely, the cyclic adenosine monophosphate is controlled to achieve the beneficial effects when ferulic acid is taken up finally.
Thus, cyclic adenosine has the reputation of "first messenger of life", has the effects of improving sleep disorder, cardiovascular function, brain function, blood circulation, preventing cancer occurrence, protecting liver function, enhancing organism immunity, hematopoietic function, preventing allergic diseases, and filling qi and blood, and salon finds cyclic adenosine monophosphate, and more so, in 1971, the professor candel at university of columbia in the united states plays a very important role in brain cell repair by studying cyclic adenosine, and thus, the prize of nobel in 2000 is awarded.
The existing production method of the adenosine cyclophosphate is mainly prepared by a chemical synthesis method, for example, the adenosine cyclophosphate is prepared by taking the adenosine cyclophosphate as a raw material, and is subjected to one-step cyclization under the action of acetic anhydride to obtain the acetylated adenosine cyclophosphate, and then the acetylated adenosine cyclophosphate is subjected to alkaline hydrolysis, so that the adenosine cyclophosphate can be obtained, but the production cost is relatively high, and a small amount of chemical raw materials are easily mixed in the finally extracted adenosine cyclophosphate in the production work, so that the color of a finished product of the adenosine cyclophosphate is influenced.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems existing in the prior art, the application aims to provide the extraction method of the adenosine cyclophosphate, which can realize the extraction of the adenosine cyclophosphate from the jujube rich in the adenosine cyclophosphate, reduce the use of chemical agents in the production process and not easily influence the final finished product color of the adenosine cyclophosphate.
2. Technical proposal
In order to solve the problems, the application adopts the following technical scheme.
The method for extracting the adenosine cyclophosphate mainly comprises the following steps of:
s1, screening picked Chinese dates, namely screening 8-degree-of-ripe and full-grain cyan Chinese dates, wherein the Chinese dates are preferably Yiwu Chinese dates;
s2, soaking with hot water, namely, quickly scalding the screened Chinese dates with boiling water for 2 seconds, stewing the scalded Chinese dates in a closed thermal container for 2 hours, taking out the stewed Chinese dates, spreading the stewed Chinese dates on a straw mat, exposing the stewed Chinese dates to sunlight until the Chinese date skins are colored red, twisting the Chinese date skins into wrinkled Chinese dates, cleaning the Chinese dates, boiling the Chinese dates with boiling water, placing the Chinese dates in a container with boiling water, boiling the boiling water, adding cold water, lightly turning over the boiled water, repeating the boiling for 15 minutes until the Chinese dates turn soft, touching the Chinese date pits, and exposing the boiled Chinese dates to sunlight for 4 hours;
s3, drying, namely spreading the date subjected to hot water soaking on a date kang, covering the date surface with a double silk gunny bag, and drying for 5 days continuously, wherein the date is turned once every 2 hours;
s4, preparing jujube paste, namely selecting 50kg of jujube to break into jujube powder, adding 50kg of glutinous rice and 100kg of clear water to stir uniformly to form a fermentation matrix, taking out the fermentation matrix after steaming by a steamer, cooling thoroughly, adding yeast to stir, and sealing for fermentation for 30 days;
s5, extracting the adenosine cyclophosphate, and extracting liquid adenosine cyclophosphate and solid adenosine cyclophosphate by utilizing a biological enzyme catalytic reaction after fermentation parent is mature.
In step S2, vegetable oil is added into the cleaning water to increase the cleaning effect when the jujube is cleaned.
Further, step S2, in the hot water oozes bubble, in the boiling water boiling process, the container that holds boiling water includes the container main part, container main part 'S lower extreme fixedly connected with inlet tube, the inlet tube is inserted and is equipped with the sealed pipe with self assorted, the cutting has a plurality of connecting through-holes on the lateral wall of sealed pipe, a plurality of connecting through-holes intercommunication self are inside and external, the outside cover of connecting through-hole is equipped with extension spring, extension spring' S both ends respectively with inlet tube and connecting through-hole fixed connection, the upper end fixedly connected with magnetism hoop of inlet tube, the upper end of sealed pipe is selected for use waterproof electromagnet to make, fixedly connected with fan-shaped electrical heating board on the bottom plate of container main part, carry low temperature distilled water in to the container main part through connecting through-hole, accomplish the work of adding water automatically, and the cold water can rise gradually, reduce the difference in temperature that the boiling jujube receives, reduce the damage that causes the jujube, increase the boiling effect.
Further, the cross section of the inlet wall at the lower end of the sealing tube is an inclined plane, the top end of the inner wall of the sealing tube is an arc surface, abrasion caused by water flushing is reduced, and the service life of the sealing tube is prolonged.
Further, the water outlet of the water inlet pipe is higher than the bottom plate of the container main body, so that the Chinese date sundries generated in the boiling process are not easy to fix on the upper side of the sealing pipe when being deposited on the bottom plate of the container main body, and the normal work of the water inlet pipe and the sealing pipe is influenced.
Further, be equipped with self assorted separation sieve in the container main part, separate the sieve and suspend in the boiling water in the container main part for the big granule debris that drops and produce in the boiling process of separation jujube, be difficult for causing the influence to the normal work of inlet tube and the sealed tube of downside.
Further, the container main body is fixedly connected with a limiting table, the limiting table is positioned on the upper side of the separation sieve, and the limiting table has a limiting effect on the separation sieve, so that the separation sieve floats to the surface of boiling water in the container main body, and the boiled jujube is not easy to influence.
Further, the separation screen comprises a floating ring, and a net frame matched with the floating ring is fixedly connected in the floating ring, wherein the floating ring and the net frame are made of elastic materials, so that the floating ring and the net frame can be conveniently taken out of the container main body.
Further, the floating ring has a tendency of outward expansion, the net frame is woven by elastic materials, so that the stability of the floating ring and the net frame is improved, and the floating ring and the net frame are not easy to fail due to excessive deformation in wastewater.
Further, the lower extreme fixedly connected with of net frame catches the fibre cluster, catches the fibre cluster and includes a plurality of elastic fiber, and a plurality of elastic fiber all are three-dimensional heliciform, and adjacent elastic fiber twines together and forms three-dimensional space three-dimensional structure, can catch the debris that the jujube drops in boiling in-process, is convenient for follow-up unified clearance work.
3. Advantageous effects
Compared with the prior art, the application has the advantages that:
according to the scheme, the jujube is selected, and the preparation processes of screening, hot water soaking, drying, jujube paste preparation and adenosine cyclophosphate extraction can be carried out, so that the adenosine cyclophosphate can be extracted from the jujube rich in the adenosine cyclophosphate, the use of chemical agents is reduced in the production process, and the final finished product color of the adenosine cyclophosphate is not easily affected.
In the jujube processing process, the specially-made container is utilized for carrying out jujube boiling work, low-temperature distilled water is conveyed into the container main body through the connecting through hole, the water adding work is automatically completed, the temperature of cold water is gradually increased in the rising process, the temperature difference suffered by the boiled jujube is greatly reduced, the damage to the jujube is reduced, the boiling effect is improved, the influence of metering difference caused by manual water adding on the jujube boiling is reduced, the subsequent extraction effect is improved, meanwhile, the separating screen is arranged in the container main body, sundries falling off in the boiling process of the jujube can be captured, and the follow-up unified cleaning work is facilitated.
Drawings
FIG. 1 is a main flow chart of the method for extracting adenosine cyclophosphate of the present application;
FIG. 2 is a flowchart showing hot water soaking (hot red) in the adenosine cyclophosphate extraction method of the present application;
FIG. 3 is a schematic structural view of the boiling water boiling vessel of the present application;
fig. 4 is a front cross-sectional view of the boiling water boiling vessel of the present application;
FIG. 5 is a schematic diagram of the structure shown at A in FIG. 4;
FIG. 6 is a schematic diagram of the structure at B in FIG. 4;
fig. 7 is a schematic view showing a structural change during the operation of the boiling water boiling vessel of the present application;
FIG. 8 is a table 1 showing the concentration detection of the adenosine cyclophosphate in the fermentation mother substrate of the present application;
FIG. 9 is a table 2 showing the concentration detection of the adenosine cyclophosphate in the fermentation mother substrate of the present application;
FIG. 10 is a graph showing a fit of a calibration set of concentrations of adenosine cyclophosphate in the fermentation broths of the present application;
FIG. 11 is a table showing a comparative group of concentrations of adenosine cyclophosphate in fermentation broths according to the present application;
FIG. 12 is a chromatogram A of a fermentation mother cyclic adenosine monophosphate concentration test A-r001.D group of the present application;
FIG. 13 is a chromatogram B of a fermentation cylinder of the present application for the detection of adenosine cyclophosphate concentration in group A-r001. D;
FIG. 14 is a chromatogram A of a fermentation parent cyclic adenosine monophosphate concentration test B-r001.D group of the present application;
FIG. 15 is a chromatogram B of a fermentation mother cyclic adenosine monophosphate concentration test B-r001.D group of the present application;
FIG. 16 is a chromatogram A of a fermentation parent cyclic adenosine monophosphate concentration test C-r001.D group of the present application;
FIG. 17 is a chromatogram B of a fermentation parent cyclic adenosine monophosphate concentration test C-r001.D group of the present application;
FIG. 18 is a chromatogram A of a fermentation cylinder of the present application for the detection of adenosine cyclophosphate concentration in the S1-r001.D group;
FIG. 19 is a chromatogram B of the fermentation cylinder of the S1-r001.D group for detecting the concentration of adenosine cyclophosphate of the present application;
FIG. 20 is a chromatogram A of a fermentation parent cyclic adenosine monophosphate concentration test S2-r001.D group of the present application;
FIG. 21 is a chromatogram B of the fermentation cylinder of the S2-r001.D group for detecting the concentration of adenosine cyclophosphate of the present application;
FIG. 22 is a chromatogram A of the fermentation cylinder of the S3-r001.D group for detecting the concentration of adenosine cyclophosphate of the present application;
FIG. 23 is a chromatogram B of the fermentation cylinder of the S3-r001.D group for detecting the concentration of adenosine cyclophosphate of the present application;
FIG. 24 is a chromatogram A of a fermentation cylinder of the present application for detection of adenosine cyclophosphate concentration in the S4-r001.D group;
FIG. 25 is a chromatogram B of the fermentation cylinder of the present application for the detection of adenosine cyclophosphate concentration in the S4-r001.D group; .
The reference numerals in the figures illustrate:
1 a container main body, 2 a limiting table, 3 a separation sieve, 301 a floating ring, 302 a net frame and 303 a fiber cluster capturing 4 water inlet pipes, 5 a magnetic iron ring, 6 a sealing pipe, 7 a connecting through hole, 8 a tension spring and 9 fan-shaped electric heating plates.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application; it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present application are within the protection scope of the present application.
In the description of the present application, it should be noted that the positional or positional relationship indicated by the terms such as "upper", "lower", "inner", "outer", "top/bottom", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present application and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "configured to," "engaged with," "connected to," and the like are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of the element of the adapting model. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
Example 1:
referring to fig. 1-2, a method for extracting adenosine cyclophosphate mainly comprises the following steps:
s1, screening picked Chinese dates, namely screening 8-degree-of-ripe and full-grain cyan Chinese dates, wherein the Chinese dates are preferably Yiwu Chinese dates;
s2, soaking with hot water, namely, quickly scalding the screened Chinese dates with boiling water for 2 seconds, stewing the scalded Chinese dates in a closed thermal container for 2 hours, taking out the stewed Chinese dates, spreading the stewed Chinese dates on a straw mat, exposing the stewed Chinese dates to sunlight until the Chinese date skins are colored red, twisting the Chinese date skins into wrinkled Chinese dates, cleaning the Chinese dates, boiling the Chinese dates with boiling water, placing the Chinese dates in a container with boiling water, boiling the boiling water, adding cold water, lightly turning over the boiled water, repeating the boiling for 15 minutes until the Chinese dates turn soft, touching the Chinese date pits, and exposing the boiled Chinese dates to sunlight for 4 hours;
s3, drying, namely spreading the date subjected to hot water soaking on a date kang, covering the date surface with a double silk gunny bag, and drying for 5 days continuously, wherein the date is turned once every 2 hours;
s4, preparing jujube paste, namely selecting 50kg of jujube to break into jujube powder, adding 50kg of glutinous rice and 100kg of clear water to stir uniformly to form a fermentation matrix, taking out the fermentation matrix after steaming by a steamer, cooling thoroughly, adding yeast to stir, and sealing for fermentation for 30 days;
s5, extracting the adenosine cyclophosphate, and extracting liquid adenosine cyclophosphate and solid adenosine cyclophosphate by utilizing a biological enzyme catalytic reaction after fermentation parent is mature.
Referring to FIGS. 8-25, for a table and a chromatogram of the measurement of the concentration of the adenosine cyclophosphate of the fermentation mother liquor of the present embodiment, the normal sample components are three groups A-r001.D, B-r001.D and C-r001.D, and the same test method is used to set up a calibration group 4: s1-r001d, S2-r001d, S3-r001d and S4-r001d, wherein the calibration group is used for checking whether the detection method is accurate or not, and is a liquid with a concentration approximately equal to the difference sequence which is configured in advance, a coordinate system is established for the sample adenosine cyclophosphate concentration and response value data of the calibration group, a straight line can be obtained by fitting, the slope of the straight line is about 40, and the obvious linear relation exists between the adenosine cyclophosphate concentration and the response value in the measurement data of the calibration group, namely, the adenosine cyclophosphate concentration data obtained in the detection method is true and reliable.
As can be seen from the chromatogram of the final product, the concentration of the final fermentation parent cyclic adenosine monophosphate produced by the scheme is 250-300mg/L.
Finally, the technique of extracting the adenosine cyclophosphate by using the biological enzyme refers to that the biological enzyme is utilized to decompose the jujube cell tissues, release the adenosine cyclophosphate and then carry out the extraction work, which is a known technology of the person skilled in the art, and is not disclosed in detail in the application.
In the step S2, during soaking with hot water, vegetable oil is added into the cleaning water when the jujube is cleaned, so that the cleaning effect is improved.
Referring to fig. 3-7, in step S2, in the hot water soaking, in the boiling water boiling process, the container containing boiling water includes a container body 1, a water inlet pipe 4 is fixedly connected to the lower end of the container body 1, a sealing pipe 6 matched with the water inlet pipe 4 is inserted in the water inlet pipe, a plurality of connecting through holes 7 are cut on the side wall of the sealing pipe 6, the plurality of connecting through holes 7 are communicated with the inside and the outside of the water inlet pipe, a tension spring 8 is sleeved on the outer side of the connecting through holes 7, two ends of the tension spring 8 are fixedly connected with the water inlet pipe 4 and the connecting through holes 7 respectively, a magnetic iron ring 5 is fixedly connected to the upper end of the water inlet pipe 4, a waterproof electromagnet is used for sorting the upper end of the sealing pipe 6, a fan-shaped electric heating plate 9 is fixedly connected to the bottom plate of the container body 1, wherein the water inlet pipe 4 is communicated with a water delivery pipe, distilled water with the temperature of 10 ℃ can be delivered to the container body 1 through the water inlet pipe 4, in the scheme, through the adsorption action between the magnetic iron ring 5 and the sealing tube 6 and the balance between the water pressure in the container main body 1 and the tensile force and water delivery pressure generated by the deformation of the tension spring 8, the automatic water addition in the container main body 1 is realized, when boiling water is boiled normally, the liquid level in the container main body 1 is positioned at a high position, the hydraulic pressure born by the sealing tube 6 is larger, at the moment, under the combined action of the adsorption action between the magnetic iron ring 5 and the sealing tube 6 and the water pressure in the container main body 1, the sealing tube 6 is positioned in the water inlet tube 4, the water delivery work can not be carried out, and when the liquid level in the container main body 1 is positioned at a low position, the hydraulic pressure born by the sealing tube 6 is reduced, at the moment, under the combined action of the tensile force generated by the deformation of the tension spring 8 and the water delivery pressure, the sealing tube 6 is jacked up, and low-temperature distilled water is delivered into the container main body 1 through the connecting through the through hole 7, the water adding work is automatically completed, the temperature of the cold water is gradually increased in the rising process, the temperature difference of the boiled dates is greatly reduced, the damage to the dates is reduced, and the boiling effect is improved.
In particular, in the above description, the gravity of the sealing tube 6 is not considered in the stress analysis, so that the gravity applied to the sealing tube 6 is fixed, and can be regarded as a system fixing parameter, and the quantitative analysis is not performed, and in addition, since the magnitude of the adsorption force between the magnetic iron ring 5 and the sealing tube 6 is related to the position distance between the two, after the liquid level in the container main body 1 reaches the set position, the magnetic iron ring 5 and the sealing tube 6 are adsorbed, the magnetic iron ring 5 and the sealing tube are not separated again due to the slight change of the liquid level in the container main body 1, so that the water injection work is not easy to frequent. And the distilled water flow in the container body 1 is mainly generated by vaporization of boiling water.
The cross-section of the lower extreme entry wall of seal tube 6 is the inclined plane, and seal tube 6 inner wall top is the cambered surface, reduces the wearing and tearing that water erodees produced, increases seal tube 6's life, and the delivery port department of inlet tube 4 is higher than the bottom plate of container main part 1 for the produced jujube debris of boiling in-process deposits the upside at seal tube 6 when the container main part 1 bottom plate position, causes the influence to the normal work of inlet tube 4 and seal tube 6.
Be equipped with self assorted separating screen 3 in the container main part 1, separating screen 3 is suspended in the boiling water in the container main part 1, be used for screening out big granule debris that drops and produce in the jujube boiling process, be difficult for causing the influence to the normal work of inlet tube 4 and sealed tube 6 of downside, container main part 1 fixedly connected with spacing platform 2, spacing platform 2 is located the upside of separating screen 3, spacing platform 2 has spacing effect to separating screen 3, make separating screen 3 float to the surface of boiling water in the container main part 1, be difficult for causing the influence to the jujube of boiling, separating screen 3 includes floating ring 301, floating ring 301 internal fixation has the net frame 302 with self assorted, wherein floating ring 301 and net frame 302 are made for elastic material, be convenient for take out floating ring 301 and net frame 302 from container main part 1, floating ring 301 has the trend of outwards expanding, net frame 302 is woven with elastic material, increase floating ring 301 and net frame 302's stability, be difficult for losing efficacy because of taking place the excessive deformation in the waste water, the lower extreme fixedly connected with of net frame 302 catches fibre 303, it includes a plurality of adjacent fibre to catch fibre, it is three-dimensional elasticity is formed in the three-dimensional fibre is continuous to have a plurality of adjacent fibre to take out, three-dimensional fibre is three-dimensional and is formed in the three-dimensional and is convenient for the three-dimensional to have a three-dimensional fibre is adjacent to the fibre to be a three-dimensional and is adjacent to the fibre is adjacent to the adjacent to form a plurality of fibre to take out in a three-dimensional fibre.
According to the scheme, the jujube is selected, and the preparation processes of screening, hot water soaking, drying, jujube paste preparation and adenosine cyclophosphate extraction can be carried out, so that the adenosine cyclophosphate can be extracted from the jujube rich in the adenosine cyclophosphate, the use of chemical agents is reduced in the production process, and the final finished product color of the adenosine cyclophosphate is not easily affected.
The prepared adenosine cyclophosphate product is commonly used for oral liquid, paste prescription and soup making.
In the jujube processing process, the specially-made container is utilized for carrying out jujube boiling work, low-temperature distilled water is conveyed into the container main body 1 through the connecting through hole 7, the water adding work is automatically completed, the temperature of cold water is gradually increased in the rising process, the temperature difference suffered by the boiled jujube is greatly reduced, the damage to the jujube is reduced, the boiling effect is improved, the influence of metering difference caused by manual water adding on the jujube boiling is reduced, the subsequent extraction effect is improved, meanwhile, the separating screen 3 is arranged in the container main body 1, sundries falling off in the boiling process of the jujube can be captured, and the follow-up unified cleaning work is facilitated.
The above description is only of the preferred embodiments of the present application; the scope of the application is not limited in this respect. Any person skilled in the art, within the technical scope of the present disclosure, may apply to the present application, and the technical solution and the improvement thereof are all covered by the protection scope of the present application.
Claims (9)
1. A method for extracting adenosine cyclophosphate is characterized in that: mainly comprises the following steps:
s1, screening picked Chinese dates, namely screening 8-degree-of-ripe and full-grain cyan Chinese dates, wherein the Chinese dates are preferably Yiwu Chinese dates;
s2, soaking with hot water, namely, scalding the screened Chinese dates quickly with boiling water for 2 seconds, stewing the scalded Chinese dates in a closed thermal insulation container for 2 hours, taking out the stewed Chinese dates, spreading the stewed Chinese dates on a straw mat, exposing the stewed Chinese dates to sunlight until the Chinese date skins are colored red, twisting the Chinese date skins into wrinkled Chinese dates, cleaning the Chinese dates, boiling the Chinese dates with boiling water, placing the Chinese dates in a container filled with boiling water, boiling the container filled with boiling water, wherein the container comprises a container main body (1), the lower end of the container main body (1) is fixedly connected with a water inlet pipe (4), inserting a sealing pipe (6) matched with the water inlet pipe (4), the side wall of the sealing tube (6) is provided with a plurality of connecting through holes (7), the connecting through holes (7) are communicated with the inside and the outside, an extension spring (8) is sleeved outside the connecting through holes (7), two ends of the extension spring (8) are fixedly connected with a water inlet tube (4) and the connecting through holes (7) respectively, the upper end of the water inlet tube (4) is fixedly connected with a magnetic iron ring (5), the upper end of the sealing tube (6) is made of a waterproof electromagnet, a fan-shaped electric heating plate (9) is fixedly connected to the bottom plate of the container main body (1), cold water is added after boiling, the boiling is repeated for 3 times, the boiling time is 15min, until a date body is soft, the date pit can be touched, and then the boiled date is exposed for 4h;
s3, drying, namely spreading the date subjected to hot water soaking on a date kang, covering the date surface with a double silk gunny bag, and drying for 5 days continuously, wherein the date is turned once every 2 hours;
s4, preparing jujube paste, namely selecting 50kg of jujube to break into jujube powder, adding 50kg of glutinous rice and 100kg of clear water to stir uniformly to form a fermentation matrix, taking out the fermentation matrix after steaming by a steamer, cooling thoroughly, adding yeast to stir, and sealing for fermentation for 30 days;
s5, extracting the adenosine cyclophosphate, and extracting liquid adenosine cyclophosphate and solid adenosine cyclophosphate by utilizing a biological enzyme catalytic reaction after fermentation parent is mature.
2. The method for extracting adenosine cyclophosphate according to claim 1, wherein: in the step S2, during soaking with hot water, vegetable oil is added into the cleaning water when the jujube is cleaned.
3. The method for extracting adenosine cyclophosphate according to claim 1, wherein: the cross section of the inlet wall at the lower end of the sealing tube (6) is an inclined plane, and the top end of the inner wall of the sealing tube (6) is an arc surface.
4. The method for extracting adenosine cyclophosphate according to claim 1, wherein: the water outlet of the water inlet pipe (4) is higher than the bottom plate of the container main body (1).
5. The method for extracting adenosine cyclophosphate according to claim 1, wherein: the container is characterized in that a separation sieve (3) matched with the container body (1) is arranged in the container body (1), and the separation sieve (3) is suspended in boiling water in the container body (1).
6. The method for extracting adenosine cyclophosphate according to claim 5, wherein: the container is characterized in that the container body (1) is fixedly connected with a limiting table (2), and the limiting table (2) is positioned on the upper side of the separation sieve (3).
7. The method for extracting adenosine cyclophosphate according to claim 5, wherein: the separation sieve (3) comprises a floating ring (301), and a net frame (302) matched with the floating ring (301) is fixedly connected in the floating ring.
8. The method for extracting adenosine cyclophosphate according to claim 7, wherein: the floating ring (301) has a tendency to expand outwards, and the net frame (302) is woven from an elastic material.
9. The method for extracting adenosine cyclophosphate according to claim 7, wherein: the lower extreme fixedly connected with of screen frame (302) catches fibre cluster (303), catch fibre cluster (303) include a plurality of elastic fiber, a plurality of elastic fiber all is three-dimensional heliciform, adjacent elastic fiber twines together and forms three-dimensional space three-dimensional structure.
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| CN101525363A (en) * | 2009-04-24 | 2009-09-09 | 天津科技大学 | Method for extracting cyclic adenosine monophosphate (cAMP) from red jujubes |
| CN202620784U (en) * | 2012-06-21 | 2012-12-26 | 新疆刀朗枣业有限公司 | Extracting device for producing cyclic adenosine monophosphate and flavone of red dates |
| CN104783089A (en) * | 2015-03-27 | 2015-07-22 | 天津市食品研究所有限公司 | Preparation method of jujube extract |
| CN109181966A (en) * | 2018-10-31 | 2019-01-11 | 王英俊 | A kind of south jujube health preserving wine and preparation method thereof |
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