CN114591380A - Extraction method of adenosine cyclophosphate - Google Patents

Abstract

The invention discloses a method for extracting cyclophosphate adenosine, which belongs to the field of cyclophosphate adenosine extraction, and a method for extracting cyclophosphate adenosine. In this scheme, jujube is selected, and after screening, hot water infiltration, drying, and jujube puree The preparation process of preparation and cyclophosphate extraction can realize the extraction of cyclophosphate from jujube rich in cyclophosphate, reduce the use of chemical agents in the production process, and not easily affect the final product of cyclophosphate. At the same time, in the process of jujube processing, a special container is used to cook the jujube, and the low-temperature distilled water is delivered to the main body of the jujube-made container through the connecting through hole, and the water filling work is automatically completed, while the cold water is in the process of rising. It will gradually heat up, greatly reduce the temperature difference for cooking jujube, reduce the damage to the jujube, increase the cooking effect, reduce the influence of the measurement difference caused by artificial water addition on the jujube cooking, and increase the subsequent extraction effect. .

Description

A kind of extraction method of cycloadenosine

technical field

The present invention relates to the extraction field of cyclic adenosine monophosphate, and more particularly, to a method for extracting cyclic adenosine monophosphate.

Background technique

Ferulic acid (sodium ferulate) has anti-platelet aggregation, inhibits platelet serotonin release, inhibits platelet thromboxane a2 (txa2) production, enhances prostaglandin activity, analgesia, and relieves vasospasm. It is the basic raw material for the production of medicines for the treatment of cardiovascular and cerebrovascular diseases and leukopenia. Such as Xinxuekang, Limai Capsule, Mrs. Oral Liquid, etc., it can also play a role in bodybuilding and skin protection in the human body, and research has shown that ferulic acid (FA) can regulate the expression and activity of PDE , thereby affecting various metabolisms of organisms, phosphodiesterase (Phosphodiesterase, PDE) plays a great role in the process of learning and memory, and its corresponding inhibitor can regulate intracellular cyclic adenosine monophosphate (cyclic adenosine monophosphate, cAMP) content to improve learning and memory impairment caused by degenerative diseases of the central nervous system, that is, the above beneficial effects can be achieved by controlling cyclic adenosine monophosphate in the final intake of ferulic acid.

Therefore, cyclophosphate has the reputation of "the first messenger of life", which can improve sleep disorders, cardiovascular function, brain function, blood circulation, prevent cancer, protect liver function, enhance immunity, hematopoietic function, and prevent allergic diseases. , The effect of filling qi and blood, Sutherland discovered cyclic adenosine monophosphate, and was awarded the Nobel Prize in Physiology/Medicine in 1971. Professor Kandel of Columbia University has a very important role in brain cell repair by researching cyclic adenosine monophosphate. , so won the 2000 Nobel Prize in Medicine.

The existing production method of cyclophosphate adenosine is mainly prepared by a chemical synthesis method, such as using adenylic acid as a raw material, in the next step of the action of acetic anhydride to obtain acetylated adenosine cyclophosphate by cyclization, and then through alkaline hydrolysis can be obtained. Cyclophosphate, but its production cost is relatively high, and it is easy to mix a small amount of chemical raw materials into the final extracted cyclophosphate in the production work, which affects the finished product quality of cyclophosphate.

SUMMARY OF THE INVENTION

1. Technical problems to be solved

In view of the problems existing in the prior art, the purpose of the present invention is to provide a method for extracting adenosine cyclophosphate, which can realize the extraction of adenosine cyclophosphate from jujubes rich in cyclophosphate, and reduce chemical production in the production process. The use of the drug will not easily affect the final product quality of cyclophosphate.

2. Technical solutions

In order to solve the above problems, the present invention adopts the following technical solutions.

A method for extracting cyclic adenosine, which mainly comprises the following steps:

S1, jujube screening, the picked jujubes are screened, select 8 minutes of ripe, cyan jujubes with full particles, wherein the jujubes are preferably Yiwu jujubes;

S2. Bubble with hot water, quickly scald the selected jujubes in boiling water, the scalding time is 2 seconds, place the scalded jujubes in an airtight thermal container and simmer for 2 hours, and then simmer the simmered jujubes for 2 hours. Take out the jujube and lay it flat on the straw mat, and expose it to the sun until the jujube skin turns red, and the jujube skin is twisted into a wrinkled skin. After cleaning the jujube, it is boiled in boiling water, Put it in a container filled with boiling water and cook, add cold water after the boiling water boils and turn it gently, repeat 3 times, the cooking time is 15min, until the body is soft and can touch the jujube core, and then put the cooked jujube Exposure to the sun for 4 hours;

S3, drying, laying the jujube soaked in hot water on the Kang Kang, covering the surface of the jujube with a double-silk sack, and baking for 5 consecutive days, turning it every 2 hours;

S4, the preparation of jujube paste, select 50kg of jujube and smash it to form a powder, then add 50kg of glutinous rice and 100kg of clean water and stir evenly to form a yeast body, steam it in a steamer, take it out and cool it, add yeast and stir and then close the fermentation for 30 days ;

S5, extraction of cyclic adenosine monophosphate, after the yeast body is fermented and matured, the liquid cyclic adenosine monophosphate and the solid cyclic adenosine monophosphate are extracted by catalytic reaction of biological enzymes.

Further, in step S2, in the hot water bubbling, when cleaning the jujube, vegetable oil is added to the cleaning water to increase the cleaning effect.

Further, in step S2, in the hot water bubbling, during the boiling water boiling process, the container containing the boiling water includes a container body, the lower end of the container body is fixedly connected with a water inlet pipe, and the water inlet pipe is inserted with a self-matching sealing pipe, A plurality of connecting through holes are drilled on the side wall of the sealing pipe, and the plurality of connecting through holes communicate with the inside and the outside world. The outer side of the connecting through hole is sleeved with a tension spring, and the two ends of the tension spring are respectively connected with the water inlet pipe and the connection. The upper end of the water inlet pipe is fixedly connected with a magnetic iron ring, and the upper end of the sealing pipe is made of waterproof electromagnets. The low-temperature distilled water automatically completes the water filling work, and the cold water will gradually heat up during the rising process, which greatly reduces the temperature difference of the jujube cooking, reduces the damage to the jujube, and increases the cooking effect.

Further, the cross section of the inlet wall of the lower end of the sealing tube is an inclined surface, and the top end of the inner wall of the sealing tube is an arc surface, which reduces the wear caused by water scouring and increases the service life of the sealing tube.

Further, the water outlet of the water inlet pipe is higher than the bottom plate of the container body, so that the jujube debris generated during the cooking process is not easy to be fixed on the upper side of the sealing pipe when it settles on the bottom plate of the container body. normal work is affected.

Further, the main body of the container is provided with a self-matching separation screen, and the separation screen is suspended in the boiling water in the main body of the container to screen out the large particles of debris that fall off during the cooking process of the jujube, and it is not easy to enter the lower side. The normal operation of water pipes and sealing pipes is affected.

Further, the container main body is fixedly connected with a limit table, the limit table is located on the upper side of the partition screen, and the limit table has a limiting effect on the partition screen, so that the partition screen floats to the surface of the boiling water in the container main body, which is not easy to cook large. Jujube affects.

Further, the separating screen includes a floating ring, and the floating ring is fixedly connected with a mesh frame matching itself, wherein the floating ring and the mesh frame are made of elastic materials, so that the floating ring and the mesh frame can be easily taken out from the container body.

Further, the floating ring has a tendency to expand outward, and the mesh frame is woven with elastic materials, which increases the stability of the floating ring and the mesh frame, and is not easy to fail due to excessive deformation in wastewater.

Further, the lower end of the mesh frame is fixedly connected with a capturing fiber cluster, and the capturing fiber cluster includes a plurality of elastic fibers, and the multiple elastic fibers are all in a three-dimensional spiral shape. The debris that falls off during the cooking process is captured, which is convenient for subsequent unified cleaning.

3. Beneficial effects

Compared with the prior art, the advantages of the present invention are:

In this scheme, jujube is selected, and through the preparation process of screening, hot water soaking, drying, jujube paste preparation and adenosine cyclophosphate extraction, adenosine cyclophosphate can be extracted from jujube rich in cyclophosphate. The use of chemical agents is reduced in the process, and the final product color of cyclophosphate is not easily affected.

In the process of jujube processing, a special container is used to cook jujubes, and low-temperature distilled water is sent to the main body of the container through the connecting through holes, and the water filling work is automatically completed. The temperature difference received by the jujube reduces the damage to the jujube, increases the cooking effect, reduces the influence of the measurement difference caused by artificial water addition on the jujube cooking, and increases the subsequent extraction effect. The sieve can capture the sundries that fall off during the cooking process of jujube, which is convenient for subsequent unified cleaning.

Description of drawings

Fig. 1 is the main flow chart of the cyclophosphate adenosine extraction method of the present invention;

Fig. 2 is the concrete flow chart of hot water bubbling (hot red) in the adenosine cyclophosphate extraction method of the present invention;

Fig. 3 is the structural representation of the boiling water boiling container of the present invention;

Fig. 4 is the front sectional view of the boiling water cooking container of the present invention;

Fig. 5 is the structural representation at A place in Fig. 4;

Fig. 6 is the structural representation at place B in Fig. 4;

7 is a schematic diagram of structural changes in the working process of the boiling water cooking container of the present invention;

Fig. 8 is the detection table 1 of the concentration of cyclic adenosine monophosphate in yeast of the present invention;

Fig. 9 is the detection table 2 of the concentration of cyclic adenosine monophosphate in yeast of the present invention;

Fig. 10 is the fitting diagram of the detection and proofreading group of yeast cycloadenosine cyclophosphate concentration of the present invention;

Figure 11 is the detection table of the yeast cycloadenosine cyclophosphate concentration detection proof group of the present invention;

Fig. 12 is the chromatogram A of group A-r001.d for detecting the concentration of cyclic adenosine monophosphate in yeast of the present invention;

Fig. 13 is the chromatogram B of the A-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Figure 14 is the chromatogram A of the detection of the concentration of cyclic adenosine monophosphate in yeast of the present invention of group B-r001.d;

Fig. 15 is the chromatogram B of the B-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Fig. 16 is the chromatogram A of C-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Fig. 17 is the chromatogram B of C-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Figure 18 is the chromatogram A of the S1-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Figure 19 is the chromatogram B of the S1-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Figure 20 is the chromatogram A of the S2-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Figure 21 is the chromatogram B of the S2-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Figure 22 is the chromatogram A of the S3-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Figure 23 is the chromatogram B of the S3-r001.d group of yeast cycloadenosine concentration detection of the present invention;

Figure 24 is the chromatogram A of the S4-r001.d group of yeast cycloadenosine concentration detection of the present invention;

FIG. 25 is the chromatogram B of the S4-r001.d group for the detection of the concentration of cyclic adenosine monophosphate in yeast of the present invention;

Description of the labels in the figure:

1. Container body, 2. Limiting table, 3. Separation screen, 301. Floating ring, 302. Net frame, 303. Capture fiber clusters Electric heating plate.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. The embodiments of the present invention, and all other embodiments obtained by those of ordinary skill in the art without creative work, fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the orientations or positional relationships indicated by the terms "upper", "lower", "inner", "outer", "top/bottom", etc. are based on the orientations shown in the accompanying drawings Or the positional relationship is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

In the description of the present invention, it should be noted that, unless otherwise expressly specified and limited, the terms "installation", "provided with", "sleeve/connection", "connection", etc., should be understood in a broad sense, such as " "connection", which can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be a matching type component Internal connectivity. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific situations.

Example 1:

Please refer to Figure 1-2, a method for extracting cyclic adenosine monophosphate, which mainly includes the following steps:

S1, jujube screening, the picked jujubes are screened, select 8 minutes of ripe, cyan jujubes with full particles, wherein the jujubes are preferably Yiwu jujubes;

S2. Bubble with hot water, quickly scald the selected jujubes in boiling water, the scalding time is 2 seconds, place the scalded jujubes in an airtight thermal container and simmer for 2 hours, and then simmer the simmered jujubes for 2 hours. Take out the jujube and lay it flat on the straw mat, and expose it to the sun until the jujube skin turns red, and the jujube skin is twisted into a wrinkled skin. After cleaning the jujube, it is boiled in boiling water, Put it in a container filled with boiling water and cook, add cold water after the boiling water boils and turn it gently, repeat 3 times, the cooking time is 15min, until the body is soft and can touch the jujube core, and then put the cooked jujube Exposure to the sun for 4 hours;

S3, drying, laying the jujube soaked in hot water on the Kang Kang, covering the surface of the jujube with a double-silk sack, and baking for 5 consecutive days, turning it every 2 hours;

S4, the preparation of jujube paste, select 50kg of jujube and smash it to form a powder, then add 50kg of glutinous rice and 100kg of clean water and stir evenly to form a yeast body, steam it in a steamer, take it out and cool it, add yeast and stir and then close the fermentation for 30 days ;

S5, extraction of cyclic adenosine monophosphate, after the yeast body is fermented and matured, the liquid cyclic adenosine monophosphate and the solid cyclic adenosine monophosphate are extracted by catalytic reaction of biological enzymes.

Please refer to Figure 8-25, which is the number table, chromatogram and analysis chart of the concentration measurement of yeast cyclic adenosine monophosphate in this protocol. The normal sample components are A-r001.d, B-r001.d and C- There are three groups of r001.d, and four calibration groups are set up using the same test method at the same time: S1-r001d, S2-r001d, S3-r001d and S4-r001d, where the calibration group is used to check the accuracy of the detection method, which is the pre-configured concentration The liquid is roughly an arithmetic progression, and a coordinate system is established for the concentration and response data of the samples in the calibration group, and a straight line can be fitted. There is also an obvious linear relationship with the response value, that is, the cyclic adenosine monophosphate concentration data obtained in this detection method is true and reliable.

It can be known from the chromatogram of the final product that the concentration of cyclic adenosine in the final yeast produced by this scheme is 250-300 mg/L.

Finally, the technology of extracting cyclic adenosine monophosphate with biological enzymes refers to the use of biodegrading enzymes to decompose jujube cell tissues, release cyclic adenosine monophosphate, and then carry out the extraction work. disclosed in detail.

In step S2, in the hot water soaking, when the jujube is cleaned, vegetable oil is added to the cleaning water to increase the cleaning effect.

Please refer to Fig. 3-7, in step S2, in the hot water bubbling, during the boiling process, the container containing the boiling water includes a container body 1, the lower end of the container body 1 is fixedly connected with a water inlet pipe 4, and the water inlet pipe 4 is inserted with a The sealing tube 6 that matches itself, a plurality of connecting through holes 7 are drilled on the side wall of the sealing tube 6, and the plurality of connecting through holes 7 communicate with the inside and the outside world, and the outer side of the connecting through hole 7 is sleeved with a tension spring 8 , the two ends of the tension spring 8 are respectively fixedly connected with the water inlet pipe 4 and the connecting through hole 7, the upper end of the water inlet pipe 4 is fixedly connected with a magnetic iron ring 5, and the upper part of the sealing pipe 6 is made of waterproof electromagnets. The bottom plate of 1 is fixedly connected with a fan-shaped electric heating plate 9, wherein the water inlet pipe 4 is communicated with the water delivery pipe, and the water delivery pipe can transport distilled water with a temperature of 10 degrees Celsius to the container main body 1 through the water inlet pipe 4 at a constant pressure. The adsorption between the iron ring 5 and the sealing tube 6 and the balance between the water pressure in the container main body 1 and the tension force generated by the deformation of the tension spring 8 and the water supply water pressure realize the automatic addition of water into the container main body 1, and boil it in boiling water. When the system is in normal operation, the liquid level in the container body 1 is at a high level, and the hydraulic pressure on the sealing tube 6 is relatively large. , the sealing pipe 6 is located in the water inlet pipe 4 and will not carry out water delivery work. When the liquid level in the container body 1 is at a low level, the hydraulic pressure received by the sealing pipe 6 is reduced. At this time, the tensile force and Under the combined action of water delivery and water pressure, the sealing tube 6 is lifted up, and low-temperature distilled water is delivered into the container main body 1 through the connecting through hole 7, and the water filling work is automatically completed, and the cold water will gradually heat up during the rising process, which greatly reduces the boiling temperature. The temperature difference that the jujube is subjected to reduces the damage to the jujube and increases the cooking effect.

In particular, in the above description process, the gravity of the sealing tube 6 is not considered in the force analysis, because the gravity of the sealing tube 6 is fixed, which can be regarded as a fixed parameter of the system, so it is not quantitatively analyzed. Since the size of the adsorption force between the magnetic iron ring 5 and the sealing tube 6 is related to the distance between the two positions, after the liquid level in the container body 1 reaches the set position, the magnetic iron ring 5 and the sealing tube 6 are adsorbed, The magnetic iron ring 5 and the sealing tube will not be separated again due to the slight change of the liquid level in the container body 1, so that the water injection work is not easy to be frequent. The loss of distilled water in the container body 1 is mainly caused by the vaporization of boiling water.

The cross section of the inlet wall of the lower end of the sealing tube 6 is an inclined surface, and the top of the inner wall of the sealing tube 6 is an arc surface, which reduces the wear caused by water scouring and increases the service life of the sealing tube 6. The water outlet of the water inlet tube 4 is higher than the container body 1. The bottom plate makes it difficult for the jujube sundries generated during the cooking process to settle on the bottom plate of the container body 1 to be fixed on the upper side of the sealing pipe 6, which will affect the normal operation of the water inlet pipe 4 and the sealing pipe 6.

The container main body 1 is provided with a self-matching partition screen 3, which is suspended in the boiling water in the container main body 1 to screen out the large particles of debris that fall off during the cooking process of jujube, and it is not easy for the lower side. The normal operation of the water inlet pipe 4 and the sealing pipe 6 is affected. The container main body 1 is fixedly connected to the limit table 2. The limit table 2 is located on the upper side of the separation screen 3. The limit table 2 has a limiting effect on the separation screen 3, so that the separation The screen 3 floats to the surface of the boiling water in the container body 1, which is not easy to affect the boiled jujube. The separation screen 3 includes a floating ring 301, and the floating ring 301 is fixedly connected with a mesh frame 302 that matches itself, wherein the floating ring 301 The mesh frame 302 and the mesh frame 302 are all made of elastic material, which is convenient to take out the floating ring 301 and the mesh frame 302 from the container body 1. The floating ring 301 has a tendency to expand outward. The stability of the mesh frame 301 and the mesh frame 302 is not easy to fail due to excessive deformation in the waste water. The lower end of the mesh frame 302 is fixedly connected with a capturing fiber cluster 303. The capturing fiber cluster 303 includes a plurality of elastic fibers, and the plurality of elastic fibers are three-dimensional. Spiral shape, adjacent elastic fibers are entangled together to form a three-dimensional spatial structure, which can capture the sundries that fall off during the cooking process of jujube, which is convenient for subsequent unified cleaning.

In this scheme, jujube is selected, and through the preparation process of screening, hot water soaking, drying, jujube paste preparation and adenosine cyclophosphate extraction, adenosine cyclophosphate can be extracted from jujube rich in cyclophosphate. The use of chemical agents is reduced in the process, and the final product color of cyclophosphate is not easily affected.

The obtained cyclophosphate cyclophosphate products are often used in oral liquids, paste recipes and soups.

In the process of jujube processing, a special container is used to cook jujube, and low-temperature distilled water is sent into the container body 1 through the connecting through hole 7, and the water filling work is automatically completed. The temperature difference caused by cooking jujube reduces the damage caused to the jujube, increases the cooking effect, reduces the influence of the measurement difference caused by artificial water addition on the jujube cooking, and increases the subsequent extraction effect. The internal separation screen 3 can capture the sundries that fall off during the cooking process of the jujube, which is convenient for subsequent unified cleaning.

The above description is only a preferred embodiment of the present invention; however, the protection scope of the present invention is not limited thereto. Any person skilled in the art who is familiar with the technical scope of the present invention, according to the technical solution of the present invention and its improvement concept, equivalently replaces or changes, should be covered within the protection scope of the present invention.

Claims (10)

1. an extraction method of cyclic adenosine monophosphate, is characterized in that: mainly comprise the following steps: S1, jujube screening, the picked jujubes are screened, select 8 minutes of ripe, cyan jujubes with full particles, wherein the jujubes are preferably Yiwu jujubes; S2. Bubble with hot water, quickly scald the selected jujubes in boiling water, the scalding time is 2 seconds, place the scalded jujubes in an airtight thermal container and simmer for 2 hours, and then simmer the simmered jujubes for 2 hours. Take out the jujube and lay it flat on the straw mat, and expose it to the sun until the jujube skin turns red, and the jujube skin is twisted into a wrinkled skin. After cleaning the jujube, it is boiled in boiling water, Put it in a container filled with boiling water and cook, add cold water after the boiling water boils and turn it gently, repeat 3 times, the cooking time is 15min, until the body is soft and can touch the jujube core, and then put the cooked jujube Exposure to the sun for 4 hours; S3, drying, laying the jujube soaked in hot water on the Kang Kang, covering the surface of the jujube with a double-silk sack, and baking for 5 consecutive days, turning it every 2 hours; S4, the preparation of jujube paste, select 50kg of jujube and smash it to form a powder, then add 50kg of glutinous rice and 100kg of clean water and stir evenly to form a yeast body, steam it in a steamer, take it out and cool it, add yeast and stir and then close the fermentation for 30 days ; S5, extraction of cyclic adenosine monophosphate, after the yeast body is fermented and matured, the liquid cyclic adenosine monophosphate and the solid cyclic adenosine monophosphate are extracted by catalytic reaction of biological enzymes. 2 . The method for extracting adenosine cyclophosphate according to claim 1 , wherein in the step S2 and hot water infiltration, when jujube is washed, vegetable oil is added to the washing water. 3 . 3. The method for extracting adenosine cyclophosphate according to claim 1, characterized in that: in the step S2, hot water infiltration, and in the boiling water cooking process, the container containing the boiling water comprises a container body (1), the lower end of the container body (1) is fixedly connected with a water inlet pipe (4), the water inlet pipe (4) is inserted with a sealing pipe (6) matching itself, and the sealing pipe (6) A plurality of connecting through holes (7) are drilled on the side wall of the connecting hole (7), and the plurality of connecting through holes (7) communicate with the inside and the outside world, and a tension spring (8) is sleeved on the outer side of the connecting through hole (7). The two ends of the tension spring (8) are respectively fixedly connected with the water inlet pipe (4) and the connecting through hole (7), and the upper end of the water inlet pipe (4) is fixedly connected with a magnetic iron ring (5). The upper end part of the sealing tube (6) is made of waterproof electromagnets, and a fan-shaped electric heating plate (9) is fixedly connected to the bottom plate of the container body (1). 4. The method for extracting adenosine cyclophosphate according to claim 3, wherein the cross section of the inlet wall of the lower end of the sealing tube (6) is an inclined plane, and the top of the inner wall of the sealing tube (6) is an arc noodle. 5 . The method for extracting adenosine cyclophosphate according to claim 3 , wherein the water outlet of the water inlet pipe ( 4 ) is higher than the bottom plate of the container body ( 1 ). 6 . 6. The method for extracting adenosine cyclophosphate according to claim 3, wherein the container body (1) is provided with a self-matching dividing sieve (3), and the dividing sieve (3) Suspended in boiling water in the container body (1). 7 . The method for extracting adenosine cyclophosphate according to claim 6 , wherein the container body ( 1 ) is fixedly connected to a limiting stage ( 2 ), and the limiting stage ( 2 ) is located on a separating sieve. 8 . (3) on the upper side. 8 . The method for extracting cyclic adenosine monophosphate according to claim 6 , wherein the separating sieve ( 3 ) comprises a floating ring ( 301 ), and the floating ring ( 301 ) is fixedly connected with itself matching screen frame (302). 9 . The method for extracting cyclic adenosine according to claim 7 , wherein the floating ring (301) has a tendency to expand outward, and the mesh frame (302) is woven from an elastic material. 10 . . 10. The method for extracting adenosine cyclophosphate according to claim 7, wherein the lower end of the mesh frame (302) is fixedly connected with a capturing fiber cluster (303), and the capturing fiber cluster (303) It includes a plurality of elastic fibers, and the plurality of elastic fibers are in a three-dimensional spiral shape, and the adjacent elastic fibers are entangled together to form a three-dimensional spatial structure.

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