CN111658598A - Composition for improving scalp ecology and preparation method thereof - Google Patents
Composition for improving scalp ecology and preparation method thereof Download PDFInfo
- Publication number
- CN111658598A CN111658598A CN202010470572.1A CN202010470572A CN111658598A CN 111658598 A CN111658598 A CN 111658598A CN 202010470572 A CN202010470572 A CN 202010470572A CN 111658598 A CN111658598 A CN 111658598A
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- Prior art keywords
- fermentation
- scalp
- filtrate
- bacillus coagulans
- fructo
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/006—Antidandruff preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
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- Epidemiology (AREA)
- Communicable Diseases (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a composition for improving scalp ecology and a preparation method thereof, relating to the technical field of scalp care and comprising the following components: bacillus coagulans fermented filtrate, Bifidobacterium breve fermented filtrate, rhizoma Zingiberis recens extract and fructo-oligosaccharide; the composition for improving the scalp ecology can effectively inhibit the growth of harmful bacteria and has good promotion effect on hair growth; the composition emulsion provided by the invention is added, so that the composition emulsion has a good barrier repair effect on human skin.
Description
Technical Field
The invention relates to the technical field of scalp care, and particularly relates to a composition for improving scalp ecology and a preparation method thereof.
Background
Compared with other parts of skin, the scalp has developed sebaceous sweat glands, abundant sebum secretion, and high epidermis keratinization and renewal speed of the scalp, which is about twice that of other skin, and most cuticles are thick and compact. With the accelerated pace of life, increased working pressure, irregular life, environmental pollution and other problems, more and more people face the problems of alopecia, white hair, hair oil and the like. It is reported that 99% of people in China are troubled by various scalp problems, wherein 35% of people have problems of losing hair, losing scalp hair oil, etc., about 34% of people have problems of scurf and itching, and about 20% of people have problems of dry hair, damage and easy breakage. In 2002, the health standards defined by the world health organization describe scalp health as "hair is elastic, natural shiny, without dandruff". Scalp problems have become one of the concerns of people today.
The healthy scalp ecosystem is maintained by three major balances: lipid, flora and metabolic balance. When the secretion of oil and fat of scalp is unbalanced, the oil of scalp is greasy; when the scalp flora is unbalanced in environment and a large amount of harmful bacteria are bred, the phenomenon of itching can occur; the stratum corneum of the scalp is metabolized too quickly and falls off to form dandruff. Scalp problems caused by imbalance of ecological environment of scalp include oily hair, itching hair, dandruff, dry hair, alopecia, etc.
From a microscopic view, healthy scalp microecology formed by scalp microorganisms can play a role in skin self-cleaning and immunity, can participate in cell metabolism and assist in the exertion of skin physiological functions; from a macroscopic perspective, the physiological environment of the scalp, such as sebum content, moisture content, and ph, all reflect the health of the scalp and directly affect hair growth.
At present, the hair washing product relates to a scalp care concept less, and although the added raw materials can remove dirt and dandruff, the added raw materials have certain damage to the scalp. The chemical bacteriostatic agent mainly comprises Zinc Pyrithione (ZPT), piroctone olamine salt (OCT) and Climbazole (CLM), has the bactericidal effect on normal bacteria, is easy to damage the balance of flora, and can also hinder the scalp from absorbing beneficial functional components due to improper use, thereby causing abnormal metabolism of hair environment. The plant bacteriostatic agent mainly comprises extracts of wrinkled gianthyssop herb, garden balsam stem, rhubarb, amur corktree bark, purslane, common cnidium fruit, ginger and camellia seed, and the like, has relatively small injury to scalp, but has weak antibacterial property.
Therefore, it is important to develop a composition capable of balancing scalp ecology by analyzing scalp problems in directions of unbalance of scalp flora, damage of barrier function and apoptosis of hair follicle cells, so as to fundamentally solve the scalp problems and achieve the effects of caring scalp, relieving scalp inflammation and preventing alopecia.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a composition for improving scalp ecology, a preparation method and application thereof in scalp care, and the composition can effectively balance scalp flora, repair scalp barrier structure and regulate hair follicle growth cycle.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
the invention provides a composition for improving scalp ecology, which comprises the following components: bacillus coagulans fermented filtrate, Bifidobacterium breve fermented filtrate, rhizoma Zingiberis recens extract and fructo-oligosaccharide.
The further scheme of the scheme is that the weight percentage of each component is as follows:
40.0-50.0% of bacillus coagulans fermentation filtrate
Bifidobacterium breve fermentation filtrate 20.0-30.0%
0.5 to 5 percent of fructo-oligosaccharide
Ginger extract 0.1-2%
The balance being water.
The further scheme of the scheme is that the weight percentage of each component is as follows:
bacillus coagulans fermentation filtrate 40%
Bifidobacterium breve fermentation filtrate 30.0%
2 percent of fructo-oligosaccharide
1 percent of ginger extract
The balance being water.
In a further scheme of the scheme, the bacillus coagulans fermentation liquor is formed by fermenting bacillus coagulans BC99, and the lactobacillus brevis fermentation filtrate is formed by fermenting lactobacillus brevis BBr 60.
In another aspect, the present invention also provides a method for preparing the composition, comprising the steps of:
s1, dissolving glycerol tubes of bacillus coagulans BC99 and lactobacillus brevis BBr60, inoculating the dissolved glycerol tubes into PCA and MRS test tube culture media respectively to obtain first-stage seed liquid, respectively inoculating the first-stage seed liquid into corresponding seed culture media to obtain second-stage seed liquid, respectively inoculating the second-stage seed liquid into fermentation culture media to perform high-density fermentation, and respectively collecting the fermentation liquids;
s2, respectively centrifuging the fermentation liquor, collecting supernatant, and filtering with a filter membrane to obtain fermentation filtrate;
s3, fructo-oligosaccharide and ginger extract, drying until the water content is lower than 1%, and using as auxiliary material;
s4, compounding the fermentation filtrate and the auxiliary materials according to a preset proportion, and uniformly mixing to obtain the composition.
Specifically, the centrifugal treatment temperature in S2 is 3-5 ℃, and 4 ℃ is preferred; the centrifugal processing rotating speed is 6000-; the centrifugation time is 12-20min, preferably 15 min.
Specifically, in the S3, the drying condition of the fructo-oligosaccharide is drying for 2-3h at 115 ℃; the rhizoma Zingiberis recens extract is oven-dried at 85 deg.C for 6-7 h.
Specifically, in S4, the mass ratio of the fermentation filtrate to the auxiliary materials is (20-60):1, and the mass ratio of the bacillus coagulans fermentation filtrate to the bifidobacterium breve fermentation filtrate is (1-2.5): 1.
in another aspect, the present invention also provides a use of the composition in scalp care products including shampoos, conditioners, hair rinses and hair sprays.
Compared with the prior art, the technical scheme of the invention has the following advantages and beneficial effects: the invention provides a composition for improving scalp ecology, which comprises the following components: bacillus coagulans fermented filtrate, Bifidobacterium breve fermented filtrate, rhizoma Zingiberis recens extract and fructo-oligosaccharide; the composition for improving the scalp ecology can effectively inhibit the growth of harmful bacteria and has good promotion effect on hair growth; the composition emulsion provided by the invention is added, so that the composition emulsion has a good barrier repair effect on human skin.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without any inventive step, shall fall within the scope of the present invention.
It is noted that the terms "comprises" and "comprising," and any variations thereof, in the description and claims of the present invention, are intended to cover a non-exclusive inclusion, such that a process, method, apparatus, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
The Bacillus coagulans (Bacillus coagulans) BC99 used in the following examples has a preservation number of CGMCC No.19487, a preservation date of 3 and 18 days in 2020, a preservation unit of China general microbiological culture Collection center, and a preservation address of No. 3 of Xilu 1 Beijing north township area in the sunward area.
The methods and materials mentioned in the examples below are, unless otherwise specified, those conventional in the art.
Example 1
(1) Preparation of bacillus coagulans BC99 fermentation filtrate:
dissolving a glycerol tube of bacillus coagulans BC99, inoculating the dissolved glycerol tube into a PCA liquid culture medium, culturing to obtain a first-stage seed solution, inoculating 2mL of the first-stage seed solution into 100mL of the PCA liquid culture medium to obtain a second-stage seed solution, and transferring the second-stage seed solution into a fermentation culture medium according to the inoculation amount of 2% (volume ratio) for high-density fermentation. After the fermentation is finished, the zymocyte liquid is centrifuged (the centrifugation temperature is 3-5 ℃, the centrifugation rotation speed is 6000-.
(2) Preparation of Bifidobacterium breve BBr60 fermentation filtrate
Dissolving a glycerol tube of Bifidobacterium breve BBr60, inoculating the dissolved glycerol tube into an MRS liquid culture medium, performing anaerobic static culture at 37 ℃ to obtain a primary seed solution, inoculating 2mL of the primary seed solution bacterial liquid into 100mL of the liquid culture medium to obtain a secondary seed solution, and transferring the secondary seed solution into a fermentation culture medium according to the inoculation amount of 2% (volume ratio) for high-density fermentation. And (3) obtaining a zymophyte liquid after the fermentation is finished, centrifuging the zymophyte liquid (the centrifugation temperature is 3-5 ℃, the centrifugation rotation speed is 6000-.
(3) Preparation of fructooligosaccharides
Drying the purchased fructo-oligosaccharide at 115 ℃ for 2-3h to obtain the fructo-oligosaccharide with the water content lower than 1% for later use;
(4) preparation of ginger extract
Oven drying the obtained rhizoma Zingiberis recens extract at 85 deg.C for 6-7 hr to obtain rhizoma Zingiberis recens extract with water content of less than 1%.
Example 2
The bacillus coagulans BC99 fermented filtrate, the bifidobacterium breve BBr60 fermented filtrate, the fructo-oligosaccharide, the ginger extract and water in the example 1 are compounded according to the proportion shown in the table 1 and are uniformly mixed to obtain the probiotic composition for improving the scalp ecology.
Table 1 ratio table of probiotic compositions for improving scalp ecology
| Components | Mass ratio (%) |
| Bacillus coagulans fermentation filtrate | 40 |
| Bifidobacterium breve fermentation filtrate | 30 |
| Ginger extract | 1 |
| Fructo-oligosaccharide | 2 |
| Water (W) | 25 |
Example 3
The bacillus coagulans BC99 fermented filtrate, the bifidobacterium breve BBr60 fermented filtrate, the fructo-oligosaccharide, the ginger extract and water in example 1 are compounded according to the proportion shown in the table 2 and are uniformly mixed to obtain the probiotic composition for improving the scalp ecology.
Table 2 ratio table of probiotic compositions for improving scalp ecology
Example 4
The bacillus coagulans BC99 fermented filtrate, the bifidobacterium breve BBr60 fermented filtrate, the fructo-oligosaccharide, the ginger extract and the water in the example 1 are compounded according to the proportion shown in the table 3 and are uniformly mixed to obtain the probiotic composition for improving the scalp ecology.
Table 3 ratio table of probiotic compositions for improving scalp ecology
| Components | Mass ratio (%) |
| Bacillus coagulans fermentation filtrate | 50 |
| Bifidobacterium breve fermentation filtrate | 25 |
| Ginger extract | 0.4 |
| Fructo-oligosaccharide | 1 |
| Water (W) | 23.6 |
Example 5
1) Toxicology test
The test method comprises the following steps: the probiotic compositions prepared in examples 2-4 were evaluated for toxicity according to the cosmetic safety specifications (2015 edition) acute transdermal toxicity test, and transdermal LD50 was combined with the observed toxicity and autopsy findings for evaluation.
The results of the acute percutaneous toxicity test are as follows: the skin toxicity is classified as actual non-toxicity, no death of guinea pigs occurs in the test process, the health condition of the guinea pigs is good, and the behavior is normal.
2) Skin allergy test
The test method comprises the following steps: skin allergy test was performed on probiotic compositions prepared In examples 2-4 according to the Skin occlusion patch test of cosmetic safety technical Specification (2015 edition) and the Skin sensitization test (DPRA) of OECD TG442C In chemical Skin sensitivity (DPRA), and whether the test substance was an allergen (i.e., a sensitizer) was determined according to whether the test substance was positive.
The skin allergy test results are: non-sensitizing property.
3) Skin irritation/Corrosion test
The test method comprises the following steps: the probiotic compositions prepared In examples 2-4 were applied to the surface of skin models according to the skin irritation/corrosivity Test OECD TG 439 (In vitro skin irritation: Reconstructed Human Epidermides (RHE) Test Method (2015) for differentiation between skin irritants and non-irritants, using relative cell activity as a criterion.
The skin irritation/corrosivity test results were: has no irritation.
4) Eye irritation/Corrosion test
The test method comprises the following steps: the probiotic compositions prepared in examples 2-4 were scored for eye irritation according to SN/T2329-containing 2009 Test for cosmetic eye irritation/corrosiveness of chick embryo chorioallantoic membrane and OECD TG 437 Test for bone coral Opacity and Permeability Test Method (2017) eye irritation/corrosiveness Test.
According to the criteria, the results of the eye irritation/corrosivity test are: it is non/light irritative.
Example 6
Malassezia antagonism experiment
1) Selecting strains
Malassezia furfur (m.fursulfur) CBS1878, malassezia furfur (m.sympodialis) CBS7222, malassezia globosa (m.globosa) CBS7966, malassezia spongiosa (m.sloofiae) CBS7956, malassezia blumea obtusifolia (m.obtusa) CBS7876
2) Culturing of bacterial strains
LAN medium: weighing 10g of peptone, 5g of glucose, 1g of yeast extract, 4g of dried bovine bile salt, 1g of glycerol, 0.5g of glyceryl monostearate, 600.5 mL of tween-600, 10g of whole milk, 12g of agar and 0.5g of chloramphenicol, and adding 1L of pure water for dissolving to obtain a mixed solution; boiling the mixture for 1min, adding 20g oleum Olivarum, autoclaving at 121 deg.C for 15min, shaking, and cooling to about 50 deg.C; then 0.5g of cycloheximide was added to the mixture under aseptic conditions, shaken well and poured onto a plate for further use.
Malassezia liquid medium (g/L): 20g of maltose, 2g of yeast extract, 20g of glucose and 20g of olive oil, and after the materials are dissolved by adding distilled water, the materials are sterilized at 121 ℃ under high pressure.
3) Liquid medicine sample adding
Preparation of comparative example 1: 5g of the fructo-oligosaccharide prepared in example 1 and 2g of ginger extract are weighed, dissolved in 93g of water and mixed uniformly for later use.
Preparation of comparative example 2: weighing 30g of bifidobacterium breve BBr6 fermentation liquor, 5g of fructo-oligosaccharide and 2g of ginger extract, dissolving in 63g of water, and uniformly mixing for later use.
Diluting: the probiotic compositions prepared in examples 2-4 and the compositions prepared in comparative examples 1-2 were diluted to 10 gradients with a maximum concentration of 0.5g/mL by a two-fold dilution method using liquid medium, respectively.
Adding sample of liquid medicine: in a 96-well culture plate, 100mL of 10 gradient liquid medicines are added into the 1 st to 10 th wells, the concentration of the liquid medicines from the first well to the 10 th well is changed from high to low, 100mL and 200mL of liquid culture medium are respectively added into the 11 th well and the 12 th well, which are respectively used as growth control and blank control, 3 rows are simultaneously carried out on each sample, and the average value of 3 numbers is obtained.
4) Preparation and inoculation of bacterial liquid
Preparation of bacterial liquid, continuously subculturing Malassezia (culturing at 32 deg.C for 4 days) for 2 times, eluting purified colony with sterile normal saline to obtain bacterial suspension, measuring OD660 to be 1, and counting on blood cell counting plate to be (1-5) × 106CFU/mL, then diluting the bacterial liquid with a liquid culture medium to adjust the bacterial content to (1-5) × 104CFU/mL。
The inoculation process comprises adding 100mL of bacteria liquid into 1-11 rows of 96-well plate to obtain final bacteria concentration of (0.5-2.5) × 104CFU/mL。
Culturing and judging results: and placing the inoculated culture plate in an incubator for incubation at 35 ℃, observing the result after culturing for 72h, and observing the growth condition of the bacterial colony by naked eyes, wherein the growth condition of the positive control hole is good, the growth of the bacterial colony is not inhibited, and the growth of the bacterial colony is inhibited.
The experimental results are shown in table 4, and examples 2-4 all have good bacteriostatic effects on different malassezia bacteria, and compared with comparative examples 1-2, the probiotic composition added with the bacillus coagulans fermented filtrate and the bifidobacterium breve fermented filtrate has stronger bacteriostatic ability.
TABLE 4 MIC (g/mL) of probiotic compositions against Malassezia
MIC value: the lowest drug concentration without colony growth.
Example 7
Influence on the transepidermal Water loss Rate (TEWL value) of the skin
Subject requirements and test conditions: healthy subjects without skin disease history and cosmetic allergy history in the age of 20-55 are selected according to the voluntary principle. Before the test, the test part (face and forearm) needs to be cleaned, and the test subject can not drink water, eat food and do strenuous activities within 30min before the test, and other similar products can not be used. The test conditions are that the temperature is 20-22 ℃ and the relative humidity is 50%.
Basic emulsion: weighing 8 parts of emulsifier, 5 parts of glycerol, 0.05 part of thickener, 0.1 part of preservative and 86.85 parts of water, and uniformly stirring to obtain a basic emulsion;
test samples: 10% (mass ratio) of the probiotic composition of examples 2 to 4 and comparative examples 1 to 2 prepared in example 6 were added to the base emulsion, and stirred at a high speed uniformly to serve as test samples; to the base emulsion, 10% water was added and stirred at high speed until homogeneous as a control.
Skin test: 8 testees are randomly selected for each test sample, the positions of the facial test points are marked, about 1g of sample is uniformly smeared on the face after each tester cleans the face in the morning and at night every day, and different samples cannot be used alternately. The transepidermal water loss (TEWL) of the skin was measured with a transepidermal water loss test probe for 5d, 10d, and 20d consecutive before use of the sample, and 20s was taken for each test point and averaged.
TABLE 5 Effect of test samples on the transepidermal Water loss rate (TEWL value) of the skin
Note: comparison with blank control group: p < 0.01, x: p is less than 0.05
The test results are shown in Table 5, and it can be seen from the change in TEWL value within 20 days of continuous use of the samples that all samples have a certain reduction effect on the skin TEWL, wherein the test samples treated in examples 2-4 have significant differences (p < 0.01) compared with the control group; comparative example 1 the treated test sample was not significantly different from the control; comparative example 2 the treated test sample was significantly different (p < 0.05) from the control group, indicating that bacillus coagulans BC99 fermentation filtrate and bifidobacterium breve BBr66 fermentation filtrate are the main components for promoting skin barrier repair.
Example 8
Influence on Hair growth Rate and Hair growth time
Isolation and organ culture of follicular units: selecting hair follicles in the anagen phase, placing the hair follicles in a 24-well plate with 1 root per well, adding William E serum-free medium and 5% CO2Culturing at 37 deg.C for 12h, and selecting 102 roots of intact hair follicle (i.e. hair follicle with growth rate of about 0.35mm or more in 1 d) with similar physiological growth rate.
Experimental treatment: the experimental groups were: the probiotic compositions prepared in examples 2-4 were formulated into 3 dose groups: 100mg/mL, 50mg/mL, 10 mg/mL; the positive control group was: commercial-octopus 101B hair loss prevention hair tonic; the control group was: and (4) distilled water.
And (3) detection: the hair follicle morphology was observed daily under an inverted microscope and the hair growth length was measured for 12 days, with the results shown in table 6. The farthest distance between the ends of the free-cultured hair was taken as the hair length. The difference in length between the adjacent 2d hairs was taken as the growth rate (mm/d) of the hairs, and the number of days during which the hairs were no longer lengthened compared to the length during the first 1d was taken as the hair growth time.
TABLE 6 Effect of probiotic compositions on Hair growth Rate and Hair growth time
Note: comparison with blank control a: p < 0.01, b: p is less than 0.05;
comparison with positive control group c: p < 0.01, d: p is less than 0.05
As can be seen from Table 6, the probiotic compositions prepared in examples 2-4 have the effect of accelerating the growth rate of hair in the early, middle and late stages of hair growth and can prolong the growth time of hair; the effect of example 2 is optimal, the low, medium and high doses have significant difference (P < 0.01) compared with the blank control group, and the medium and high doses have no obvious difference compared with the positive control group.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A composition for improving the ecology of the scalp, comprising the following components: bacillus coagulans fermented filtrate, Bifidobacterium breve fermented filtrate, rhizoma Zingiberis recens extract and fructo-oligosaccharide.
2. A scalp ecology improving composition according to claim 1, wherein the mass percentages of the components are as follows, calculated as mass percentages:
40.0-50.0% of bacillus coagulans fermentation filtrate
Bifidobacterium breve fermentation filtrate 20.0-30.0%
0.5 to 5 percent of fructo-oligosaccharide
Ginger extract 0.1-2%
The balance being water.
3. A scalp ecology improving composition according to claim 2, characterized in that the mass percentages of the components are as follows, calculated as mass percentages:
bacillus coagulans fermentation filtrate 40%
Bifidobacterium breve fermentation filtrate 30.0%
2 percent of fructo-oligosaccharide
1 percent of ginger extract
The balance being water.
4. A scalp ecology improving composition according to claim 3, wherein the bacillus coagulans fermentation broth is formed by fermentation of bacillus coagulans BC99 and the lactobacillus brevis fermentation filtrate is formed by fermentation of lactobacillus brevis BBr 60.
5. A process for the preparation of a composition according to any one of claims 1 to 4, comprising the steps of:
s1, dissolving glycerol tubes of bacillus coagulans BC99 and lactobacillus brevis BBr60, inoculating the dissolved glycerol tubes into PCA and MRS test tube culture media respectively to obtain first-stage seed liquid, respectively inoculating the first-stage seed liquid into corresponding seed culture media to obtain second-stage seed liquid, respectively inoculating the second-stage seed liquid into fermentation culture media to perform high-density fermentation, and respectively collecting the fermentation liquids;
s2, respectively centrifuging the fermentation liquor, collecting supernatant, and filtering with a filter membrane to obtain fermentation filtrate;
s3, fructo-oligosaccharide and ginger extract, drying until the water content is lower than 1%, and using as auxiliary material;
s4, compounding the fermentation filtrate and the auxiliary materials according to a preset proportion, and uniformly mixing to obtain the composition.
6. The method according to claim 5, wherein the centrifugation temperature in S2 is 3-5 ℃, preferably 4 ℃; the centrifugal processing rotating speed is 6000-; the centrifugation time is 12-20min, preferably 15 min.
7. The preparation method of claim 5, wherein in S3, the drying condition of fructo-oligosaccharide is 115 ℃ for 2-3 h; the rhizoma Zingiberis recens extract is oven-dried at 85 deg.C for 6-7 h.
8. The method according to claim 5, wherein in S4, the mass ratio of the fermentation filtrate to the auxiliary materials is (20-60):1, and the mass ratio of the Bacillus coagulans fermentation filtrate to the Bifidobacterium breve fermentation filtrate is (1-2.5): 1.
9. use of a composition according to any of claims 1 to 4 in a scalp care product comprising a shampoo, conditioner, hair conditioner essence and hair spray.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115252445A (en) * | 2022-07-14 | 2022-11-01 | 苏州猫尔科技有限公司 | Composite micro-ecological oil control hair care composition and hair care product |
| CN117530907A (en) * | 2024-01-05 | 2024-02-09 | 广州优科生物科技有限公司 | A plant fermentation product and application for oil control, soothing and scalp care |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102131495A (en) * | 2008-07-29 | 2011-07-20 | 欧莱雅 | Cosmetic use of microorganism(s) for treatment of scalp disorders |
| CN103622901A (en) * | 2013-11-21 | 2014-03-12 | 广州立白企业集团有限公司 | Skin cleaner composition capable of regulating microecological balance of skin and preparation method thereof |
| CN108524393A (en) * | 2018-06-11 | 2018-09-14 | 广西科技大学 | A kind of ginger shampoo and preparation method thereof |
| CN110787110A (en) * | 2019-12-09 | 2020-02-14 | 广州栋方生物科技股份有限公司 | Composition with hair care effect and preparation method thereof |
-
2020
- 2020-05-28 CN CN202010470572.1A patent/CN111658598A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102131495A (en) * | 2008-07-29 | 2011-07-20 | 欧莱雅 | Cosmetic use of microorganism(s) for treatment of scalp disorders |
| CN103622901A (en) * | 2013-11-21 | 2014-03-12 | 广州立白企业集团有限公司 | Skin cleaner composition capable of regulating microecological balance of skin and preparation method thereof |
| CN108524393A (en) * | 2018-06-11 | 2018-09-14 | 广西科技大学 | A kind of ginger shampoo and preparation method thereof |
| CN110787110A (en) * | 2019-12-09 | 2020-02-14 | 广州栋方生物科技股份有限公司 | Composition with hair care effect and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115252445A (en) * | 2022-07-14 | 2022-11-01 | 苏州猫尔科技有限公司 | Composite micro-ecological oil control hair care composition and hair care product |
| CN115252445B (en) * | 2022-07-14 | 2024-03-05 | 苏州猫尔科技有限公司 | Composite microecological oil-control hair care composition and hair care product |
| CN117530907A (en) * | 2024-01-05 | 2024-02-09 | 广州优科生物科技有限公司 | A plant fermentation product and application for oil control, soothing and scalp care |
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