CN110367375A - A kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation product and beasts, birds and aquatic products breeding feed - Google Patents
A kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation product and beasts, birds and aquatic products breeding feed Download PDFInfo
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- CN110367375A CN110367375A CN201910501842.8A CN201910501842A CN110367375A CN 110367375 A CN110367375 A CN 110367375A CN 201910501842 A CN201910501842 A CN 201910501842A CN 110367375 A CN110367375 A CN 110367375A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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Abstract
The present invention relates to field of feed.The invention discloses a kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation products, are made by astragalus polyose medicinal extract, honeysuckle, dregs of beans, saccharomycete, bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis, neutral proteinase, acid protease and water by Spawn incubation, inoculation fermentation and drying and other steps；The invention also discloses a kind of beasts, birds and aquatic products breeding feed containing Radix Astragali, honeysuckle and dregs of beans combined fermentation product.The combination of Radix Astragali and honeysuckle can not only reduce animal pest bacterium in the present invention, play antibacterial sterilization, and can improve the immunocompetence of body；In addition animal intestinal tract and immune novel, efficient, safe green feed is adjusted in the molten albumen of high amount of acids and high-content lactic acid in fermented feed；Radix Astragali, honeysuckle and dregs of beans combined fermentation product in the present invention have the characteristics that the molten albumen of high amount of acids, organic acid, can cooperate with the cultivation for promoting livestock and poultry, aquatic livestock from many aspects.
Description
Technical field
The present invention relates to field of feed, more particularly, to a kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation product and
Beasts, birds and aquatic products breeding feed.
Background technique
Radix Astragali is a kind of perennial leguminous plant, is the dry root of Inner Mongolia Astragalis and Astragalus membranacus.Traditional Chinese Medicine thinks yellow
Stilbene has an enhancing immune function, blood pressure lowering, hypoglycemic, liver protection, diuresis, anti-aging, antifatigue, resisting stress, antitumor and relatively wide
General antibacterial action.With polysaccharide contained in Radix Astragali, soaping agents, flavonoids and amino acids etc., effective components are related for this.
Radix Astragali equally plays the role of the animals such as poultry, poultry, rabbit and ox very big, can enhance animal immune organ dysfunction, can enhance body
Immunity has antivirus action effect.The crude extract of Radix Astragali can be used as improve food conversion ratio feed addictive and immune increasing
Strong agent.
Honeysuckle also known as honeysuckle etc. are Caprifoliaceae Lonicera perennial evergreen plants, and it is each that honeysuckle is distributed widely in East Asia
State.Not only there is certain ornamental value, economic value and medical value.Studies have shown that honeysuckle causes a disease to many animals
Microorganism has inhibiting effect, has the biological functions such as anti-inflammatory, antibacterial, antiviral.Since the above-mentioned function of honeysuckle makes it
It is had a wide range of applications in livestock and poultry, aquaculture.
The protolysate of the molten albumen, that is, lower molecular weight of acid in fermented bean dregs, including peptide and free amino acid, detection
On show as the albumen that can be dissolved in acid solution.On the one hand the high fermented bean dregs of the molten protein content of acid then reflect dregs of beans raw material
In the small molecular protein that beneficial metabolic product can such as be digested and assimilated after everfermentation: plant polypeptide and small peptide, digestive ferment and
The content of material such as free amino acid are more, and these substances are highly beneficial to growth of animal, they can not only significantly promote to move
Object growth, moreover it is possible to disease preventing and treating.The amino acid of small peptide and high-content can enhance the immune function of macrophage in immunocyte,
Invading virus can be attacked again, therefore can also improve the immunity of animal.The a large amount of digestive ferment generated in fermentation process has
Protease, amylase, non-starch polysaccharide enzyme, phytase, oligosaccharidase, amino oxidase and hydrogen sulfide catabolic enzyme, above-mentioned digestive ferment
Not only assist in digestion dregs of beans, moreover it is possible to help digest other feedstuffs such as corn, wheat bran, the digestion for improving feed ingredient is inhaled
Yield.What on the other hand the molten protein content of acid was high then reflect dregs of beans raw material after everfermentation to high molecular weight protein in dregs of beans
Palliating degradation degree is high.Dregs of beans is the substance after soybean deoils, and is the important component of growth of animal, mainly serves and puts on flesh.Its thick egg
Bai Hanliang is about 43%-46%.Because containing 22-28% or so the polymer substance i.e. institute for being difficult to be digested and assimilated by animal
Commonly referred to as " antigen protein ".By taking pig as an example, piggy can only absorb about 72% crude protein, and big pig can absorb about 78% thick egg
It is white.Dregs of beans is the excellent diet of growth of animal, but feeding is slightly more, just causes diarrhea.Antigen is exactly the substance for causing diarrhea.
Antigen not only reduces the utilization rate of protein, also causing animal, especially young animal (such as suckling piglet) digestive system
Disease.Using screening, mutagenesis Aerobic-anaerobic bacterium fermentation time difference and be combined fermentation, and depth growth makes antigen
Cell wall, peptide bond can largely be removed antigen by abundant enzymatic hydrolysis rupture.Many experiments show the technique that fermentation enzymatic hydrolysis is used only
The removal rate of fermented bean dregs antigen afterwards and the amount of the molten albumen of acid are positively correlated.
Containing the lactic acid generated in fermentation process in fermented bean dregs, enzymatic hydrolysis-fermentation energy generates a variety of high-content organic acids,
Middle major part is lactic acid,
It can promote beneficial bacterium in chitling road to grow, and prevent enterogastric diseases；Immunity of organisms can be improved；It can activate and disappear
Change enzymatic activity, such as improves the activity of piglet pepsin；It can delay gastric emptying speed, promote to digest and assimilate, improve feed conversion
Rate promotes growth of animals or poultry；It can promote the absorption of the microelements such as calcium, phosphorus and copper, iron, zinc；It is the important component of milk, can improves
Milk.
It is one of the major way of traditional livestock culture industry Field of Animal Epidemic Disease Control using antibiotic, but the long-term of antibiotic makes
With not only will appear Drug resistance status, but also it will lead to animal product there are medicament residue, and then influence human health.
Summary of the invention
To solve the above problems, the present invention provides one kind height as made from Radix Astragali, honeysuckle and dregs of beans combined fermentation to contain
The molten albumen of amount acid, high-content organic acid and Radix Astragali, honeysuckle and dregs of beans group that body immunity is improved with antibacterial sterilization
Close tunning；
The present invention also provides a kind of, and the beasts, birds and aquatic products containing above-mentioned Radix Astragali, honeysuckle and dregs of beans combined fermentation product cultivate feeding
Material.
A kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation product, are made by following steps:
A) saccharomycete Spawn incubation: is vibrated into lower culture 7.5~8.5 using YPDA culture medium with 28~32 DEG C, 140~160rpm
Bacillus subtilis is vibrated lower culture using LB culture medium with 28~32 DEG C, 140~160rpm by hour obtained saccharomycete bacterium solution
22~26 hours obtained bacillus subtilis bacterium solutions, by lactobacillus acidophilus using MARS culture medium with culture 44 at 34~38 DEG C~
Enterococcus faecalis cultivate 44~52 hours systems at 34~38 DEG C using MARS culture medium by 52 hours obtained lactobacillus acidophilus bacterium solutions
Obtain enterococcus faecalis bacterium solution；
B) raw material preparation: prepare astragalus polyose medicinal extract, honeysuckle, dregs of beans, saccharomycete, bacillus subtilis, lactobacillus acidophilus, excrement
Enterococcus, neutral proteinase, acid protease and water；
C) inoculation fermentation: astragalus polyose medicinal extract is dissolved in the water, and adds cultured saccharomycete bacterium solution, bacillus subtilis
Bacterium solution, lactobacillus acidophilus bacterium solution and enterococcus faecalis bacterium solution are uniformly mixed obtained liquid strain, then mix honeysuckle with dregs of beans
Uniformly obtained solid feed, solid feed and liquid strain combined inoculation is uniform, it is small to be put into fermentation 90~100 in air valve bag
When；During the fermented and cultured, when fermentation temperature is 28~32 DEG C, the 24th~48 hour in the 0th~24 hour time range
Between in range fermentation temperature be 34~38 DEG C, fermentation temperature is 39~41 DEG C, the 72nd hour in the 48th~72 hour time range
Time range fermentation temperature is 42~44 DEG C later；
D) dry: the raw material fermented is dry until moisture is in 10~12wt% at 60~70 DEG C.
Preferably, the concentration of saccharomycete is not less than 10 after step a) Spawn incubation7CFU/mL, bacillus subtilis
Concentration is not less than 108The concentration of CFU/mL, lactobacillus acidophilus are not less than 109The concentration of CFU/mL, enterococcus faecalis are not less than
109CFU/mL。
Preferably, preparing raw material, 2.5~4 parts by weight of honeysuckle, astragalus polyose medicinal extract by formula as below in step b)
4.5~6 parts by weight, 990~995 parts by weight of dregs of beans, 23~26 parts by volume of saccharomycete, 23~26 parts by volume of bacillus subtilis,
23~26 parts by volume of lactobacillus acidophilus, 23~26 parts by volume of enterococcus faecalis, 0.9~1.5 parts by weight of neutral proteinase, acidic protein
2.5~3.5 parts by weight of enzyme, 400~450 parts by weight of water.
Preferably, the enzyme activity of neutral proteinase is not less than 50,000 enzyme activity/g, the enzyme activity of the acid protease is
Not less than 50,000 enzyme activity/g.
Preferably, the protein content of dregs of beans is not less than 46wt%.
Preferably, saccharomycete is S. cervisiae.
Preferably, keeping the sealing of air valve bag exhaust sealing in step c) when fermented and cultured.
Preferably, after solid feed and liquid strain combined inoculation are uniform in step c), moisture content control 40~
45wt%.
Preferably, the weight ratio of neutral proteinase and acid protease is 3:5~9.
A kind of beasts, birds and aquatic products breeding feed contains 8~12wt% and is produced by above-mentioned Radix Astragali, honeysuckle and dregs of beans combined fermentation
Object.
Therefore, the invention has the following advantages: in the present invention combination of Radix Astragali and honeysuckle can not only reduce animal
Harmful bacteria plays antibacterial sterilization, and can improve the immunocompetence of body, fundamentally prevents disease；In addition it ferments
The molten albumen of high amount of acids in feed and high-content lactic acid to animal health, it be adjustable animal intestinal tract be immunized it is new
Type, green feed efficiently, safe；Radix Astragali, honeysuckle and dregs of beans combined fermentation product in the present invention are with high amount of acids
The fermented bean dregs of molten albumen, high-content organic acid can be cooperateed with from antibacterial, strengthen immunity with the various aspects such as animal intestinal tract are adjusted
Promote the cultivation of livestock and poultry, aquatic livestock；It is applied to animal-breeding process, has after animal-breeding pollution-free, residual to human body
The features such as staying toxic side effect small.
Detailed description of the invention
Fig. 1 is Radix Astragali, honeysuckle and dregs of beans combined fermentation product antigen degradation rate gel electrophoresis in the embodiment of the present invention 1
Testing result diagram；
Fig. 2 is that Radix Astragali, honeysuckle and dregs of beans combined fermentation product oligosaccharides degradation rate thin layer chromatography detect in the embodiment of the present invention 1
As a result it illustrates.
Specific embodiment
Further description of the technical solution of the present invention With reference to embodiment.
Obviously, the described embodiments are merely a part of the embodiments of the present invention, instead of all the embodiments.Based on this
Embodiment in invention, all other reality obtained by those of ordinary skill in the art without making creative efforts
Example is applied, shall fall within the protection scope of the present invention.
In the present invention, if not refering in particular to, all equipment and raw material is commercially available or the industry is common,
Method in following embodiments is unless otherwise instructed conventional method in that art.
Embodiment 1
A kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation product, are made by following steps:
A) S. cervisiae Spawn incubation: is vibrated into 8 hours obtained yeast of lower culture using YPDA culture medium with 30 DEG C, 150rpm
Bacillus subtilis is vibrated 24 hours obtained bacillus subtilis of lower culture using LB culture medium with 30 DEG C, 150rpm by bacterium bacterium solution
Bacterium bacterium solution, by lactobacillus acidophilus using MARS culture medium to cultivate 48 hours obtained lactobacillus acidophilus bacterium solutions at 36 DEG C, by excrement intestines
Coccus is using MARS culture medium to cultivate 48 hours obtained enterococcus faecalis bacterium solutions at 36 DEG C；After Spawn incubation, the concentration of saccharomycete
It is 1 × 107CFU/mL, the concentration of bacillus subtilis are 1 × 108CFU/mL, the concentration of lactobacillus acidophilus are 1 × 109CFU/
ML, the concentration of enterococcus faecalis are 1 × 109CFU/mL；
B) raw material preparation: prepare raw material, honeysuckle 3g, astragalus polyose medicinal extract 5g, dregs of beans 992g, saccharomycete by formula as below
25mL, bacillus subtilis 25mL, lactobacillus acidophilus 25mL, enterococcus faecalis 25mL, neutral proteinase 0.9g, acid protease
2.7g, water 430g；The enzyme activity of neutral proteinase is 50,000 enzyme activity/g, and the enzyme activity of acid protease is 50,000 enzyme activity/g, dregs of beans
Protein content be 46wt%；
C) inoculation fermentation: astragalus polyose medicinal extract is dissolved in the water, and adds cultured saccharomycete bacterium solution, bacillus subtilis
Bacterium solution, lactobacillus acidophilus bacterium solution and enterococcus faecalis bacterium solution are uniformly mixed obtained liquid strain, then mix honeysuckle with dregs of beans
Uniformly obtained solid feed, by solid feed, uniform and moisture content is controlled in 43wt% with liquid strain combined inoculation, is put
Air valve bag exhaust sealing is kept to be sealed by fermentation 96 hours in air inlet valve bag；During fermented and cultured, the 0th~24 hour time range
Interior fermentation temperature is 30 DEG C, and fermentation temperature is 36 DEG C in the 24th~48 hour time range, in the 48th~72 hour time range
Fermentation temperature is 40 DEG C, and the 72nd~96 hour time range fermentation temperature is 42 DEG C；
D) dry: the raw material fermented is dry until moisture is in 10wt% at 65 DEG C.
A kind of beasts, birds and aquatic products breeding feed, containing 10wt% by above-mentioned Radix Astragali, honeysuckle and dregs of beans combined fermentation product.
Embodiment 2
A kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation product, are made by following steps:
A) S. cervisiae Spawn incubation: is vibrated into 7.5 hours obtained ferment of lower culture using YPDA culture medium with 28 DEG C, 140rpm
Bacillus subtilis is vibrated 22 hours obtained withered grass gemma of lower culture using LB culture medium with 28 DEG C, 140rpm by female bacterium bacterium solution
Bacillus bacterium solution, by lactobacillus acidophilus using MARS culture medium to cultivate 44 hours obtained lactobacillus acidophilus bacterium solutions at 34 DEG C, by excrement
Enterococcus is using MARS culture medium to cultivate 44 hours obtained enterococcus faecalis bacterium solutions at 34 DEG C；After Spawn incubation, saccharomycete it is dense
Degree is 5 × 107CFU/mL, the concentration of bacillus subtilis are 5 × 108CFU/mL, the concentration of lactobacillus acidophilus are 5 × 109CFU/
ML, the concentration of enterococcus faecalis are 5 × 109CFU/mL；
B) raw material preparation: prepare raw material, honeysuckle 2.5g, astragalus polyose medicinal extract 4.5g, dregs of beans 990g, saccharomycete by formula as below
23mL, bacillus subtilis 23mL, lactobacillus acidophilus 23mL, enterococcus faecalis 23mL, neutral proteinase 1.5g, acid protease
2.5g, water 400g；The enzyme activity of neutral proteinase is 100,000 enzyme activity/g, and the enzyme activity of acid protease is 100,000 enzyme activity/g, beans
The protein content of the dregs of rice is 47wt%；
C) inoculation fermentation: astragalus polyose medicinal extract is dissolved in the water, and adds cultured saccharomycete bacterium solution, bacillus subtilis
Bacterium solution, lactobacillus acidophilus bacterium solution and enterococcus faecalis bacterium solution are uniformly mixed obtained liquid strain, then mix honeysuckle with dregs of beans
Uniformly obtained solid feed, by solid feed, uniform and moisture content is controlled in 40wt% with liquid strain combined inoculation, is put
Air valve bag exhaust sealing is kept to be sealed by fermentation 90 hours in air inlet valve bag；During fermented and cultured, the 0th~24 hour time range
Interior fermentation temperature is 28 DEG C, and fermentation temperature is 34 DEG C in the 24th~48 hour time range, in the 48th~72 hour time range
Fermentation temperature is 39 DEG C, and the 72nd~90 hour time range fermentation temperature is 42 DEG C；
D) dry: the raw material fermented is dry until moisture is in 11wt% at 60 DEG C.
A kind of beasts, birds and aquatic products breeding feed, containing 12wt% by above-mentioned Radix Astragali, honeysuckle and dregs of beans combined fermentation product.
Embodiment 3
A kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation product, are made by following steps:
A) S. cervisiae Spawn incubation: is vibrated into 8.5 hours obtained ferment of lower culture using YPDA culture medium with 32 DEG C, 160rpm
Bacillus subtilis is vibrated 26 hours obtained withered grass gemma of lower culture using LB culture medium with 32 DEG C, 160rpm by female bacterium bacterium solution
Bacillus bacterium solution, by lactobacillus acidophilus using MARS culture medium to cultivate 52 hours obtained lactobacillus acidophilus bacterium solutions at 38 DEG C, by excrement
Enterococcus is using MARS culture medium to cultivate 52 hours obtained enterococcus faecalis bacterium solutions at 38 DEG C；After Spawn incubation, saccharomycete it is dense
Degree is 1 × 108CFU/mL, the concentration of bacillus subtilis are 1 × 109CFU/mL, the concentration of lactobacillus acidophilus is 1 ×
1010CFU/mL, the concentration of enterococcus faecalis are 1 × 1010CFU/mL；
B) raw material preparation: prepare raw material, 4 parts by weight of honeysuckle, 6 parts by weight of astragalus polyose medicinal extract, 995 weight of dregs of beans by formula as below
Amount part, 26 parts by volume of saccharomycete, 26 parts by volume of bacillus subtilis, 26 parts by volume of lactobacillus acidophilus, 26 parts by volume of enterococcus faecalis,
1.2 parts by weight of neutral proteinase, 3.5 parts by weight of acid protease, 450 parts by weight of water；The enzyme activity of neutral proteinase is 150,000
Enzyme activity/g, the enzyme activity of acid protease are 150,000 enzyme activity/g, and the protein content of dregs of beans is 48wt%；
C) inoculation fermentation: astragalus polyose medicinal extract is dissolved in the water, and adds cultured saccharomycete bacterium solution, bacillus subtilis
Bacterium solution, lactobacillus acidophilus bacterium solution and enterococcus faecalis bacterium solution are uniformly mixed obtained liquid strain, then mix honeysuckle with dregs of beans
Uniformly obtained solid feed, by solid feed, uniform and moisture content is controlled in 45wt% with liquid strain combined inoculation, is put
Air valve bag exhaust sealing is kept to be sealed by fermentation 100 hours in air inlet valve bag；During fermented and cultured, the 0th~24 hour time model
Enclosing interior fermentation temperature is 32 DEG C, and fermentation temperature is 38 DEG C in the 24th~48 hour time range, the 48th~72 hour time range
Interior fermentation temperature is 41 DEG C, and the 72nd~100 hour time range fermentation temperature is 44 DEG C；
D) dry: the raw material fermented is dry until moisture is in 12wt% at 70 DEG C.
A kind of beasts, birds and aquatic products breeding feed, containing 8wt% by above-mentioned Radix Astragali, honeysuckle and dregs of beans combined fermentation product.
Radix Astragali, honeysuckle and dregs of beans combined fermentation are studied:
Prepare the fermented bean dregs of a kind of molten albumen of the high amount of acids containing Radix Astragali and honeysuckle, high-content organic acid, technical process
In following index be key: astragalus polyose medicinal extract, honeysuckle, dregs of beans account for the ratio of fermentation raw material, the species in combination of strain and
Ratio, moisture after solid-state inoculation, the adding proportion and size of middle acid protease, the setting etc. of temperature and time in fermentation process
Above five indexs.
1) astragalus polyose medicinal extract: honeysuckle: optimal proportion of the ratio of dregs of beans to livestock and poultry cultivation:
Research shows that astragalus polyose has the function of improving immunity of organisms, its most suitable additive amount in adequate diet is
0.5g/kg, additive amount of the fermented product in full price grain is 10%, therefore ratio of the astragalus polyose in fermented product raw material is about
0.5%；Honeysuckle has the function of antibacterial, has inhibiting effect to a variety of livestock and poultry common pathogens[3], it is in adequate diet
Most suitable additive amount is 0.3g/kg: additive amount of the fermented product in full price grain is 10%, therefore honeysuckle is in fermented product raw material
Ratio be about 0.3%.Ratio of the dregs of beans in fermented product raw material is 99.2%.Such combination both can be improved inherently
Immunity can also play inhibiting effect to pathogen, finally can guarantee that fermented bean dregs provide the vegetable protein of high-content high quality.Group
It closes as shown in the table:
| Additive | It is required that the technical indicator reached | Additive amount |
| Honeysuckle | / | 3g |
| Astragalus polyose medicinal extract | / | 5g |
| Dregs of beans | Albumen >=46.0% | 992g |
To sum up: for the present invention in the raw material of fermentation, the ratio that astragalus polyose medicinal extract accounts for the raw material of full fermentation is 0.5%；Honeysuckle
The ratio for accounting for the raw material of full fermentation should be 0.3%: the ratio that dregs of beans accounts for the raw material of full fermentation should be 99.2%.
2) influence of the type of acid-producing bacteria kind to the content of product lactic acid:
Keep lactic acid content in product higher, it is necessary to which the strain effect for selecting one to two plant of energy lactic acid producing is fermenting microbe, reference
It can apply to the lactic acid bacteria of Feed Manufacturing, finally select the generation of lactobacillus acidophilus, enterococcus faecalis, streptococcus lactis as lactic acid bacteria
Bacterium, following table are influence value of the lactic acid bacteria to product lactic acid content of different genera:
Add enterococcus faecalis combination just to can reach higher lactic acid content with lactobacillus acidophilus as seen from the above table, adds streptococcus lactis
It is not significant to the raising of lactic acid, it is contemplated that cost, the present invention add enterococcus faecalis combination as anaerobic bacteria using lactobacillus acidophilus；
Yeast can produce beta glucan during the fermentation, have good facilitation to the cultivation of beasts, birds and aquatic products, real
Existing industrialized production；Bacillus subtilis can produce a large amount of antibacterial substances in fermentation process, in dregs of beans raw material and fermentation process
Miscellaneous bacteria have apparent inhibiting effect, and can produce the metabolites such as a large amount of highly active protein enzyme, to high molecular weight protein
Degradation plays a major role；Lactobacillus acidophilus can largely generate lactic acid substance；Enterococcus faecalis not only can produce a large amount of lactic acid substance,
And be homology microorganism, it is remarkably improved the intestinal health of animal；
To sum up: present invention fermentation mixes bacterium hair using 4 kinds of bacterium (saccharomycete, bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis)
Ferment can not only sufficiently ferment, and completely remove macromolecular anti-nutrient substance, and can produce a variety of fermentating metabolism product (such as plants
Polypeptide and small peptide, antibacterial peptide, other antibacterial materials, immune polysaccharide, somatomedin, B family vitamin, figure improve promotor,
High-content probiotics, a variety of high activity digestive ferments and lactic acid etc.), guarantee fermentating metabolism product diversity.To guarantee that strain is being sent out
During ferment, the balance of aerobic bacteria and anaerobic bacteria technical process, the balance of fermented product protein content and product energy, according to more
Year knowhow, we use the inoculum density of 4 kinds of bacterium for 1:1:1:1.
3) after solid-state inoculation the size of moisture (being collectively referred to as spice moisture below) to product albumen, the molten protein content of acid
It influences:
Experiment shows that spice moisture can significantly affect the albumen of solid fermentation products, the content of the molten albumen of acid and lactic acid etc., following table
For the albumen and the molten protein value of acid of product after fermenting under different spice moisture conditions:
To reach the albumen value optimal with the molten protein value of acid as seen from table, solid-state inoculation spice moisture should be 43%, at this time dry
Under conditions of matter content 90%, the molten albumen of acid can reach highest 20.85%.
To sum up: present invention fermentation should be 43% using solid-state inoculation spice moisture, and molten albumen sour at this time and protein value are highest
It is horizontal.
4) influence of the adding proportion and size of acid protease to product albumen, the molten protein content of acid in:
Since the pH value of fermentation materials in fermentation process is mainly the neutral process with acidity, therefore for highest raising macromolecular
The degradation of albumen selects the kind of the enzyme added during the fermentation for neutral proteinase and acid protease, and following table is spice
When moisture is 42%, influence of the adding proportion and size of acid protease to product albumen, the molten albumen of acid in difference:
To sum up: select to add neutral proteinase during the fermentation: the ratio of acid protease is 2:6, molten albumen sour at this time with
Protein value is highest level.
5) setting of temperature and time is to product albumen in fermentation process, the influence of the molten protein content of acid:
Present invention fermentation uses 4 kinds of bacterium (saccharomycete, bacillus subtilis, lactobacillus acidophilus, enterococcus faecalis) mixed fungus fermentations, above
The combination of four kinds of bacterium can simplify technique, enable aerobic fermentation and the anaerobic fermentation effect of making the most of the advantage, root within the period frequently
According to the variation of oxygen content in fermentation process and the generation of heat, it is aerobic fermentation, and produces certain thermal energy within first day, oxygen consumption
After to the greatest extent and heat raising enters anaerobic fermentation stage；
The suitable temperature of phase sets makes the better growth and breeding of dominant bacteria in different times, so that four kinds of strains all play
Effect according to material heat production in fermentation process, produces the concentration levels of acid, oxygen, fermentation process is set as following time and temperature
Degree:
To sum up: fermentation process is 96h altogether, and wherein set temperature is 30 DEG C in 0-24h time range, is set in 24-48h time range
Determining temperature is 36 DEG C, and set temperature is 40 DEG C in 48-72h time range, and set temperature is 42 DEG C in 72-96h time range.This
When not only can guarantee aerobic bacteria saccharomycete and bacillus subtilis raised growth breeds and generates beneficial metabolic product, anaerobic bacteria
Breeding can be sufficiently grown and generate beneficial metabolic product, neutral proteinase and the acid protease of addition can also divide greatly in raw material
Son is adequately digested.
Embodiment 1 is made Radix Astragali, honeysuckle and dregs of beans combined fermentation product property and detects:
Wherein Fig. 1 also clearly illustrates Radix Astragali made from embodiment 1, honeysuckle and dregs of beans combined fermentation product antigen degradation rate
Testing result, Fig. 2 clearly illustrate Radix Astragali made from embodiment 1, honeysuckle and dregs of beans combined fermentation product oligosaccharides degradation rate
Testing result.
Known to the above index: 90% or more the macromolecular antigen protein of fermented product of the invention is degraded, raffinose with
90% or more stachyose is degraded, and a large amount of molten albumen of acid is generated, for the beneficial plant polypeptide of growth of animal and small peptide, digestive ferment
With free amino acid isoreactivity substance, a large amount of organic acid, essentially lactic acid, the alternative acidification beneficial to growth of animal are generated
Agent uses.
To sum up not only can inherently it be improved using the combination of astragalus polyose and honeysuckle in the product of the method for the present invention
Immunity of organisms can also play inhibiting effect to pathogen.Unique strain combination can not only be effectively reduced with zymotechnique
Macromolecular antigenic substance and the largely active materials beneficial to bodies are generated, makes the molten albumen of acid, lactic acid content, can digest and assimilate
Protein content all improves than common fermentation dregs of beans value significant.Therefore beasts, birds and aquatic products cultivation it is upper can all or part substitute antibiotics,
Fish meal, enzyme preparation, amino acid and acidulant etc., economic and environment-friendly benefit are very significant.
It should be understood that those skilled in the art, can be improved or be become according to the above description
It changes, and all these modifications and variations should all belong to the protection domain of appended claims of the present invention.
Claims (10)
1. a kind of Radix Astragali, honeysuckle and dregs of beans combined fermentation product, it is characterised in that be made by following steps:
A) saccharomycete Spawn incubation: is vibrated into lower culture 7.5~8.5 using YPDA culture medium with 28~32 DEG C, 140~160rpm
Bacillus subtilis is vibrated lower culture using LB culture medium with 28~32 DEG C, 140~160rpm by hour obtained saccharomycete bacterium solution
22~26 hours obtained bacillus subtilis bacterium solutions, by lactobacillus acidophilus using MARS culture medium with culture 44 at 34~38 DEG C~
Enterococcus faecalis cultivate 44~52 hours systems at 34~38 DEG C using MARS culture medium by 52 hours obtained lactobacillus acidophilus bacterium solutions
Obtain enterococcus faecalis bacterium solution；
B) raw material preparation: prepare astragalus polyose medicinal extract, honeysuckle, dregs of beans, saccharomycete, bacillus subtilis, lactobacillus acidophilus, excrement
Enterococcus, neutral proteinase, acid protease and water；
C) inoculation fermentation: astragalus polyose medicinal extract is dissolved in the water, and adds cultured saccharomycete bacterium solution, bacillus subtilis
Bacterium solution, lactobacillus acidophilus bacterium solution and enterococcus faecalis bacterium solution are uniformly mixed obtained liquid strain, then mix honeysuckle with dregs of beans
Uniformly obtained solid feed, solid feed and liquid strain combined inoculation is uniform, it is small to be put into fermentation 90~100 in air valve bag
When；During the fermented and cultured, when fermentation temperature is 28~32 DEG C, the 24th~48 hour in the 0th~24 hour time range
Between in range fermentation temperature be 34~38 DEG C, fermentation temperature is 39~41 DEG C, the 72nd hour in the 48th~72 hour time range
Time range fermentation temperature is 42~44 DEG C later；
D) dry: the raw material fermented is dry until moisture is in 10~12wt% at 60~70 DEG C.
2. Radix Astragali according to claim 1, honeysuckle and dregs of beans combined fermentation product, it is characterised in that:
After the step a) Spawn incubation, the concentration of saccharomycete is not less than 107The concentration of CFU/mL, bacillus subtilis are not less than
108The concentration of CFU/mL, lactobacillus acidophilus are not less than 109The concentration of CFU/mL, enterococcus faecalis are not less than 109 CFU/mL。
3. a kind of Radix Astragali according to claim 1, honeysuckle and dregs of beans combined fermentation product, it is characterised in that:
Prepare raw material, 2.5~4 parts by weight of honeysuckle, 4.5~6 weight of astragalus polyose medicinal extract by formula as below in the step b)
Part, 990~995 parts by weight of dregs of beans, 23~26 parts by volume of saccharomycete, 23~26 parts by volume of bacillus subtilis, lactobacillus acidophilus
23~26 parts by volume, 23~26 parts by volume of enterococcus faecalis, 0.9~1.5 parts by weight of neutral proteinase, acid protease 2.5~3.5
Parts by weight, 400~450 parts by weight of water.
4. a kind of Radix Astragali according to claim 1 or 3, honeysuckle and dregs of beans combined fermentation product, it is characterised in that:
The enzyme activity of the neutral proteinase is not less than 50,000 enzyme activity/g, and the enzyme activity of the acid protease is not less than 50,000
Enzyme activity/g.
5. a kind of Radix Astragali according to claim 1 or 3, honeysuckle and dregs of beans combined fermentation product, it is characterised in that:
The protein content of the dregs of beans is not less than 46wt%.
6. a kind of Radix Astragali according to claim 1 or 2 or 3, honeysuckle and dregs of beans combined fermentation product, it is characterised in that:
The saccharomycete is S. cervisiae.
7. a kind of Radix Astragali according to claim 1, honeysuckle and dregs of beans combined fermentation product, it is characterised in that:
In the step c) when fermented and cultured, the sealing of air valve bag exhaust sealing is kept.
8. a kind of Radix Astragali according to claim 1, honeysuckle and dregs of beans combined fermentation product, it is characterised in that:
After solid feed and liquid strain combined inoculation are uniform in the step c), moisture content is controlled in 40~45wt%.
9. a kind of Radix Astragali according to claim 1 or 3, honeysuckle and dregs of beans combined fermentation product, it is characterised in that:
The weight ratio of the neutral proteinase and acid protease is 3:5~9.
10. a kind of beasts, birds and aquatic products breeding feed, it is characterised in that:
It contains 8~12wt% by any Radix Astragali of above-described embodiment 1~9, honeysuckle and dregs of beans combined fermentation product.
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