CN106721261A - One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof - Google Patents
One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof Download PDFInfo
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Abstract
It is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof the invention discloses one kind, belongs to fodder compound technical field.The feed is prepared by the raw material of following parts by weight：Citrus pulp, oat bran, 1 part of the saccharomycete mixed fermentation product A of Alfalfa, citrus pulp, oat bran, Alfalfa, 13 parts of the lactic acid bacteria mixed fermentation product B of vegetables, citrus pulp, oat bran, 23 parts of the bacillus mixed fermentation product C of Alfalfa.Fermented by the mixing of citrus pulp, oat bran, Alfalfa and vegetables, pig, the intestinal villus development of chicken and Crypt depth can be significantly improved in pig chicken feed as a kind of fermentable fiber feed applications, improve digestive function and intestinal health state.
Description
Technical field
The invention belongs to fodder compound technical field, and in particular to a kind of oat bran and citrus pulp fiber fermentation feed and
Preparation method.
Background technology
Feed fibre is primarily referred to as the dietary nutrient for being typically difficult to be digested, and mostlys come from the cell membrane of plant, wraps
Containing cellulose, cellulose, natural gum, pectin and lignin etc..Fiber is a kind of complicated mixture, can according to water miscible difference
To be divided into water-soluble fibre (SDF) and water-insoluble fiber (IDF)；In recent years, dietary fiber be further divided into can fermented type it is fine
Peacekeeping can not ferment fiber type.Water insoluble dietary fiber is mainly made up of plant cell wall, and such as cellulose, lignin and half are fine
Dimension element, sesame seed meal, wheat bran, rice bran meal etc., largely exist in the feed.Water-soluble dietary fiber is by Hemicellulose Polysaccharide group
Into being such as present in pectin and gel in oat, barley, beans.It is generally hydrophilic, non-crystalline type fiber, with carbon aquation
Compound is relevant with the metabolism of lipid, is easily soaked by enteron aisle liquid, therefore is easy to be utilized by the Institute of Micro-biology in enteron aisle.Can fermented type
Fiber refers to the part fiber that varying level degree can be resolved into by the microorganism in animal and bird intestines, mainly including levulan,
Pectin, polydextrose and resistant starch etc..Can fermented type dietary fiber livestock and poultry can be promoted to be metabolized, such as its can as prebiotics,
Promote the growth of beneficial bacteria of intestinal tract；SCFA (SCFA) is produced, promotes the absorption of water and sodium, promote mucosal cell proliferation；
Energy is provided；Reduce the pH in lumen.Can not fermented type dietary fiber mainly include cellulose, lignin and some fermentation
Slow hemicellulose.Can not fermented type dietary fiber be that it can promote the propagation of microorganism to the Main Function of livestock and poultry,
So that microbial cleaning, degrade noxious material and reach the purpose of removing toxic substances, and some microorganisms can also suppress or slow down
The propagation of cancer cell；In addition, can not fermented type dietary fiber can also relax bowel, promote intestines peristalsis, reduce food in stomach and intestine
The dead time in road, reduce enteral pressure.The function of fiber：Used as a kind of special nutrient, it contains the bioactivity of uniqueness
Mixture of substances, including resistant starch, vitamin, mineral matter, phytochemicals and antioxidant content.
The content of small peptide substantially increases after feed fermentation.The Main Function of small peptide is that absorption is fast, is difficult saturation and amino
Acid has independent mechanism of absorption, contributes to mitigation free amino acid to vie each other and absorbs the antagonism of site generation jointly, also
The transhipment of free amino acid can be promoted, so as to promote Amino Acid Absorption to improve protein utilization rate.Small peptide has protection to digestion stage property
Effect, makes young animal small intestine precocious, the secretion of the enzyme that stimulates digestion.Small peptide has immunocompetence, can make to have in animal alimentary canal
Bacteria group is bred, and improves body's resistance to disease.
Oat bran crude protein only has 4%, and crude fiber content reaches 29%, moisture 7.2%, ash content 2.79, crude fibre
29.68%, it is a kind of extraordinary crude fibre raw material.But acid detergent fiber content is high, aflatoxin and vomitoxin
It is exceeded serious.
Oat bran also contains abundant fiber and other nutritional ingredients such as iron, potassium and vitamin B1 etc..Sent out by microorganism
After ferment, crude protein content increase 50-60%, iron, potassium and vitamin B1 equal size increase 10-15%, mycotoxin of effectively degrading,
Contain multiple-microorganism metabolite simultaneously, growth of animal can be promoted, strengthen disease resistance.
Citrus pulp is the product obtained with the remaining pulp of citrusfruit production canned fruit juice, fruit stone, pericarp crushed after being dried
Thing, color is in faint yellow or brown, there is light oranges and tangerines taste, tastes slightly bitter taste, and 90% be able to can be led to by 10 mesh standard sieves, 100%
Cross 8 mesh standard sieves.General component content is in citrus pulp：Dry 90.00%., wherein crude protein 9.8%, crude fat
3.7%, 30.6% crude fibre, NFE 60.3%, coarse ash 6.6%, calcium 1.84%, phosphorus 0.12%.In citrus pulp
Containing and have the material of bitter taste, have impact on effective utilization of animal.16 kinds of Bravo and DDVP residual etc. in citrus peel residue
Agricultural chemicals, the influence of residual content can effectively be degraded by fermentation, and improve the nutrient contents such as crude protein, significantly reduce thick fibre
Dimension hplc.
Citrus pulp is 13.79MJ/kg to the digestible energy of ruminant, and the digestible energy to pig is 13.78MJ/kg, to chicken
Metabolizable energy is 6.27MJ/kg.Citrus pulp arginine content 0.27%, lysine 0.22%, methionine 0.10%, cystine
0.12%, tryptophan 0.07%, iron is 378.0mg/kg, and copper 6.0mg/kg, manganese is 7.0mg/kg, and zinc is 15mg/kg.Citrus pulp
Nutritive value have very big change afterwards by fermentation, compared with unfermentable dry powder, thick protein improve 50%, amino acid is carried
High by 55%, crude fat improves 24%, and vitamin improves 10% one 60%, and crude fibre declines 17%, illustrates that fermentation can overcome the disadvantages that dry powder
The deficiency of protein, amino acid, and increase vitamin content, its nutrition is reached higher level, ferment 16 kinds of amino of citrus pulp
Acid content all increased total amino acid content increases to 10.84% from 7.59% respectively；Essential amino acids content increases
49.72%；Nonessential amino acid increases by 41.18% wherein lysine and increases by 80%；Methionine increases by 41.67%；Threonine increases
Plus 82.76%.To feeding cow, the corn flour of 40%-45%, and milk cow fur light, premunition enhancing can be replaced.
Clover is a kind of very excellent vegetable protein feed, and crude protein content is high, digestibility is high, rich in vitamin
With various mineral matters, more than 18%, feeding cow is remarkably improved the output of milk of milk cow to clover dry gross protein value
And milk quality, can also reduce because excessively eating the milk milk cow utilization periods long that fine fodder causes, increase economic efficiency.Research table
Bright, after undergoing microbial fermentation, Alfalfa crude fiber content is 11.90%, and 38.34%, crude protein are reduced during than not fermenting
Matter and true protein content are 29.62%, 22.97%, improve 42.41% and 28.97% when respectively than not fermenting, acid-soluble
The content of small peptide rises 12.59%, it is necessary to which amino acid and total amino acid content increased, and undergoing microbial fermentation can make lucerne
The gross protein value relative increase of Mu powder, reduces content of cellulose, acid-soluble peptide content is improved, so as to improve the feeding of alfalfa meal
Use quality.
Alfalfa meal is the extraordinary cellulose source of nutrition of pig chicken feed, wherein containing crude protein more than 20%, it is thick fine
Dimension more than 21%, the organic matter digestibility of pig only has 47%, and contains the harmful bacterias such as certain Escherichia coli and aspergillus flavus poison
The harmful substances such as element, after fermentation, improve the quantity of beneficial bacterium, reduce the quantity of harmful bacteria, are effectively improved pig chicken intestinal
Health status.
The method that Chinese invention application produces feed with stalk fermentation reduction crude fibre, application number：201410657067.2
The method that a kind of utilization stalk fermentation reduction crude fibre of disclosure of the invention produces feed, is related to agricultural technology field；Raw material preparation,
Stirring, pack, material are collected, add urea, modulation bacterium solution, pack, secondary fermentation, packaging etc.；The present invention can solve current straw
Stalk is not used as the low problem of feed stripped, stalk effective rate of utilization.
Compound bacteria-fermented high fiber agricultural byproducts produce the method for animal probiotics feed by utilizing, application number：
201110237021.1 disclose a kind of method that compound bacteria-fermented high fiber agricultural byproducts produce animal probiotics feed by utilizing, be by
The removal of impurities of high microsteping agricultural byproducts, pulverization process, with auxiliary material (0~6.0% ammonium sulfate, and/or 0~3.0% potassium dihydrogen phosphate and/
Or 0~1.0% calcium chloride and/or 0~1.0% magnesium sulfate) mix in proportion after moisten water steaming；It is cooled to after the completion of steaming
20~40 DEG C, while sturdy vein born of the same parents bacterium and Lactobacillus plantarum are inoculated with, 2~5d of solid state fermentation at 20~40 DEG C；Fermentation is completed
Feed be placed in 40 DEG C~70 DEG C crushed after being dried, obtain final product the probiotic feed product rich in carotenoid.This method has life
Efficiency high is produced, low cost, profitable advantage while solving problem of environmental pollution, alleviates energy and protein feed comes again
The pressure of source critical shortage.
The content of the invention
The raw material crude fiber content such as oat bran, citrus pulp and Alfalfa that the present invention is directed to is high, have mycotoxin contamination etc.
Situation, takes fermentation technique, increases the nutrient contents such as small peptide, reduces crude fiber content, increases the beneficial unknown factor, and improve
Beneficial bacterium quantity, improves the utilizing status of pig fowl.Composite fibre by fermentation can be used largely in pig feed, reduced into
This while, for pig provides safe feed guarantee.
In order to realize above-mentioned target, the scheme that the present invention is used is：
One kind is prepared for swine rearing mixed fermentation fiber feedstuff by the raw material of following parts by weight：
Citrus pulp, oat bran, 1 part of the saccharomycete mixed fermentation product A of Alfalfa, citrus pulp, oat bran, Alfalfa, vegetable
1-3 parts of the lactic acid bacteria mixed fermentation product B of dish, citrus pulp, oat bran, the bacillus mixed fermentation product C 2-3 of Alfalfa
Part.
The A is prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2.5-3:1-3 groups
Into raw mixture be added in heatable mixer, add water, the Portugal of 3-5% of raw mixture quality 40-60%
Grape sugar, after being heated to 70-80 DEG C of 20-30 minutes after being well mixed, 25-30 DEG C is cooled to by material, adds raw mixture matter
The mixed yeast seed liquor of 0.2-0.5% is measured, is cultivated 40-50 hours at 25-30 DEG C, speed of agitator is 20-30r/min, training
20-25 hours Cys of addition raw mixture quality 0.005-0.1%, the Radix Glycyrrhizae of 0.05-0.1% is supported to crush
Grain, uses fluidized bed drying after fermentation ends, obtain citrus pulp, oat bran, the yeast of Alfalfa of the moisture less than 14wt%
Bacterium mixed fermentation product A.
The B is prepared by following methods：By citrus pulp, oat bran, Alfalfa, vegetables according to mass ratio 1:2-3:1：
The raw mixture of 1 composition is added in container, add the water of raw mixture quality 30-70%, the glucose of 2-3%,
The mixing lactic acid bacteria seed liquor of 0.5-2%, is cultivated 50-75 hours at 25-37 DEG C, and fluidized bed drying is used after fermentation ends, is obtained
Citrus pulp, oat bran, Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables of the moisture less than 14wt%；The vegetables are
At least one of Chinese cabbage, tomato, lotus flower dish, cauliflower；The vegetables need to clean cutting to be used after fritter.
The C is prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2.5:1-4 groups
Into raw mixture be added in heatable mixer, add water, the Portugal of 2-3% of raw mixture quality 50-60%
Grape sugar, it is well mixed after be heated to 50-60 DEG C after 30 minutes, material is cooled to 25-30 DEG C, add raw mixture quality
The mixing sub- liquid of gemma bacillus specie of 0.1-0.3%, cultivates 50-80 hours at 25-30 DEG C, and speed of agitator is 30-50r/min.Hair
Ferment uses fluidized bed drying after terminating, and obtains citrus pulp of the moisture less than 14wt%, oat bran, the bacillus of Alfalfa and mixes
Close tunning C.
The mixed yeast seed liquor is prepared by following methods：By candida utili bacterium, S. cervisiae point
It is not inoculated in PDY fluid nutrient mediums, at 25-32 DEG C, is cultivated 30-40 hours under 150-200rpm, respectively obtains Candida utilis
Saccharomycete seed liquor, S. cervisiae seed liquor, then by candida utili bacterium seed liquor, S. cervisiae seed liquor according to body
Product compares 1:1~1:5 combined inoculations, at 25-32 DEG C, 20- are cultivated under 150-200rpm in mixed yeast seed liquid culture medium
30 hours, obtain mixed yeast seed liquor；The mixed yeast seed liquid culture medium is prepared by following methods：Albumen
Peptone 15.0g, glucose 18.0g, yeast extract 3.0g, sodium chloride 5.0g, distilled water 1000ml, pH value 6.8-7.2,121 DEG C
Sterilizing 20min；The saccharomyces cerevisiae is CGMCC No.12789.
The mixing lactic acid bacteria seed liquor is prepared by following methods：Lactobacillus plantarum, Pediococcus acidilactici are connect respectively
Plant in MRS fluid nutrient mediums, cultivated 20-30 hours under the conditions of 32-37 DEG C, respectively obtain Lactobacillus plantarum seed liquor, breast
Sour piece coccus seed liquor, then by Lactobacillus plantarum seed liquor, Pediococcus acidilactici seed liquor according to volume ratio 1:2~1:4 mixing connect
Plant in mixing lactic acid bacteria seed liquid culture medium, cultivated 20-30 hours at 30-37 DEG C, obtain mixing lactic acid bacteria seed liquor；Institute
Mixing lactic acid bacteria seed liquid culture medium is stated to be prepared by following methods：Peptone 12g, glucose 25g, beef extract 8g, yeast
Extract 6g, disodium hydrogen phosphate 5g, lemon acid diamine 0.25g, sodium acetate 6g, Tween 80 2ml, magnesium sulfate 0.8g, manganese sulfate
0.5g, ferrous sulfate 0.2g, distilled water 1000ml, pH value 6.0-6.5,120 DEG C of sterilizing 20min.
The mixing bacillus seed liquor is prepared by following methods：By bacillus subtilis, bacillus licheniformis
It is inoculated in respectively in TSB fluid nutrient mediums, is cultivated 12-30 hours under 30-37 DEG C, 150-250rpm, respectively obtains withered grass bud
Spore bacillus seed liquor, bacillus licheniformis seed liquor, then by bacillus subtilis seed liquor, bacillus licheniformis seed liquor according to
Volume ratio 1:1~1:3 combined inoculations to mix bacillus seed liquid culture medium in, under 30-37 DEG C, 150-250rpm cultivate
12-30 hours, obtain mixing bacillus seed liquor；The mixing sub- liquid culture medium of gemma bacillus specie is prepared into by following methods
Arrive：Soy peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate 4g, potassium chloride 2g, distilled water 1000ml, pH value
6.8-7.0,120 DEG C of sterilizing 20min.
It is a further object of the present invention to provide a kind of above-mentioned for the swine rearing preparation method of mixed fermentation fiber feedstuff.
In order to realize above-mentioned target, the scheme that the present invention is used is：
It is a kind of above-mentioned for the swine rearing preparation method of mixed fermentation fiber feedstuff, comprise the following steps：By citrus pulp,
The saccharomycete mixed fermentation product A of oat bran, Alfalfa, citrus pulp, oat bran, Alfalfa, the lactic acid bacteria mixed fermentation of vegetables
Product B, citrus pulp, oat bran, the bacillus mixed fermentation product C of Alfalfa are according to mass ratio 1:1-3:2-3 is well mixed
Obtain above-mentioned for swine rearing mixed fermentation fiber feedstuff.
Beneficial effect
Fermented by the mixing of citrus pulp, oat bran, Alfalfa and vegetables, as a kind of fermentable fiber feed applications in pig
Chicken feed can significantly improve pig, the intestinal villus development of chicken and Crypt depth, improve digestive function and intestinal health state；Fermentation
Composite fibre crude protein content of feed afterwards improves more than 40%, and the content of acid-soluble small peptide improves more than 12%, increases unknown
Trophic factors equal size, reduces crude fibre more than 30%, significantly improves the organic matter digestibility of feed, improves immunologic function and resists
Sick power, strong guarantee is provided to provide safe pig chicken product.In invention product be rich in saccharomycete, as probiotic group into
Divide and play a role, while the volume glutathione product that yeast fermentation is produced also is secreted into product.Increased product nutrients
Matter content, GSH contents reach 30mg/kg, improve the trophic function and effect of product.
Specific embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
On the premise of invention spirit and scope, the various changes that are carried out to the material component and consumption in these embodiments or change
Belong to protection scope of the present invention.
The following examples can make those skilled in the art that the present invention is more fully understood, but limit never in any form
The present invention.
CGMCC No.12789 are specially saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, the bacterial strain
In being preserved in the China Committee for Culture Collection of Microorganisms's common micro-organisms center of on July 15th, 2016, deposit number is
CGMCC No.12789, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica,
Postcode 100101.
The most suitable growth pH of the saccharomyces cerevisiae tlj2016 is 6.0-6.5, and optimum growth temperature is 28-35 DEG C；
The saccharomyces cerevisiae tlj2016 is isolated saccharomyces cerevisiae starting strain from the orchard of one plant of Ningxia through following
Step is obtained：
The original strain that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → hypertonic plate screening → nitrosoguanidine
(NTG) secondary screening (producing GSH abilities) → mitotic stability experiment is screened → fermented to mutagenesis screening → hypertonic flat board primary dcreening operation → shaking flask.
1st, saccharomyces cerevisiae provided by the present invention reaches 300g/L to the tolerance of glucose, beneficial to it in high concentration Portugal
GSH is produced under the conditions of grape sugar；
2nd, saccharomyces cerevisiae provided by the present invention reaches 3308mg/L in 5L fermentation cylinder for fermentation production GSH final concentrations；
3rd, the ability of saccharomyces cerevisiae tolerance Cys provided by the present invention is far above starting strain, in 5mmol/L
Slow growth is remained under Cys effect, remains to keep GSH largely to synthesize under the effect of 40mmol/LL- cysteines；
4th, saccharomyces cerevisiae salt resistance ability provided by the present invention reaches 18%, is conducive to extending its application field.
(1) GSH capacity experimentals are produced in tlj2016 fermentations under the conditions of sugar high
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Seed liquor is pressed into 10% inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput 6L/
Min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add final concentration
It is the Cys of 25mmol/L, total fermentation time is 50h；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0；
After fermentation ends, the content for determining GSH in zymotic fluid is 3308mg/L.
(2) Cys tolerance experiment
By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to
150rpm in shaking flask, 30 DEG C are cultivated, when culture is to 12h, to the Guang ammonia of L- half that different final concentrations are added in shaking flask
Acid, is further cultured for 10h, determines dry cell weight, as a result table 1,2；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 20, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
Table 1：Starting strain Cys tolerance
| Cys concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Starting strain dry weight g/L | 22.6 | 15.7 | 10.2 | 4.3 | 2.2 | 0.8 |
| GSH concentration (mg/L) | 35.6 | 46.7 | 43.2 | 40.7 | 37.9 | 25.3 |
Table 2：Tlj2016L- cysteine tolerances
| Cys concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Tlj2016 dry weights g/L | 25.7 | 28.5 | 23.6 | 21.2 | 20.6 | 18.7 |
| GSH concentration (mg/L) | 73.2 | 98.3 | 113.5 | 121.7 | 127.5 | 135.8 |
From the results shown in Table 2, for starting strain, Cys are added in culture medium, cell stops growing,
And start self-dissolving, cause GSH growth rates to be reduced with the rising of Cys concentration；And low concentration Cys
Under, tlj2016 still is able to slow growth, with the raising of Cys concentration, under the dry cell weight of tlj2016 bacterial strains is slow
Drop, and GSH concentration sustainable growths, this result is beneficial in GSH production processes by adding the Guang ammonia of precusor amino acids-L- half
Acid proposes the production for promoting GSH.
(3) salt resistance ability experiment
Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients be 0%, 2%, 5%,
10%th, 15%, 10mLYPD fluid nutrient mediums (pH=6.5) 18%), 24h is cultivated at being placed in 30 DEG C respectively, each treatment 3
Repeat.Respectively take 1ml samples bacterium solution to be mixed in 9ml physiological saline, prepare dilution factor solution, take 0.1ml dilutions in YPD solids
It is coated with flat board, culture 36 hours (each dilution factor do 3 parallel) record calculate flat board is inverted in 30 DEG C of biochemical cultivation cases
On bacterium number number.The results are shown in Table 3, it is known that the resistance to salinity of the bacterium is 18%, illustrates that tlj2016 not only can be in conventional environment
Middle existence, still has vigor under high salt conditions, can be applied to consume sugar product in the high salt food processing process such as soy sauce, curing food
Glutathione.
Table 3：Salt resistance ability detection (× 107cfu/ml)
Embodiment 1：
One kind is prepared for swine rearing mixed fermentation fiber feedstuff by following methods：By citrus pulp, oat bran,
The saccharomycete mixed fermentation product A of Alfalfa, citrus pulp, oat bran, Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables, mandarin orange
Tangerine slag, oat bran, the bacillus mixed fermentation product C of Alfalfa are according to mass ratio 1:2:2 well mixed obtain for pig
Mixed fermentation fiber feedstuff is used in raising.GSH contents reach 33mg/kg in the feed.
The preparation of A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2.8:The raw mixture of 2 compositions is added to
In heatable mixer, water, 4% glucose of raw mixture quality 50% are added, 75 DEG C are heated to after being well mixed
After 25 minutes, material is cooled to 28 DEG C, adds the mixed yeast seed liquor of raw mixture quality 0.4%, in 28 DEG C of trainings
Support 45 hours, speed of agitator is 25r/min, culture to 22 hours Cys of addition raw mixture quality 0.008%,
0.08% licorice powder particle, uses fluidized bed drying after fermentation ends, obtain citrus pulp, oat that moisture is 14wt%
The saccharomycete mixed fermentation product A of chaff, Alfalfa.
The preparation of B：By citrus pulp, oat bran, Alfalfa, vegetables according to mass ratio 1:2.5:1:The raw material mixing of 1 composition
Thing is added in container, adds water, 3% glucose, the 0.7% mixing lactic acid bacteria seed of raw mixture quality 60%
Liquid, is cultivated 65 hours at 28 DEG C, and fluidized bed drying is used after fermentation ends, obtains citrus pulp, oat that moisture is 14wt%
Chaff, Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables；The vegetables are Chinese cabbage, and the vegetables need to clean cutting for after fritter
Use.
The preparation of C：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2:The raw mixture of 3 compositions is added to can
In the mixer of heating, water, 2% glucose of raw mixture quality 55% are added, 55 DEG C 30 points are heated to after being well mixed
Zhong Hou, 26 DEG C are cooled to by material, add the mixing sub- liquid of gemma bacillus specie of raw mixture quality 0.2%, in 26 DEG C of cultures
70 hours, speed of agitator was 40r/min.After fermentation ends use fluidized bed drying, obtain moisture be 14wt% citrus pulp,
The bacillus mixed fermentation product C of oat bran, Alfalfa.
The preparation of mixed yeast seed liquor：Candida utili bacterium, S. cervisiae are inoculated in the training of PDY liquid respectively
Support in base, at 26 DEG C, cultivated 35 hours under 180rpm, respectively obtain candida utili bacterium seed liquor, S. cervisiae seed
Liquid, then by candida utili bacterium seed liquor, S. cervisiae seed liquor according to volume ratio 1:3 combined inoculations are to mixed yeast
In seed liquid culture medium, at 26 DEG C, cultivated 26 hours under 180rpm, obtain mixed yeast seed liquor；The mixed yeast
The preparation of seed liquid culture medium：Peptone 15.0g, glucose 18.0g, yeast extract 3.0g, sodium chloride 5.0g, distilled water
1000ml, 7,121 DEG C of sterilizing 20min of pH value；The S. cervisiae is CGMCC No.12789, the candida utili bacterium
It is CGMCC21641.
The preparation of mixing lactic acid bacteria seed liquor：Lactobacillus plantarum, Pediococcus acidilactici are inoculated in MRS fluid nutrient mediums respectively
In, cultivated 26 hours at 35 DEG C, respectively obtain Lactobacillus plantarum seed liquor, Pediococcus acidilactici seed liquor, then by plant breast bar
Bacterium seed liquor, Pediococcus acidilactici seed liquor are according to volume ratio 1:3 combined inoculations in mixing lactic acid bacteria seed liquid culture medium, 33
Cultivated 25 hours at DEG C, obtain mixing lactic acid bacteria seed liquor；The preparation of the mixing lactic acid bacteria seed liquid culture medium：Peptone
12g, glucose 25g, beef extract 8g, yeast extract 6g, disodium hydrogen phosphate 5g, lemon acid diamine 0.25g, sodium acetate 6g, tell
Warm 802ml, magnesium sulfate 0.8g, manganese sulfate 0.5g, ferrous sulfate 0.2g, distilled water 1000ml, 6.2,120 DEG C of sterilizings of pH value
20min；The Lactobacillus plantarum is CGMCC13919, and the Pediococcus acidilactici is CGMCC12696.
Mix the preparation of bacillus seed liquor：Bacillus subtilis, bacillus licheniformis are inoculated in TSB liquid respectively
In culture medium, cultivated 25 hours under 33 DEG C, 200rpm, respectively obtain bacillus subtilis seed liquor, bacillus licheniformis kind
Sub- liquid, then by bacillus subtilis seed liquor, bacillus licheniformis seed liquor according to volume ratio 1:2 combined inoculations to mix gemma
In bacillus seed liquid culture medium, cultivated 20 hours under 33 DEG C, 200rpm, obtain mixing bacillus seed liquor；The mixing
The preparation of bacillus seed liquid culture medium：Soy peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate 4g, chlorination
Potassium 2g, distilled water 1000ml, 6.8,120 DEG C of sterilizing 20min of pH value；The bacillus subtilis is CGMCC11700, lichens bud
Spore bacillus is CGMCC17461.
Embodiment 2
One kind is prepared for swine rearing mixed fermentation fiber feedstuff by following methods：By citrus pulp, oat bran,
The saccharomycete mixed fermentation product A of Alfalfa, citrus pulp, oat bran, Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables, mandarin orange
Tangerine slag, oat bran, the bacillus mixed fermentation product C of Alfalfa are according to mass ratio 1:1:3 it is well mixed obtain for
To for swine rearing mixed fermentation fiber feedstuff.GSH contents reach 32mg/kg in the feed.
The preparation of A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2.5:The raw mixture of 1 composition is added to
In heatable mixer, water, 3% glucose of raw mixture quality 60% are added, 70 DEG C are heated to after being well mixed
After 20 minutes, material is cooled to 25 DEG C, adds the mixed yeast seed liquor of raw mixture quality 0.2%, in 25 DEG C of trainings
Support 40 hours, speed of agitator is 20r/min, culture to 25 hours Cys of addition raw mixture quality 0.005%,
0.05% licorice powder particle, uses fluidized bed drying after fermentation ends, obtain citrus pulp, oat that moisture is 13wt%
The saccharomycete mixed fermentation product A of chaff, Alfalfa.
The preparation of B：By citrus pulp, oat bran, Alfalfa, vegetables according to mass ratio 1:2:1:The raw mixture of 1 composition
It is added in container, adds water, 3% glucose, the 0.5% mixing lactic acid bacteria seed liquor of raw mixture quality 30%,
Cultivated 50 hours at 25 DEG C, fluidized bed drying used after fermentation ends, obtain moisture be the citrus pulp of 13wt%, oat bran,
Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables；The vegetables are Chinese cabbage, tomato according to mass ratio 1:1 composition, it is described
Vegetables need to clean cutting to be used after fritter.
The preparation of C：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2.5:The raw mixture of 1 composition is added to
In heatable mixer, water, 2% glucose of raw mixture quality 50% are added, 50 DEG C are heated to after being well mixed
After 30 minutes, after material is cooled into 25 DEG C, the mixing sub- liquid of gemma bacillus specie of raw mixture quality 0.1% is added, 25
DEG C culture 80 hours, speed of agitator is 30r/min.Fluidized bed drying is used after fermentation ends, it is 13wt%'s to obtain moisture
Citrus pulp, oat bran, the bacillus mixed fermentation product C of Alfalfa.
The preparation of mixed yeast seed liquor：Candida utili bacterium, S. cervisiae are inoculated in the training of PDY liquid respectively
Support in base, at 25 DEG C, cultivated 40 hours under 150rpm, respectively obtain candida utili bacterium seed liquor, S. cervisiae seed
Liquid, then by candida utili bacterium seed liquor, S. cervisiae seed liquor according to volume ratio 1:1 combined inoculation is to mixed yeast
In seed liquid culture medium, at 25 DEG C, cultivated 30 hours under 150rpm, obtain mixed yeast seed liquor；The mixed yeast
The preparation of seed liquid culture medium：Peptone 15.0g, glucose 18.0g, yeast extract 3.0g, sodium chloride 5.0g, distilled water
1000ml, 6.8,121 DEG C of sterilizing 20min of pH value；The S. cervisiae is CGMCC No.12789, the candida utili
Bacterium is CGMCC21641.
The preparation of mixing lactic acid bacteria seed liquor：Lactobacillus plantarum, Pediococcus acidilactici are inoculated in MRS fluid nutrient mediums respectively
In, cultivated 30 hours at 32 DEG C, respectively obtain Lactobacillus plantarum seed liquor, Pediococcus acidilactici seed liquor, then by plant breast bar
Bacterium seed liquor, Pediococcus acidilactici seed liquor are according to volume ratio 1:2 combined inoculations in mixing lactic acid bacteria seed liquid culture medium, 30
Cultivated 30 hours at DEG C, obtain mixing lactic acid bacteria seed liquor；The preparation of the mixing lactic acid bacteria seed liquid culture medium：Peptone
12g, glucose 25g, beef extract 8g, yeast extract 6g, disodium hydrogen phosphate 5g, lemon acid diamine 0.25g, sodium acetate 6g, tell
Warm 802ml, magnesium sulfate 0.8g, manganese sulfate 0.5g, ferrous sulfate 0.2g, distilled water 1000ml, 6.0,120 DEG C of sterilizings of pH value
20min；The Lactobacillus plantarum is CGMCC13919, and the Pediococcus acidilactici is CGMCC12696.
Mix the preparation of bacillus seed liquor：Bacillus subtilis, bacillus licheniformis are inoculated in TSB liquid respectively
In culture medium, cultivated 30 hours under 30 DEG C, 150rpm, respectively obtain bacillus subtilis seed liquor, bacillus licheniformis kind
Sub- liquid, then by bacillus subtilis seed liquor, bacillus licheniformis seed liquor according to volume ratio 1:1 combined inoculation to mix gemma
In bacillus seed liquid culture medium, cultivated 30 hours under 30 DEG C, 150rpm, obtain mixing bacillus seed liquor；The mixing
The preparation of bacillus seed liquid culture medium：Soy peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate 4g, chlorination
Potassium 2g, distilled water 1000ml, 7,120 DEG C of sterilizing 20min of pH value；The bacillus subtilis is CGMCC11700, lichens gemma
Bacillus is CGMCC17461.
Embodiment 3
One kind is prepared for swine rearing mixed fermentation fiber feedstuff by following methods：By citrus pulp, oat bran,
The saccharomycete mixed fermentation product A of Alfalfa, citrus pulp, oat bran, Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables, mandarin orange
Tangerine slag, oat bran, the bacillus mixed fermentation product C of Alfalfa are according to mass ratio 1:3:2 it is well mixed obtain for
To for swine rearing mixed fermentation fiber feedstuff.GSH contents reach 32mg/kg in the feed.
The preparation of A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:3:The raw mixture of 2 compositions is added to can
In the mixer of heating, water, 5% glucose of raw mixture quality 40% are added, 80 DEG C 30 is heated to after being well mixed
After minute, material is cooled to 30 DEG C, adds the mixed yeast seed liquor of raw mixture quality 0.5%, in 30 DEG C of cultures
50 hours, speed of agitator was 30r/min, culture to 20 hours Cys of addition raw mixture quality 0.1%,
0.1% licorice powder particle, uses fluidized bed drying after fermentation ends, obtain moisture be the citrus pulp of 12wt%, oat bran,
The saccharomycete mixed fermentation product A of Alfalfa.
The preparation of B：By citrus pulp, oat bran, Alfalfa, vegetables according to mass ratio 1:3:1:The raw mixture of 1 composition
It is added in container, adds water, 2% glucose, the 2% mixing lactic acid bacteria seed liquor of raw mixture quality 70%,
37 DEG C are cultivated 75 hours, and fluidized bed drying is used after fermentation ends, obtain citrus pulp, oat bran, lucerne that moisture is 12wt%
Mu grass, the lactic acid bacteria mixed fermentation product B of vegetables；The vegetables are lotus flower dish, cauliflower according to mass ratio 2:1 composition, the vegetable
Dish need to clean cutting to be used after fritter.
The preparation of C：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1:The raw mixture of 4 compositions is added to can
In the mixer of heating, water, 3% glucose of raw mixture quality 60% are added, 60 DEG C 30 is heated to after being well mixed
After minute, after material is cooled into 30 DEG C, the mixing sub- liquid of gemma bacillus specie of raw mixture quality 0.3% is added, at 30 DEG C
Culture 50 hours, speed of agitator is 500r/min.Fluidized bed drying is used after fermentation ends, the mandarin orange that moisture is 12wt% is obtained
Tangerine slag, oat bran, the bacillus mixed fermentation product C of Alfalfa.
The preparation of mixed yeast seed liquor：Candida utili bacterium, S. cervisiae are inoculated in the training of PDY liquid respectively
Support in base, at 32 DEG C, cultivated 30 hours under 200rpm, respectively obtain candida utili bacterium seed liquor, S. cervisiae seed
Liquid, then by candida utili bacterium seed liquor, S. cervisiae seed liquor according to volume ratio 1:5 combined inoculations are to mixed yeast
In seed liquid culture medium, at 32 DEG C, cultivated 20 hours under 200rpm, obtain mixed yeast seed liquor；The mixed yeast
The preparation of seed liquid culture medium：Peptone 15.0g, glucose 18.0g, yeast extract 3.0g, sodium chloride 5.0g, distilled water
1000ml, 7.2,121 DEG C of sterilizing 20min of pH value；The S. cervisiae is CGMCC No.12789, the candida utili
Bacterium is CGMCC21641.
The preparation of mixing lactic acid bacteria seed liquor：Lactobacillus plantarum, Pediococcus acidilactici are inoculated in MRS fluid nutrient mediums respectively
In, cultivated 20 hours at 37 DEG C, respectively obtain Lactobacillus plantarum seed liquor, Pediococcus acidilactici seed liquor, then by plant breast bar
Bacterium seed liquor, Pediococcus acidilactici seed liquor are according to volume ratio 1:4 combined inoculations in mixing lactic acid bacteria seed liquid culture medium, 37
Cultivated 20 hours at DEG C, obtain mixing lactic acid bacteria seed liquor；The preparation of the mixing lactic acid bacteria seed liquid culture medium：Peptone
12g, glucose 25g, beef extract 8g, yeast extract 6g, disodium hydrogen phosphate 5g, lemon acid diamine 0.25g, sodium acetate 6g, tell
Warm 802ml, magnesium sulfate 0.8g, manganese sulfate 0.5g, ferrous sulfate 0.2g, distilled water 1000ml, 6.5,120 DEG C of sterilizings of pH value
20min；The Lactobacillus plantarum is CGMCC13919, and the Pediococcus acidilactici is CGMCC12696.
Mix the preparation of bacillus seed liquor：Bacillus subtilis, bacillus licheniformis are inoculated in TSB liquid respectively
In culture medium, cultivated 12 hours under 37 DEG C, 250rpm, respectively obtain bacillus subtilis seed liquor, bacillus licheniformis kind
Sub- liquid, then by bacillus subtilis seed liquor, bacillus licheniformis seed liquor according to volume ratio 1:3 combined inoculations to mix gemma
In bacillus seed liquid culture medium, cultivated 12 hours under 37 DEG C, 250rpm, obtain mixing bacillus seed liquor；The mixing
The preparation of bacillus seed liquid culture medium：Soy peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate 4g, chlorination
Potassium 2g, distilled water 1000ml, 7,120 DEG C of sterilizing 20min of pH value；The bacillus subtilis is CGMCC11700, lichens gemma
Bacillus is CGMCC17461.
Embodiment 4
By feed, common feed obtained in the present embodiment 1, using effect experiment is done.
Learnt by substantial amounts of gradient test result, mixed fermentation fiber feedstuff is best in the effect of baby pig feedstuff application 8%, because
The result of the test of this group profile of the present invention is addition 8%.
The result of comprehensive 11 experiments is as follows：The close weanling pig of 72 body weight of selection, is divided into 2 groups, including control group
With of the present invention group, every group of 6 repetitions, each repeat 6 pigs, experimental period be 35 days.
Table 4：Influence of the present invention to weaned piglets and intestinal tissue form
From table 4, present invention group average daily gain improves 17.9%, feedstuff-meat ratio reduction by 13.6%, Duodenal villi
Highly, ileum height of naps is respectively increased 13.8% and 10.2%, and it is notable that fermentable fiber feed improves intestine of young pigs health effect.
Table 5：Influence of the present invention to intestine of young pigs colony balance and thymic weight
From table 5, beneficial bacterium quantity is significantly improved in piglet chyme and in excrement, and immune organ weight is significantly improved.
Present invention using effect in terms of piglet is obvious, in industry-leading level.
Weanling pig is weaned the problem of syndrome, is also general character problem that global pig industry is faced.With state
The development of interior green additive biotechnology particularly probiotics technology, after solving the wean of piglet stage weanling pig
Intestinal health problem provides technical support.The application of of the present invention group of novel non-pollution feed of acquirement ideal effect in pig feed
Etc. can effectively substitute antibiotics use, suppress intestinal toxic microorganism species, regulating intestinal canal colony balance, enhancing is non-specific
Immunologic function etc., improves efficiency of feed utilization, ensures swinery health.
Present invention group fermentable fiber Feed Energy significantly reduces the coliform count in enteron aisle, re-forms enteric microorganism
Be conducive to the microecological balance of animal body and maintain normal physiological function；Beneficial bacterium lactic acid bacteria etc. can be harmful with competitive exclusion
Bacterium, prevents pathogen from positioning transfer, promotes alimentary canal reparation；Beneficial bacterium lactic acid bacteria etc. that fermented feed is produced can produce lactic acid,
Volatile fatty acid and protein and peptide antagonistic substance, so as to suppress the harmful microbe such as Escherichia coli growth and breeding and right
Invasive organism produces antagonism；The ratio of piglet height of naps and height of naps and Crypt depth after weaning can be improved；
Various digestive ferments are secreted simultaneously, improve the utilization rate of feed；Gut-associated lymphoid tissue can be promoted to develop, help to maintain this
A little lymphoid tissues are in the reaction " SBR " of height, improve antibody level, improve splenic T, the ratio of bone-marrow-derived lymphocyte, T, B
The increasing number of lymphocyte, reduces SAC, improves the humoral and cellular immune response level of animal body.
Of the present invention group improves piglet growth performance and Intestinal Morphology, and has front to gut flora and Immune Organs Index
Improve.
Claims (6)
1. one kind is for swine rearing mixed fermentation fiber feedstuff, it is characterised in that prepared by the raw material of following parts by weight：Mandarin orange
Tangerine slag, oat bran, 1 part of the saccharomycete mixed fermentation product A of Alfalfa, citrus pulp, oat bran, Alfalfa, the lactic acid bacteria of vegetables
1-3 parts of mixed fermentation product B, citrus pulp, oat bran, 2-3 parts of the bacillus mixed fermentation product C of Alfalfa.
2. it is according to claim 1 one kind for swine rearing mixed fermentation fiber feedstuff, it is characterised in that the A by
Following methods are prepared：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2.5-3:The raw mixture of 1-3 compositions
It is added in heatable mixer, adds water, the glucose of 3-5% of raw mixture quality 40-60%, after is well mixed
After being heated to 70-80 DEG C of 20-30 minutes, material is cooled to 25-30 DEG C, adds mixing for raw mixture quality 0.2-0.5%
Saccharomycete seed liquor is closed, is cultivated 40-50 hours at 25-30 DEG C, speed of agitator is 20-30r/min, culture added by 20-25 hours
Plus Cys, the licorice powder particle of 0.05-0.1% of raw mixture quality 0.005-0.1%, with stream after fermentation ends
Change bed to dry, obtain citrus pulp, oat bran, the saccharomycete mixed fermentation product A of Alfalfa of the moisture less than 14wt%.
3. it is according to claim 1 one kind for swine rearing mixed fermentation fiber feedstuff, it is characterised in that the B by
Following methods are prepared：By citrus pulp, oat bran, Alfalfa, vegetables according to mass ratio 1:2-3:1：The raw material mixing of 1 composition
Thing is added in container, adds water, the glucose of 2-3%, the mixed lactic of 0.5-2% of raw mixture quality 30-70%
Bacterium seed liquor, is cultivated 50-75 hours at 25-37 DEG C, and fluidized bed drying is used after fermentation ends, obtains moisture less than 14wt%
Citrus pulp, oat bran, Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables；
The vegetables are at least one of Chinese cabbage, tomato, lotus flower dish, cauliflower；The vegetables need to clean cutting to make after fritter
With.
4. it is according to claim 1 one kind for swine rearing mixed fermentation fiber feedstuff, it is characterised in that the C by
Following methods are prepared：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2.5:The raw mixture of 1-4 compositions
It is added in heatable mixer, adds water, the glucose of 2-3% of raw mixture quality 50-60%, after is well mixed
50-60 DEG C is heated to after 30 minutes, material is cooled to 25-30 DEG C, add the mixed bud of raw mixture quality 0.1-0.3%
Spore bacillus seed liquor, cultivates 50-80 hours at 25-30 DEG C, and speed of agitator is 30-50r/min；It is dry with fluid bed after fermentation ends
It is dry, obtain citrus pulp, oat bran, the bacillus mixed fermentation product C of Alfalfa of the moisture less than 14wt%.
5. one kind according to claim 2 is for swine rearing mixed fermentation fiber feedstuff, it is characterised in that the mixing
Saccharomycete seed liquor is prepared by following methods：Candida utili bacterium, S. cervisiae are inoculated in the training of PDY liquid respectively
Support in base, at 25-32 DEG C, cultivated 30-40 hours under 150-200rpm, respectively obtain candida utili bacterium seed liquor, wine brewing
Saccharomycete seed liquor, then by candida utili bacterium seed liquor, S. cervisiae seed liquor according to volume ratio 1:1~1:5 mixing
It is inoculated into mixed yeast seed liquid culture medium, at 25-32 DEG C, is cultivated 20-30 hours under 150-200rpm, obtains mixing ferment
Female bacterium seed liquor；
The mixed yeast seed liquid culture medium is prepared by following methods：Peptone 15.0g, glucose 18.0g, yeast
Extract 3.0g, sodium chloride 5.0g, distilled water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min；
The saccharomyces cerevisiae is CGMCC No.12789.
6. a kind of for the swine rearing preparation side of mixed fermentation fiber feedstuff according to claim 1-5 any one
Method, it is characterised in that comprise the following steps：By citrus pulp, oat bran, Alfalfa saccharomycete mixed fermentation product A, oranges and tangerines
Slag, oat bran, Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables, citrus pulp, oat bran, the bacillus mixing of Alfalfa
Tunning C is according to mass ratio 1:1-3:2-3 is well mixed to obtain above-mentioned for swine rearing mixed fermentation fiber feedstuff；
The preparation method of the A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2.5-3:The raw material mixing of 1-3 compositions
Thing is added in heatable mixer, adds water, the glucose of 3-5% of raw mixture quality 40-60%, is well mixed
After be heated to 70-80 DEG C of 20-30 minutes after, material is cooled to 25-30 DEG C, add raw mixture quality 0.2-0.5%'s
Mixed yeast seed liquor, cultivates 40-50 hours at 25-30 DEG C, and speed of agitator is 20-30r/min, and culture was by 20-25 hours
Cys, the licorice powder particle of 0.05-0.1% of addition raw mixture quality 0.005-0.1%, use after fermentation ends
Fluidized bed drying, obtains citrus pulp, oat bran, the saccharomycete mixed fermentation product A of Alfalfa of the moisture less than 14wt%；
The preparation method of the B：By citrus pulp, oat bran, Alfalfa, vegetables according to mass ratio 1:2-3:1：The raw material of 1 composition
Mixture is added in container, adds water, the mixing of the glucose, 0.5-2% of 2-3% of raw mixture quality 30-70%
Lactobacillus solution, is cultivated 50-75 hours at 25-37 DEG C, and fluidized bed drying is used after fermentation ends, is obtained moisture and is less than
The citrus pulp of 14wt%, oat bran, Alfalfa, the lactic acid bacteria mixed fermentation product B of vegetables；The vegetables be Chinese cabbage, tomato,
At least one of lotus flower dish, cauliflower；The vegetables need to clean cutting to be used after fritter；
The preparation method of the C：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2.5:The raw material mixing of 1-4 compositions
Thing is added in heatable mixer, adds water, the glucose of 2-3% of raw mixture quality 50-60%, is well mixed
After be heated to 50-60 DEG C after 30 minutes, material is cooled to 25-30 DEG C, add the mixing of raw mixture quality 0.1-0.3%
Bacillus seed liquor, cultivates 50-80 hours at 25-30 DEG C, and speed of agitator is 30-50r/min；Fluid bed is used after fermentation ends
Dry, obtain citrus pulp, oat bran, the bacillus mixed fermentation product C of Alfalfa of the moisture less than 14wt%；
The preparation method of the mixed yeast seed liquor：Candida utili bacterium, S. cervisiae are inoculated in PDY liquid respectively
In body culture medium, at 25-32 DEG C, under 150-200rpm cultivate 30-40 hours, respectively obtain candida utili bacterium seed liquor,
S. cervisiae seed liquor, then by candida utili bacterium seed liquor, S. cervisiae seed liquor according to volume ratio 1:1~1:5
Combined inoculation, at 25-32 DEG C, is cultivated 20-30 hours in mixed yeast seed liquid culture medium under 150-200rpm, is mixed
Close saccharomycete seed liquor；The mixed yeast seed liquid culture medium is prepared by following methods：Peptone 15.0g, grape
Sugared 18.0g, yeast extract 3.0g, sodium chloride 5.0g, distilled water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min；Institute
Saccharomyces cerevisiae is stated for CGMCC No.12789；
The preparation method of the mixing lactic acid bacteria seed liquor：Lactobacillus plantarum, Pediococcus acidilactici are inoculated in the training of MRS liquid respectively
Support in base, cultivated 20-30 hours under the conditions of 32-37 DEG C, respectively obtain Lactobacillus plantarum seed liquor, Pediococcus acidilactici seed
Liquid, then by Lactobacillus plantarum seed liquor, Pediococcus acidilactici seed liquor according to volume ratio 1:2~1:4 combined inoculations are to mixed lactic
In the sub- liquid culture medium of strain, cultivated 20-30 hours at 30-37 DEG C, obtain mixing lactic acid bacteria seed liquor；The mixing lactic acid bacteria
Seed liquid culture medium is prepared by following methods：Peptone 12g, glucose 25g, beef extract 8g, yeast extract 6g, phosphoric acid
Disodium hydrogen 5g, lemon acid diamine 0.25g, sodium acetate 6g, Tween 80 2ml, magnesium sulfate 0.8g, manganese sulfate 0.5g, ferrous sulfate
0.2g, distilled water 1000ml, pH value 6.0-6.5,120 DEG C of sterilizing 20min；
The preparation method for mixing bacillus seed liquor：Bacillus subtilis, bacillus licheniformis are inoculated in TSB respectively
In fluid nutrient medium, under 30-37 DEG C, 150-250rpm cultivate 12-30 hours, respectively obtain bacillus subtilis seed liquor,
Bacillus licheniformis seed liquor, then by bacillus subtilis seed liquor, bacillus licheniformis seed liquor according to volume ratio 1:1~1:
3 combined inoculations to mix bacillus seed liquid culture medium in, under 30-37 DEG C, 150-250rpm cultivate 12-30 hours, obtain
To mixing bacillus seed liquor；The mixing sub- liquid culture medium of gemma bacillus specie is prepared by following methods：Soybean protein
Peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate 4g, potassium chloride 2g, distilled water 1000ml, pH value 6.8-7.0,120 DEG C
Sterilizing 20min.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107183386A (en) * | 2017-06-21 | 2017-09-22 | 安徽和盛伟业饲料有限公司 | A kind of processing method of young pig green grass feed |
| CN108634112A (en) * | 2018-03-30 | 2018-10-12 | 山东丰沃新农农牧科技有限公司 | A kind of dietary fiber type religion guarantor's material |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1450168A (en) * | 2003-05-09 | 2003-10-22 | 江南大学 | Process for raising glutathion yield by fermentation of tornla yeast |
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN102860408A (en) * | 2012-09-24 | 2013-01-09 | 江西昌丰由由生物科技有限公司 | Preparation method of multifunctional live microorganism preparation for forage |
| CN104855678A (en) * | 2015-06-05 | 2015-08-26 | 沈玉平 | Method for producing tricin by utilizing viable bacteria glycolysis |
-
2016
- 2016-11-30 CN CN201611078963.9A patent/CN106721261A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1450168A (en) * | 2003-05-09 | 2003-10-22 | 江南大学 | Process for raising glutathion yield by fermentation of tornla yeast |
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN102860408A (en) * | 2012-09-24 | 2013-01-09 | 江西昌丰由由生物科技有限公司 | Preparation method of multifunctional live microorganism preparation for forage |
| CN104855678A (en) * | 2015-06-05 | 2015-08-26 | 沈玉平 | Method for producing tricin by utilizing viable bacteria glycolysis |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107183386A (en) * | 2017-06-21 | 2017-09-22 | 安徽和盛伟业饲料有限公司 | A kind of processing method of young pig green grass feed |
| CN108634112A (en) * | 2018-03-30 | 2018-10-12 | 山东丰沃新农农牧科技有限公司 | A kind of dietary fiber type religion guarantor's material |
| CN109043196A (en) * | 2018-08-28 | 2018-12-21 | 河南牧鹤(集团)饲料有限公司 | It is a kind of for improve fattening pig lean meat percentage feed |
| CN109156603A (en) * | 2018-09-29 | 2019-01-08 | 辽宁禾丰牧业股份有限公司 | A kind of compound premixed feed and preparation method thereof extending pork shelf life |
| CN109892474A (en) * | 2019-04-30 | 2019-06-18 | 陕西石羊农业科技股份有限公司 | One broad sow mixed feed and preparation method thereof |
| CN109907161A (en) * | 2019-04-30 | 2019-06-21 | 陕西石羊农业科技股份有限公司 | A kind of biological fermentation feed and preparation method thereof |
| CN110973353A (en) * | 2020-01-02 | 2020-04-10 | 四川农业大学 | Solid-state fermentation alfalfa meal feed and preparation method and application thereof |
| CN110973353B (en) * | 2020-01-02 | 2023-08-18 | 四川农业大学 | A kind of solid-state fermented alfalfa powder feed and its preparation method and application |
| CN111493234A (en) * | 2020-05-09 | 2020-08-07 | 乖宝宠物食品集团有限责任公司 | Fermented dietary fiber for promoting elimination of hair bulbs and growth of probiotics, and preparation method and application thereof |
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Application publication date: 20170531 |