CN102948621B - Prebiotic peptide biological feed additive and preparation method and application thereof - Google Patents

Abstract

The invention relates to a prebiotic peptide biological feed additive and its preparation method and application. The prebiotic peptide biological feed additive of the present invention is obtained by adding one or more seed liquids of Bacillus, yeast, and lactic acid bacteria to plant protease The solution is made by mixing and fermenting. After constant or variable temperature enzymolysis of animal and vegetable protein, one or more seed liquids of Bacillus, yeast, and lactic acid bacteria are added in a certain proportion for post-fermentation, and the fermented liquid is concentrated and dried to make a powder product. The prebiotic peptide feed additive solves the problems of high bitter peptide content and poor palatability caused by processes such as acid, alkali, and protease enzymatic hydrolysis. The active peptide in the prebiotic peptide biological feed additive of the present invention can stimulate the immune function of animals, improve the immunity of animals, and the beneficial bacteria can improve the intestinal environment of animals, enhance the resistance of animals to various diseases, and can improve the feed conversion rate, Reduced breeding costs.

Description

A kind of probiotic peptide biological feed additive and its preparation method and application

technical field

The invention relates to a prebiotic peptide biological feed additive and its preparation method and application, in particular to a biological feed additive containing active peptides and beneficial bacteria obtained by adding probiotics to the enzymatic hydrolyzate of animal and plant proteins for post-fermentation. It belongs to the field of biological feed.

Background technique

Since the 1950s, antibiotics have been used as growth promoters for livestock and poultry in the breeding industry. A large number of studies have shown that while antibiotics eliminate pathogenic bacteria, they also eliminate beneficial microorganisms to the animal body, disrupt the intestinal microecological balance, and cause animals, especially Susceptibility of young livestock and poultry to pathogenic microorganisms. In addition, long-term high-dose feeding of antibiotics can cause antibiotic-resistant bacteria harmful to both humans and animals in animals. At the same time, human consumption of animal products with residual antibiotics will also seriously affect human health.

In recent years, extensive research and experiments have been carried out on the substitutes of antibiotic growth promoters at home and abroad. Various natural products such as bioactive peptides, prebiotics, organic acids, prebiotics, enzyme preparations and Chinese herbal medicines have been used as antibiotics. Alternatives were tested with better results. Among the more alternatives to antibiotics, people pay more and more attention to the advantages of high efficiency, safety, and environmental protection with microecological preparations (prebiotics, probiotics) or biofermented feed fermented with microorganisms.

Probiotics, also known as prebiotics or probiotics, are defined by the Food and Drug Administration of the United States as Direct-Fed Microbes (DFM), that is, "living natural microorganisms". In the livestock and aquaculture industry, probiotics have been widely used in production practice, and can increase the growth rate of livestock and poultry, reduce the incidence of young livestock, and can partially replace the growth-promoting and disease-preventing functions of antibiotics.

Bioactive peptides (or oligopeptides) refer to a class of polypeptides with a molecular weight less than 3000D, a loose conformation, and multiple biological functions. It has the characteristics of easy digestion and absorption, anti-fatigue, lowering blood pressure, lowering cholesterol, regulating insulin action, promoting mineral absorption, promoting fat metabolism, promoting microbial fermentation and low allergenicity.

In recent decades of research, animal nutritionists have found that the utilization of various amino acids in feed by animals is not completely affected by the level of a single limiting amino acid, nor does it fully follow the "bucket rule" in nutrition. classical theory. In addition, animals were not optimally productive when fed homozygous diets formulated with ideal amino acid patterns or low-protein amino acid balanced diets. In the 1950s and 1960s, Newey and Smyth first proposed the evidence of complete absorption, but until the 1980s, due to the continuous accumulation of direct and indirect experiences, the view of complete absorption of peptides and its physiological effects were not taken seriously. Its related research has also become unprecedentedly active, and many research results have been obtained.

Application of Bioactive Peptides in Animal Production

1. Improve feed palatability

Certain peptides can promote the four tastes of sweet, sour, bitter, and salty. For example: Aspartame is a highly sweetening peptide, its sweetness is 100 to 200 times that of sucrose, and it has been approved to be used in more than 500 kinds of food and medicine by more than 70 countries; some peptides are also used as fish Chemical attractant for feed. Can be designed and used to improve palatability by simulating, masking or enhancing taste.

2. Improve animal production performance

After digestion, protein does not have to be degraded into completely free amino acids to be absorbed, but is mainly absorbed in the form of small peptides. The absorption rate of small peptides is higher than that of amino acids, and it is easier and faster to be absorbed and utilized by the body than amino acids. At the same time, the low antigenicity of small peptides will not cause allergic reactions after eating; and the osmotic pressure of small peptides is lower than that of amino acids, and will not cause adverse reactions such as dysentery after eating.

3. Promote bone growth and improve eggshell quality

4. Other functions

By reducing the protein content in the feed, the use of small peptides of 2-50 amino acid residue fragments to replace protein can further optimize animal production, reduce a large amount of nitrogen in animal manure, and reduce pollution to lakes, rivers and groundwater.

Contents of the invention

The purpose of the present invention is to provide a production method of prebiotic peptide biological feed additive and the feed additive prepared by the method. In the present invention, when the animal and plant protein is enzymatically hydrolyzed to 70-90% of the acid-soluble protein content, the temperature is raised to kill the enzyme, followed by One or more seed liquids of spores, yeasts, and lactic acid bacteria are added for post-fermentation, and the fermented liquid is concentrated, dried, and granulated to form a new type of biological feed additive containing beneficial bacteria and bioactive peptides. The biological feed additive can replace antibiotics and be used in breeding and feed industry.

The purpose of the present invention is achieved through the following technical solutions:

A method for preparing a prebiotic peptide biological feed additive of the present invention is characterized in that the method comprises enzymolyzing the animal or plant protein after high-temperature cooking until the content of acid-soluble protein is 30-90% by mass, and then raising the temperature Inactivate the enzyme, lower the temperature, then insert one or more of the seed liquids of bacillus, yeast or lactic acid bacteria for mixed fermentation, and finally concentrate, dry and granulate the fermented liquid.

In the present invention, preferably, the animal protein includes: fresh blood, meat or internal organs of various animals;

The vegetable protein includes soybean protein, wheat protein, corn protein, rapeseed protein and cottonseed protein.

In the present invention, preferably, the bacillus includes one or more of Bacillus subtilis, Bacillus licheniformis and Bacillus natto;

The yeast includes one or more of Saccharomyces cerevisiae and Candida utilis;

The lactic acid bacteria include one or more of Lactobacillus casei, Lactobacillus plantarum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus bulgaricus and Lactobacillus helveticus.

In the present invention, preferably, the high-temperature cooking refers to adding water to animal or vegetable protein and stirring and heating at 95°C-120°C for 30-60 minutes (animal and vegetable protein must be cooked at high temperature to expand and absorb water, and the inside becomes Soft, which is conducive to the progress of enzymatic hydrolysis.), the animal or vegetable protein after high-temperature cooking is ground by colloid mill to a particle size of 100-200 mesh, and then heated by a plate heat exchanger and then pumped to the enzymatic hydrolysis tank for protease enzymolysis.

In the present invention, preferably, said protease includes papain, bromelain, neutral protease, alkaline protease, trypsin and various animal and plant proteolytic enzymes, and said hydrolytic enzymes include endoproteinase, exoproteinase Cut enzyme, flavor enzyme.

In the present invention, preferably, the temperature raising to kill the enzyme is to raise the temperature of the enzymolysis solution to 85-90° C. and keep the temperature constant for 30-60 minutes after the end of the enzymolysis.

In the present invention, preferably, the inoculation process includes adding sterilized fermentation medium according to the ratio of the volume ratio of the enzymolysis solution and the fermentation medium to 1.5:1, and then making Bacillus, yeast, and lactic acid bacteria into corresponding Seed solution, when the temperature of the enzymolysis solution drops to 25-40°C, according to the volume percentage, add 10%-15% of Bacillus, yeast or lactic acid bacteria or a mixture of several seed solutions. When different seed liquids are used, the volume ratio of the two seed liquids is 1-2:1-2. When three different seed liquids are used, the volume ratio of the three seed liquids is 1-2:0.5-2:1 -2, more preferably, the volume ratio of the three kinds of seed liquids added is 1:1:1, the constant temperature is 25-40°C, after inoculation, sterile air is introduced at a speed of 1.0-3.5vvm, and mixed fermentation is carried out for 48-96h;

Wherein said fermentation medium is prepared according to the following method:

Weigh 5.0g of soybean peptone; 5.0g of glucose; 5.0g of yeast extract powder; 4.0g of potassium dihydrogen phosphate; 20 minutes, set aside.

In the present invention, preferably, the concentration is concentrated by thin film, that is, concentrated by a downstream double-effect falling film concentrator until the dry matter content is 15-60%. 0.90～0.95Mpa; Second effect temperature 70～80℃, vacuum degree 0.80～0.85Mpa.

In the present invention, preferably, the drying and granulation is to dry the concentrated fermented liquid by circulating hot air at 70-80°C, or directly spray-dry the concentrated liquid into powder by vacuum spray drying, add a carrier, and take It is granulated by low-temperature granulation, and the moisture content of the finished granules is 8-12%.

Furthermore, the present invention also provides the prebiotic peptide biological feed additive prepared according to any one of the above methods.

Furthermore, the present invention also provides the application of the prebiotic peptide biological feed additive in the preparation of animal feed.

The invention provides a production method of a prebiotic peptide biological feed additive. The prebiotic peptide provided by the invention is compounded by a beneficial bacterium and an active peptide, and has dual biological effects of the probiotic and the active peptide. The bacterial strains and their secretions in this method are safe and harmless to humans and animals, and can be well expressed on protein substrates. The bacterial strains can secrete suitable proteases to cut proteins into suitable lengths of peptides in vitro. Post-fermentation of beneficial bacteria can better remove anti-nutritional factors and some protein antigen components in protein raw materials, and because lactic acid bacteria, yeast, bacillus and other strains are added in the microbial fermentation process, it can also produce about 3% lactic acid and fermentation aroma, It has the function of acidifier, which is beneficial to increase the feed intake of animals and improve the microenvironment of animal gastrointestinal tract.

Detailed ways

The present invention will be further described through experiments and examples below. It should be understood that these examples are only for the purpose of illustration, and in no way limit the protection scope of the present invention.

The preparation of embodiment 1 fermentation medium

Soy peptone 5.0g; glucose 5.0g; yeast extract powder 5.0g; potassium dihydrogen phosphate 4.0g; 3.08% (w/w) manganese sulfate solution 1mL, deionized water 1000ml, pH 6.50. .

The preparation of embodiment 2 seed liquid

1. Purchase source of the strains involved: All strains were purchased from the China Industrial Microbiology Culture Collection Center, and the specific numbers of the strains are:

Bacillus subtilis CICC 10066, Bacillus licheniformis CICC 10037, Bacillus natto CICC10260, Saccharomyces cerevisiae CICC 1562, Candida utilis CICC 1314, Lactobacillus casei CICC 20266, Lactobacillus plantarum CICC 20242, Lactobacillus acidophilus CICC 21000, Streptococcus thermophilus CICC 20364, Lactobacillus bulgaricus CICC 20247, Lactobacillus helveticus CICC 22826.

2. Preparation of seed solution

A: Bacillus subtilis and Bacillus licheniformis seed liquid

1) Bacillus culture medium

Soy peptone 5.0g; glucose 5.0g; yeast extract powder 5.0g; potassium dihydrogen phosphate 4.0g; 3.08% (w/w) manganese sulfate solution 1mL, deionized water 1000ml, pH 6.50. .

2) Seed solution preparation:

The sterilized culture medium was inoculated with Bacillus subtilis or Bacillus licheniformis according to 2% inoculum amount (w/w), cultured in shake flask at 30°C for 24 hours, and set aside.

B: Candida utilis and Saccharomyces cerevisiae seed solutions:

1) Yeast medium:

Soybean peptone 5.0g; glucose 10.0g; yeast extract powder 3.0g; malt extract 3.0g, deionized water 1000ml, pH 6.00.

2) Seed solution preparation:

The sterilized culture medium was inoculated with Candida utilis or Saccharomyces cerevisiae at a 2% (w/w) inoculum amount, cultured at 30°C for 48 hours, and set aside.

C: Streptococcus thermophilus seed solution

1) Medium for Streptococcus thermophilus:

Polypeptone (or tryptone) 5g, plant peptone 5g, beef extract 5g, yeast extract 2.5g, β-glycerol disodium phosphate 19g, ascorbic acid 0.5g, MgSO ₄ 7H ₂ O 0.25g, lactose 5g, distilled water 1000mL, pH7.1, 0.07MPa sterilization for 20min.

2) Seed solution preparation:

The sterilized culture medium was inoculated with Streptococcus thermophilus at a 2% (w/w) inoculum size, cultured at 37°C for 24 hours, and set aside.

D: Lactobacillus (Lactobacillus casei, Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus helveticus) seed solution

1) Lactobacillus culture medium:

Peptone 5.0g; beef extract 5.0g; tryptone 10.0g; yeast powder 5.0g; glucose 10.0g; Tween-80 1.0g; K ₂ HPO ₄ 2.0g; sodium acetate 5.0g; ₄ ·7H ₂ O 0.25g; MgSO ₄ ·7H ₂ O 0.1g; deionized water 1000mL; pH 6.20, 0.07MPa sterilization for 20min.

2) Seed solution preparation:

The sterilized medium was inoculated with Lactobacillus casei, Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus bulgaricus or Lactobacillus helveticus at a 2% (w/w) inoculation amount, cultured at 37°C for 24 hours, and set aside.

Example 3 Preparation of Enzymolysis Pig Blood Prebiotic Peptide Biological Feed Additive

1) Pre-treatment of pig blood

Crush the coagulated fresh pig blood, add water at a ratio of 1:10, raise it to 115°C, keep the temperature at a constant temperature for 60 minutes, and keep stirring during the period, then grind it through a colloid mill, adjust the colloid mill to 200 mesh, and use a thick slurry The pump heats the ground pig blood through a plate heat exchanger to 58°C and then pumps it into the enzymolysis tank.

2) Enzymolysis

Add alkaline protease (2000U/g) at a weight ratio of 1:250 between protease and treated pig blood, pH 8.5, constant temperature 50°C, start stirring, aseptically take samples every 4 hours to measure the degree of proteolysis, When the acid-soluble protein content is greater than 70% (w/w), proceed to the next step.

3) Warm up to kill enzymes

Heat the enzymolysis solution to 85°C and keep the temperature constant for 60 minutes to inactivate the protease in it.

4) Vaccination

When the temperature of the enzymolysis solution drops to 28°C, first add the sterilized fermentation medium (prepared in Example 1), the volume ratio of the enzymolysis solution to the fermentation medium is 1.5:1, and then add 10% (volume ratio) Enter Bacillus subtilis, Candida utilis, Streptococcus thermophilus (or Lactobacillus plantarum) seed solution (prepared in Example 2). Keep the temperature at 30°C, pass sterile air at a rate of 2.0vvm after inoculation, and mix and ferment for 72h.

The ratio of Bacillus subtilis, Candida utilis, Streptococcus thermophilus (or Lactobacillus plantarum) seed solution is 1:1:1.

The age of each fermentation strain is as follows:

Bacillus subtilis 24h, Candida utilis 48h, Streptococcus thermophilus (or Lactobacillus plantarum) 24h.

5) Concentration and drying

Vacuum film concentration: Concentrate to a dry matter content of 20% through a high-efficiency downstream falling film vacuum concentrator. When concentrating, the temperature of the first effect is 80°C and the degree of vacuum is 0.95Mpa; the temperature of the second effect is 70°C and the degree of vacuum is 0.80Mpa.

After vacuum concentration, it was dried with circulating hot air at 75°C. The concentrate is directly spray-dried into powder by means of vacuum spray drying, and the water content of the finished product is 8%.

Example 4 Preparation of Enzymatically Hydrolyzed Chicken Blood Prebiotic Peptide Biological Feed Additive

1) Pre-treatment of chicken blood

Crush the coagulated fresh chicken blood, add water at a ratio of 1:10, then raise it to 120°C, keep the temperature at a constant temperature for 45 minutes, and keep stirring during the period, then grind it through a colloid mill, adjust the colloid mill to 200 mesh, and use a thick slurry The pump heats the ground blood to 50°C through a plate heat exchanger and then pumps it into the enzymolysis tank.

2) Enzymolysis

Add alkaline protease (2000U/g) at a weight ratio of protease to chicken blood of 1:300, pH 8.5, constant temperature 50°C, start stirring, take aseptic samples every 4 hours to measure the degree of protein hydrolysis, when the acid dissolves Proceed to the next step when the protein content is greater than 70% (w/w).

3) Warm up to kill enzymes

Heat the enzymolysis solution to 75°C and keep the temperature constant for 60 minutes to inactivate the protease in it.

4) Vaccination

When the temperature of the enzymolysis solution drops to 30°C, first add the sterilized fermentation medium (prepared in Example 1), the volume ratio of the enzymolysis solution to the fermentation medium is 1.5:1, and then add 15% (volume ratio) Add Bacillus subtilis, Candida utilis, Streptococcus thermophilus (or Lactobacillus plantarum) seed solution (prepared in Example 2), keep the temperature at 30°C, pass sterile air at a speed of 2.0vvm after inoculation, and mix and ferment for 60h .

The ratio of Bacillus subtilis, Candida utilis, Streptococcus thermophilus (or Lactobacillus plantarum) seed solution is 1:1:2.

The age of each fermentation strain is as follows:

Bacillus subtilis 36h, Candida utilis 48h, Streptococcus thermophilus 24h.

5) Concentration and drying

Vacuum thin film concentration: Concentrate to a dry matter content of 30% through a downstream double-effect falling film vacuum concentrator. When concentrating, the temperature of the first effect is 85°C and the degree of vacuum is 0.95Mpa; the temperature of the second effect is 75°C and the degree of vacuum is 0.80Mpa.

After vacuum concentration, it was dried with circulating hot air at 60°C. The concentrate is directly spray-dried into powder by means of vacuum spray drying, and the water content of the finished product is 12%.

Example 5 Preparation of Enzymolysis Pork Prebiotic Peptide Biological Feed Additive

1) Pre-treatment of pork

Wash the pork to remove the blood, cut off the oil, chop and mix it into a meat paste, add water at a ratio of 1:4, raise the temperature to 95°C, keep it at a constant temperature for 45 minutes, and keep stirring during the period, and then grind it through a colloid mill. Adjust to 200 mesh, pump the feed liquid to the enzymolysis tank after heating to 50°C.

2) Enzymolysis

Add protease at 1.5% of the weight of the minced meat, the protease is composed of papain (8.0×10 ⁵ IU/g) and neutral protease (2.3×10 ⁵ IU/g) in a ratio of 1:2, pH 8.0, constant temperature 50°C , start stirring, and proceed to the next step when the acid-soluble protein content reaches 30% (w/w) or more after hydrolysis for 4 hours.

3) Warm up to kill enzymes

Heat the enzymolysis solution to 85°C and keep the temperature constant for 60 minutes to inactivate the protease in it.

4) Vaccination

When the temperature of the enzymolysis solution drops to 28°C, first add the sterilized fermentation medium (prepared in Example 1), the volume ratio of the enzymolysis solution and fermentation medium is 1.5:1, and then add 10% (volume ratio) Bacillus subtilis, Candida utilis, Lactobacillus casei (or Lactobacillus helveticus or Lactobacillus acidophilus) seed solution (prepared in Example 2). Keep the temperature at 30°C, pass sterile air at a rate of 2.0vvm after inoculation, and mix and ferment for 72h.

The ratio of Bacillus subtilis, Candida utilis, Lactobacillus casei (or Lactobacillus helveticus or Lactobacillus acidophilus) seed solution is 1:1:1.

The age of each fermentation strain is as follows:

Bacillus subtilis 24h, Candida utilis 48h, Lactobacillus casei (or Lactobacillus helveticus or Lactobacillus acidophilus) 24h.

5) Concentration and drying

Vacuum film concentration: Concentrate to a dry matter content of 20% through a high-efficiency downstream falling film vacuum concentrator. When concentrating, the temperature of the first effect is 80°C and the degree of vacuum is 0.95Mpa; the temperature of the second effect is 70°C and the degree of vacuum is 0.80Mpa.

After vacuum concentration, it was dried with circulating hot air at 75°C. The concentrate is directly spray-dried into powder by means of vacuum spray drying, and the water content of the finished product is 10%.

Example 6 Preparation of Enzymolysis Fish Meat Prebiotic Peptide Biological Feed Additive

1) Pre-treatment of fish meat or viscera

Wash the fish meat or viscera to remove blood, chop and mix into meat paste, add water at a ratio of 1:3, raise the temperature to 95°C, keep at a constant temperature for 30 minutes, and keep stirring during the period, then grind it through a colloid mill, and adjust the colloid mill to 200 The feed liquid is heated to 50°C and then pumped into the enzymolysis tank. After stirring evenly, pump it into the enzymatic hydrolysis tank.

2) Enzymolysis

Add alkaline protease according to the enzyme amount of 2000U/g, pH 9.0, constant temperature 50°C, start stirring, and proceed to the next step when the acid-soluble protein content reaches 40% (w/w) or more after hydrolysis for 7.5 hours.

3) Warm up to kill enzymes

Heat the enzymolysis solution to 95°C and keep the temperature for 15 minutes to inactivate the protease in it.

4) Vaccination

When the temperature of the enzymolysis solution drops to 28°C, first add the sterilized fermentation medium (prepared in Example 1), the volume ratio of the enzymolysis solution and fermentation medium is 1.5:1, and then add 10% (volume ratio) Bacillus subtilis, Candida utilis, Lactobacillus casei (or Lactobacillus bulgaricus) seed solution (prepared in Example 2). Keep the temperature at 30°C, pass sterile air at a rate of 2.0vvm after inoculation, and mix and ferment for 72h.

The ratio of Bacillus subtilis, Candida utilis, Lactobacillus casei (or Lactobacillus bulgaricus) seed solution is 1:1:1.

The age of each fermentation strain is as follows:

Bacillus subtilis 24h, Candida utilis 48h, Lactobacillus casei (or Lactobacillus bulgaricus) 24h.

5) Concentration and drying

Vacuum film concentration: Concentrate to a dry matter content of 20% through a high-efficiency downstream falling film vacuum concentrator. When concentrating, the temperature of the first effect is 80°C and the degree of vacuum is 0.95Mpa; the temperature of the second effect is 70°C and the degree of vacuum is 0.80Mpa.

After vacuum concentration, it was dried with circulating hot air at 75°C. The concentrate is directly spray-dried into powder by means of vacuum spray drying, and the water content of the finished product is 10%.

Example 7 Preparation of Enzymatically Hydrolyzed Soybean Meal Prebiotic Peptide Biological Feed Additive

1) Pre-treatment of soybean meal

Crush the soybean meal, add water at a ratio of 1:4, raise the temperature to 95°C, keep at a constant temperature for 30 minutes, and keep stirring during the period, then grind it through a colloid mill, adjust the colloid mill to 200 mesh, heat the feed liquid to 50°C, and then pump into the enzymatic tank. After stirring evenly, pump it into the enzymatic hydrolysis tank.

2) Enzymolysis

Add alkaline protease according to the enzyme amount of 4000U/g, pH 8.0, constant temperature 50°C, start stirring, and proceed to the next step when the acid-soluble protein content reaches more than 30% (w/w) after hydrolysis for 6 hours. .

3) Warm up to kill enzymes

Raise the temperature of the enzymolysis solution to 85°C and keep the temperature constant for 15 minutes to inactivate the protease in it.

4) Vaccination

When the temperature of the enzymolysis solution drops to 28°C, first add the sterilized fermentation medium (prepared in Example 1), the volume ratio of the enzymolysis solution to the fermentation medium is 1.5:1, and then add 10% (volume ratio) Bacillus subtilis (or Bacillus licheniformis), Candida utilis (or Saccharomyces cerevisiae), and Lactobacillus plantarum (or Lactobacillus helveticus) seed solution (prepared in Example 2). Keep the temperature at 30°C, pass sterile air at a rate of 2.0vvm after inoculation, and mix and ferment for 72h.

The seed liquid ratio of Bacillus subtilis (or Bacillus licheniformis), Candida utilis (or Saccharomyces cerevisiae), and Lactobacillus plantarum (or Lactobacillus helveticus) seed liquid is 1:1:1.

The age of each fermentation strain is as follows:

Bacillus subtilis (or Bacillus licheniformis) 24h, Candida utilis (or Saccharomyces cerevisiae) 48h, Lactobacillus plantarum (or Lactobacillus helveticus) 24h.

5) Concentration and drying

Vacuum film concentration: Concentrate to a dry matter content of 20% through a high-efficiency downstream falling film vacuum concentrator. When concentrating, the temperature of the first effect is 80°C and the degree of vacuum is 0.95Mpa; the temperature of the second effect is 70°C and the degree of vacuum is 0.80Mpa.

After vacuum concentration, it was dried with circulating hot air at 75°C. The concentrate is directly spray-dried into powder by means of vacuum spray drying, and the water content of the finished product is 8%.

Example 8 Application of the prebiotic peptide feed additive of the present invention in improving feed utilization and improving animal production performance

Select 300 healthy, lively, 12-day-old female broilers of similar weight, and divide them into two groups randomly, 145 in the control group and 155 in the test group. 3 repetitions per set. Diet treatment: group A is the control group, fed with basal diet; group B is basal diet + 2% (w/w) prebiotic peptide feed additive (according to the method described in Example 3 by inoculating Bacillus subtilis, producing Candida prion and Streptococcus thermophilus) were fed continuously for 35 days. The broiler production performance is shown in Table 1.

Table 1 Effects of prebiotic peptide feed additives on initial weight, final weight, and daily gain of broilers

It can be seen from the above table that the addition of prebiotic peptide feed additives in the feed can greatly promote the improvement of feed utilization rate and animal production performance. After 35 days of feeding experiment, the average daily gain of chickens in the test group was 35.04g/d , 2.56g higher than that of the control group. The feed intake of the chickens in the test group was lower than that in the control group, and the feed-to-meat ratio was lower.

A total of 46 weaned Du and Sanyuan piglets were selected for the experiment and randomly divided into two groups, including 23 pigs in the test group and 23 pigs in the control group. The two groups were reared uniformly by special personnel using the same feeding method. The test group used the farm piglet base material + 5% (w/w) prebiotic peptide feed additive (prepared by inoculating Bacillus subtilis, Candida utilis and Streptococcus thermophilus according to the method described in Example 3), and the control group The group used the piglet basic feed, and recorded the feed amount and feed waste of each group of piglets. Each head was weighed on an empty stomach before and after the experiment. Statistics of feed consumption, calculation of daily weight gain and feed-to-meat ratio. The test is 15 days in total.

1) Effect of prebiotic peptide feed additive on diarrhea

At the beginning of the experiment, some of the piglets in the two groups had diarrhea, but the piglets in the test group no longer had diarrhea after 5 days, and a small part of the piglets in the control group had diarrhea during the whole test process, so adding 5% active peptide in the feed additive Bacteria have been shown to be effective in improving gut health.

2) Effect of prebiotic peptide feed additive on average daily gain

The average daily gain of the test group was 326g, and the average daily gain of the control group was 267g. The daily gain of the test group was 59g more than that of the control group, an increase of 22%. The difference was extremely significant (P<0.01), see Table 2.

Table 2 Effects of prebiotic peptide feed additives on the initial weight, final weight, and daily gain of piglets (kg)

The feed-to-meat ratio of the test group was 1.70, and that of the control group was 2.08, which was 0.38 lower than that of the control group, which significantly improved the feed utilization rate (P<0.01).

The above are only preferred embodiments of the present invention, and do not limit the present invention in any form. Any modifications, equivalent changes and modifications made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still belong to within the scope of the technical solutions of the present invention.

Claims (8)

1. the preparation method of a prebiotic peptide biology feed additive, after it is characterized in that described method comprises that extremely the content of sour molten albumen is mass percent 30～90% through protease hydrolyzed by the animal or plant protein after thermophilic digestion, the intensification enzyme that goes out, cooling, then access bacillus, saccharomycete and lactobacillus solution and carry out mixed culture fermentation, finally zymotic fluid is concentrated, dry, granulation obtains;

Wherein, described thermophilic digestion refers to animal or plant protein is added to water agitating heating 30-60 minute at 95 ℃-120 ℃, animal or plant protein after thermophilic digestion, by colloid mill, be milled to granular size 100～200 orders, after then heating by plate type heat exchanger, pump carries out protease hydrolyzed to enzymatic vessel;

Wherein, seeded process comprises the fermentation medium that adds sterilizing in the ratio of the volume ratio 1.5:1 of enzymolysis liquid and fermentation medium, then bacillus, saccharomycete, lactic acid bacteria culturers are made to corresponding seed liquor, when enzymolysis liquid temperature is down to 25～40 ℃, according to percent by volume, calculate, the mixed liquor that adds bacillus, saccharomycete and the lactobacillus solution of 10%-15%, the volume ratio that adds of three kinds of seed liquor is 1:1:1,25～40 ℃ of constant temperature, speed with 1.0～3.5vvm after inoculation passes into filtrated air, mixed culture fermentation 48～96h;

Wherein, described fermentation medium prepares in accordance with the following methods:

Take soy peptone 5.0g; Glucose 5.0g; Yeast soaks powder 5.0g; Potassium dihydrogen phosphate 4.0g; Mass percent is 3.08% manganese sulfate solution 1mL, and deionized water 1000ml adjusts pH to be 6.50,0.1MPa sterilizing 20 minutes, standby.

2. preparation method as claimed in claim 1, is characterized in that described animal protein comprises: the new blood of various animals, meat or internal organ;

Described phytoprotein is soybean protein, wheat gluten, zein, rapeseed protein or cottonseed protein.

3. preparation method as claimed in claim 1, is characterized in that described bacillus comprises one or more in bacillus subtilis, bacillus licheniformis, bafillus natto;

Described saccharomycete comprises one or more in saccharomyces cerevisiae, candida utili;

Described lactic acid bacteria comprises one or more in Lactobacillus casei, Lactobacillus plantarum, lactobacillus acidophilus, streptococcus thermophilus, lactobacillus bulgaricus, Lactobacillus helveticus.

4. preparation method as claimed in claim 1, it is characterized in that described protease comprise papain, bromelain, neutral proteinase, alkali protease, trypsase, protein incision enzyme, excision enzyme and food flavor enzyme wherein one or more; The described intensification enzyme that goes out is after enzymolysis finishes, and enzymolysis liquid is warming up to 85～90 ℃, constant temperature 30～60min.

5. preparation method as claimed in claim 1, it is characterized in that described concentrated be to adopt film concentrated, by following current economic benefits and social benefits falling film condenser, being concentrated into dry matter content is 15～60%, 80～85 ℃ of temperature of effect, vacuum 0.90～0.95Mpa when concentrated; Two 70～80 ℃ of effect temperature, vacuum 0.80～0.85Mpa.

6. preparation method as claimed in claim 1, it is characterized in that described dry, granulation are that concentrated after fermentation liquid is dry through 70～80 ℃ of circulating airs, or adopt the mode of vacuum spray drying that concentrate Direct spraying is dried to powder, add carrier, the mode of taking low temperature to granulate is granulated, and finished granule moisture is 8～12%.

7. the prebiotic peptide biology feed additive preparing according to the method described in claim 1-6 any one.

8. the application of prebiotic peptide biology feed additive claimed in claim 7 in preparing animal feed.