CN108925586A - A kind of antimicrobial composition preparation improving plant pest management effect - Google Patents
A kind of antimicrobial composition preparation improving plant pest management effect Download PDFInfo
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- CN108925586A CN108925586A CN201810972897.2A CN201810972897A CN108925586A CN 108925586 A CN108925586 A CN 108925586A CN 201810972897 A CN201810972897 A CN 201810972897A CN 108925586 A CN108925586 A CN 108925586A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
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- Agronomy & Crop Science (AREA)
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Abstract
The invention belongs to field of biotechnology, it is related to for preventing and treating plant pest and promoting the antimicrobial composition microbial inoculum of plant growth, this antimicrobial composition preparation is to be used for the combination preparation that the microbial bacterial agent of plant pest is developed and prevented and treated to coordinate plant growth made of ratio by sustained release microbial inoculum that instant microcapsule microbial agent and sterilization made of bacillus amyloliquefaciens or insecticidal microorganism embeds with cere material according to 1-5:1-9.
Description
Technical field
The invention belongs to field of biotechnology, it is related to for preventing and treating plant pest and promoting the microbial bacterial agent of plant growth.
Background technique
Chemical pesticide is played in terms of controlling agricultural pest, protection agricultural production security and in terms of reduction
Important function.With the development of the chemical industry with the expansion of agrochemical fertilizer use scope, the quantity and kind of chemical pesticide are all
It is being continuously increased, present World chemical pesticide total output (as active ingredients) is more than 3,000,000 tons.Due to chemical pesticide and chemical fertilizer
Long-term a large amount of applications, brought being continuously increased for serious environmental pollution and disease pest drug resistance.Many countries all put into
Substantial contribution researchs and develops the novel formulation that can substitute or reduce chemical pesticide and chemical fertilizer, to keep the sustainable development of agricultural production
Exhibition.
With the requirement of economic development and environment sustainable development, many countries be all proposed decrement using chemical pesticide and
The policies and measures of chemical fertilizer.Agricultural disease control measure that is new, meeting agricultural sustainable development is found to produce as current agricultural
The hot spot of research.Since the advantages of biological pesticide product is pollution-free, noresidue, causes the concern of people.Wherein, microbial bacterial agent
It is the main source of biological pesticide, including using Dipel as the microbial insecticide of representative, bacillus subtilis is representative
Microbial bactericide.
The fatal defects of microbial pesticide are to be easy death, shelf life is short to extraneous condition responsive under natural situation.It is existing
In many microbial pesticides in order to extend shelf life, by fermentation optimization, the strong life of the resistance such as gemma, chlamydospore is turned out
Then wettable powder, water dispersible granules etc. are made in object.This method increases microbial pesticide nature to a certain extent
Under the conditions of resistance, the especially patent of invention of microcapsule formulations (CN102870816A), to the shelf for solving microbial pesticide
Phase problem plays positive effect.
It is found in production practices, cannot usually effectively prevent target pest and disease damage using single microorganism microbial inoculum, because natural
In boundary microorganism be in the form of ecological community existing for.Therefore, more than one microorganism reasonable combinations are used, it is micro- to improving
The biological control effect of biological pesticide has positive effect.But due to the antagonism of microbial bacteria inter-species, when by more than one
When microbial pesticide is applied in combination, the effect of how avoiding antagonism between these microorganisms from influencing biological pesticide, is played pair
It is a significant challenge that biological pesticide, which is applied in combination,.
The present invention is by the way that instant microcapsule microbial agent made of bacillus amyloliquefaciens to be made with sterilization or insecticidal microorganism
The combination of sustained release microbial inoculum, solve the antagonism of beneficial microbe inter-species by the time difference, and pass through quick-acting and slow combinations effects
The effect for improving microbial bacterial agent prevention and treatment plant pest should be extended.
Summary of the invention
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or value are answered
When be interpreted as comprising close to these ranges or value value.For numberical range, between the endpoint value of each range, Ge Gefan
Can be combined with each other between the endpoint value and individual point value enclosed, and individually between point value obtain it is one or more new
Numberical range, these numberical ranges should be considered as specific open herein.
For overcome the deficiencies in the prior art, the present invention by instant microcapsule microbial agent made of bacillus amyloliquefaciens with kill
Raising microbial bacterial agent made of ratio of the sustained release microbial inoculum that bacterium or insecticidal microorganism are embedded with cere material according to 1-5:1-9 is anti-
The life assemblage preparation for insect pest effect of curing the disease, the function of combination preparation be including but not limited to used for coordinate plant growth development and
The combination preparation for preventing and treating the microbial bacterial agent of plant pest.
The sterilization microorganism of selection includes but is not limited to bacillus subtilis, Trichoderma, thick spore Verticillium dahliae, the false list of fluorescence
Born of the same parents bacterium, powdery mildew bacterial parasite are sustained at least the one of microbial bacterial agent made of bacillus pumilus and bacillus amyloliquefaciens etc.
Kind, it is more preferred to cere made of Pseudomonas fluorescens is sustained microbial bacterial agent.
The insecticidal microorganism of selection includes but is not limited to bacillus firmus, bacillus thuringiensis, short steady gemma bar
Microbial bacterial agent is sustained made of at least one of bacterium, muscardine, green muscardine fungus and bacillus amyloliquefaciens etc., it is more preferred to
Cere made of bacillus thuringiensis is sustained microbial bacterial agent.
In the present invention, the cere material include but is not limited to lac, mountain tallow, atoleine, morpholine fatty acid salt,
Pentalyn, one of palm wax and ester type waxes or a variety of, it is more preferred to lac.
In the present invention, microbial inoculum is sustained made of the sterilization or insecticidal microorganism, it is characterised in that: selected micro- life
Object includes the gemma of active microorganism cell or suspend mode or spore adsorbed with adsorbent material after be suspended in cere material emulsion,
The spray-dried bacterial content that is made reaches 108The cere of cfu/g or more is sustained microbial inoculum.
In the present invention, the adsorbent material, including but not limited to active carbon, diatomite, white carbon black, medical stone, height
Ridge soil, wheat bran, chaff shell, starch, porous-starch, soluble starch, polysaccharide, one of oligosaccharides and macroporous adsorbent or a variety of,
More preferably soluble starch.
In the present invention, the plant pest, the microorganism such as nematode for plant of including but not limited to causing harm, fungi or thin
Disease and biting mouthparts caused by bacterium etc. is infected, sucking mouth parts, rasping-sucking mouthparts, scratching mouthparts and add suction mouthpart
Insect pest caused by insect.
Present invention solves the technical problem that being that bacillus amyloliquefaciens are made to instant microcapsule microbial agent, bacterium is sustained with being made
The sterilization of agent or insecticidal microorganism combination, antagonism when different microorganisms strain combination being overcome to use are formed synergistic
Prevent and treat plant pest.Innovative point of the invention is first is that the characteristics of this combination is using bacillus amyloliquefaciens, are administered to environment
In after the water suction of instant microcapsule microbial agent sprout, decomposed by the enzyme that Bacillus amyloliquefaciens are secreted during flourish
These microbial bacterias are released from cere coating, overcome microorganism by the time difference by the cere of embedding sustained release microbial inoculum
Antagonism between strain；Innovative point is second is that enhance the energy that microbial bacterial agent resists adverse circumstance by instant and sustained release combination
Power extends the effect for improving microbial bacterial agent and preventing and treating plant pest by quick-acting and slow combined effects.
Specific embodiment
The present invention is further described according to following examples, one skilled in the art will appreciate that following embodiments to the present invention only
Only play the role of explanation.Without departing from the premise in the spirit of the present invention, exist to any improvement of the invention done and substitution
In the scope of protection of the invention.
Embodiment one
2.3 x 10 are made by the method for CN102870816A in the bacillus amyloliquefaciens of LB Liquid Culture9Cfu/g microcapsules
Agent.
Embodiment two
It is added to after the Pseudomonas fluorescens of liquid fermentation and culture is adsorbed with soluble starch in 20% shellac solution and sufficiently stirs
It mixes uniformly, 3.2 x 10 are made in spray drying9The Pseudomonas fluorescens of cfu/g cere embedding is sustained microbial inoculum.
Embodiment three
It is added to after the short steady bacillus of liquid fermentation and culture is adsorbed with soluble starch in 20% shellac solution and sufficiently stirs
It mixes uniformly, 1.1 x 10 are made in spray drying9The short steady bacillus of cfu/g cere embedding is sustained microbial inoculum.
Embodiment four
2,500 mL beaker is taken, the cere embedding of the preparation of 5g embodiment one is directly added into after addition 100 mL of aqua sterilisa
Pseudomonas fluorescens be sustained microbial inoculum.2.3 x of bacillus amyloliquefaciens is added in another beaker in 100 mL aqua sterilisas
1085 g of cfu/g microcapsule formulations adds the glimmering of the cere embedding of the preparation of 5g embodiment one after 28 DEG C of shaking table cultures for 24 hours r
Light pseudomonad is sustained microbial inoculum, shaking table culture 10min, 30min, 60min, 12 hr, 24 hr.Then it carries out a series of dilute
LB plate is released and applied, is put into after cultivating 48h in 28 DEG C of incubators, counts to survey and releases bacterium number amount (table one).As a result as can be seen that cere
Coating it is longer can to release the bacterium time with the protection Pseudomonas fluorescens of long period, and bacillus amyloliquefaciens are added can be significant
That accelerates cere sustained release microbial inoculum releases bacterium speed.
One bacillus amyloliquefaciens of table release sustained release Pseudomonas fluorescens the influence of bacterium speed
Embodiment five
By 750 grams of Pseudomonas prepared with embodiment two of bacillus amyloliquefaciens microcapsule formulations prepared by embodiment one
Bacterium is sustained 250 grams of microbial inoculum and is uniformly mixed, and sterilization antimicrobial composition preparation is made.
Embodiment six
By 300 grams of short steady gemma bars prepared with embodiment three of bacillus amyloliquefaciens microcapsule formulations prepared by embodiment one
Bacterium is sustained 700 grams of microbial inoculum and is uniformly mixed, and insecticidal microorganism combination preparation is made.
Embodiment seven
The sterilization antimicrobial composition preparation for taking embodiment five to prepare, by dilution even spraying to having occurred and that angular leaf spot of cucumber
Cucumber seedling be tested, antimicrobial composition preparation is divided into three concentration dilutions as processing, and every 50 plants of processing is repeated 4 times.Examination
It tests and is set as 5 processing: processing 1: 300 times of medical fluids of antimicrobial composition preparation diluent altogether；Processing 2: antimicrobial composition preparation diluent
500 times of medical fluids；Processing 3: 750 times of medical fluids of antimicrobial composition preparation diluent；Handle 4:2.3 x 109Cfu/g Pseudomonas fluorescens
Microcapsulated granule dilutes 300 times of medical fluids；Processing 5: clear water control.It is primary to be spaced 5 days sprays, uses Investigate incidence after 2 times.As a result
Such as table, adjoining tree morbidity is serious, and Pseudomonas fluorescens microcapsulated granule early period can be with controlling disease, but the lasting period is short, microorganism
Three concentration of combination preparation can effectively control disease, and the lasting period significantly extends, and the results are shown in Table two.
The effect of two antimicrobial composition preparation of table prevention and treatment angular leaf spot of cucumber
1After data with the different expressions processing of letter between there were significant differences.
Claims (7)
1. a kind of antimicrobial composition preparation for improving plant pest management effect, which is characterized in that be related to that starch gemma will be solved
The sustained release microbial inoculum that instant microcapsule microbial agent made of bacillus and sterilization or insecticidal microorganism are embedded with cere material is according to 1-5:1-9
Ratio made of improve microbial bacterial agent prevention and treatment pest and disease damage effect antimicrobial composition preparation, the function of combination preparation include but
It is not limited to that the combination preparation of the microbial bacterial agent of plant pest is developed and prevented and treated for coordinate plant growth.
2. antimicrobial composition preparation according to claim 1, it is characterised in that: the sterilization of sustained release microbial inoculum made of described is micro-
Biology including but not limited to bacillus subtilis, Trichoderma, thick spore Verticillium dahliae, Pseudomonas fluorescens, powdery mildew bacterial parasite is short
At least one of microbial bacterial agent is sustained made of bacillus pumilus and bacillus amyloliquefaciens etc., it is more preferred to which fluorescence is false
Cere made of monad is sustained microbial bacterial agent.
3. antimicrobial composition preparation according to claim 1, it is characterised in that: the desinsection of sustained release microbial inoculum made of described is micro-
Biology includes but is not limited to bacillus firmus, bacillus thuringiensis, short steady bacillus, muscardine, green muscardine fungus reconciliation
Microbial bacterial agent is sustained made of at least one of bacillus amyloliquefaciens etc., it is more preferred to made of bacillus thuringiensis
Cere is sustained microbial bacterial agent.
4. cere material made of sterilization according to claim 1 or insecticidal microorganism, it is characterised in that: the cere material
Material includes but is not limited to lac, mountain tallow, atoleine, morpholine fatty acid salt, pentalyn, in palm wax and ester type waxes
It is one or more, it is more preferred to lac.
5. sterilization according to claim 1 or insecticidal microorganism are sustained microbial inoculum, it is characterised in that: selected microorganism packet
The gemma or spore for including active microorganism cell or suspend mode are suspended in cere material emulsion after being adsorbed with adsorbent material, through spraying
Mist drying is made bacterial content and reaches 108The cere of cfu/g or more is sustained microbial inoculum.
6. plant pest according to claim 1, the microorganism such as nematode for plant of including but not limited to causing harm, fungi or
Disease and biting mouthparts caused by bacterium etc. is infected, sucking mouth parts, rasping-sucking mouthparts, scratching mouthparts and add suction mouthpart
Insect caused by insect pest.
7. adsorbent material according to claim 5, including but not limited to active carbon, diatomite, white carbon black, medical stone are high
Ridge soil, wheat bran, chaff shell, starch, porous-starch, soluble starch, polysaccharide, one of oligosaccharides and macroporous adsorbent or a variety of,
More preferably soluble starch.
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| CN201810972897.2A CN108925586A (en) | 2018-08-24 | 2018-08-24 | A kind of antimicrobial composition preparation improving plant pest management effect |
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| CN201810972897.2A CN108925586A (en) | 2018-08-24 | 2018-08-24 | A kind of antimicrobial composition preparation improving plant pest management effect |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109845514A (en) * | 2018-12-26 | 2019-06-07 | 云南省农业科学院农业环境资源研究所 | Banana Fusarium Wilt Prevention and Control Method Based on Encapsulated Beneficial Microorganisms in Simulated Rhizosphere Soil |
| CN110172003A (en) * | 2019-06-06 | 2019-08-27 | 哈尔滨坤丰生物工程有限公司 | A kind of preparation of biological bacteria capsule and method of administration |
| CN110358709A (en) * | 2019-07-23 | 2019-10-22 | 华东理工大学 | A kind of Pseudomonas fluorescens microcapsule formulations and its preparation method and application |
| CN111979220A (en) * | 2019-05-24 | 2020-11-24 | 成都特普生物科技股份有限公司 | Carrier molecular chaperone for activating microbial preparation |
| CN113088461A (en) * | 2019-12-23 | 2021-07-09 | 中国科学院天津工业生物技术研究所 | Microbial compound inoculant and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109845514A (en) * | 2018-12-26 | 2019-06-07 | 云南省农业科学院农业环境资源研究所 | Banana Fusarium Wilt Prevention and Control Method Based on Encapsulated Beneficial Microorganisms in Simulated Rhizosphere Soil |
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| CN110358709A (en) * | 2019-07-23 | 2019-10-22 | 华东理工大学 | A kind of Pseudomonas fluorescens microcapsule formulations and its preparation method and application |
| CN113088461A (en) * | 2019-12-23 | 2021-07-09 | 中国科学院天津工业生物技术研究所 | Microbial compound inoculant and application thereof |
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Application publication date: 20181204 |