CN109845514A - Banana Fusarium Wilt Prevention and Control Method Based on Encapsulated Beneficial Microorganisms in Simulated Rhizosphere Soil - Google Patents
Banana Fusarium Wilt Prevention and Control Method Based on Encapsulated Beneficial Microorganisms in Simulated Rhizosphere Soil Download PDFInfo
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- 241000234295 Musa Species 0.000 title claims abstract description 64
- 235000018290 Musa x paradisiaca Nutrition 0.000 title claims abstract description 62
- 230000009286 beneficial effect Effects 0.000 title claims abstract description 60
- 244000005700 microbiome Species 0.000 title claims abstract description 43
- 239000002689 soil Substances 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 20
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 title claims abstract description 6
- 241000223218 Fusarium Species 0.000 title claims abstract description 6
- 230000002265 prevention Effects 0.000 title claims abstract 4
- 239000002245 particle Substances 0.000 claims abstract description 16
- 239000008223 sterile water Substances 0.000 claims abstract description 14
- 238000012360 testing method Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 6
- 241000194103 Bacillus pumilus Species 0.000 claims abstract description 6
- 241000589540 Pseudomonas fluorescens Species 0.000 claims abstract description 6
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 6
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 5
- 238000009395 breeding Methods 0.000 claims description 4
- 230000001488 breeding effect Effects 0.000 claims description 4
- 230000008635 plant growth Effects 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 22
- 201000010099 disease Diseases 0.000 description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 15
- 238000004088 simulation Methods 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 9
- 238000011081 inoculation Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 230000005540 biological transmission Effects 0.000 description 4
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 210000002421 cell wall Anatomy 0.000 description 3
- 210000000416 exudates and transudate Anatomy 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
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- 235000021015 bananas Nutrition 0.000 description 2
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- 239000000203 mixture Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000879841 Fusarium oxysporum f. cubense Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 240000000905 Nymphoides indica Species 0.000 description 1
- 235000017590 Nymphoides indica Nutrition 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 208000026487 Triploidy Diseases 0.000 description 1
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- 210000001723 extracellular space Anatomy 0.000 description 1
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- 235000008434 ginseng Nutrition 0.000 description 1
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Abstract
本发明提供一种基于模拟根际土壤包埋有益微生物的香蕉枯萎病防控方法。方法包括：在香蕉苗育苗期，向香蕉苗的根际接种有益微生物，有益微生物包埋于模拟根际土壤SRS颗粒中，有益微生物的SRS颗粒放置在试管苗根部下方1厘米处。所述有益微生物包括：荧光假单胞菌、哈茨木霉、恶臭芽孢杆菌、短小芽孢杆菌、解淀粉芽孢杆菌。若香蕉苗为组织培养试管苗，则先用无菌水释放SRS颗粒包埋的有益微生物，然后再将有益微生物直接加入生根的组培苗根部，以便有益微生物在根部定植。在香蕉苗根际接种上述五种有益微生物后，显著地改变了香蕉苗根际微生物的种群和丰度，能够有效降低枯萎病的发病程度和发病率，进而促进植株生长。
The invention provides a banana fusarium wilt prevention and control method based on simulating rhizosphere soil embedding beneficial microorganisms. The method includes: inoculating beneficial microorganisms into the rhizosphere of banana seedlings during the seedling-raising period of banana seedlings, the beneficial microorganisms are embedded in SRS particles of simulated rhizosphere soil, and the SRS particles of beneficial microorganisms are placed 1 cm below the roots of test-tube seedlings. The beneficial microorganisms include: Pseudomonas fluorescens, Trichoderma harzianum, Bacillus putida, Bacillus pumilus, and Bacillus amyloliquefaciens. If the banana seedlings are tissue culture test tube seedlings, the beneficial microorganisms embedded in the SRS particles are firstly released with sterile water, and then the beneficial microorganisms are directly added to the roots of the rooted tissue culture seedlings, so that the beneficial microorganisms can colonize the roots. After inoculating the above five beneficial microorganisms in the rhizosphere of banana seedlings, the population and abundance of microorganisms in the rhizosphere of banana seedlings were significantly changed, which could effectively reduce the incidence and incidence of Fusarium wilt, thereby promoting plant growth.
Description
Technical field
The application belongs to banana disease prevention techniques field, specifically, being related to a kind of based on simulation rhizosphere soil embedding
Beneficial microorganism banana blight preventing control method.
Background technique
Banana blight bacteria by Fusarium oxysporum Cuba specialized form (Fusarium oxysporum f.sp.cubense,
Foc) infecting causes, and is a kind of typical tracheomycosis evil.Foc invades vascular bundle along root system wound, and constantly prolongs upwards
It stretches, causes vascular bundle downright bad, last entire plant is withered and dead.
Currently, banana blight has spread to each main banana production state in the whole world, become most destructive banana disease
One of.Banana blight bacteria can be propagated by seedling, means of transport and personnel's dealing in spite of illness.Banana blight passes
The preventing control method of system mainly has using chemical pesticide and plantation disease-resistant variety.Chemical pesticide is not only ineffective, can also pollute ring
Border and influence farming operations personnel are physically and mentally healthy；Disease-resistant variety mostly can only the single biological strain of resisting banana vascular wilt, several
There are the danger of large area outburst when biological strain is invaded simultaneously.
Summary of the invention
In view of the above drawbacks of the prior art, the application provides a kind of based on simulation rhizosphere soil embedding beneficial microorganism
Banana blight preventing control method.
It is disclosed by the invention that beneficial microorganism banana blight preventing control method is embedded based on simulation rhizosphere soil, comprising:
In banana seedlings nursery stage, it is inoculated with beneficial microbe to the rhizosphere of banana seedlings, beneficial microbe is embedded in simulation rhizosphere
In soil SRS particle, beneficial microorganism SRS particle is placed on 1 centimeters below test tube seedling root；The beneficial microbe packet
It includes: Pseudomonas fluorescens, Trichoderma harzianum, stench bacillus, bacillus pumilus, bacillus amyloliquefaciens.
It is as described above that beneficial microorganism banana blight preventing control method is embedded based on simulation rhizosphere soil, further includes:
If banana seedlings are tissue cultures test tube seedling, the beneficial microbe of SRS particle embedding is first discharged with sterile water, then
Beneficial microbe is directly added into the tissue-cultured seedling root taken root again, so that beneficial microbe is colonized in root.
It is as described above that beneficial microorganism banana blight preventing control method is embedded based on simulation rhizosphere soil, it is preferable that
The kind of the banana seedlings is cloud any of several broadleaf plants No. 1 of autonomous breeding.
It is provided by the invention based on simulation rhizosphere soil embedding beneficial microorganism banana blight preventing control method have with
Lower advantage:
Pseudomonas fluorescens, Trichoderma harzianum, stench bacillus, bacillus pumilus, Xie Dian are inoculated in banana seedlings rhizosphere
After afnyloliquefaciens, population and the abundance of banana seedlings rhizosphere microorganism are changed significantly, can be effectively reduced the hair of wilt disease
Course of disease degree and disease incidence, and then promote plant strain growth.
Detailed description of the invention
Fig. 1 is that the rhizosphere of banana seedlings is inoculated with the quantity variation schematic diagram of banana seedlings root endophyte after beneficial microbe；
Fig. 2 is the plant root schematic diagram that test tube seedling is inoculated with that sterile water discharges SRS beneficial microbe 40m l processing；
Fig. 3 is the plant root schematic diagram that test tube seedling is inoculated with that sterile water discharges SRS beneficial microbe 20m l processing；
Fig. 4 is that test tube seedling is not inoculated with beneficial microorganism plant root schematic diagram.
Specific embodiment
Presently filed embodiment is described in detail below in conjunction with embodiment, whereby to the application how application technology hand
Section solves technical problem and reaches the realization process of technical effect to fully understand and implement.
Banana blight bacteria is a kind of typical tracheomycosis evil and a kind of disease of soil-borne, germ
Thick plateau spore can survive many decades in the soil.Due to the feature of banana itself triploid and breeding for disease resistance period length, in short term
Interior control disease is not easy.By the antagonism machine for studying banana rhizosphere microorganism and root system endophyte and Pathogen of Fusarium Wilt
System, excavation, identification and screening beneficial rhizosphere microorganism associated with disease-resistant material and root system endophyte COMMUNITY CHARACTERISTICS (type,
Abundance, structure) and correlation function gene, the present invention, which will be given by, improves banana plant in banana seedlings rhizosphere addition beneficial microbe
Method of the strain to the resistance of wilt disease.
It is disclosed by the embodiments of the present invention that beneficial microorganism banana blight preventing control method is embedded based on simulation rhizosphere soil,
Include:
In banana seedlings nursery stage, it is inoculated with beneficial microbe to the rhizosphere of banana seedlings, beneficial microbe is embedded in simulation rhizosphere
In soil SRS particle, beneficial microorganism SRS particle is placed on 1 centimeters below test tube seedling root；The beneficial microbe packet
It includes: Pseudomonas fluorescens, Trichoderma harzianum, stench bacillus, bacillus pumilus, bacillus amyloliquefaciens.
Fig. 1 is that the rhizosphere of banana seedlings is inoculated with the quantity variation schematic diagram of banana seedlings root endophyte after beneficial microbe.Ginseng
It examines shown in Fig. 1, beneficial microbe is inoculated in the rhizosphere of banana seedlings, specifically, inoculation is embedded in simulation rhizosphere through sterile water release
The beneficial microorganism amount of beneficial microbe in soil SRS particle, sterile water release distinguishes 40ml and 20ml, fragrant after 12 days
The quantity variation of any of several broadleaf plants seedling root endophyte is as shown in Figure 1.
Rhizosphere is complicated plant-soil-microbial interaction place, it refer to plant root table and by
The soil region that root system directly affects is to be different from the area part Wei Yutu of the soil body in nature in physics, chemistry and biology.
In a particular application, if banana seedlings are tissue cultures test tube seedling, first using simulation rhizosphere soil SRS particle embedding
Then embedding beneficial microorganism SRS particle is placed on 1 centimeters below test tube seedling root again by beneficial microbe.
The present invention implements the embedding used, (simulated rhizosphere soil simulates rhizosphere to covering material SRS
Soil) it is a kind of inert, freeze-drying nano material.
It is as described above that beneficial microorganism banana blight preventing control method is embedded based on simulation rhizosphere soil, it is preferable that
The kind of the banana seedlings is cloud any of several broadleaf plants No. 1.
Different banana variety root exudates have differences, and the difference of root exudates will affect the population of rhizosphere microorganism
Structure and abundance eventually lead to the change of rhizosphere microbial group.Soil is sought in addition, rhizosphere microorganism group will affect root system
Feeding utilization efficiency.The composition and relative amount of root exudates also will affect surrounding soil environment and to rhizosphere microorganisms
Group has an impact.The composition and functional characteristic of same rhizosphere microorganism will affect the growth and development and its adaptability of plant.Cause
This, rhizosphere microorganism diversity will finally influence soil ecosystem.
Given below is the test example of technical solution provided by the invention:
One, banana seedlings are No. 1 tissue culturing seedling of disease-resistant variety cloud any of several broadleaf plants of autonomous breeding.
Two, using the SRS particle embedding Pseudomonas fluorescens of Nutriss company, Britain offer, Trichoderma harzianum, stench gemma
Bacillus, bacillus pumilus, bacillus amyloliquefaciens.First simulation rhizosphere soil (Simulated is discharged with sterile water
Rhizosphere Soil, SRS) particle embedding beneficial microbe, then beneficial microbe is directly added into the group taken root again
Seedling root is trained, so that beneficial microbe is colonized in root.
Three, rhizosphere after two weeks, is simulated by transmission electron microscope observation and embeds five kinds of beneficial microbe endophytes to cloud
Growing state of the effect of inoculation and endophyte that any of several broadleaf plants is No. 1 in tissue culture seedlings of bananas, observation discovery:
First group: test tube seedling is inoculated with the plant of sterile water release SRS beneficial microbe 40ml processing；First group of plant root
The quantity of endophyte be averaged each observation sample be 7 endophytes (referring to fig. 1 and fig. 2), and compare be not observed in
Raw bacterium (with reference to shown in Fig. 4).In Fig. 2, at No. 1 inoculation sterile water release SRS beneficial microbe 40ml of transmission electron microscope observing cloud any of several broadleaf plants
The banana endophyte quantity B=endogenetic bacteria of reason, CW=cell wall, GA=golgiosome.
Second group: test tube seedling is inoculated with the plant of sterile water release SRS beneficial microbe 20ml processing；Second group of plant root
The quantity of endophyte each observation sample that is averaged is 11 endophytes (with reference to shown in Fig. 1 and Fig. 3), and in compareing and being not observed
Raw bacterium (with reference to shown in Fig. 4).In Fig. 3, at No. 1 inoculation sterile water release SRS beneficial microbe 20ml of transmission electron microscope observing cloud any of several broadleaf plants
The banana endophyte quantity B=endogenetic bacteria of reason, CW=cell wall.In Fig. 4, No. 1 inoculation sterile water of transmission electron microscope observing cloud any of several broadleaf plants
As the banana endophyte situation N=nucleus of control treatment, CW=cell wall, IS=intercellular spaces, control treatment do not have
Observe endophyte.
After handling tissue culture seedlings of bananas two weeks by endophyte, banana seedlings are transplanted to greenhouse hardening, show robust growth.?
After banana seedlings are grown 6 weeks, banana blight bacteria spore suspension is inoculated in PDA culture medium, 25-30 DEG C of dark surrounds is placed in
Sterile water is added in middle culture 7d, and being modulated into spore concentration with blood counting chamber is 106The spore suspension of cfu/ml is to banana seedlings
Leaching root 30min inoculation is carried out, is then transplanted in basin alms bowl.The number of blade, false stem perimeter and the wilt disease of Banana Growth are carried out simultaneously
Disease incidence and disease index carry out follow-up investigation, and 2 referring to shown in table 1 and table, plant is significantly promoted after handling by SRS
Grow and improve the resistance to banana blight TR4.
1 cloud any of several broadleaf plants of the table No. 1 growth performance situation table for handling and being inoculated with through endophyte after banana blight bacteria
Plant and false stem disease index (%) table of the table 2 after inoculation TR4 eight weeks
Above description shows and describes several preferred embodiments of the present application, but as previously described, it should be understood that the application
Be not limited to forms disclosed herein, should not be regarded as an exclusion of other examples, and can be used for various other combinations,
Modification, and can be in the application contemplated scope, modifications can be made through the above teachings or related fields of technology or knowledge.And this
The modifications and changes that field personnel are carried out do not depart from spirit and scope, then all should be in the application appended claims
Protection scope in.
Claims (3)
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811598693.3A CN109845514A (en) | 2018-12-26 | 2018-12-26 | Banana Fusarium Wilt Prevention and Control Method Based on Encapsulated Beneficial Microorganisms in Simulated Rhizosphere Soil |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111436349A (en) * | 2020-03-13 | 2020-07-24 | 河海大学 | Seedling raising substrate for cultivating salt-resistant plant seedlings and preparation method and application thereof |
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| CN105660254A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method and biocontrol agent for banana vasicular wilt |
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| CN111436349A (en) * | 2020-03-13 | 2020-07-24 | 河海大学 | Seedling raising substrate for cultivating salt-resistant plant seedlings and preparation method and application thereof |
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Application publication date: 20190607 |