CN105053015A - Biopesticide for controlling cercospora varrireolorWinter, and preparation method for biopesticide - Google Patents
Biopesticide for controlling cercospora varrireolorWinter, and preparation method for biopesticide Download PDFInfo
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- 241001157813 Cercospora Species 0.000 title 1
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Abstract
本发明属于生物技术领域，具体涉及一种用于防治牡丹褐斑病的生物农药及其制备方法。本发明采用对牡丹褐斑病病原真菌牡丹枝孢霉有拮抗作用的枯草芽孢杆菌HS5B5与生物肥和波尔多液复配，能够更加有效的抑制牡丹枝孢霉的生长繁殖，进而达到防治牡丹褐斑病的目的，同时波尔多液对牡丹其它真菌病害也有抑制作用，通过与枯草芽孢杆菌和生物肥的复配后，增强了对牡丹真菌病害的防治效果。本发明在枯草芽孢杆菌HS5B5中加入生物肥和波尔多液，还能够延长花期，与常规种植相比平均延长2-3天。本发明采用生物农药防治的方法，对人畜安全，对环境无污染，高效广谱，不易产生抗药性。The invention belongs to the field of biotechnology, and in particular relates to a biological pesticide for preventing and treating peony brown spot and a preparation method thereof. The present invention adopts Bacillus subtilis HS5B5, which has antagonistic effect on the pathogenic fungus Cladosporium peony, to compound with biological fertilizer and Bordeaux mixture, which can more effectively inhibit the growth and reproduction of Cladosporium peony, and further achieve the prevention and treatment of brown spot of peony At the same time, Bordeaux mixture also has inhibitory effect on other fungal diseases of tree peony. After compounding with Bacillus subtilis and biological fertilizer, the control effect on fungal diseases of tree peony is enhanced. In the present invention, adding biological fertilizer and Bordeaux mixture to the bacillus subtilis HS5B5 can also prolong the flowering period, which is 2-3 days on average compared with conventional planting. The invention adopts the biological pesticide control method, which is safe for humans and animals, has no pollution to the environment, has high efficiency and broad spectrum, and is not easy to produce drug resistance.
Description
技术领域 technical field
本发明属于生物技术领域，具体涉及一种用于防治牡丹褐斑病的生物农药及其制备方法。 The invention belongs to the field of biotechnology, and in particular relates to a biological pesticide for preventing and treating peony brown spot and a preparation method thereof.
背景技术 Background technique
牡丹是我国著名的观赏花卉，在生长发育和越冬过程中，容易受到病原微生物的浸染而导致病变。牡丹病害发生种类多，危害严重，这些病害大多数是真菌性病害，比如褐斑病、灰霉病、锈病、白粉病和紫纹羽病等。它们能够引起牡丹叶局部坏死或整叶枯死，危害牡丹的叶、枝条和根茎部，使牡丹提前落叶，影响其营养生长和观赏价值。牡丹褐斑病是最为严重的病害之一，主要危害叶片，也可侵染茎和花。病害多在开花前后发生，一直延续到枯叶期。病原真菌为牡丹枝孢霉（Cladosporiumpaeoniae），属半知菌亚门，丝孢菌纲，丛梗孢目，枝孢菌属。病原以菌丝体或分生孢子在枯叶或土壤里越冬，借助风雨传播夏初开始发生，秋季危害严重，高温、高湿、光照不足、通风不良、连作等均有利于病害发生。 Peony is a famous ornamental flower in my country. During the process of growth and overwintering, it is easily infected by pathogenic microorganisms and causes disease. There are many types of peony diseases, and the damage is serious. Most of these diseases are fungal diseases, such as brown spot, gray mold, rust, powdery mildew and purple feather disease. They can cause partial necrosis of peony leaves or the death of whole leaves, harm the leaves, branches and rhizomes of peony, make peony fall leaves in advance, and affect its vegetative growth and ornamental value. Peony brown spot is one of the most serious diseases, which mainly damages the leaves, but can also infect stems and flowers. The disease mostly occurs before and after flowering and continues until the dead leaf stage. The pathogenic fungus is Cladosporium paeoniae , which belongs to the subphylum Deuteromycetes, the class Hyphomycetes, the order Cladosporium, and the genus Cladosporium. The pathogen survives the winter in dead leaves or soil with mycelium or conidia, and spreads by wind and rain. It begins to occur in early summer, and the damage is serious in autumn. High temperature, high humidity, insufficient light, poor ventilation, and continuous cropping are all conducive to the occurrence of the disease.
牡丹褐斑病的防治现在主要依靠化学药物的使用，这不仅对人体健康和生态环境安全有害，而且容易引起病原微生物抗药性。生物农药是利用微生物或其代谢产物控制农作物病虫害或调节农作物生长的防治方法，但其在牡丹真菌病害防治上的研究和应用还很少。为了增强牡丹产业的快速发展，研制出对人体健康和生态环境安全无害、不易引起病原抗药性的生物农药迫在眉睫，枯草芽孢杆菌HS5B5通过生物拮抗作用对牡丹枝孢霉为病原真菌的牡丹褐斑病有明显的抑制效果，对其他真菌病害如牡丹白粉病也有一定抑制效果但效果不明显，而生物肥和波尔多液通过微生物营养竞争和化学作用在对牡丹白粉病的抑制和植物促生、延长花期上有明显效果。 The prevention and treatment of peony brown spot disease mainly depends on the use of chemical drugs, which is not only harmful to human health and ecological environment safety, but also easily leads to drug resistance of pathogenic microorganisms. Biopesticide is a control method that uses microorganisms or their metabolites to control crop diseases and insect pests or regulate crop growth, but its research and application in the control of peony fungal diseases is still very little. In order to enhance the rapid development of the tree peony industry, it is imminent to develop biopesticides that are harmless to human health and the ecological environment and are not likely to cause pathogenic drug resistance. It has an obvious inhibitory effect on other fungal diseases such as peony powdery mildew, but the effect is not obvious. However, biological fertilizer and Bordeaux mixture play a role in inhibiting tree peony powdery mildew and promoting plant growth and prolonging plant growth through microbial nutrient competition and chemical action. There is a noticeable effect during the flowering period.
发明内容 Contents of the invention
本发明的目的是提供一种用于防治牡丹褐斑病的生物农药及其制备方法，不仅能够有效的防治牡丹褐斑病，对环境无害，而且能够有效的促进牡丹的生长发育，延长花期，防治其它一些常见真菌病害。 The object of the present invention is to provide a biological pesticide for preventing and treating tree peony brown spot and its preparation method, which can not only effectively prevent and treat tree peony brown spot, is harmless to the environment, but also can effectively promote the growth and development of tree peony and prolong the flowering period , Control some other common fungal diseases.
本发明所采取的技术方案是：一种用于防治牡丹褐斑病的生物农药，所述生物农药由枯草芽孢杆菌、生物肥和波尔多液制成，且制备过程所用的各组分重量份数分别为：枯草芽孢杆菌1-2份，生物肥8-10份和波尔多液4-5份，所述枯草芽孢杆菌命名为枯草芽孢杆菌（Bacillussubtilis）HS5B5，该菌株保藏于中国微生物菌种保藏管理委员会普通微生物中心，地址：北京市朝阳区北辰西路1号院3号中国科学院微生物研究所，保藏号为：CGMCCNo.6088。 The technical scheme adopted by the present invention is: a kind of biopesticide used for preventing and treating peony brown spot, said biopesticide is made of Bacillus subtilis, biological fertilizer and Bordeaux mixture, and the parts by weight of each component used in the preparation process They are: 1-2 parts of Bacillus subtilis, 8-10 parts of biological fertilizer and 4-5 parts of Bordeaux mixture. The Bacillus subtilis is named as Bacillus subtilis ( Bacillus subtilis) HS5B5. General Microbiology Center of the Committee, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is: CGMCCNo.6088.
所述的生物农药的制备方法，包括以下步骤： The preparation method of described biopesticide, comprises the following steps:
步骤一、所述生物肥的制备 Step 1, the preparation of the biological fertilizer
在牲畜粪便中加入酵母菌，且牲畜粪便与酵母菌质量的比例为500:1，搅拌均匀后放入发酵室内发酵，6-7天后取出，制得混合物，向混合物中加入菜籽饼，且混合物与菜籽饼的质量比例为2:1，搅拌均匀后晾晒，制得生物肥； Adding yeast to livestock manure, and the ratio of livestock manure to yeast mass is 500:1, stirring evenly, putting it into a fermentation room for fermentation, taking it out after 6-7 days to prepare a mixture, adding rapeseed cake to the mixture, and The mass ratio of the mixture to the rapeseed cake is 2:1, and after stirring evenly, it is aired to obtain a biological fertilizer;
步骤二、所述波尔多液的制备 Step 2, the preparation of the Bordeaux mixture
将硫酸铜放入水中稀释，且硫酸铜与水的质量比例为1:80，搅拌均匀后，制得稀释硫酸铜，将生石灰放入水中，且生石灰和水的质量比例为1:40，搅拌均匀后，制得石灰乳，将稀释硫酸铜倒入石灰乳中，边倒边搅拌，制得波尔多液； Put copper sulfate into water to dilute, and the mass ratio of copper sulfate to water is 1:80, after stirring evenly, make diluted copper sulfate, put quicklime into water, and the mass ratio of quicklime to water is 1:40, stir After uniformity, the lime milk is obtained, and the diluted copper sulfate is poured into the lime milk, and stirred while pouring to obtain the Bordeaux mixture;
步骤三、将所述的枯草芽孢杆菌HS5B5、生物肥和波尔多液混合后搅拌均匀即制得生物农药，且生物农药中各成分的重量份数为枯草芽孢杆菌HS5B5的重量份数为1-2份，生物肥的重量份数为8-10份，波尔多液的重量份数为4-5份。 Step 3, mixing the Bacillus subtilis HS5B5, biological fertilizer and Bordeaux mixture and stirring evenly to obtain a biopesticide, and the parts by weight of each component in the biopesticide are 1-2 parts by weight of the Bacillus subtilis HS5B5 The parts by weight of biological fertilizer are 8-10 parts, and the parts by weight of Bordeaux mixture are 4-5 parts.
本发明通过使用自配的波尔多液，对生物农药中的枯草芽孢杆菌HS5B5的芽孢存在极低的抑制作用，存活率为92-95%。 By using the self-prepared Bordeaux mixture, the present invention has extremely low inhibitory effect on the spores of Bacillus subtilis HS5B5 in biological pesticides, and the survival rate is 92-95%.
本发明中的枯草芽孢杆菌（Bacillussubtilis）HS5B5，2012年5月9日保藏于中国微生物菌种保藏管理委员会普通微生物中心（CGMCC），地址：北京市朝阳区北辰西路1号院3号中国科学院微生物研究所，保藏编号：CGMCCNo.6088。本发明中的酵母菌（Trichosporoncapitatum）购买于陕西源一生物科技有限公司，该菌种保藏于中国典型培养物保藏中心，保藏菌号为CCTCCAY93140。本发明实验过程中的牡丹枝孢霉（Cladosporiumpaeoniae）购自中国农业微生物菌种保藏管理中心，菌株保藏号为ACCC36063。 The Bacillus subtilis ( Bacillus subtilis) HS5B5 in the present invention was preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC) on May 9, 2012, address: Chinese Academy of Sciences, No. 1, Courtyard 1, Beichen West Road, Chaoyang District, Beijing Institute of Microbiology, deposit number: CGMCCNo.6088. The yeast ( Trichosporoncapitatum ) in the present invention was purchased from Shaanxi Yuanyi Biotechnology Co., Ltd., and the strain was preserved in the China Center for Type Culture Collection with the preservation number CCTCCAY93140. The Cladosporium paeoniae used in the experiment of the present invention was purchased from China Agricultural Microorganism Culture Collection Management Center, and the strain preservation number is ACCC36063.
有益效果 Beneficial effect
本发明采用对牡丹褐斑病病原真菌牡丹枝孢霉的枯草芽孢杆菌HS5B5与生物肥和波尔多液复配，能够更加有效的抑制牡丹枝孢霉的生长繁殖，进而达到防治牡丹褐斑病的目的，同时波尔多液对牡丹白粉病有抑制作用，通过与枯草芽孢杆菌和生物肥的复配后，增强了对牡丹白粉病的防治效果。本发明在枯草芽孢杆菌HS5B5中加入生物肥和波尔多液，还能够延长花期，与常规种植相比平均延长2-3天。本发明采用生物农药防治的方法，对人畜安全，对环境无污染，高效广谱，不易产生抗药性。 The present invention adopts Bacillus subtilis HS5B5, which is the pathogenic fungus of Cladosporium peony, to be compounded with biological fertilizer and Bordeaux mixture, which can more effectively inhibit the growth and reproduction of Cladosporium peony, and then achieve the purpose of preventing and treating brown spot of peony At the same time, Bordeaux mixture has inhibitory effect on tree peony powdery mildew, and after compounding with Bacillus subtilis and biological fertilizer, the control effect on tree peony powdery mildew is enhanced. In the present invention, adding biological fertilizer and Bordeaux mixture to the bacillus subtilis HS5B5 can also prolong the flowering period, which is 2-3 days on average compared with conventional planting. The invention adopts the biological pesticide control method, which is safe for humans and animals, has no pollution to the environment, has high efficiency and broad spectrum, and is not easy to produce drug resistance.
具体实施方式 Detailed ways
一种用于防治牡丹褐斑病的生物农药，所述生物农药由枯草芽孢杆菌、生物肥和波尔多液制成，且制备过程所用的各组分重量份数分别为：枯草芽孢杆菌1-2份，生物肥8-10份和波尔多液4-5份，所述枯草芽孢杆菌为枯草芽孢杆菌HS5B5。 A biological pesticide for preventing and treating brown spot of peony, said biological pesticide is made of Bacillus subtilis, biological fertilizer and Bordeaux mixture, and the parts by weight of the components used in the preparation process are respectively: Bacillus subtilis 1-2 parts, 8-10 parts of biological fertilizer and 4-5 parts of Bordeaux mixture, and the bacillus subtilis is bacillus subtilis HS5B5.
所述的生物农药的制备方法，包括以下步骤： The preparation method of described biopesticide, comprises the following steps:
步骤一、所述生物肥的制备 Step 1, the preparation of the biological fertilizer
在牲畜粪便中加入酵母菌，且牲畜粪便与酵母菌质量的比例为500:1，搅拌均匀后放入发酵室内发酵，6-7天后取出，制得混合物，向混合物中加入菜籽饼，且混合物与菜籽饼的质量比例为2:1，搅拌均匀后晾晒，制得生物肥； Adding yeast to livestock manure, and the ratio of livestock manure to yeast mass is 500:1, stirring evenly, putting it into a fermentation room for fermentation, taking it out after 6-7 days to prepare a mixture, adding rapeseed cake to the mixture, and The mass ratio of the mixture to the rapeseed cake is 2:1, and after stirring evenly, it is aired to obtain a biological fertilizer;
步骤二、所述波尔多液的制备 Step 2, the preparation of the Bordeaux mixture
将硫酸铜放入水中稀释，且硫酸铜与水的质量比例为1:80，搅拌均匀后，制得稀释硫酸铜，将生石灰放入水中，且生石灰和水的质量比例为1:40，搅拌均匀后，制得石灰乳，将稀释硫酸铜倒入石灰乳中，边倒边搅拌，制得波尔多液； Put copper sulfate into water to dilute, and the mass ratio of copper sulfate to water is 1:80, after stirring evenly, make diluted copper sulfate, put quicklime into water, and the mass ratio of quicklime to water is 1:40, stir After uniformity, the lime milk is obtained, and the diluted copper sulfate is poured into the lime milk, and stirred while pouring to obtain the Bordeaux mixture;
步骤三、将所述的枯草芽孢杆菌HS5B5、生物肥和波尔多液混合后搅拌均匀即制得生物农药，且生物农药中各成分的重量份数为枯草芽孢杆菌HS5B5的重量份数为1-2份，生物肥的重量份数为8-10份，波尔多液的重量份数为4-5份。 Step 3, mixing the Bacillus subtilis HS5B5, biological fertilizer and Bordeaux mixture and stirring evenly to obtain a biopesticide, and the parts by weight of each component in the biopesticide are 1-2 parts by weight of the Bacillus subtilis HS5B5 The parts by weight of biological fertilizer are 8-10 parts, and the parts by weight of Bordeaux mixture are 4-5 parts.
实施例1 Example 1
一种用于防治牡丹褐斑病的生物农药，所述生物农药由枯草芽孢杆菌、生物肥和波尔多液制成，且制备过程所用的各组分重量份数分别为：枯草芽孢杆菌2份，生物肥10份和波尔多液5份，所述枯草芽孢杆菌命名为枯草芽孢杆菌HS5B5。 A biopesticide for preventing and treating brown spot of peony, said biopesticide is made of Bacillus subtilis, biological fertilizer and Bordeaux mixture, and the parts by weight of each component used in the preparation process are: 2 parts of Bacillus subtilis, 10 parts of biological fertilizer and 5 parts of Bordeaux mixture, and the bacillus subtilis is named as bacillus subtilis HS5B5.
所述的生物农药的制备方法，包括以下步骤： The preparation method of described biopesticide, comprises the following steps:
步骤一、所述生物肥的制备 Step 1, the preparation of the biological fertilizer
在牲畜粪便中加入酵母菌，且牲畜粪便与酵母菌质量的比例为500:1，搅拌均匀后放入发酵室内发酵，7天后取出，制得混合物，向混合物中加入菜籽饼，且混合物与菜籽饼的质量比例为2:1，搅拌均匀后晾晒，制得生物肥； Yeast is added to the livestock manure, and the ratio of the mass of the livestock manure to the yeast is 500:1, stirred evenly, put into the fermentation room for fermentation, and taken out after 7 days to prepare a mixture, and rapeseed cake is added to the mixture, and the mixture is mixed with The mass ratio of the rapeseed cake is 2:1, and after being stirred evenly, it is dried in the air to obtain a biological fertilizer;
步骤二、所述波尔多液的制备 Step 2, the preparation of the Bordeaux mixture
将硫酸铜放入水中稀释，且硫酸铜与水的质量比例为1:80，搅拌均匀后，制得稀释硫酸铜，将生石灰放入水中，且生石灰和水的质量比例为1:40，搅拌均匀后，制得石灰乳，将稀释硫酸铜倒入石灰乳中，边倒边搅拌，制得波尔多液； Put copper sulfate into water to dilute, and the mass ratio of copper sulfate to water is 1:80, after stirring evenly, make diluted copper sulfate, put quicklime into water, and the mass ratio of quicklime to water is 1:40, stir After uniformity, the lime milk is obtained, and the diluted copper sulfate is poured into the lime milk, and stirred while pouring to obtain the Bordeaux mixture;
步骤三、将所述的枯草芽孢杆菌HS5B5、生物肥和波尔多液混合后搅拌均匀即制得生物农药，且生物农药中各成分的重量份数为枯草芽孢杆菌HS5B5的重量份数为2份，生物肥的重量份数为10份，波尔多液的重量份数为5份。 Step 3, mixing the Bacillus subtilis HS5B5, biological fertilizer and Bordeaux mixture and stirring evenly to obtain a biopesticide, and the parts by weight of each component in the biopesticide are 2 parts by weight of the Bacillus subtilis HS5B5, The parts by weight of the biological fertilizer are 10 parts, and the parts by weight of the Bordeaux mixture are 5 parts.
实施例2 Example 2
一种用于防治牡丹褐斑病的生物农药，所述生物农药由枯草芽孢杆菌、生物肥和波尔多液制成，且制备过程所用的各组分重量份数分别为：枯草芽孢杆菌1份，生物肥8份和波尔多液4份，所述枯草芽孢杆菌命名为枯草芽孢杆菌HS5B5。 A biopesticide for preventing and treating brown spot of peony, said biopesticide is made of Bacillus subtilis, biological fertilizer and Bordeaux mixture, and the parts by weight of each component used in the preparation process are: 1 part of Bacillus subtilis, 8 parts of biological fertilizer and 4 parts of Bordeaux mixture, and the bacillus subtilis is named as bacillus subtilis HS5B5.
所述的生物农药的制备方法，包括以下步骤： The preparation method of described biopesticide, comprises the following steps:
步骤一、所述生物肥的制备 Step 1, the preparation of the biological fertilizer
在牲畜粪便中加入酵母菌，且牲畜粪便与酵母菌质量的比例为500:1，搅拌均匀后放入发酵室内发酵，6天后取出，制得混合物，向混合物中加入菜籽饼，且混合物与菜籽饼的质量比例为2:1，搅拌均匀后晾晒，制得生物肥； Yeast is added to the livestock manure, and the ratio of the mass of the livestock manure to the yeast is 500:1, stirred evenly, put into the fermentation room for fermentation, taken out after 6 days, and the mixture is prepared, and rapeseed cake is added to the mixture, and the mixture is mixed with The mass ratio of the rapeseed cake is 2:1, and after being stirred evenly, it is dried in the air to obtain a biological fertilizer;
步骤二、所述波尔多液的制备 Step 2, the preparation of the Bordeaux mixture
将硫酸铜放入水中稀释，且硫酸铜与水的质量比例为1:80，搅拌均匀后，制得稀释硫酸铜，将生石灰放入水中，且生石灰和水的质量比例为1:40，搅拌均匀后，制得石灰乳，将稀释硫酸铜倒入石灰乳中，边倒边搅拌，制得波尔多液； Put copper sulfate into water to dilute, and the mass ratio of copper sulfate to water is 1:80, after stirring evenly, make diluted copper sulfate, put quicklime into water, and the mass ratio of quicklime to water is 1:40, stir After uniformity, the lime milk is obtained, and the diluted copper sulfate is poured into the lime milk, and stirred while pouring to obtain the Bordeaux mixture;
步骤三、将所述的枯草芽孢杆菌HS5B5、生物肥和波尔多液混合后搅拌均匀即制得生物农药，且生物农药中各成分的重量份数为枯草芽孢杆菌HS5B5的重量份数为1份，生物肥的重量份数为8份，波尔多液的重量份数为4份。 Step 3, mixing the Bacillus subtilis HS5B5, biological fertilizer and Bordeaux mixture and stirring evenly to obtain the biopesticide, and the parts by weight of each component in the biopesticide is 1 part by weight of the Bacillus subtilis HS5B5, The parts by weight of the biological fertilizer are 8 parts, and the parts by weight of the Bordeaux mixture are 4 parts.
试验一 test one
2015年4月6日，选取洛阳国际牡丹园牡丹栽培区的400株牡丹作为试验对象，将牡丹均分为A、B、C、D四组，且对A、B、C、D四中的牡丹苗接种等量的牡丹枝孢霉。A组使用本发明的生物农药，每隔两周喷洒一次；B组使用本发明中的枯草芽孢杆菌HS5B5菌液，每隔两周喷洒一次；C组使用本发明除去枯草芽孢杆菌HS5B5后的生物农药，每隔两周喷洒一次；D组每隔两周喷洒一次水，作为对照。 On April 6, 2015, 400 peonies in the peony cultivation area of Luoyang International Peony Garden were selected as test subjects, and the peonies were divided into four groups A, B, C, and D, and the Peony seedlings were inoculated with the same amount of Cladosporium peony. Group A uses the biopesticide of the present invention and sprays once every two weeks; Group B uses the Bacillus subtilis HS5B5 bacterium liquid of the present invention and sprays it once every two weeks; Pesticides were sprayed every two weeks; group D was sprayed with water every two weeks as a control.
2015年6月5日检查结果：A组中无牡丹褐斑病和牡丹白粉病发生，植株生长健壮，B组有6株牡丹发生褐斑病，有17株牡丹发生白粉病，植株明显不如A组健壮，C组中32株牡丹发生褐斑病，2株牡丹发生白粉病，植株虽不如A组健壮但明显比B组健壮，D组中79株牡丹发生褐斑病，同时14株牡丹发生白粉病，植株生长弱小。 Inspection results on June 5, 2015: In group A, no peony brown spot and tree peony powdery mildew occurred, and the plants grew robustly. In group B, 6 peony plants had brown spot disease, and 17 peony plants had powdery mildew. The plants were obviously not as good as A In group C, 32 peonies suffered from brown spot and 2 peonies suffered from powdery mildew. Although the plants were not as strong as in group A, they were obviously stronger than group B. In group D, 79 peonies suffered from brown spot, and 14 peonies suffered from brown spot. Powdery mildew, weak plant growth.
结果表明：枯草芽孢杆菌HS5B5能够预防牡丹褐斑病，同时对牡丹白粉病也有一定的抑制作用，生物肥和波尔多液混合使用能够提高土壤肥力，刺激植物生长，促进牡丹对养分的吸收，对预防牡丹褐斑病和牡丹白粉病虽有一定的作用但效果不明显，而本发明的生物农药不仅能够更加有效的预防牡丹褐斑病，而且能够有效的预防牡丹白粉病，同时还可以促进植株的生长。 The results show that: Bacillus subtilis HS5B5 can prevent brown spot of tree peony, and also has a certain inhibitory effect on powdery mildew of tree peony. The mixed use of biological fertilizer and Bordeaux mixture can improve soil fertility, stimulate plant growth, and promote the absorption of nutrients by tree peony. Although peony brown spot and tree peony powdery mildew have certain effects, the effect is not obvious, and the biopesticide of the present invention can not only prevent tree peony brown spot more effectively, but also can effectively prevent tree peony powdery mildew, and can also promote plant growth and development. grow.
试验二 Test two
选取300株长势相同的牡丹，均分为A、B、C三组，其中A组使用本发明的生物农药，B组使用本发明中的生物肥，C组不添加任何成分。观察并记录生长情况。 Select 300 peonies with the same growth potential and divide them into three groups: A, B, and C, wherein group A uses the biological pesticide of the present invention, group B uses the biological fertilizer of the present invention, and group C does not add any ingredients. Observe and record growth.
试验结果：A组平均整个花期为11天，B组平均整个花期9天，C组平均整个花期为6天。 Test results: The average flowering period of group A is 11 days, the average flowering period of group B is 9 days, and the average flowering period of group C is 6 days.
结果表明：生物肥能够起到延长牡丹花期的作用，加入枯草芽孢杆菌HS5B5和波尔多液形成本发明的生物农药后对牡丹花期的延长作用更加明显。 The results show that the biological fertilizer can prolong the flowering period of peony, and the effect of prolonging the flowering period of peony is more obvious after adding Bacillus subtilis HS5B5 and Bordeaux mixture to form the biological pesticide of the present invention.
试验一和试验二的结果表明，枯草芽孢杆菌HS5B5通对牡丹枝孢霉为病原真菌的牡丹褐斑病有明显的抑制效果，对牡丹白粉病也有一定抑制效果，生物肥和波尔多液对牡丹白粉病的抑制和植物促生、延长花期上有明显效果。通过比例混合后，本发明的生物农药能够抑制牡丹褐斑病和牡丹白粉病病原真菌的活动，增加牡丹的抗病力和抗逆性，促进植株生长，它的作用和效果不仅仅是枯草芽孢杆菌、生物肥和波尔多液的简单累加，复配后各成分之间还有明显的协同增效作用。 The results of Experiment 1 and Experiment 2 show that Bacillus subtilis HS5B5 has obvious inhibitory effect on tree peony brown spot with Cladosporium peony as the pathogenic fungus, and also has a certain inhibitory effect on tree peony powdery mildew. It has obvious effects on disease suppression, plant growth promotion and flowering extension. After being mixed in proportion, the biopesticide of the present invention can inhibit the activity of pathogenic fungi of peony brown spot and peony powdery mildew, increase the disease resistance and stress resistance of peony, and promote plant growth. The simple accumulation of bacillus, biological fertilizer and Bordeaux mixture, after compounding, there is an obvious synergistic effect between the ingredients.
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