CN113088461A - Microbial compound inoculant and application thereof - Google Patents
Microbial compound inoculant and application thereof Download PDFInfo
- Publication number
- CN113088461A CN113088461A CN201911342257.4A CN201911342257A CN113088461A CN 113088461 A CN113088461 A CN 113088461A CN 201911342257 A CN201911342257 A CN 201911342257A CN 113088461 A CN113088461 A CN 113088461A
- Authority
- CN
- China
- Prior art keywords
- microbial
- bacillus
- inoculant
- microbial inoculum
- plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明公开了一种微生物复合菌剂及其应用。本发明首先公开了一种微生物复合菌剂，所述微生物复合菌剂的活性成分由解淀粉芽孢杆菌和菌种A组成；所述菌种A为短波单胞菌、假单胞菌、芽孢杆菌或不动杆菌。本发明进一步公开了所述微生物复合菌剂在在促植物生长中的应用及促植物生长的方法。本发明通过将解淀粉芽孢杆菌FH‑1与其他7株细菌按照菌落形成单位数目比1∶1的比例复配成微生物复合菌剂，利用盆栽实验筛选除了具有高效促水稻根长、苗长、鲜重和干重的微生物复合菌剂FN，能显著促进水稻的生长和抑制水稻病原菌的产生。The invention discloses a microbial compound bacterial agent and its application. The invention firstly discloses a microbial compound microbial inoculant. The active components of the microbial compound microbial inoculant are composed of Bacillus amyloliquefaciens and strain A; the strain A is Brevimonas, Pseudomonas, and Bacillus or Acinetobacter. The invention further discloses the application of the microbial compound inoculant in promoting plant growth and a method for promoting plant growth. In the present invention, Bacillus amyloliquefaciens FH-1 and other 7 strains of bacteria are compounded into a microbial compound bacterial agent according to the ratio of the number of colony forming units to 1:1. Fresh weight and dry weight microbial compound inoculant FN can significantly promote the growth of rice and inhibit the production of rice pathogens.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a microbial compound inoculant and application thereof.
Background
At present, excessive use of chemical fertilizers and pesticides not only causes pollution of a series of ecological environments, but also seriously harms the health of people and sustainable development of agriculture and society. The microbial agent is a green, environment-friendly and eco-friendly fertilizer, has the characteristics of increasing soil fertility, reducing the use of chemical fertilizers and pesticides, purifying and repairing soil, reducing plant diseases, improving quality and yield, improving food safety and the like, and is an important way for realizing sustainable development of agriculture. Microbial agents have been widely used worldwide.
The single microbial agent has the problems of single function, poor adaptability and the like. Different functional strains are combined to obtain a microbial compound microbial inoculum which has stronger growth promoting capability and more stability than a single microbial inoculum, and the microbial compound microbial inoculum is a development trend of the microbial inoculum. At present, the research of the microbial compound bacteria has entered the industrialization stage, and is focused by biological agriculture companies at home and abroad. For example, the M-14 microbial compound microbial agent is applied to Wangjun Wen (Wangjun Wen, Zhao Jun Wei, Liuli, etc.. M-14 compound biological fertilizer for resisting wheat cyst nematode and increasing the yield [ J ] in the Chinese biological prevention and control bulletin 2016, 32 (5): 676 680), so that the yield of the wheat is increased by 14.3 percent and the infection rate of the cyst nematode is reduced by 43.4 to 70.8 percent. TJ Technology company develops a microbial compound microbial inoculum named as QuickRoots, which can improve the nutrient element supply of crops in the seedling stage, and the yield of the crops can reach about 220-; TJ Technology corporation assigned the Technology to monsanto in 2014 at a price of 3 billion dollars, this example is sufficient to highlight the application potential and market size of microbial complex agents.
Disclosure of Invention
The invention aims to solve the technical problem of obtaining a microbial composite inoculant with growth promoting property.
In order to solve the technical problems, the invention firstly provides a microbial compound inoculant.
The active ingredients of the microbial compound inoculant consist of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and a strain A; the strain A is Brevundimonas sp, Pseudomonas sp, Bacillus sp or Acinetobacter sp.
In the microbial composite inoculant, the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FH-1;
the Brevundimonas sp is Brevundimonas sp N-YM-3;
the Pseudomonas (Pseudomonas sp.) is Pseudomonas (Pseudomonas sp.) G3-6;
the Bacillus (Bacillus sp.) is Bacillus cereus (LSI-1), Bacillus (Bacillus sp.) JFH-1 or Bacillus (Bacillus sp.) IF-2 or Bacillus (Bacillus sp.) ZPC-1;
the Acinetobacter (Acinetobacter sp.) is Acinetobacter (Acinetobacter sp.) KYM-3.
In the microbial compound bacterial agent, the number ratio of colony forming units (cfu) of bacillus amyloliquefaciens and the strain A in the active ingredients of the microbial compound bacterial agent is 1: 1.
The invention further provides an application of the microbial compound inoculant in any one of the following steps:
A1) the application in promoting the growth of plants;
A2) the application in improving the plant seedling length;
A3) the application in improving the root length of plants;
A4) the application in improving the fresh weight of plants;
A5) use in increasing the dry weight of plants.
A6) The application in inhibiting plant pathogenic bacteria.
The invention also provides a method for promoting plant growth by using the microbial compound inoculant.
The method for promoting the growth of the plants comprises the step of applying the microbial compound inoculant to the soil for planting the plants so as to promote the growth of the plants.
In the method, the applied dosage of the microbial compound inoculant is 1 multiplied by 10 per gram (g) of soil8cfu。
Herein, the plant is any one of:
B1) a monocot plant;
B2) a dicotyledonous plant;
B3) plants of the genus Oryza;
B4) a rice plant.
According to the invention, Bacillus amyloliquefaciens (FH-1) and other 7 strains of bacteria are compounded into the microbial compound inoculant according to the ratio of the number of colony forming units (cfu) to 1: 1, and a pot experiment is utilized to screen the microbial compound inoculant FN which has the advantages of high efficiency in promoting the root length, seedling length, fresh weight and dry weight of rice, can obviously promote the growth of rice and has the capability of inhibiting pathogenic bacteria of rice.
Drawings
FIG. 1 shows the growth effect of different microbial inoculum on rice seedling length (A), root length (B), fresh weight (C) and dry weight (D).
FIG. 2 is a graph showing the growth of rice.
FIG. 3 shows the growth effect of different microbial agents on dry weight (A), fresh weight (B), seedling length and root length (C) of rice.
FIG. 4 shows the inhibiting effect of different microbial inoculum on crop pathogenic bacteria.
FIG. 5 shows the inhibition rates of different microbial agents on crop pathogens.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures used in the following examples are all conventional procedures unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The source of each test strain in the following examples of the invention:
bacillus amyloliquefaciens FH-1 (F for short) is described in the growth promoting mechanism of rhizosphere microbiome-mediated Bacillus amyloliquefaciens FH-1 on rice [ J/OL ] microbiology report;
brevundimonas sp N-YM-3 (N for short) is described in "Song Xu et al isolation and potential of Ochrobactrum sp.NW-3to increment the growth of culture, International Journal of Agricultural polarization and Research, 2016";
pseudomonas sp G3-6 (G for short) is described in "huan juanhuan, king jing, xu song, et al, screening of organophosphorus degrading bacteria and its growth promoting property [ J ] microbiology report 2017, 57 (5): 47-60';
bacillus cereus LSI-1 (L for short), Bacillus sp JFH-1 (J for short), Bacillus sp IF-2 (I for short), Bacillus sp ZPC-1 (Z for short) and Acinetobacter (Acinetobacter sp) KYM-3 (K for short) are screened and preserved in microbiological ecological engineering laboratories applied to Tianjin industrial biotechnology research institute of Chinese academy of sciences.
Fusarium graminearum F679 is described in "Nile, xu Song, Zhaowei, et al. rhizosphere microbiome mediated growth promoting mechanism of Bacillus amyloliquefaciens FH-1 on rice [ J/OL ]. Microbiol.
In the following examples of the present invention, the soil to be tested was cultivated land soil of Huaming town of east Li district, Tianjin, and the soil type was yellow soil. The soil properties are: 1.78% of organic matters; 3.00g/kg of total nitrogen, 0.39g/kg of total phosphorus and 5.13g/kg of total potassium; quick-acting nitrogen 37.33 mg/kg; 9.57mg/kg of quick-acting phosphorus; quick-acting potassium is 61.84 mg/kg; pH 7.69.
The test crop in the following examples of the present invention was rice (Nei 5 you 8015 hybrid rice) purchased from Zhejiang agricultural species Co., Ltd.
In the following examples of the present invention, LB liquid medium, inorganic phosphorus medium, organic phosphorus medium, potassium-solubilizing medium, CAS medium, Achillea fritillary nitrogen-fixing medium, PDA medium, CAS stain and Salkowski's color-developing solution are described in "Nile, Xusong, Zhaowei, et al, growth promoting mechanism [ J/OL ] microbiology report of rhizosphere microbiome-mediated Bacillus amyloliquefaciens FH-1 for rice; screening of organophosphorus degrading bacteria and growth promoting characteristics thereof [ J ] microbiological report, 2017, 57 (5): 47-60 and baiting, wangjing, willow shadow, and the like, screening of high-efficiency phosphate solubilizing bacteria and preliminary research on growth promoting mechanism thereof [ J ] biotechnological report 2015, 31 (12): 234-242".
SPX-250B5 Biochemical incubator, Shanghai New Yong medical device manufacturing Co., Ltd; UV-1800 UV-Vis Spectrophotometer, Shimadzu, Japan; RXZ-500B-LED artificial intelligence climate incubator, instrument factory in south of the Yangtze river.
All data in the following examples of the invention are mean ± standard deviation. The difference significance of the length, the root length, the fresh weight and the dry weight of rice seedlings in each basin among different microbial inoculums is analyzed through t test (P is less than or equal to 0.05). All statistical analyses were performed using IBM SPSS static (Version21.0) and all mapping was done using Origin 2016.
Example 1 screening of composite microbial inoculum
First, culture of the strains
Strains of Bacillus amyloliquefaciens FH-1 (F for short), Bacillus cereus LSI-1 (L for short), Bacillus JFH-1 (J for short), Bacillus (I sp) IF-2 (I for short), Bacillus (Z) ZPC-1 (Z for short), Brevundimonas (G) N-YM-3 (N for short), Acinetobacter (Acinetobacter sp) KYM-3 (K for short) and Pseudomonas (Pseudomonas sp) G3-6 (G for short) which are preserved at-80 ℃ are respectively inoculated into LB liquid culture medium according to the proportion of 5 percent and are cultured by a shaker at 30 ℃ for 24 hours in an activated manner of 180 r/min.
Second, screening experiment of rice pot culture
1. Preparation of complex microbial inoculum
After each strain is activated for 24 hours, the strain is cultured in LB culture medium for 60 hours in an inoculation amount of 5 percent respectively, and then the micro counting is carried out, and the strain is diluted to 8 multiplied by 10 by tap water8cfu/mL bacterial liquids (diluted about 250 times) to respectively obtain F bacterial liquids (Bacillus amyloliquefaciens in the F bacterial liquids)The content of s amyloliquefaciens) FH-1 is 8X 108cfu/mL), L bacterial liquid (the content of Bacillus cereus LSI-1 in the L bacterial liquid is 8X 108cfu/mL), J bacterial liquid (Bacillus sp in J bacterial liquid) with JFH-1 content of 8 × 108cfu/mL), the content of the I bacterial liquid (Bacillus sp) IF-2 in the I bacterial liquid is 8 multiplied by 108cfu/mL), Z bacterial liquid (the content of Bacillus sp ZPC-1 in the Z bacterial liquid is 8 multiplied by 10)8cfu/mL), N bacterial liquid (Brevundimonas sp. in the N bacterial liquid), N-YM-3 content is 8 × 108cfu/mL), K bacterial liquid (Acinetobacter sp in K bacterial liquid) with a content of 8 × 10 KYM-38cfu/mL), G bacterial liquid (Pseudomonas sp in G bacterial liquid) with the content of G3-6 being 8 × 108cfu/mL), the composite microbial inoculum is 15mL F after dilution and 15mL other bacterial liquids, 9 treatment groups are arranged in total (each treatment group is provided with 4 repetitions): blank control group (CK) and 8 inoculum groups. The blank (CK) group was treated with tap water. 8 microbial inoculum groups are treated by corresponding microbial inocula. The microbial inoculum group F is treated by a microbial inoculum F, the 30mL of the microbial inoculum F is 30mL of F bacterial liquid, and the content of Bacillus amyloliquefaciens FH-1 in the F bacterial liquid is 8 multiplied by 108cfu/mL. The microbial inoculum group FJ is treated by a composite microbial inoculum FJ, 30mL of the composite microbial inoculum FJ consists of 15mL of F bacterial liquid and 15mL of J bacterial liquid, and the content of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FH-1 in the composite microbial inoculum FJ is 8 multiplied by 108cfu/mL, the content of Bacillus sp JFH-1 is 8 multiplied by 108cfu/mL; the microbial inoculum group FL is treated by a composite microbial inoculum FL, 30mL of the composite microbial inoculum FL consists of 15mL of F bacterial liquid and 15mL of L bacterial liquid, and the content of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FH-1 in the composite microbial inoculum FL is 8 multiplied by 108cfu/mL, the content of Bacillus cereus LSI-1 is 8X 108cfu/mL; the microbial inoculum group FJ is treated by a composite microbial inoculum FI, 30mL of the composite microbial inoculum FI consists of 15mL of F bacterial liquid and 15mL of I bacterial liquid, and the content of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FH-1 in the composite microbial inoculum FI is 8 multiplied by 108cfu/mL, the content of Bacillus sp IF-2 is 8 × 108cfu/mL; the bacterium agent group FZ is treated by adopting a compound bacterium agent FZ, and 30mL of the compound bacterium agent FZ is prepared from 15mL of F bacterium liquid and 15mL of Z bacteriumThe liquid composition is that the content of Bacillus amyloliquefaciens FH-1 in the composite bacterial agent FZ is 8 multiplied by 108cfu/mL, the content of ZPC-1 of Bacillus (Bacillus sp.) is 8 x 108cfu/mL; the microbial inoculum group FN is treated by composite microbial inoculum FN, 30mL of composite microbial inoculum FN consists of 15mL of F bacterial liquid and 15mL of N bacterial liquid, and the content of Bacillus amyloliquefaciens FH-1 in the composite microbial inoculum FN is 8 × 108cfu/mL, the content of Brevundimonas sp N-YM-3 is 8 x 108cfu/mL; the bacterial agent group FK is treated by a compound bacterial agent FK, 30mL of the compound bacterial agent FK consists of 15mL of F bacterial liquid and 15mL of K bacterial liquid, and the content of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FH-1 in the compound bacterial agent FK is 8 multiplied by 108cfu/mL, the content of Acinetobacter (Acinetobacter sp.) KYM-3 is 8 multiplied by 108cfu/mL; the bacterium agent group FG is treated by a composite bacterium agent FG, 30mL of the composite bacterium agent FG consists of 15mL of F bacterium liquid and 15mL of G bacterium liquid, and in the composite bacterium agent FG, the content of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FH-1 is 8 multiplied by 108cfu/mL, the content of Pseudomonas sp G3-6 is 8X 108cfu/mL。
2. Screening experiment of complex microbial inoculum
Putting the rice seeds into distilled water, standing at 30 ℃ and incubating for 24h, transferring the rice seeds into wet gauze, and incubating for 24h at 30 ℃ until the rice seeds are all exposed to the white. The soil to be tested is dried in a shade and light-resistant place, the soil to be tested is sieved by 5mm, 240g of the soil to be tested is filled into flowerpots with the diameter of 8 cm, 13 rice seeds with white exposure are sown in each flowerpot, 1 seedling appears in 5d of sowing, the seedlings with poor growth and flowerpots are removed, and finally 11 seedlings with basically uniform growth vigor in each flowerpot are kept. Then applying 30mL of corresponding microbial inoculum to each flowerpot according to the 8 microbial inoculum groups set in the step 1 to meet the requirement of 1 multiplied by 108cfu/g soil, blank control 30mL of tap water was applied per pot. Each treatment was set with 4 pots. Before seedling emergence, controlling the temperature in the incubator at 30 ℃, keeping the 1 leaf exposed tip at 28 ℃ in the daytime and 24 ℃ at night; keeping the leaf stage at 25 ℃, keeping the leaf stage at 22 ℃, keeping the illumination time of 1 day at 16h, keeping the humidity of the incubator at 75%, and carrying out unified management (watering 30mL every 48h, and randomly switching the positions of flowerpots). Sample collection and characterization of plantsAnd (3) analysis: the collection time of the rice pot experiment sample is about 16 days after planting, and the plant is pulled out for subsequent measurement under the condition of not damaging the root and the seedling of the plant. The fresh weight and the dry weight of each pot of rice seedlings are measured by a balance, and the seedling height and the root length of each pot of rice seedlings are measured by a ruler.
As shown in fig. 1, through comprehensive analysis of the data of seedling length (a), root length (B), fresh weight (C) and dry weight (D) of each pot of rice, it was found that, compared with CK, the microbial inoculum group FN significantly increased the seedling length of rice by 20.79%, the root length by 26.67% and the fresh weight by 74.84%, the microbial inoculum group FL significantly increased the fresh weight of rice by 68.23% and the dry weight by 43.14%, the microbial inoculum groups FJ, FK and FG significantly increased the fresh weights of rice by 61.19%, 75.48% and 63.75%, respectively, and the microbial inoculum group FZ significantly increased the dry weight of rice by 42.48% (P ≦ 0.05). Compared with other treatment groups, the microbial inoculum group FN can improve the rice seedling length by 7.12-15.47% and the root length by 1.64-18.82%. Except for the microbial inoculum group FK, compared with other treatment groups, the microbial inoculum group FN can improve the fresh weight of rice by 3.93-32.90%. In addition to the microbial inoculum groups FL and FZ, the microbial inoculum group FN can increase the dry weight of rice by 3.48-17.51% compared with other treatment groups. In addition, the seedling length of the microbial inoculum group FI is lower than that of the microbial inoculum group F, and the root length of the microbial inoculum group FJ is lower than that of the microbial inoculum group F.
In combination with the above, the compound microbial inoculum FN is a microbial compound microbial inoculum with a better comprehensive effect in 7 kinds of microbial compound microbial inocula.
Thirdly, verifying the capability of the composite microbial inoculum FN in promoting the growth of rice
The ability of the composite microbial inoculum FN to promote the growth of rice is further verified by expanding the repetition among treatment groups.
1. Preparation of microbial inoculum
Activating the F bacterial liquid and the N bacterial liquid for 24 hours respectively, culturing the activated F bacterial liquid and the N bacterial liquid in an LB culture medium for 60 hours in an inoculation amount of 5 percent respectively, and diluting the activated F bacterial liquid and the N bacterial liquid to 8 x 10 by using tap water8CFU/mL bacterial liquid (diluted about 250 times) to respectively obtain F bacterial liquid (Bacillus amyloliquefaciens FH-1 in the F bacterial liquid) with the content of 8 × 108cfu/mL) and N bacterial liquid (Brevundimonas sp. in the N bacterial liquid), wherein the content of N-YM-3 is 8 multiplied by 108cfu/mL), 4 treatment groups were set up (9 replicates per treatment group): blank control group (CK) and 3 inoculum groups. Blank pairThe Column (CK) was treated with tap water. 8 microbial inoculum groups are treated by corresponding microbial inocula. The microbial inoculum group F is treated by a microbial inoculum F, the 30mL of the microbial inoculum F is 30mL of F bacterial liquid, and the content of Bacillus amyloliquefaciens FH-1 in the F bacterial liquid is 8 multiplied by 108cfu/mL; the microbial inoculum group FN is treated by composite microbial inoculum FN, 30mL of composite microbial inoculum FN consists of 15mL of F bacterial liquid and 15mL of N bacterial liquid, and the content of Bacillus amyloliquefaciens FH-1 in the composite microbial inoculum FN is 8 × 108cfu/mL, the content of Brevundimonas sp N-YM-3 is 8 x 108cfu/mL; the bacterium agent group N is treated by a bacterium agent N, 30mL of the bacterium agent N is 30mL of N bacterium liquid, and the content of Brevundimonas sp N-YM-3 in the N bacterium liquid is 8 multiplied by 108cfu/mL。
2. Capability test of microbial inoculum for promoting growth of rice
Putting the rice seeds into distilled water, standing at 30 ℃ and incubating for 24h, transferring the rice seeds into wet gauze, and incubating for 24h at 30 ℃ until the rice seeds are basically completely exposed. And after rice is exposed, filling 240g of soil to be tested into flowerpots with the diameter of 8 cm, putting a proper amount of rice seeds in each flowerpot, sowing 1 leaf of seedlings in 5 days, removing the seedlings with poor growth and the flowerpots, and finally keeping 11 seedlings with basically uniform growth vigor in each flowerpot. Then applying 30mL of corresponding microbial inoculum to each flowerpot according to the 4 microbial inoculum groups set in the step 1 to meet the requirement of 1 multiplied by 108cfu/g soil, blank control 30mL of tap water was applied per pot. Each treatment was set with 9 pots. Before seedling emergence, controlling the temperature in the incubator at 30 ℃, keeping the 1 leaf exposed to the tip at 28 ℃ in the daytime and keeping the temperature at 24 ℃ at night; keeping the leaf stage at 25 deg.C and leaf stage at 22 deg.C for 2 deg.C, lighting for 16h in 1 day, and watering soil with incubator humidity of 75%. Sample collection and plant characteristic analysis: the collection time of the rice pot experiment sample is about 16 days after planting, under the condition of not damaging the roots and stems of the plants, the plants are pulled out for subsequent measurement, and the result is shown in figure 2. The fresh weight and the dry weight of the rice seedlings are measured by a balance, and the seedling height and the root length of the rice seedlings are measured by a ruler.
As shown in FIG. 3, A, B, C in FIG. 3 are indicators of the length of rice seedlings on the ground (seedling length), the length of the ground (root length), the fresh weight and the dry weight, respectively. The seedling length of the microbial inoculum group FN, the microbial inoculum group F and the microbial inoculum group N is respectively increased by 32.11 percent, 19.25 percent and 23.00 percent compared with that of CK; compared with CK, the root lengths of the microbial inoculum group FN, the microbial inoculum group F and the microbial inoculum group N are respectively increased by 77.09%, 46.05% and 35.42%. Compared with the overground fresh weight of CK, the microbial inoculum groups FN, F and N are respectively increased by 130.57%, 89.22% and 93.82%; compared with CK, underground fresh weights of the microbial inoculum group FN, the microbial inoculum group F and the microbial inoculum group N are respectively increased by 202.70%, 101.47% and 80.64%. The overground dry weights of the fungicide group FN, the fungicide group F and the fungicide group N are increased by 198.00%, 149.00% and 163.27% respectively compared with the overground dry weights of CK; compared with the underground dry weight of CK, the underground dry weights of the microbial inoculum group FN, the microbial inoculum group F and the microbial inoculum group N are respectively increased by 94.60%, 61.26% and 52.25%.
By combining the indexes above ground and underground, it can be obviously seen that the composite microbial inoculum FN obviously promotes the growth of rice seedlings, and is obviously superior to a single microbial inoculum.
Fourth, the ability of the composite bacterial agent FN to inhibit plant pathogenic bacteria
After the F and N bacterial liquids are activated for 24 hours respectively, the bacterial liquids are cultured in an LB culture medium for 24 hours respectively in an inoculation amount of 5 percent. Activating crop pathogenic bacteria Fusarium graminearum F679 on a PDA plate for 3 days, transferring to a new PDA plate, simultaneously sucking 5 μ L of bacterial liquid F, N and FN cultured for 24h by using a pipette gun, respectively inoculating on the PDA plate, standing at 30 deg.C for 4 days to observe whether there is antagonism, and setting 4 repeats for each treatment group with non-inoculated bacteria as control. Antagonistic capacity determination formula:
percent inhibition (%) as (control fungus diameter-treated fungus diameter) ÷ control fungus diameter × 100%
As shown in fig. 4 and 5, the bacteriostatic ratio of the inoculum group FN was 65.44%, the bacteriostatic ratio of the inoculum group F was 62.75%, and the bacteriostatic ratios of the inoculum groups N and CK were 0%. Compared with the microbial inoculum group F, the bacteriostatic rate of the microbial inoculum group FN is increased by 4.29 percent.
In conclusion, the composite microbial inoculum FN can obviously inhibit crop pathogenic bacteria and is superior to a single microbial inoculum.
The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.
Claims (10)
1. A compound microbial inoculum is characterized in that: the active ingredients of the microbial compound inoculant consist of bacillus amyloliquefaciens and a strain A; the strain A is brevundimonas, pseudomonas, bacillus or acinetobacter.
2. The complex microbial inoculant according to claim 1, wherein: the bacillus amyloliquefaciens is bacillus amyloliquefaciens FH-1.
3. The complex microbial inoculant according to claim 1, wherein: the brevundimonas is brevundimonas N-YM-3; the pseudomonas is pseudomonas G3-6;
the bacillus is bacillus cereus LSI-1, bacillus JFH-1, bacillus IF-2 or bacillus ZPC-1;
the acinetobacter is acinetobacter KYM-3.
4. The complex microbial inoculant according to any one of claims 1 to 3, wherein: the colony forming unit number ratio of the bacillus amyloliquefaciens to the strain A in the active ingredients of the microbial compound inoculant is 1: 1.
5. The use of a complex microbial inoculant according to any one of claims 1 to 4 in any one of:
A1) the application in promoting the growth of plants;
A2) the application in improving the plant seedling length;
A3) the application in improving the root length of plants;
A4) the application in improving the fresh weight of plants;
A5) use in increasing the dry weight of plants;
A6) the application in inhibiting plant pathogenic bacteria.
6. A method of promoting plant growth, comprising: the method comprises applying the microbial composite inoculum according to any one of claims 1 to 4 to the soil in which plants are grown to promote the growth of the plants.
7. The method of claim 6, wherein: the applied dosage of the microbial compound inoculant is 1 multiplied by 10 per gram of soil8cfu。
8. The use according to claim 5, or the method according to claim 6 or 7, wherein: the plant is any one of the following plants:
B1) a monocot plant;
B2) dicotyledonous plants.
9. The use or method according to claim 8, wherein: the monocotyledon is a plant of the genus Oryza.
10. The use or method according to claim 9, wherein: the Oryza plant is rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911342257.4A CN113088461A (en) | 2019-12-23 | 2019-12-23 | Microbial compound inoculant and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911342257.4A CN113088461A (en) | 2019-12-23 | 2019-12-23 | Microbial compound inoculant and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113088461A true CN113088461A (en) | 2021-07-09 |
Family
ID=76663973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911342257.4A Pending CN113088461A (en) | 2019-12-23 | 2019-12-23 | Microbial compound inoculant and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113088461A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635903A (en) * | 2016-12-23 | 2017-05-10 | 河北省农林科学院遗传生理研究所 | Growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands |
| CN108925586A (en) * | 2018-08-24 | 2018-12-04 | 成都特普生物科技股份有限公司 | A kind of antimicrobial composition preparation improving plant pest management effect |
-
2019
- 2019-12-23 CN CN201911342257.4A patent/CN113088461A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635903A (en) * | 2016-12-23 | 2017-05-10 | 河北省农林科学院遗传生理研究所 | Growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands |
| CN108925586A (en) * | 2018-08-24 | 2018-12-04 | 成都特普生物科技股份有限公司 | A kind of antimicrobial composition preparation improving plant pest management effect |
Non-Patent Citations (3)
| Title |
|---|
| 张坤鹏 等: "果树根际植物激素产生菌对果树的促生作用", 《落叶果树》 * |
| 李晴晴 等: "根际微生物组介导的解淀粉芽孢杆菌FH-1对水稻的促生机制", 《微生物学报》 * |
| 王欢 等: "有机磷降解菌的筛选及其促生特性", 《微生物学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mehmood et al. | In vitro production of IAA by endophytic fungus Aspergillus awamori and its growth promoting activities in Zea mays | |
| Gopalakrishnan et al. | Plant growth-promoting activities of Streptomyces spp. in sorghum and rice | |
| Ashrafuzzaman et al. | Efficiency of plant growth-promoting rhizobacteria (PGPR) for the enhancement of rice growth | |
| Zhao et al. | Inoculation of soil by Bacillus subtilis Y-IVI improves plant growth and colonization of the rhizosphere and interior tissues of muskmelon (Cucumis melo L.) | |
| Hamdali et al. | Rock phosphate-solubilizing Actinomycetes: screening for plant growth-promoting activities | |
| AU2014203771B2 (en) | Methods and compositions for increasing the amounts of phosphorus available for plant uptake from soils | |
| CN101947545B (en) | A method for bioremediation of organophosphorus pesticide-contaminated soil | |
| CN112625970B (en) | Burkholderia cepacia JT79 and application thereof | |
| Mehmood et al. | IAA producing endopytic fungus Fusariun oxysporum wlw colonize maize roots and promoted maize growth under hydroponic condition | |
| Huang et al. | Effect of organic amendments on Verticillium wilt of cotton | |
| CN109679884B (en) | A high-efficiency maize growth-promoting bacterium that can reduce nitrogen and phosphorus fertilizer application and its application | |
| CN105670963A (en) | Compound microbial fertilizer and preparation method thereof | |
| El-Shabrawy et al. | Controlling maize late-wilt and enhancing plant salinity tolerance by some rhizobacterial strains | |
| CN107094557A (en) | A kind of method for reducing rice grain heavy metal cadmium content | |
| Pushpa et al. | Development of liquid formulation for the dual purpose of crop protection and production | |
| Roy Chowdhury et al. | Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from agricultural field and their potential role on germination and growth of spinach (Spinacia oleracea L.) plants | |
| CN110684683A (en) | A kind of Bacillus amyloliquefaciens, bacterial agent and application | |
| CN108034609A (en) | One plant of series bacillus bacterial strain T1-1 with phosphate solubilization and its application | |
| CN111778183B (en) | Acidophilic nitrogen-producing pseudomonas strain and application thereof | |
| CN100366728C (en) | Bacterial strain for preventing and treating cotton verticillium wilt and preparation method thereof | |
| US20060029576A1 (en) | Biological control of pythium disease in crops | |
| Nambiar | Response of groundnut (Arachis hypogaea L) to Rhizobium inoculation in the field: Problems and prospects | |
| JP4472945B2 (en) | Microorganisms for controlling plant viruses, plant virus control agents comprising the microorganisms, and methods for controlling plant viruses using the microorganisms | |
| Minakshi et al. | Optimization of IAA production and P-solubilization potential in Bacillus subtilis KA (1) 5r isolated from the medicinal herb Aconitum heterophyllum-growing in western Himalaya, India | |
| CN113088461A (en) | Microbial compound inoculant and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210709 |