CN108743432A

CN108743432A - A kind of preparation method of the anti-wrinkle Essence containing ooze extract

Info

Publication number: CN108743432A
Application number: CN201810881013.2A
Authority: CN
Inventors: 单筱慧
Original assignee: Wuhu Hui Yu Commerce Co Ltd
Current assignee: Wuhu Hui Yu Commerce Co Ltd
Priority date: 2018-08-04
Filing date: 2018-08-04
Publication date: 2018-11-06

Abstract

A kind of preparation method of the anti-wrinkle Essence containing ooze extract, belongs to Essence preparing technical field, including：It prepares ooze extract, prepare small molecule collagen polypeptide extracting solution, the preparation of water phase liquid, emulsification, cool down and etc..The present invention is added to as natural additive in cosmetics using ooze extract, has effects that excellent anti-wrinkle is crease-resistant.Collagen and polypeptide can provide energy for skin, adjust internal moisture, promote the synthesis and regulation and control of protein, enzyme, contribute to metabolism, promote cell Proliferation, differentiation, can repair cell, crease-resistant, anti-oxidant and anti-aging.

Description

A kind of preparation method of the anti-wrinkle Essence containing ooze extract

Technical field

The present invention relates to Essence preparing technical field, more particularly to a kind of anti-wrinkle Essence containing ooze extract Preparation method.

Background technology

Essence is the superfine product in skin care item, and ingredient is exquisite, effect is powerful, significant effect, remains what it possessed It is noble and mysterious, it is suitable for any skin or inclined oily skin, preferably puts in the vial, take at any time.Essence is used for toner Later, before face cream.Commonly there is the problems such as poor permeability, be not easy to absorb, and single effect in current Essence, functional It is not strong.

Invention content

It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of anti-wrinkle Essence containing ooze extract Preparation method, there is good penetrability, easily absorb, the effect of anti-wrinkle.

The present invention uses following technical scheme：

A kind of preparation method of the anti-wrinkle Essence containing ooze extract, includes the following steps：

Step 1：The ooze acquired using seabeach is no more than 40 DEG C of drying, sorts out impurity, at room temperature with 10-15 times of sea The ethanol solution of shale amount extracts the ooze 24 hours, and the mass concentration of the ethanol solution is 30%-90%, then high speed from The heart, 3000 revs/min -4000 revs/min of rotating speed, centrifugation time are -25 minutes 15 minutes, and obtained supernatant is ooze alcohol extracting Ooze alcohol extract is carried out decompression rotary evaporation and removes solvent, obtains ooze extract by liquid；

Step 2：It first uses the ethyl alcohol of mass concentration 60-80% to impregnate fish-skin bone 30 seconds, then is carefully cleaned with clear water, removed Fats impurity cuts disrupting tissue, water wash 3 times with knife later, then presses fish-skin bone with water quality than 1:5 ratio impregnates fish-skin Bone adjusts pH value to 6.9-8.5, is heated to 100 degree, keeps the temperature 1 hour, be cooled to water temperature 40-50 degree, use 150- later 300 mesh filter 2-3 times, obtain macromolecular collagen protein extracting solution, and wherein collagen content is 0.05-1g/ml；

Step 3：Macromolecular collagen protein extracting solution is digested with biological enzyme, biological enzyme used is stomach cardia Enzyme, a concentration of 0.0001-500mg/ml digest within 24 hours, are put into 90 DEG C of water-baths and be cooled to room temperature after destroying enzyme activity, from Heart processing, 4500-10000rpm centrifuge 30-10min, finally take supernatant, obtain small molecule collagen polypeptide extracting solution, Middle collagen polypeptide content is 0.05-1g/ml；

Step 4：It weighs in deionized water to water phase pot, sequentially adds the measured EDETATE SODIUM of accurate scale, hyaluronic acid Sodium, carbomer, and heating mixes while stirring, is sufficiently stirred and is uniformly dissolved under temperature 60 C, sequentially adds propylene glycol, 1,3- Butanediol, methyl hydroxybenzoate, allantoin, temperature 60 C keep the temperature 30 minutes；

Step 5：Material in water phase pot is pumped into vacuum emulsifying pot, ooze extraction is sequentially added under stirring Object, small molecule collagen polypeptide extracting solution, propolis extract, protease, aminopeptidase, camomile extract, licorice, ferment Female polypeptide, tip edge bladder-wrack extract, oligopeptides -20 stir 30 minutes, are sufficiently mixed uniformly；

Step 6：It cools, when temperature drops to 45 DEG C, Cremophor RH40, blumea oil, octanoic acid is added Glyceride, and stir；

Step 7：Continue to cool down, when temperature drops to 35 DEG C, aminomethyl propanol is added, and stir, then cold But to room temperature to get Essence.

Preferably, the propolis extract is nanometer supercritical bee-glue.

It is further preferred that the nanometer supercritical bee-glue is the nanometer being process by Phospholipids nano-encapsulated technology Supercritical bee-glue.

Preferably, the licorice is prepared using following methods：5g licorice medicinal materials are crushed, sieving, obtains powder The NaOH aqueous solutions of a concentration of 0.2mol/L are added in body, are 1 by solid-to-liquid ratio:10 ratios mix, the hot dipping extraction 2h at 80 DEG C, It is repeated 2 times, filters, it is 3% hydrochloric acid tune pH value to 1-2.5 that mass concentration is added in filtrate, is centrifuged under the rotating speed of 4000r/min Separation, goes supernatant to obtain sediment, as licorice.

Preferably, the camomile extract is prepared using following methods：5g chamomiles are crushed, sieving, obtains powder The NaOH aqueous solutions of a concentration of 0.2mol/L are added in body, are 1 by solid-to-liquid ratio:10 ratios mix, the hot dipping extraction 2h at 80 DEG C, It is repeated 2 times, filters, it is 3% hydrochloric acid tune pH value to 1-2.5 that mass concentration is added in filtrate, is centrifuged under the rotating speed of 4000r/min Separation, goes supernatant to obtain sediment, as camomile extract.

Preferably, the tip edge bladder-wrack extract is prepared using following methods：Tip edge bladder-wrack is washed, is put It sets and is air-dried at aeration-drying, the tip edge bladder-wrack after air-drying is placed in micro-wave oven, moderate heat dries 4min, after drying Tip edge bladder-wrack be put into pulverizer and crush, the tip edge bladder-wrack after crushing is crossed into 60 mesh sieve, tip edge bladder-wrack powder is obtained, weighs Tip edge bladder-wrack powder 10g, deionized water 100g, are added to the container, and extraction 100min are decocted at 90 DEG C, by the substance in container It all takes out, is cooled to room temperature, filtered with cryogenic freezing centrifuge, rotating speed 3000r/min, centrifugation time 5min, in collection Clear liquid obtains tip edge bladder-wrack extract after filtering.

It is further preferred that described is filtered into three layers of filtered through gauze.

It is further preferred that being filtered using the membrane filter in 0.2 μm of aperture after three layers of filtered through gauze.

Preferably, the grinding and sieving was 40 mesh sieve.

The beneficial effects of the present invention are：

1) ooze extract is added to as natural additive in cosmetics, has effects that excellent anti-wrinkle is crease-resistant.

2) collagen can provide energy with polypeptide for skin, adjust internal moisture, promote the synthesis of protein, enzyme With regulation and control, contribute to metabolism, promote cell Proliferation, differentiation, can repair cell, it is crease-resistant, anti-oxidant and slow down aging.

3) the effect of Radix Glycyrrhizae has clearing heat and detoxicating, antibacterial anti-inflammatory is suitble to various skin, is particularly suitable for sensibility skin, ocean Camomile have disinfecting, antimicrobial, releive sensitive skin the effect of.

4) present invention uses nanometer supercritical bee-glue, it is easy to which infiltration and absorption successfully solve and other ingredients Compatibility issues, nanometer supercritical bee-glue have the function of that strength is anti-oxidant, inhibit tyrosinase activity, and whitening spot-removing effect is aobvious It writes.

5) tip edge bladder-wrack can provide the natural nutrient needed for skin rapid regeneration and cell normal growth, extra-nutrition It is lost in, promotes skin metabolism, increase the ATP synthesis of skin energy element；It protects skin cell mitochondria and maintains mitochondria work( Energy；Promote skin revitalization, skin is helped to reply health and vigor.

Specific implementation mode

With reference to embodiments, the technical solution in the present invention is clearly and completely described.Based in the present invention Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all Belong to the scope of protection of the invention.

A kind of anti-wrinkle Essence containing ooze extract, includes the raw material of following parts by weight：2-4 parts of ooze extractions Object, 2-4 parts of small molecule collagen polypeptide extracting solutions, 2-4 parts of propolis extracts, 0.5-1 parts of protease, 0.5-1 parts of aminopeptidases, 1- 2 parts of camomile extracts, 1-2 parts of licorices, 1-3 parts of yeast polypeptides, 2-4 parts of tip edge bladder-wrack extracts, 1-3 parts of widows Peptide -20,0.5-1 part carbomer, 0.6-2 parts of aminomethyl propanols, 3-6 parts of propylene glycol, 3-6 parts of 1,3 butylene glycols, 0.5-1 parts of PEG- 40 rilanit specials, 0.5-1 part blumea oil, 0.5-1 parts of glycerol caprylates, 0.1-0.2 parts of methyl hydroxybenzoates, 0.1-0.2 parts of urine Bursin, 0.02-0.1 part EDETATE SODIUM, 0.03-0.1 parts of Sodium Hyaluronates, 70-100 parts of deionized waters.

The propolis extract is nanometer supercritical bee-glue.The nanometer supercritical bee-glue is wrapped by Phospholipids nanometer The nanometer supercritical bee-glue that the technology of wrapping up in is process.

The licorice is prepared using following methods：5g licorice medicinal materials are crushed, sieving, obtain powder, is added The NaOH aqueous solutions of a concentration of 0.2mol/L are 1 by solid-to-liquid ratio:10 ratios mix, and hot dipping extraction 2h, is repeated 2 times at 80 DEG C, Filtering, it is 3% hydrochloric acid tune pH value to 1-2.5 that mass concentration is added in filtrate, centrifuges, goes under the rotating speed of 4000r/min Supernatant obtains sediment, as licorice.

The grinding and sieving was 40 mesh sieve.

The camomile extract is prepared using following methods：5g chamomiles are crushed, sieving, obtain powder, is added The NaOH aqueous solutions of a concentration of 0.2mol/L are 1 by solid-to-liquid ratio:10 ratios mix, and hot dipping extraction 2h, is repeated 2 times at 80 DEG C, Filtering, it is 3% hydrochloric acid tune pH value to 1-2.5 that mass concentration is added in filtrate, centrifuges, goes under the rotating speed of 4000r/min Supernatant obtains sediment, as camomile extract.

The grinding and sieving was 40 mesh sieve.

The tip edge bladder-wrack extract is prepared using following methods：Tip edge bladder-wrack is washed, is placed on logical It air-dries dry place to be air-dried, the tip edge bladder-wrack after air-drying is placed in micro-wave oven, moderate heat dries 4min, by the tip edge after drying Bladder-wrack is put into pulverizer and crushes, and the tip edge bladder-wrack after crushing is crossed 60 mesh sieve, obtains tip edge bladder-wrack powder, weighs tip edge ink Angle algae powder 10g, deionized water 100g, are added to the container, and extraction 100min is decocted at 90 DEG C, the substance in container is all taken Go out, be cooled to room temperature, filtered with cryogenic freezing centrifuge, rotating speed 3000r/min, centrifugation time 5min, collects supernatant, mistake After filter tip edge bladder-wrack extract, it is described to be filtered into three layers of filtered through gauze, using 0.2 after described three layers of filtered through gauze The membrane filter filtering in μm aperture.

The present invention is exemplarily described above, it is clear that present invention specific implementation is not subject to the restrictions described above, As long as using the improvement of the various unsubstantialities of inventive concept and technical scheme of the present invention progress, or not improved this is sent out Bright design and technical solution directly applies to other occasions, within protection scope of the present invention.The protection of the present invention Range should be determined by the scope of protection defined in the claims.

Claims

1. a kind of preparation method of the anti-wrinkle Essence containing ooze extract, which is characterized in that include the following steps：

Step 1：The ooze acquired using seabeach is no more than 40 DEG C of drying, impurity is sorted out, at room temperature with 10-15 times of ooze matter The ethanol solution of amount extracts the ooze 24 hours, and the mass concentration of the ethanol solution is 30%-90%, then high speed centrifugation, 3000 revs/min -4000 revs/min of rotating speed, centrifugation time are -25 minutes 15 minutes, and obtained supernatant is ooze alcohol extract, Ooze alcohol extract is subjected to decompression rotary evaporation and removes solvent, obtains ooze extract；

Step 2：It first uses the ethyl alcohol of mass concentration 60-80% to impregnate fish-skin bone 30 seconds, then is carefully cleaned with clear water, removal fat Class impurity cuts disrupting tissue, water wash 3 times with knife later, then presses fish-skin bone with water quality than 1:5 ratio impregnates fish-skin bone, PH value is adjusted to 6.9-8.5,100 degree is heated to, keeps the temperature 1 hour, be cooled to water temperature 40-50 degree, use 150-300 later Mesh filters 2-3 times, obtains macromolecular collagen protein extracting solution, and wherein collagen content is 0.05-1g/ml；

Step 3：Macromolecular collagen protein extracting solution is digested with biological enzyme, biological enzyme used is pepsin, dense Degree is 0.0001-500mg/ml, digest within 24 hours, puts into 90 DEG C of water-baths and is cooled to room temperature after destroying enzyme activity, at centrifugation Reason, 4500-10000rpm centrifuge 30-10min, finally take supernatant, obtain small molecule collagen polypeptide extracting solution, wherein glue Former content of peptides is 0.05-1g/ml；

Step 4：Weigh in deionized water to water phase pot, sequentially add the measured EDETATE SODIUM of accurate scale, Sodium Hyaluronate, Carbomer, and heating mixes while stirring, is sufficiently stirred and is uniformly dissolved under temperature 60 C, sequentially adds propylene glycol, 1,3- fourths Glycol, methyl hydroxybenzoate, allantoin, temperature 60 C keep the temperature 30 minutes；

Step 5：It material will be pumped into vacuum emulsifying pot in water phase pot, ooze extract, small is sequentially added under stirring Molecule collagen polypeptide extracting solution, propolis extract, protease, aminopeptidase, camomile extract, licorice, yeast are more Peptide, tip edge bladder-wrack extract, oligopeptides -20 stir 30 minutes, are sufficiently mixed uniformly；

Step 6：It cools, when temperature drops to 45 DEG C, Cremophor RH40, blumea oil, sad glycerine is added Ester, and stir；

Step 7：Continue to cool down, when temperature drops to 35 DEG C, aminomethyl propanol is added, and stir, is cooled to Room temperature is to get Essence.

2. a kind of preparation method of anti-wrinkle Essence containing ooze extract according to claim 1, it is characterised in that： The propolis extract is nanometer supercritical bee-glue.

3. a kind of preparation method of anti-wrinkle Essence containing ooze extract according to claim 2, it is characterised in that： The nanometer supercritical bee-glue is the nanometer supercritical bee-glue being process by Phospholipids nano-encapsulated technology.

4. a kind of preparation method of anti-wrinkle Essence containing ooze extract according to claim 1, which is characterized in that The licorice is prepared using following methods：5g licorice medicinal materials are crushed, sieving, obtain powder, is added a concentration of The NaOH aqueous solutions of 0.2mol/L are 1 by solid-to-liquid ratio:10 ratios mix, and hot dipping extraction 2h, is repeated 2 times at 80 DEG C, filters, In filtrate be added mass concentration be 3% hydrochloric acid tune pH value to 1-2.5, centrifuged under the rotating speed of 4000r/min, remove supernatant Obtain sediment, as licorice.

5. a kind of preparation method of anti-wrinkle Essence containing ooze extract according to claim 1, which is characterized in that The camomile extract is prepared using following methods：5g chamomiles are crushed, sieving, obtain powder, is added a concentration of The NaOH aqueous solutions of 0.2mol/L are 1 by solid-to-liquid ratio:10 ratios mix, and hot dipping extraction 2h, is repeated 2 times at 80 DEG C, filters, In filtrate be added mass concentration be 3% hydrochloric acid tune pH value to 1-2.5, centrifuged under the rotating speed of 4000r/min, remove supernatant Obtain sediment, as camomile extract.

6. a kind of preparation method of anti-wrinkle Essence containing ooze extract according to claim 1, which is characterized in that The tip edge bladder-wrack extract is prepared using following methods：Tip edge bladder-wrack is washed, is placed at aeration-drying It is air-dried, the tip edge bladder-wrack after air-drying is placed in micro-wave oven, moderate heat dries 4min, and the tip edge bladder-wrack after drying is put Enter in pulverizer and crush, the tip edge bladder-wrack after crushing is crossed into 60 mesh sieve, tip edge bladder-wrack powder is obtained, weighs tip edge bladder-wrack powder 10g, deionized water 100g, are added to the container, and extraction 100min is decocted at 90 DEG C, the substance in container are all taken out, drop It warms to room temperature, is filtered with cryogenic freezing centrifuge, rotating speed 3000r/min, centrifugation time 5min, supernatant is collected, after filtering Obtain tip edge bladder-wrack extract.

7. a kind of preparation method of anti-wrinkle Essence containing ooze extract according to claim 6, it is characterised in that： Described is filtered into three layers of filtered through gauze.

8. a kind of preparation method of anti-wrinkle Essence containing ooze extract according to claim 7, it is characterised in that： It is filtered using the membrane filter in 0.2 μm of aperture after three layers of filtered through gauze.

9. a kind of preparation method of anti-wrinkle Essence containing ooze extract according to claim 4 or 5, feature exist In：The grinding and sieving was 40 mesh sieve.