CN107897210A - A kind of bactericidal composition containing fenoxanil and bacillus subtilis - Google Patents
A kind of bactericidal composition containing fenoxanil and bacillus subtilis Download PDFInfo
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- CN107897210A CN107897210A CN201711230139.5A CN201711230139A CN107897210A CN 107897210 A CN107897210 A CN 107897210A CN 201711230139 A CN201711230139 A CN 201711230139A CN 107897210 A CN107897210 A CN 107897210A
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- bacillus subtilis
- fenoxanil
- bactericidal composition
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- rice blast
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
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- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of bactericidal composition containing fenoxanil and bacillus subtilis, active ingredient is made of fenoxanil and bacillus subtilis, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, the weight ratio of fenoxanil and bacillus subtilis is 5 80:1.The bactericidal composition of the present invention is used for preventing rice blast, it is synergistic significantly, can greatly improve drug effect, reduces pesticide cost, is conducive to delay Disease Resistance to develop.
Description
Technical field
The present invention relates to technical field of pesticide, more particularly to a kind of Bactericidal composite containing fenoxanil and bacillus subtilis
Thing.
Background technology
Fenoxanil, English are general entitled：Fenoxanil, belongs to benzene oxanamide series bactericidal agent, its mechanism of action is melanin
Biosynthesis inhibitor, mainly suppresses the activity of pillar spore ketone dehydrogenase, is formed so as to suppress Pyricularia oryzae melanin, mainly
For preventing the rice blast of rice.With good absorbability and remarkable wholesomeness, also have very to the blade being newly unfolded after dispenser
Good effect, dispenser remains to suppress spore on scab for 40 days and comes off and disperse, so as to avoid superinfection.
Bacillus subtilis, English name：Bacillus subtilis, are a kind of microbial bactericides, by effectively into
Divide the antibacterial material and site competition, the mode of action of induction of resistance of producing bacillus subtilis life, kill and control pathogen, from
And achieve the purpose that controlling disease.
In agricultural production, single medicament long term frequent uses, and germ easily develops immunity to drugs.Fenoxanil prolonged application
In the prevention of rice blast, the reduction of its drug effect, resistance to the action of a drug increase are reflected in some areas.By the mixed pesticide of heterogeneity,
It is to prevent the very common method of resistance disease.Screened by formulation of pesticide, synergy is significantly formulated, and can effectively improve actual prevention
Effect, reduces dosage, delays Disease Resistance to develop, be the important means of the disease comprehensive regulation.
Studied by inventor, find fenoxanil and bacillus subtilis with certain weight ratio mixture being applied to
The prevention of rice blast has good synergistic effect.
At present, the research for fenoxanil and bacillus subtilis mixture being applied to rice blast there are no relevant report.
The content of the invention
It is an object of the present invention to provide a kind of bactericidal composition containing fenoxanil and bacillus subtilis, for preventing
Rice blast is controlled, it is synergistic significantly, can greatly improve drug effect, reduces pesticide cost, is conducive to delay Disease Resistance to send out
Exhibition.
The present invention adopts the technical scheme that：A kind of bactericidal composition containing fenoxanil and bacillus subtilis, effectively
Component is made of fenoxanil and bacillus subtilis, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be
The weight ratio of 100% active compound, fenoxanil and bacillus subtilis is 5-80:1, it is preferred that fenoxanil and bacillus subtilis
The weight ratio of bacterium is 20:1.
The bactericidal composition, the percentage by weight of fenoxanil are 5.0%-20.0%, the weight of bacillus subtilis
Amount percentage is 0.1-1.0%, i.e., the content of bacillus subtilis in the composition for 1,000,000,000 spores/gram~10,000,000,000 spores/
Gram.
The bactericidal composition can add corresponding auxiliary element, make according to well known to a person skilled in the art method
It is standby into agriculturally acceptable any formulation, it is relatively good for suspending agent, wettable powder etc..
The auxiliary element that the bactericidal composition is prepared into pesticidal preparations addition includes filler, solvent, dispersant, wetting
Agent, emulsifying agent, antifreeze, defoamer, thickener, stabilizer or it is other be beneficial to active ingredient in the formulation stablize and drug effect hair
The material waved.
The present invention has the following advantages and significant technique effect:(1) mechanism of action of two active ingredient of the invention is not
Together, the two mutual mixture germ is not likely to produce resistance.(2) indoor biometrics the result shows that, fenoxanil and bacillus subtilis are pressed
Certain ratio mixture acts on rice blast with notable synergistic, can greatly improve drug effect, advantageously reduce applying for each single dose
Dosage, reduces pesticide cost, is conducive to extend the life cycle of fenoxanil.
Embodiment
Technical scheme and technique effect are described further below by way of specific embodiment, institute of the present invention
The percentage stated is weight percentage.
First, indoor biometrics are tested
Embodiment 1:The Toxicity Determination of fenoxanil, bacillus subtilis and mixture to rice blast
After prerun determines the effective inhibition concentration scope of each medicament, by active constituent content, gradient sets 5 to each medicament respectively
A dosage processing, if clear water compares.Reference《Farm-chemical indoor determination test rule fungicide》Carry out, using mycelial growth speed
Rate method measures virulence of the medicament to rice blast.Colony diameter is measured with crossing method after 72h, calculates the net growth of each processing
Amount, mycelial growth inhibition rate.
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is converted into numerical value (x), with minimum
Square law tries to achieve virulence regression equation (y=a+bx), and thus calculates the EC of every kind of medicament50Value, medicine is calculated according to the abundant methods of Sun Yun
The toxicity index and co-toxicity coefficient (CTC) of agent.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100
Percentage composition+B the medicament toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100
When CTC≤80, then composition shows as antagonism, and as 80 ＜ CTC ＜ 120, then composition shows as being added and makees
With when CTC >=120, then composition shows as synergistic effect.
Statistical result is shown in Table 1.
The Toxicity Determination of 1 fenoxanil of table, bacillus subtilis and its mixture to rice blast
| Reagent agent | Proportioning | EC50(mg/L) | ATI | TTI | Co-toxicity coefficient (CTC) |
| Fenoxanil (A) | / | 2.41 | 59.42 | / | / |
| Bacillus subtilis (B) | / | 1.43 | 100.00 | / | / |
| A：B | 5:1 | 1.68 | 85.55 | 66.18 | 129.3 |
| A：B | 10:1 | 1.56 | 92.19 | 63.11 | 146.1 |
| A：B | 15:1 | 1.33 | 107.74 | 61.95 | 173.9 |
| A：B | 20:1 | 1.12 | 128.01 | 61.35 | 208.7 |
| A：B | 30:1 | 1.39 | 103.09 | 60.73 | 169.8 |
| A：B | 40:1 | 1.62 | 88.55 | 60.41 | 146.6 |
| A：B | 50:1 | 1.91 | 75.13 | 60.21 | 124.8 |
| A：B | 80:1 | 1.73 | 82.76 | 59.92 | 138.1 |
| A：B | 1:2 | 1.43 | 100.02 | 86.47 | 115.7 |
| A：B | 1:20 | 1.90 | 75.36 | 98.07 | 76.8 |
Measurement result shows, when the weight ratio of fenoxanil and bacillus subtilis is 5-80:When 1, co-toxicity coefficient (CTC)
120 are all higher than, the wherein weight ratio of fenoxanil and bacillus subtilis is 20:When 1, co-toxicity coefficient (CTC) is maximum, and synergy is made
With significantly, there is good application value in field trials.
2nd, embodiment is prepared
Embodiment 1：10% fenoxanil 5,000,000,000 CFU/ grams bacillus subtilis suspended dose
Formula：It is 20 according to the weight ratio of fenoxanil and bacillus subtilis:1, fenoxanil 10%, withered is weighed according to quantity
Careless bacillus 0.5%, alkyl naphthyl sulfonate 3.5% (wetting agent), polycarboxylate 3.2% (dispersant), xanthans 0.2%
(thickener), ethylene glycol 2% (antifreezing agent), 0.2% silicone oil (defoamer), deionized water are mended to 100%.
Embodiment 2：5% fenoxanil 10,000,000,000 CFU/ grams bacillus subtilis suspended dose
Formula：It is 5 according to the weight ratio of fenoxanil and bacillus subtilis:1, fenoxanil 5.0%, withered is weighed according to quantity
Careless bacillus 1.0%, alkyl benzene sulfonate calcium salt 2.5% (wetting agent), polycarboxylate 5% (dispersant), xanthans 0.5%
(thickener), propane diols 2.5% (antifreezing agent), silicone oil 0.3% (defoamer), deionized water are mended to 100%.
Embodiment 3：15% fenoxanil 2,000,000,000 CFU/ grams bacillus subtilis suspended dose
Formula：It is 75 according to the weight ratio of fenoxanil and bacillus subtilis:1, fenoxanil 15%, withered is weighed according to quantity
Careless bacillus 0.2%, alkyl naphthyl sulfonate 4.5% (wetting agent), polycarboxylate 5% (dispersant), xanthans 0.5%
(thickener), polyethylene glycol 2.5% (antifreezing agent), silicone oil 0.2% (defoamer), deionized water are mended to 100%.
Above-described embodiment 1-3 is prepared in accordance with the following methods：By recipe requirements, the component is uniformly mixed, by grinding
The composition of the bacillus subtilis containing fenoxanil is made in mill and high speed shear, and formulation is suspending agent.
Embodiment 4：5.0% fenoxanil, 1,000,000,000 CFU/ grams of bacillus subtilis wettable powder.
Formula：It is 50 according to the weight ratio of fenoxanil and bacillus subtilis:1, weigh according to quantity fenoxanil 5.0%,
Bacillus subtilis 0.1%, alkyl benzene sulfonate calcium salt 3.5% (dispersant), lignosulfonates 5% (wetting agent), white carbon
10% (filler), concave convex rod (filler) complement to 100%.
Embodiment 5：20% fenoxanil, 10,000,000,000 CFU/ grams of bacillus subtilis wettable powder.
Formula：It is 20 according to the weight ratio of fenoxanil and bacillus subtilis:1, fenoxanil 20%, withered is weighed according to quantity
Careless bacillus 1.0%, polycarboxylate 5.0% (dispersant), lauryl sodium sulfate 4.5% (wetting agent), white carbon 10%
(filler), kaolin (filler) complement to 100%.
Above-described embodiment 4-5 is prepared in accordance with the following methods：By recipe requirements, the component is uniformly mixed, through air-flow
Pulverizer crushes, and can be prepared by the wettable powder of the bacillus subtilis containing fenoxanil.
3rd, field trial embodiment
Embodiment 1:Prepare the field trial of the preparation prevention rice blast of the gained of embodiment 1
Experiment is with reference to GB/T 17980.19-2000 pesticide field efficacy medicine tests criterion (one) bactericidal agent for preventing and treating rice leaf portion
Disease.
Test process：To prepare 1 gained preparation of embodiment, (10% fenoxanil, 5,000,000,000 CFU/ grams of bacillus subtilis is hanged
Floating agent) it is test group 1#, dosage is 45g/ mus；Using 15% fenoxanil suspending agent as control group 1#, dosage is 45g/ mus；
With 20,000,000,000 CFU/ grams bacillus subtilis suspended dose for control group 2#, dosage be 150g/ mus；One blank control is set at the same time
(CK), plot area is 60 ㎡, 4 repetitions.
Experiment and investigation method：In rice plant of tillering stage, rice blast early stage dispenser, dispenser 1 time again in 7 days after medicine, altogether
Twice, each cell is watered even spraying by the water consumption for 45 kgs/acre, using rice leaf moistening as standard for medication.Respectively at
14d carries out disease index investigation after 7d, last medicine before medicine, after last medicine, calculates preventive effect.
Result of the test：7 days after last dispenser, the prevention effect of test group 1# is 95.3%, the prevention effect of control group 1#
For 82.4%, the prevention effect of control group 2# is 73.6%, and the preventive effect of test group 1# is more excellent than control group 1#, control group 2# respectively
More than 10%；14 days after last dispenser, the prevention effect of test group 1# is 92.7%, and the prevention effect of control group 1# is
The prevention effect of 76.4%, control group 2# are 72.5%, and the preventive effect of test group 1# is more excellent than control group 1# and control group 2# respectively
More than 15%.
This explanation, bactericidal composition of the invention, with fenoxanil and bacillus subtilis rational mixture, can effectively prevent
Rice blast is controlled, while synergy is notable, can reduce the amount of application of each single dose, advantageously reduce dispenser cost, be conducive to disease
Resistance management.Test each cell rice in section without poisoning to occur, illustrate the composition of the present invention to rice safety.
Claims (4)
1. a kind of bactericidal composition containing fenoxanil and bacillus subtilis, it is characterised in that active ingredient is by fenoxanil
Formed with bacillus subtilis, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, rice blast
The weight ratio of acid amides and bacillus subtilis is 5-80:1.
2. bactericidal composition according to claim 1, it is characterised in that the weight ratio of fenoxanil and bacillus subtilis
For 20:1.
3. bactericidal composition according to claim 1 or 2, it is characterised in that the percentage by weight of fenoxanil is
5.0%-20.0%, the percentage by weight of bacillus subtilis is 0.1-1.0%.
4. according to the bactericidal composition described in claim 1-3 any one, it is characterised in that the bactericidal composition is applicable in
In prevention rice blast.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711230139.5A CN107897210A (en) | 2017-11-29 | 2017-11-29 | A kind of bactericidal composition containing fenoxanil and bacillus subtilis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711230139.5A CN107897210A (en) | 2017-11-29 | 2017-11-29 | A kind of bactericidal composition containing fenoxanil and bacillus subtilis |
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| CN107897210A true CN107897210A (en) | 2018-04-13 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104507319A (en) * | 2012-05-30 | 2015-04-08 | 拜尔农作物科学股份公司 | Composition comprising a biological control agent and a fungicide selected from inhibitors of the lipid membrane synthesis, the melanine biosynthesis, the nucleic acid synthesis or the signal transduction |
| CN104719344A (en) * | 2009-03-26 | 2015-06-24 | 拜尔作物科学有限公司 | Use of synthetic and biological fungicides in combination for controlling harmful fungi |
| CN105142405A (en) * | 2013-03-20 | 2015-12-09 | 巴斯夫公司 | Synergistic compositions comprising a bacillus subtilis strain and a pesticide |
| CN105961379A (en) * | 2016-05-04 | 2016-09-28 | 南京高正农用化工有限公司 | Large composition floating granule for preventing and controlling sheath blight and rice blast of rice, and preparation method thereof |
-
2017
- 2017-11-29 CN CN201711230139.5A patent/CN107897210A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104719344A (en) * | 2009-03-26 | 2015-06-24 | 拜尔作物科学有限公司 | Use of synthetic and biological fungicides in combination for controlling harmful fungi |
| CN104507319A (en) * | 2012-05-30 | 2015-04-08 | 拜尔农作物科学股份公司 | Composition comprising a biological control agent and a fungicide selected from inhibitors of the lipid membrane synthesis, the melanine biosynthesis, the nucleic acid synthesis or the signal transduction |
| CN105142405A (en) * | 2013-03-20 | 2015-12-09 | 巴斯夫公司 | Synergistic compositions comprising a bacillus subtilis strain and a pesticide |
| CN105961379A (en) * | 2016-05-04 | 2016-09-28 | 南京高正农用化工有限公司 | Large composition floating granule for preventing and controlling sheath blight and rice blast of rice, and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李晶等: "生防枯草芽孢杆菌的研究进展", 《安徽农业科学》 * |
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Application publication date: 20180413 |