CN107410291A - A kind of African Chrysanthemum cut flower vase keeps liquid and preparation method thereof - Google Patents
A kind of African Chrysanthemum cut flower vase keeps liquid and preparation method thereof Download PDFInfo
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- CN107410291A CN107410291A CN201710807192.0A CN201710807192A CN107410291A CN 107410291 A CN107410291 A CN 107410291A CN 201710807192 A CN201710807192 A CN 201710807192A CN 107410291 A CN107410291 A CN 107410291A
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- african chrysanthemum
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- cut flower
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
- A01N3/02—Keeping cut flowers fresh chemically
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of African Chrysanthemum cut flower vase to keep liquid and preparation method thereof, inserts for this bottle and keeps liquid to be mainly made up of 8 oxyquinolines and 60g/L sucrose that mass concentration is 150mg/L；Inserting for this bottle keeps preparing for liquid quick and easy, without using the operation such as heat, pressurize；Keep liquid low raw-material cost, wherein 150mg/L 8 oxyquinolines are sufficient to effectively suppress the bacterium mainly grown during African Chrysanthemum bottle is inserted, cost can be substantially reduced and reduce injury of the bacteriostatic agent to African Chrysanthemum Fresh Cutting flower by commonly using Mlc compared to it, and the more targeted technical problem such as bacteria breed and the easy muddiness of liquid for immersing in bottle during solving African Chrysanthemum cut flower vase.
Description
Technical field
The invention belongs to Fresh Cutting flower technical field of preservation of fresh, and in particular to a kind of African Chrysanthemum cut flower vase keeps liquid and its system
Preparation Method.
Background technology
Fresh Cutting flower post-harvest fresh-keeping is in widespread attention at home and abroad.African Chrysanthemum is one of five big Fresh Cutting flower of the world, its fresh-cut
Flower breed bacteria easy in bottle inserts preservation process causes bottle to insert solutions turbid, influences bottle and inserts appreciation effect, further can also shadow
Fresh Cutting flower flower water absorbing capacity is rung, causes flower to wither, terminates the cut flower vase life-span too early.
Solution haze is inserted in order to reduce bottle, effectively extends the African Chrysanthemum cut flower vase life-span, improves cut flower vase and see
Quality is appreciated, the fresh-keeping formula of liquid for commonly using argentiferous on the market carrys out the slotting culture African Chrysanthemum Fresh Cutting flower of bottle, and not only cost is high, its discarded bottle
Slotting solution is also easy to pollute environment, and hidden danger is brought to user's personal safety.Research shows both at home and abroad, 8-hydroxyquinoline and its sulphur
The broad spectrum activity that hydrochlorate, citrate can be used in practice is antibacterial.Because 8-hydroxyquinoline is not readily dissolved in water, in the prior art often
8-hydroxyquinoline is replaced using 8-hydroxyquinoline sulfate soluble in water, 8-hydroxyquinoline citrate, for Fresh Cutting flower
Fresh-keeping, its concentration is more than or equal to 200mg/L；Or helped in fresh-keeping liquid preparation process using technologies such as heating, pressurizations
8-hydroxyquinoline dissolves.The former cost of material is higher, and the latter's operation is relatively complicated.In addition, that is reported in the prior art is fresh-keeping
The clarity of liquid is not high, and aesthetics is bad, also have impact on the market value of fresh-keeping liquid to a certain extent.
Present invention aims at solving the defects of above-mentioned prior art is present, a kind of single-minded inserted for African Chrysanthemum bottle of research and development is seen
The main bacteria species occurred in reward, it is economical and practical, prepare it is simple, as clear as crystal and can the African Chrysanthemum fresh-cut that uses of safety and effectivity
Vase inserts retention agent, has higher application value and good market prospects.
The content of the invention
It is an object of the invention to provide a kind of African Chrysanthemum Fresh Cutting flower holding liquid and its preparation side during viewing and admiring are inserted in bottle
Method, the slotting holding liquid cost of the bottle is relatively low, preparation method is quick and easy, solution is persistently limpid, effect is good and less toxic pollution-free.
In order to solve the above problems, the technical scheme that the present invention takes is：
A kind of African Chrysanthemum cut flower vase keeps liquid, mainly the 8-hydroxyquinoline and 60g/L by mass concentration for 150mg/L
Sucrose composition.
Its preparation method is simple and quick, without pressurization or heating operation, concretely comprises the following steps：
(1) 0.15g 8-hydroxyquinoline is weighed first, and adds 0.5mL 1mol/L hydrochloric acid solutions by above-mentioned 8- hydroxyls
Quinoline is completely dissolved, and then adds distilled water or running water dilutes constant volume to 1L, obtain solution I；
(2) 60g sucrose is weighed, and is completely dissolved in appropriate solution I, then with solution I again constant volume to 1L, i.e.,
Obtain the African Chrysanthemum cut flower vase and keep liquid.
In addition, the application of the African Chrysanthemum Fresh Cutting flower bacteriostasis, preservation agent is also claimed in the present invention, it is specially：Under water will
African Chrysanthemum Fresh Cutting flower spray tail end is chamfer, and it is 25~30cm to retain spray length, and spray then is inserted directly into the antibacterial guarantor
Bottle inserts culture in fresh dose, and ensures that spray tail end otch below liquid level, during which need not change water.
African Chrysanthemum cut flower vase provided by the invention keeps liquid, compared with conventional fresh cut-flower antistaling agent, has following
Obvious beneficial effect：
(1) relative to argentiferous antistaling agent in the prior art, the African Chrysanthemum cut flower vase in the present invention keeps liquid toxicity low,
More environmentally friendly economy；
(2) relative to the 8-hydroxyquinoline sulfate or 8-hydroxyquinoline citrate used in the prior art, the present invention
In 8-hydroxyquinoline cost of material it is lower, antipathogenic composition usage amount has also obtained further optimal control, and antibacterial specific aim is more
By force；
(3) relative to the dissolving difficulty problem of 8-hydroxyquinoline in the prior art, the bottle in the present invention, which is inserted, keeps liquid to prepare
Cheng Gengjia simplifies, without the cumbersome preparation process such as heat or pressurize.
(4) African Chrysanthemum cut flower vase of the present invention keep liquid mainly solve African Chrysanthemum bottle insert view and admire middle holding liquid it is muddy,
Aesthetics is bad, Vase time is short and bottle inserts solution and changes the problems such as frequent, the discovery of the invention, 150mg/L 8- hydroxyls
The composition of base quinoline and 60g/L sucrose, which coordinates, can effectively solve the problem that the problem of keeping liquid muddiness ornamental flaw, have preferably non-
Continent chrysanthemum bottle inserts appreciation effect, this be the present invention to one of main contributions of prior art, be asking of being not concerned with of existing researcher
One of topic.
Brief description of the drawings
Fig. 1 is that the African Chrysanthemum floral disc of the embodiment of the present invention 2 deploys situation and vase flower arrangement solution dehydration volume diagram.
Embodiment
Technical scheme is further elaborated with reference to embodiment：
Embodiment 1
8-hydroxyquinoline is broad spectrum activity bacteriostatic agent, and obtaining its selectivity by embodiment 1 suppresses the slotting bacterium taste of African Chrysanthemum bottle
Raw minimum concentration.
(1) main bacteria bacterium colony during African Chrysanthemum clear water bottle is inserted is collected by PCA plating methods and isolated and purified.Utilize
Bacterial 16 S rDNA hypervariable region design special primer, expands and is sequenced the 16S rDNA codings for obtaining main bacteria seed by PCR
Sequence；In NCBI BLAST websites aligned sequences homology, determine these strains mostly come from general Pseudomonas, pseudomonas,
Stenotrophomonas and Klebsiella.
(2) main bacteria is inserted by LB fluid nutrient medium concussion and cultivate method culture African Chrysanthemums bottle.Concrete operations are：
The African Chrysanthemum bottle that 10ml LB culture mediums and 30 μ l are respectively put into 150ml conical flasks inserts bacterium, with 50mg/L, 100mg/L,
150mg/L, 200mg/L 8-hydroxyquinoline are bacteriostatic agent, make an addition to shake in bacterium culture medium respectively, with the culture medium without bacteriostatic agent
For control 1, using without bacteriostatic agent without the culture medium that bacterium add for compare 2, rotating speed be 200rpm shaking table on concussion and cultivate.
The OD600 values of each solution are detected during Bacteria Culture the 7th day, to determine effectively to suppress the 8- hydroxyls that African Chrysanthemum bottle inserts bacterial reproduction
Quinoline least concentration.
(3) drawn from the data of table 1,150mg/L 8-hydroxyquinoline is that effective African Chrysanthemum bottle that suppresses inserts period bacterium taste
Raw least concentration.It is compared to the conventional 200~300mg/L Mlcs of 8-hydroxyquinoline and its esters and in the market is normal
Argentiferous Fresh Cutting flower bacteriostasis, preservation agent, 150mg/L 8-hydroxyquinoline can reduce excessive concentrations or poisonous bacteriostatic agent to fresh-cut
The injury that flower spray brings, and reduce and prepare the cost that bottle inserts holding liquid.Therefore, the present invention selects 150mg/L 8- hydroxyl quinolines
Quinoline keeps the main antipathogenic composition in liquid as African Chrysanthemum cut flower vase.
The bacterial liquid nutrient solution of table 1 shakes OD600 value of the bacterium after 7 days
Embodiment 2：
(1) African Chrysanthemum " pigment " kind is selected, chooses no disease and pests harm, bennet is upright, Hua Jing is homogeneous, outer layer ligulate flower is complete
The flowers are in blossom puts the Fresh Cutting flowers of 1-2 wheels for open, internal layer tubulose；African Chrysanthemum spray tail end is chamfer in clear water, retains the long 30cm of spray；
Spray is inserted in the container containing African Chrysanthemum cut flower vase bacteriostasis, preservation agent, ensures spray tail end otch below liquid level.
Using sterile Aquaponic as control (CK), liquid is kept to handle African Chrysanthemum Fresh Cutting flower, every bottle of three cut-flowers, treatment group respectively using five kinds
In triplicate.Solution initial volume of being arranged flowers in each vase is 300ml, and room temperature is maintained at 20~25 DEG C, relative humidity 70~80%,
Well-ventilated, material is not by direct sunlight.
Wherein, solution A is that bottle of the present invention inserts holding liquid：In terms of one liter of water, its principle active component includes：60g sucrose and
150mg 8-hydroxyquinoline；The bottle insert keep liquid preparation method be：0.15g 8-hydroxyquinoline is weighed, it is completely molten
Solution dilutes in 0.5ml 1mol/L hydrochloric acid solutions, and with running water or sterilized water, is settled to 1L, obtains solution I；Weigh
60g sucrose, it is dissolved completely in appropriate solution I, and 1L is settled to solution I, finally give African Chrysanthemum cut flower vase
Keep liquid.The preparation method of other B, C, D, E, F, G, H, I processing solutions is similar with the method for solution A.
Each flower arrangement main component in solution composition see the table below 1：
Table 2 respectively arrange flowers main component in solution composition table
(2) periodically the floral disc deployment diameter of African Chrysanthemum Fresh Cutting flower is measured using ruler, and processing the 10th day it is right
Solution residual volume in each vase measures；For the solution arranged flowers 0 day using the slotting solution of respective bottle as blank control, use is ultraviolet
OD600 numerical value when each flower arrangement solution bottle of spectrophotometer detection is inserted 7 days.
Floral disc expansion situation of African Chrysanthemum " pigment " Fresh Cutting flower during bottle is inserted is shown in vase flower arrangement solution dehydration volume
Figure of description Fig. 1, flower arrangement solution fluid loss intuitively reflect spray water absorbing capacity.
The OD600 values that the limpid effect of solution of the slotting holding liquid of each bottle may be referred to bottle slotting solution when bottle is inserted 7 days are compared,
Concrete outcome is shown in Table 2：
Table 2：Solution O D600 values during African Chrysanthemum cut flower vase 7 days
The result of embodiment 2 shows, the inventive method, i.e., in the premise added with 150mg/L8- oxyquinoline bacteriostatic agents
Under, 60g/L sucrose is added, bottle can be kept to insert the limpid degree of solution liquid to greatest extent, while maintain the suction of African Chrysanthemum Fresh Cutting flower
Outlet capacity, further extends African Chrysanthemum Fresh Cutting flower flower and extends the phase, and extending Fresh Cutting flower relative to control blooms number of days more than 2 days.
Experimental example 3：
(1) African Chrysanthemum " Yunnan Red " kind is selected, chooses no disease and pests harm, bennet is upright, Hua Jing is homogeneous, outer layer ligule is used up
Full-open, internal layer tubulose the flowers are in blossom put 1-2 wheel Fresh Cutting flower；African Chrysanthemum spray tail end is chamfer in clear water, retains spray length
25cm；Spray is inserted in the container containing African Chrysanthemum Fresh Cutting flower bacteriostasis, preservation agent, ensures spray tail end otch below liquid level.
Using sterile Aquaponic as control (CK), inserted using five kinds of bottles and keep liquid to handle African Chrysanthemum Fresh Cutting flower, every bottle of 2 cut-flowers, place respectively
In triplicate, flower arrangement solution initial volume is 300ml to reason group in each vase；Room temperature is maintained at 20~25 DEG C, and relative humidity 70~
80%, well-ventilated, material is not by direct sunlight.Wherein each antistaling agent prescription scheme and the equal reference implementation example 2 of preparation method, this
Place repeats no more.Solution A is that bottle of the present invention inserts holding liquid.
(2) with the curved stem of flower be more than 90 degree, either flower petal occur since tip 1/2 wither or flower 2/3 with
The end that upper petal dehydration was wilted as the cut flower vase life-span, the bottle of statistics African Chrysanthemum " Yunnan Red " each Fresh Cutting flower spray insert day
Number, concrete outcome see the table below 3：
The African Chrysanthemum of table 3 " Yunnan Red " Vase time
The result of embodiment 3 shows that it is fresh that the inventive method is respectively provided with same extension to the Fresh Cutting flower of different African Chrysanthemum kinds
Cutflower preservation effect, it is adapted to a variety of African Chrysanthemum cut flower vase to view and admire use.
In summary, the inventive method has following feature：
1. the main component 8-hydroxyquinoline concentration in the present invention is 150mg/L, cut flower vase solution can be significantly inhibited
Bacterial growth, maintain bottle to insert solution water quality limpid degree, reduce bottle and insert solution and change water frequency；The concentration is to be directed to African Chrysanthemum
Minimum effective Mlc of the slotting main bacteria of bottle；
2. African Chrysanthemum cut flower vase of the present invention keeps preparing without using the operation such as heat, pressurize in liquid preparation process
It is simple and quick, it is easy to use；
3. the combination of the 8-hydroxyquinoline and sugar composition of suitable concentration in the present invention, in different cultivars African Chrysanthemum Fresh Cutting flower
In can obtain preferably applying result, extend African Chrysanthemum cut flower vase view and admire number of days；
It is user with more facilities in practical use 4. preparing raw material cost of the present invention is cheap and environmentally friendly.
It is last it should be noted that：The invention is not limited in any way for above example.Those skilled in the art are come
Say, on the basis of the present invention, some modification and improvement can be made to it.Therefore, it is all without departing from theon the basis of the spirit of the present invention
Any modification or improvement made, are belonged within the scope of protection of present invention.
Claims (3)
1. a kind of African Chrysanthemum cut flower vase keeps the preparation method of liquid, it is characterised in that is with step specifically：
(1) 0.15g 8-hydroxyquinoline is weighed first, and adds 0.5mL 1mol/L hydrochloric acid solutions by above-mentioned 8-hydroxyquinoline
It is completely dissolved, then adds distilled water or running water dilutes constant volume to 1L, obtain solution I；
(2) 60g sucrose is weighed, and is completely dissolved in appropriate solution I, then with solution I again constant volume to 1L, that is, is obtained
The African Chrysanthemum cut flower vase keeps liquid；
The bottle, which is inserted, keeps liquid to be finally made up of 8-hydroxyquinoline and 60g/L sucrose that mass concentration is 150mg/L.
2. the African Chrysanthemum cut flower vase that preparation method is prepared according to claim 1 keeps liquid.
3. the application of African Chrysanthemum Fresh Cutting flower bacteriostasis, preservation agent described in a kind of claim 1-2, it is characterised in that under water by Africa
Chrysanthemum Fresh Cutting flower spray tail end is chamfer, and it is 25~30cm to retain spray length, and spray then is inserted directly into the bacteriostasis, preservation agent
Middle bottle inserts culture, and ensures that spray tail end otch below liquid level, during which need not change water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710807192.0A CN107410291A (en) | 2017-10-20 | 2017-10-20 | A kind of African Chrysanthemum cut flower vase keeps liquid and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710807192.0A CN107410291A (en) | 2017-10-20 | 2017-10-20 | A kind of African Chrysanthemum cut flower vase keeps liquid and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772641A (en) * | 2021-01-25 | 2021-05-11 | 云南省农业科学院花卉研究所 | Dian clove cut flower preservative |
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| CN1150887A (en) * | 1995-11-21 | 1997-06-04 | 广东省珠海市园艺研究所 | Antistaling agent for cut-flowers in bottle |
| CN1602688A (en) * | 2004-08-19 | 2005-04-06 | 中国农业大学 | Gerbera cut flower pretreatment solution and method of use thereof |
| CN1602681A (en) * | 2004-08-19 | 2005-04-06 | 中国农业大学 | Cut vase cutting fluid and how to use it |
| CN1742575A (en) * | 2005-09-26 | 2006-03-08 | 仲恺农业技术学院 | A method for post-harvest storage and preservation of cut flowers of Heliconia aureus |
| CN101292649A (en) * | 2008-06-05 | 2008-10-29 | 河南科技大学 | Preservative for cut flowers of peony and peony and its application method |
| CN101658162A (en) * | 2009-09-18 | 2010-03-03 | 中国林业科学研究院亚热带林业研究所 | Camellia polyodondta cutflower preservative and preparation method thereof |
| CN104472477A (en) * | 2014-12-18 | 2015-04-01 | 李金枝 | Fresh-keeping agent for cut gerbera flowers |
| KR20150096025A (en) * | 2014-02-13 | 2015-08-24 | 순천대학교 산학협력단 | Method for extending vase life of cut flowers |
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2017
- 2017-10-20 CN CN201710807192.0A patent/CN107410291A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150887A (en) * | 1995-11-21 | 1997-06-04 | 广东省珠海市园艺研究所 | Antistaling agent for cut-flowers in bottle |
| CN1602688A (en) * | 2004-08-19 | 2005-04-06 | 中国农业大学 | Gerbera cut flower pretreatment solution and method of use thereof |
| CN1602681A (en) * | 2004-08-19 | 2005-04-06 | 中国农业大学 | Cut vase cutting fluid and how to use it |
| CN1742575A (en) * | 2005-09-26 | 2006-03-08 | 仲恺农业技术学院 | A method for post-harvest storage and preservation of cut flowers of Heliconia aureus |
| CN101292649A (en) * | 2008-06-05 | 2008-10-29 | 河南科技大学 | Preservative for cut flowers of peony and peony and its application method |
| CN101658162A (en) * | 2009-09-18 | 2010-03-03 | 中国林业科学研究院亚热带林业研究所 | Camellia polyodondta cutflower preservative and preparation method thereof |
| KR20150096025A (en) * | 2014-02-13 | 2015-08-24 | 순천대학교 산학협력단 | Method for extending vase life of cut flowers |
| CN104472477A (en) * | 2014-12-18 | 2015-04-01 | 李金枝 | Fresh-keeping agent for cut gerbera flowers |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772641A (en) * | 2021-01-25 | 2021-05-11 | 云南省农业科学院花卉研究所 | Dian clove cut flower preservative |
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Application publication date: 20171201 |