CN106508812A - Rapid and efficient propagation method for neoseiulus barkeri - Google Patents

Abstract

The invention discloses a rapid and high-efficiency breeding method of Neoseius pasteurii, which comprises the following specific steps: a. preparation of artificial feed for feed mite; b. cultivation of feed mite; Seed production and cultivation; d, industrial breeding of Neoseiius pastereii. Through the above-mentioned method, the fast and efficient breeding method of Neoseiius pasteurii of the present invention utilizes two kinds of food mites of different genus, acaroid mites and sweet mite, as the feed mite of common Neoseiusius pastereii Breeding Neoseius mite, can breed hundreds of millions of Neoseius mite, which can be used as natural enemies on various crops, fruits, and vegetables to prevent and control spider mite, thrips, tarsus mite, etc., and the control effect The annual reduction of pesticide use frequency by more than 60% can significantly increase crop yield and quantity, help protect the ecological environment, reduce pesticide residues, and help mass production of pollution-free food, vegetables, fruits and other green foods.

Description

A kind of fast and high-efficiency breeding method of Neoseius pasterii

technical field

The invention relates to a breeding technique for thrips, harmful mites and predatory mites, the natural enemies of nematodes, in particular to an artificial high-efficiency and rapid breeding method for Neoseiusius pasterii, including an artificial feed formula and an industrialized production process.

Background technique

At present, the occurrence of thrips and harmful mites in various crops is very rampant, coupled with the excessive and high-frequency use of pesticides, many thrips and harmful mites have developed strong drug resistance, which has become a very difficult problem in agriculture. For example, the thrips on the cowpea in Hainan is very severe. In severe cases, the yield of cowpea can be reduced by more than 80%. Farmers are forced to use highly toxic and highly toxic pesticides, causing poisonous cowpea to appear, which seriously damages the vital health interests of the people. Some pest mites are also very harmful to tea gardens, strawberries, and citrus, and have developed high resistance to many conventional pesticides. In biological control, the use of beneficial mites (predatory mites) to control thrips and harmful mites is a very promising approach. Although Neoseius pasterei has achieved some results in the traditional control of predatory mite, the traditional method of artificial propagation of Neoseius pasteurii is expensive, and the common method is to use plants to raise harmful mites, and then use harmful mite Rearing Neoseius mite. The production cost is high, the labor cost is large, the cycle is long, and it is impossible to produce a sufficient amount of products.

Neosiusius mite, also known as Amblyseius mite pasteurii, is an important predator mite. The adult mite is about 0.42 mm long, reddish brown, fast-moving and active. The larvae of Neoseius pasteruii are transparent to white, and the eggs are ellipsoid. Neoseius pasterii can eat about 5 thrips per day, and can prey on about 85 thrips in a lifetime. For harmful mites, Panonychus citrus, it can prey on a maximum of 10 per day. Neoseiusius mite is an omnivorous mite that preys on a variety of different species of prey, and can also feed on pollen. Compared with Amblyseius mite cucurbita, Neoseius mite colonizes among plants easily. is a promising predatory mite.

Zhang Yanxuan et al. invented an artificial breeding method for A. cucurbita A. cucurbita (CN02110907.9), in which A. cucurbita A. cucurbita is raised by artificially cultivating acaroid mites (lepidophilus, tyrophagous tyrophagous, and A. gracilis). Zhang Yanxuan et al.'s "production method for predatory mites" application CN200710008808.4 discloses a method for cultivating acaroid mites and then raising Amblyseius pasteurii. Similarly, simple acaroid mites have no effect on the breeding of Neoseiius pasteurii. good. Zhang Yanxuan etc. invented the disclosed breeding method, which is very poor for raising Neoseius pasteurii. The reason is that, on the one hand, Neoseius pasterei is omnivorous, and the palatability and nutrition of the single acaroid mite may not be able to fully satisfy its optimal growth state, which is manifested in low reproductive capacity and low population expansion. On the other hand, for the acaroid mites with low reproductive ability such as Tyrophagous scavenging mite, Neoseiusi pasterei has a large food intake, and it is easy to eat up the food of Neoseius pasteruii such as Tyrosinus smoldering mite, and then starve to death. , making it impossible for the population of Neoseius pasterei to expand. For other acaroid mites, such as A. ellipsoids, although Neoseius ellipsoides can only eat the larvae of A. ellipsoids and cannot eat its adults, Neoseius ellipsoides cannot finish eating A. ellipsoids, but due to Its reproductive ability is low, and the population of Neoseiius pasterei is difficult to expand, the production cycle is long, and the yield is low. Ineffective invention application CN200580052561.5, "A mite composition comprising sweet mite species and phytoseioids" discloses that sweet mite is used as a host for raising phytoseioids, but sweet mite alone is used as prey to raise new spp. Seid mites cannot expand the population very well, and the artificial diet of sweet mites requires grain and sugar, which is expensive and is not a practical method.

Li Aihua et al. "Artificial breeding method for Amblyseius pasteurii" patent application CN200710006820.1 mentioned the use of grain fumigants, but according to our practice, grain fumigants are harmful to Neoseiius pasteurii, and the acarid mite therein It also has the characteristics of being unable to satisfy the food needs of Neoseius pasterii. Chen Chuan et al.’s invention application CN201310040281.9 for “Development-type breeding technology for Amblyseius pasterii” mentioned open-type breeding technology, but practice shows that a closed container with a net to ventilate but can prevent mites from running out is needed, and open-type breeding is easy. Pollution is not a practical technology.

At present, the artificial breeding method of Amblyseius pasteurii is still to raise acaroid mites such as Tyrophagophaera mite as feed mites to raise Neoseiius pasteurii, the yield is low, the production cycle is long, the population doubling time is long, and feeding will occur during the production process. The problem of population decline and unstable product quality has been unable to be effectively resolved, thus limiting the large-scale promotion and use of this Neoseius pasteurii product.

Contents of the invention

The technical problem mainly solved by the present invention is to provide a fast and efficient breeding method of Neoseius pastereii, using two different types of food mites, acaroid mites and sweet-eating mites, as common Neoseiusius pastereii Feed mite to feed Neoseius pastereius, can breed hundreds of millions of Neoseiius pastereius in large numbers, used as a variety of crops, fruits, vegetables on the natural enemy control spider mite, thrips, tarsus mite etc., the control effect is more than 90%, and the annual frequency of pesticide use is reduced by more than 60%, which can significantly increase crop yield and quantity, help protect the ecological environment, reduce pesticide residues, and help mass production of pollution-free food, vegetables, fruits and other green foods .

In order to solve the above-mentioned technical problems, a technical scheme adopted in the present invention is: a kind of fast and efficient breeding method of Neoseius pasterii is provided, comprising the following specific steps:

a. Preparation of artificial feed for feed mites, use wheat bran, grain, yeast and crop stalks to sterilize for 0.5-3 hours at a high temperature of 60-120 degrees Celsius for standby;

b. For the cultivation of feed mites, put the sterilized artificial feed of feed mites into the culture container, add water, the water content of the artificial feed of feed mites is 5-12%, and then add Acacia mites and sweet mite to cultivate the container for oral use Kraft paper or more than 300 meshes of gauze are closed, and the temperature is 15-35 degrees Celsius, and the relative humidity is 80-95% and cultivated for 10-30 days. When the density of the cultivated feed mites reaches 50-500 per gram, it is ready for use;

C, the seed production and cultivation of Neoseiius pasteurii, add the artificial feed of the sterilized feed mite in the culture container, add water, the water content of the artificial feed of the feed mite is controlled to be 5-12%, then add the feed mite and For Neoseiius pasteurii, the ratio of feed mite to Neoseiius pasteurii is 20-100:1, the culture container is closed with kraft paper or 300 mesh gauze, and the temperature is 15-35 degrees Celsius, and the relative humidity is 80-95%. Cultivate under the conditions for 30-50 days, and reach the density of the cultivated Neoseius pasteruii in the range of 10-30 per gram for later use;

d. For the industrial breeding of Neoseiius pasterii, do a good job in the disinfection of the breeding room, culture frame and culture container. Next, put the artificial feed of the sterilized feed mite into the culture container, add water, feed The water content of the artificial feed of mite is controlled to be 5-12%, then add Neoseiusius pasteurii and feed mite, the ratio of feed mite and Neoseius pasteurii is 20-100:1, culture container mouth uses kraft paper or 300-mesh gauze is sealed, and it is cultivated for 30-50 days at 15-35 degrees Celsius and relative humidity of 80-95%. When the number of Neoseiusius per gram reaches 20-40, it can be inspected and packaged.

In a preferred embodiment of the present invention, the grains include but not limited to rice flour, corn flour, wheat flour, rice, corn and wheat.

In a preferred embodiment of the present invention, the acaroid mite is T. ellipsoida or Tyrophagous rot; the sweet mite is sweet mite.

In a preferred embodiment of the present invention, the ratio of the acaroid mite and the sweet mite is 1:1.

In a preferred embodiment of the present invention, the temperatures in steps b, c and d are all 25 degrees centigrade, and the relative humidity is 85%.

The beneficial effect of the present invention is: a kind of fast and high-efficiency breeding method of Neoseius pasteurii of the present invention utilizes two kinds of food mites of different genera, acaroid mites and sweet mite The mite feed mite is used to feed Neoseius pasterei, which can breed hundreds of millions of Neoseius pastereius, which can be used as natural enemies on various crops, fruits and vegetables to prevent spider mites, thrips and tarsus Mites, etc., the control effect is more than 90%, and the annual frequency of pesticide use is reduced by more than 60%, which can significantly increase crop yield and quantity, help protect the ecological environment, reduce pesticide residues, and help mass production of pollution-free food, vegetables, fruits and other green food.

detailed description

The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Embodiments of the invention include:

A fast and efficient breeding method of Neoseius pasterii comprising the following specific steps:

a. Preparation of artificial feed for feed mites, use wheat bran, grain, yeast and crop stalks to sterilize for 0.5-3 hours at a high temperature of 60-120 degrees Celsius for standby;

b. For the cultivation of feed mites, put the sterilized artificial feed of feed mites into the culture container, add water, the water content of the artificial feed of feed mites is 5-12%, and then add Acacia mites and sweet mite to cultivate the container for oral use Kraft paper or more than 300 meshes of gauze are closed, and the temperature is 15-35 degrees Celsius, and the relative humidity is 80-95% and cultivated for 10-30 days. When the density of the cultivated feed mites reaches 50-500 per gram, it is ready for use;

C, the seed production and cultivation of Neoseiius pasteurii, add the artificial feed of the sterilized feed mite in the culture container, add water, the water content of the artificial feed of the feed mite is controlled to be 5-12%, then add the feed mite and For Neoseiius pasteurii, the ratio of feed mite to Neoseiius pasteurii is 20-100:1, the culture container is closed with kraft paper or 300 mesh gauze, and the temperature is 15-35 degrees Celsius, and the relative humidity is 80-95%. Cultivate under the conditions for 30-50 days, and reach the density of the cultivated Neoseius pasteruii in the range of 10-30 per gram for later use;

d. For the industrial breeding of Neoseiius pasterii, do a good job in the disinfection of the breeding room, culture frame and culture container. Next, put the artificial feed of the sterilized feed mite into the culture container, add water, feed The water content of the artificial feed of mite is controlled to be 5-12%, then add Neoseiusius pasteurii and feed mite, the ratio of feed mite and Neoseius pasteurii is 20-100:1, culture container mouth uses kraft paper or 300-mesh gauze is sealed, and it is cultivated for 30-50 days at 15-35 degrees Celsius and relative humidity of 80-95%. When the number of Neoseiusius per gram reaches 20-40, it can be inspected and packaged.

In the above, the grains include but are not limited to rice flour, corn flour, wheat flour, rice, corn and wheat; the ratio of the described meal mite and sweet mite is 1:1; the steps b, c and d in The temperature is 25 degrees Celsius and the relative humidity is 85%.

The acaroid mites refer to acaroid mites ellipsoids, tyrophagous tyrophagous mites, and acanthus roughipidae, and in particular acaroid mite ellipsoids and tyrophagous tyrophagous mites. Sweet mite refers to sweet mite, lepidophilus, etc., especially sweet mite. In this embodiment, the acaroid mite is A. ellipsoida or Tyrophagous rotifer, and the acaroid mite is sweet mite.

Feed mites can pass through grain and grain processing waste such as wheat bran, rice flour, rice, wheat bran, wheat, corn flour, corn, etc., especially the mixture of cheap wheat bran and a grain. Among them, crushed grain straw and vermiculite can be used as fillers, and yeast can also be added to increase nutrition, or not added.

Forage mites are defined as mites that can be used as feed for predator mites (here Neoseiius pasteurii). The acaroid mites refer to acaroid mites ellipsoids, tyrophagous tyrophagous mites, and acanthus roughipidae, and in particular acaroid mite ellipsoids and tyrophagous tyrophagous mites. Sweet mite refers to sweet mite, lepidophilus, etc., especially sweet mite.

Acaroid mites and sweet-eating mites are common harmful mites in grains, they can live in symbiosis at the same time, and they complement each other as the prey of Neoseius pasteurii. Sweet mite is characterized by strong population reproduction ability. Taking sweet mite as an example, at 25 degrees Celsius, the population doubling time is 2 days, the average generation cycle is 18 days, and the life cycle of female mite is 28 days. Among them, the spawning period is 19 days, and a total of 500 eggs are laid. Acaroid mites, for example, Acarina ellipsoida, are characterized by strong omnivorousness and good palatability to some cheap feeds such as wheat bran, but their reproductive ability is not as good as that of sweet mites. At 25 degrees Celsius, female mites lay eggs for 4-6 days , lay about 50 eggs in total, and complete a generation in about 16 days. Simple breeding of acaroid mites such as A. ellipsoides can be used to feed Neoseius pastereius with cheap feed, such as wheat bran, etc., but due to the insufficient reproductive capacity of acaroid mites, the food and nutrition of Neoseius pastereius are not sufficient, and cannot It satisfies the rapid reproduction of Neoseius pasterius very well. If N. pasteurii is only fed with sweet mite, it is characterized by rapid reproduction, but it needs to be fed with more expensive feed, such as rice flour, and N. pasterei is also partially nutrient deficient when fed with only sweet mite. lead to slow population growth. If the two are mixed, adopting a feed mixed with cheap wheat bran and grains such as rice flour can not only reduce the cost, but also meet the feeding needs of Neoseius pasteurii.

The above-mentioned ecological condition technology of required temperature, humidity and light conditions for artificial propagation is obtained through long-term exploration and practice. This method can breed hundreds of millions of a large number of Neoseius mite, which can be used as natural enemies on various crops, fruits, and vegetables to prevent and control spider mite, thrips, tarsus mite, etc., and the control effect is more than 90%. Reducing the frequency of pesticide use by more than 60% can significantly increase crop yield and quantity, help protect the ecological environment, reduce pesticide residues, and help mass production of pollution-free food, vegetables, fruits and other green foods.

This patent is further introduced in conjunction with the specific implementation mode below,

Implementation example 1:

Artificial feed preparation for feed mites (total 100 parts), 80 parts of wheat bran, 19.5 parts of rice flour, 0.5 part of yeast, mixed evenly, sterilized at 100 degrees Celsius for 30 minutes, cooled for later use.

All the breeding rooms and feeding containers were fully washed and disinfected for later use.

Feeding of feed mites: Add artificial feed into the culture container, add water so that the water content of the feed is 10%, add 1:1 ratio of ellipsoid and sweet mite. The initial density is 5-10 forage mites per gram of artificial feed. The mouth of the culture container was sealed with 300-mesh gauze. Put it into a culture room at 25 degrees Celsius and a humidity of 85%, and cultivate it for 30 days. The density of feed mites can reach 500 per gram of artificial feed.

Breeding of Neoseiusius mite species: artificial feed was added to the culture container, and water was added so that the water content of the feed was 10%. Add feed mites (1:1 ratio of ellipsoid and sweet mite), and the density of feed mites is about 200 per gram. Add Neoseius pasterei, and the density of Neoseius pasterei is about 4 heads per gram. The mouth of the culture container was sealed with 300-mesh gauze. Put it into a culture room at 25 degrees Celsius and a humidity of 85%, and cultivate it for 30 days. The density of Neoseius pasteruii can reach 40-50 heads per gram.

Industrial breeding of Neoseius pasterii: first do a good job of disinfection of the breeding room, culture racks and culture containers. The artificial feed was added to the culture container, and water was added so that the water content of the feed was 10%. Add feed mites (1:1 ratio of ellipsoid and sweet mite), and the density of feed mites is about 200 per gram. Add Neoseius pasterei, and the density of Neoseius pasterei is about 4 heads per gram. The mouth of the culture container was sealed with 300-mesh gauze. Put into 25 degrees centigrade, humidity 85% culture room culture. Every 10 days, the density of N. pasteurii was counted, compared with the artificial diet of the same formula and the same culture conditions. Density of mites. See Table 1 for statistical results. From Table 1, it can be seen that after 30 days of culture, Neoseiius pasteurii can be collected. At the same time, the effect of using A. ellipsoidal mite alone or Glycine domestica alone as feed mite to feed Neoseius pasterei was not satisfactory, showing that the population was difficult to expand, and the population decreased in the later stage.

Table 1. The results of feeding Neoseiius pasteurii with different feed mites:

.

Implementation example 2:

Artificial feed preparation for feed mites (total 100 parts), 85 parts of wheat bran, 14.5 parts of corn flour, 0.5 part of yeast, mixed evenly, sterilized at 100 degrees Celsius for 30 minutes, cooled for later use.

All the breeding rooms and feeding containers were fully washed and disinfected for later use.

Feeding of feed mites: Add artificial feed into the culture container, add water so that the water content of the feed is 10%, add 1:1 ratio of ellipsoid and sweet mite. The initial density is 5-10 forage mites per gram of artificial feed. The mouth of the culture container was sealed with 300-mesh gauze. Put it into a culture room at 25 degrees Celsius and a humidity of 85%, and cultivate it for 30 days. The density of feed mites can reach 500 per gram of artificial feed.

Breeding of Neoseiusius mite species: artificial feed was added to the culture container, and water was added so that the water content of the feed was 10%. Add feed mites (1:1 ratio of ellipsoid and sweet mite), and the density of feed mites is about 200 per gram. Add Neoseius pasterei, and the density of Neoseius pasterei is about 4 heads per gram. The mouth of the culture container was sealed with 300-mesh gauze. Put it into a culture room at 25 degrees Celsius and a humidity of 85%, and cultivate it for 30 days. The density of Neoseius pasteruii can reach 40-50 heads per gram.

Industrial breeding of Neoseius pasterii: first do a good job of disinfection of the breeding room, culture racks and culture containers. The artificial feed was added to the culture container, and water was added so that the water content of the feed was 10%. Add feed mites (1:1 ratio of ellipsoid and sweet mite), and the density of feed mites is about 200 per gram. Add Neoseius pasterei, and the density of Neoseius pasterei is about 4 heads per gram. The mouth of the culture container was sealed with 300-mesh gauze. Put into 25 degrees centigrade, humidity 85% culture room culture. Every 10 days, the density of N. pasteurii was counted, compared with the artificial diet of the same formula and the same culture conditions. Density of mites. See Table 2 for statistical results. From Table 2, it can be seen that after 30 days of cultivation, Neoseiius pasteurii can be collected. At the same time, the effect of using A. ellipsoidal mite alone or Glycine domestica alone as feed mite to feed Neoseius pasterei was not satisfactory, showing that the population was difficult to expand, and the population decreased in the later stage.

Table 2. The results of feeding Neoseiius pasteurii with different feed mites:

.

Implementation example 3:

Artificial feed preparation for feed mites (total 100 parts), 80 parts of wheat bran, 19.5 parts of wheat flour, 0.5 part of yeast, mixed evenly, sterilized at 100 degrees Celsius for 30 minutes, cooled for later use.

All the breeding rooms and feeding containers were fully washed and disinfected for later use.

Feed mite breeding: Add artificial feed into the culture container, add water so that the water content of the feed is 10%, add 1:1 ratio of tyrophagous mite and sweet mite. The initial density is 5-10 forage mites per gram of artificial feed. The mouth of the culture container was sealed with 300-mesh gauze. Put it into a culture room at 25 degrees Celsius and a humidity of 85%, and cultivate it for 30 days. The density of feed mites can reach 500 per gram of artificial feed.

Breeding of Neoseiusius mite species: artificial feed was added to the culture container, and water was added so that the water content of the feed was 10%. Add feed mites (1:1 ratio of tyrophagous mite and sweet mite), and the density of feed mite is about 200 per gram. Add Neoseius pasterei, and the density of Neoseius pasterei is about 4 heads per gram. The mouth of the culture container was sealed with 300-mesh gauze. Put it into a culture room at 25 degrees Celsius and a humidity of 85%, and cultivate it for 30 days. The density of Neoseius pasteruii can reach 40-50 heads per gram.

Industrial breeding of Neoseius pasterii: first do a good job of disinfection of the breeding room, culture racks and culture containers. The artificial feed was added to the culture container, and water was added so that the water content of the feed was 10%. Add feed mites (1:1 ratio of tyrophagous mite and sweet mite), and the density of feed mite is about 200 per gram. Add Neoseius pasterei, and the density of Neoseius pasterei is about 4 heads per gram. The mouth of the culture container was sealed with 300-mesh gauze. Put into 25 degrees centigrade, humidity 85% culture room culture. Every 10 days, the density of N. pasteurii was counted, and compared with the same formula of artificial diet and the same culture conditions, the density of N. saproseids or T. spp. was used alone as feed mite. Density of mites. See Table 3 for statistical results. From Table 3, it can be seen that after 30 days of cultivation, Neoseiius pasteruii can be collected. At the same time, the effect of feeding Neoseius pastereii with either Tyrophaerophagous mite alone or Sweet mite house-eating mite alone as feed mite was not satisfactory, showing that the population was difficult to expand, and the population decreased in the later stage.

Table 3. The results of feeding Neoseius pasteurii with different feed mites:

.

In summary, the fast and efficient breeding method of a kind of Neoseius pasteurii of the present invention utilizes two kinds of food mites of different genus, acaroid mites and sweet-eating mite, as common neoseiusius mite Feed mites are used to feed Neoseius pasterei, which can breed hundreds of millions of Neoseius pastereius, which can be used as natural enemies on various crops, fruits and vegetables to control red spiders, thrips, tarsus mites, etc. , the control effect is more than 90%, and the annual frequency of pesticide use is reduced by more than 60%, which can significantly increase crop yield and quantity, help protect the ecological environment, reduce pesticide residues, and help mass production of pollution-free food, vegetables, fruits and other green foods.

The above descriptions are only examples of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the content of the description of the present invention, or directly or indirectly used in other related technical fields, shall be The same reasoning is included in the patent protection scope of the present invention.

Claims (5)

1. the propagation method rapidly and efficiently of the new little peaceful mite of a kind of Pasteur, it is characterised in that including step in detail below：

A, the man-made feeds of forage mites are got the raw materials ready, with wheat bran, grain, yeast and crop material through 60-120 celsius temperatures Lower sterilization 0.5-3 hours are standby；

The man-made feeds of the forage mites sterilized are loaded culture vessel by b, the cultivation of forage mites, add water, forage mites it is artificial The water content of feed is 5-12%, adds culture vessel mouth brown paper after flour mite and the sweet mite of food or gauzes more than 300 mesh Closing, temperature be 15-35 degree Celsius, under conditions of relative humidity 80-95% cultivate 10-30 days, when cultivate forage mites it is close Degree is standby after reaching 50-500 per gram；

C, the production of hybrid seeds of the new little peaceful mite of Pasteur are cultivated, and add the man-made feeds of the forage mites sterilized, add water in culture vessel, The water content control of the man-made feeds of forage mites is 5-12%, adds forage mites and the new little peaceful mite of Pasteur, and forage mites and Pasteur are new The ratio of little peaceful mite is 20-100:1, the gauze closing of culture vessel brown paper or 300 mesh is that 15-35 is Celsius in temperature Degree, cultivates 30-50 days under conditions of relative humidity 80-95%, and the density for reaching the new little peaceful mite of Pasteur of cultivation is only every in 10-30 It is standby after gram scope；

D, the plant modification of the new little peaceful mite of Pasteur, carry out the disinfection of feeding room, culturing rack and culture vessel, next, The man-made feeds of sterilized forage mites are put in culture vessel, water, the water content control of the man-made feeds of forage mites is added For 5-12%, the ratio being subsequently adding as the new little peaceful mite of Pasteur and forage mites, forage mites and the new little peaceful mite of Pasteur is 20-100: 1, the gauze closing of culture vessel mouth brown paper or 300 mesh, at 15-35 degree Celsius, under conditions of relative humidity 80-95% Cultivate 30-50 days, after the quantity of the new little peaceful mite of every gram of Pasteur only reaches 20-40, you can pack after inspection.

2. the propagation method rapidly and efficiently of the new little peaceful mite of Pasteur according to claim 1, it is characterised in that described grain Food includes but is not limited to ground rice, corn flour, wheat flour, rice, corn and wheat.

3. the propagation method rapidly and efficiently of the new little peaceful mite of Pasteur according to claim 1, it is characterised in that described powder Mite is oval flour mite or tyrophagus putrescentiae；The sweet mite of described food is that sweet mite eats in family.

4. the propagation method rapidly and efficiently of the new little peaceful mite of Pasteur according to claim 1, it is characterised in that described powder The ratio of mite and the sweet mite of food is 1:1.

5. the propagation method rapidly and efficiently of the new little peaceful mite of Pasteur according to claim 1, it is characterised in that described step Temperature in rapid b, c and d is 25 degrees Celsius, and relative humidity is 85%.